@misc{davies_stankovic_azama_shibata_abe_2001, title={Novel components of the plant cytoskeleton: a beginning to plant 'cytomics'}, volume={160}, ISSN={["0168-9452"]}, DOI={10.1016/s0168-9452(00)00365-4}, abstractNote={The bulk of our knowledge concerning the plant cytoskeleton has come primarily from the use of techniques and probes derived from animal research. However, in comparison with animal tissues, relatively few plant cytoskeleton proteins have been identified. We presume this is not because the plant cytoskeleton is really made up of such few proteins, but rather that only rarely have attempts been made to identify plant-specific cytoskeleton proteins, using plant-specific methods. Here we outline methods that we have developed both for the isolation and identification of novel cytoskeleton proteins as well as for the visualization of novel filamentous structures in plant cells, and we describe several novel cytoskeleton proteins and two novel cytoskeleton structures, 'nanofilaments' and 'nanotubules'. We postulate that use of such approaches will lead to a rapid expansion of our knowledge of the plant cytoskeleton.}, number={2}, journal={PLANT SCIENCE}, author={Davies, E and Stankovic, B and Azama, K and Shibata, K and Abe, S}, year={2001}, month={Jan}, pages={185–196} }
 @article{stankovic_vian_henry-vian_davies_2000, title={Molecular cloning and characterization of a tomato cDNA encoding a systemically wound-inducible bZIP DNA-binding protein}, volume={212}, ISSN={["0032-0935"]}, DOI={10.1007/s004250000362}, abstractNote={Localized wounding of one leaf in intact tomato (Lycopersicon esculentum Mill.) plants triggers rapid systemic transcriptional responses that might be involved in defense. To better understand the mechanism(s) of intercellular signal transmission in wounded tomatoes, and to identify the array of genes systemically up-regulated by wounding, a subtractive cDNA library for wounded tomato leaves was constructed. A novel cDNA clone (designated LebZIP1) encoding a DNA-binding protein was isolated and identified. This clone appears to be encoded by a single gene, and belongs to the family of basic leucine zipper domain (bZIP) transcription factors shown to be up-regulated by cold and dark treatments. Analysis of the mRNA levels suggests that the transcript for LebZIP1 is both organ-specific and up-regulated by wounding. In wounded wild-type tomatoes, the LebZIP1 mRNA levels in distant tissue were maximally up-regulated within only 5 min following localized wounding. Exogenous abscisic acid (ABA) prevented the rapid wound-induced increase in LebZIP1 mRNA levels, while the basal levels of LebZIP1 transcripts were higher in the ABA mutants notabilis (not), sitiens (sit), and flacca (flc), and wound-induced increases were greater in the ABA-deficient mutants. Together, these results suggest that ABA acts to curtail the wound-induced synthesis of LebZIP1 mRNA.}, number={1}, journal={PLANTA}, author={Stankovic, B and Vian, A and Henry-Vian, C and Davies, E}, year={2000}, month={Dec}, pages={60–66} }
 @article{shibata_morita_abe_stankovic_davies_1999, title={Apyrase from pea stems: Isolation, purification, characterization and identification of a NTPase from the cytoskeleton fraction of pea stem tissue}, volume={37}, ISSN={["0981-9428"]}, DOI={10.1016/S0981-9428(99)00102-3}, abstractNote={The cytoskeleton pellet from the first internode of dark-grown pea stems was disintegrated in a high salt buffer, ultracentrifuged to remove ribosomes and the post-ribosomal supernatant was applied to a heparin affinity column. Significant ATPase activity was present in the cytoskeleton fraction and this was eluted from the column at 0.6–0.7 M KOAc, in the same fractions as a 49-kDa protein (which we called B3). B3 was desalted and further purified by cation exchange column chromatography. Purified B3 catalyzed hydrolysis of ATP, CTP, GTP, TTP, UTP and ADP and thus appears to be an apyrase (ATP diphosphohydrolase, EC 3.6.1.5). Partial amino acid sequences of three major fragments were obtained by digestion of B3 by Staphylococcus aureus V8 protease (EC 3.4.21.19), and all these sequences were consistent with the previously reported amino acid sequences for pea nucleoside triphosphatase (NTPase, EC 3.6.1.15) (PIR S48859), which is thought to be an apyrase.}, number={12}, journal={PLANT PHYSIOLOGY AND BIOCHEMISTRY}, author={Shibata, K and Morita, Y and Abe, S and Stankovic, B and Davies, E}, year={1999}, month={Dec}, pages={881–888} }
 @article{stankovic_abe_azama_shibata_ito_weidner_davies_1999, title={Associations of maize protein bodies with cytoskeleton, membranes, and ribosomes in the endosperm of wild type and opaque-2 mutant}, volume={21}, ISSN={["0137-5881"]}, DOI={10.1007/s11738-999-0010-3}, number={4}, journal={ACTA PHYSIOLOGIAE PLANTARUM}, author={Stankovic, B and Abe, S and Azama, K and Shibata, K and Ito, Y and Weidner, S and Davies, E}, year={1999}, pages={383–389} }
 @article{stankovic_witters_zawadzki_davies_1998, title={Action potentials and variation potentials in sunflower: An analysis of their relationships and distinguishing characteristics}, volume={103}, ISSN={["1399-3054"]}, DOI={10.1034/j.1399-3054.1998.1030107.x}, abstractNote={Sunflower plants (Helianthus annuus L.) were given an electrical stimulus to the stem or a heat (flame)‐wound to a single leaf or a cotyledon. The resulting electrical activity was monitored with extracellular electrodes. An electrical stimulus applied to the stem frequently evoked an action potential (AP), but never a variation potential (VP). In contrast, a heat‐wound applied to a leaf virtually always elicited a VP, which was often accompanied by one or more superimposed spikes (putative APs). The kinetic parameters of the AP and the VP were investigated. The AP appears to propagate without decrement in velocity or magnitude, whereas the VP parameters decrease significantly with distance. The heat stimulus triggered rapid alterations in stem elongation/contraction, which preceded changes in electrical potential, indicating the transmission of a hydraulic signal. Light‐off and light‐on stimuli evoked negative‐ and positive‐going changes in extracellular electrical potential, respectively, corresponding to de‐ and hyper‐polarization of the plasma membrane. Membrane depolarization (extracellularly manifested as a VP) evoked by both the light‐off and heat‐wounding stimuli was able to trigger one or more APs. We interpret these results to suggest that APs are “genuine” electrical signals involving voltage‐gated ion channels or pumps, which can be evoked directly by electrical stimulation or indirectly by changes in membrane potential occurring during the VP or after the light‐off stimulus. In contrast, VPs appear to be a local (non‐transmissible) electrical consequence of the passage of a rapidly transmitted hydraulic signal in the xylem, presumably acting on mechanosensitive ion channels or pumps in adjacent living cells.}, number={1}, journal={PHYSIOLOGIA PLANTARUM}, author={Stankovic, B and Witters, DL and Zawadzki, T and Davies, E}, year={1998}, month={May}, pages={51–58} }
 @article{stankovic_volkmann_sack_1998, title={Autonomic straightening after gravitropic curvature of cress roots}, volume={117}, ISSN={["0032-0889"]}, DOI={10.1104/pp.117.3.893}, abstractNote={Abstract}, number={3}, journal={PLANT PHYSIOLOGY}, author={Stankovic, B and Volkmann, D and Sack, FD}, year={1998}, month={Jul}, pages={893–900} }
 @article{stankovic_davies_1998, title={The wound response in tomato involves rapid growth and electrical responses, systemically up-regulated transcription of proteinase inhibitor and calmodulin and down-regulated translation}, volume={39}, ISSN={["0032-0781"]}, DOI={10.1093/oxfordjournals.pcp.a029367}, abstractNote={Localized heat wounding of tomato plants triggered rapid changes in growth and in electrical activity. The growth alterations were manifested as a transient increase in growth (petiole elongation), followed by a massive, long-lasting growth reduction. The electrical potential changes consisted of a wave of depolarization and re-polarization, i.e., a variation potential (VP). The tissue deformation apparently resulted from a pressure surge rapidly transmitted through the xylem, and preceded the changes in electrical potential. Externally-applied pressure mimicked flame wounding by triggering an electrical response resembling a VP. Our findings suggest that the VP results from a pressure surge in the xylem causing change in activity of mechanosensitive ion channels or pumps in adjacent living cells. The ensuing ion fluxes evoke plasma membrane depolarization, monitored extracellularly as a VP. Wounding also evoked a systemic decrease in polysomes, as well as a decrease in their protein synthesizing capacity in vitro. Very little of the newly-synthesized proteinase inhibitor (pin) and calmodulin (cal) mRNA was recruited into polysomes during the first hour following wounding. Since the VP appearance in distant tissue preceded the systemic molecular responses, the VP might be the long-distance signal up-regulating transcription of proteinase inhibitors and calmodulin, and down-regulating translation.}, number={3}, journal={PLANT AND CELL PHYSIOLOGY}, author={Stankovic, B and Davies, E}, year={1998}, month={Mar}, pages={268–274} }
 @article{stankovic_zawadzki_davies_1997, title={Characterization of the variation potential in sunflower}, volume={115}, ISSN={["1532-2548"]}, DOI={10.1104/pp.115.3.1083}, abstractNote={Abstract}, number={3}, journal={PLANT PHYSIOLOGY}, author={Stankovic, B and Zawadzki, T and Davies, E}, year={1997}, month={Nov}, pages={1083–1088} }
 @article{davies_vian_vian_stankovic_1997, title={Rapid systemic up-regulation of genes after heat-wounding and electrical stimulation}, volume={19}, ISSN={["0137-5881"]}, DOI={10.1007/s11738-997-0055-0}, abstractNote={When one leaf of a tomato plant is electrically-stimulated or heat-wounded, proteinase inhibitor genes are rapidly up-regulated in distant leaves. The identity of the systemic wound signal(s) is not yet known, but major candidates include hormones transmitted via the phloem or the xylem, the electrically-stimulated self-propagating electrical signal in the phloem (the action potential, AP), or the heat-wound-induced surge in hydraulic pressure in the xylem evoking a local change in membrane potential in adjacent living cells (the variation potential, VP). In order to discriminate between these signals we have adopted two approaches. The first approach involves applying stimuli that evoke known signals and determining whether these signals have similar effects on the "model" transcripts for proteinase inhibitors (pin) and calmodulin (cal). Here we show that a heat wound almost invariably evokes a VP, while an electrical stimulation occasionally evokes an AP, and both of these signals induce accumulation of transcripts encoding proteinase inhibitors. The second approach involves identifying the array of genes turned on by heat-wounding. To this end, we have constructed a subtractive library for heat-wounded tissue, isolated over 800 putatively up-regulated clones, and shown that all but two of the fifty that we have analyzed by Northern hybridization are, indeed, up-regulated. Here we show the early kinetics of up-regulation of three of these transcripts in the terminal (4th) leaf in response to heat-wounding the 3rd leaf, about 5 cm away. Even though these transcripts show somewhat different time courses of induction, with one peaking at 30 min, another at 15 min, and another at 5 min after flaming of a distant leaf, they all exhibit a similar pattern, i.e., a transient period of transcript accumulation preceding a period of transcript decrease, followed by a second period of transcript accumulation.}, number={4}, journal={ACTA PHYSIOLOGIAE PLANTARUM}, author={Davies, E and Vian, A and Vian, C and Stankovic, B}, year={1997}, pages={571–576} }