@misc{franzen_feldheim_tkachenko_godek_ryan_anderson_2008, title={Nanoparticle delivery vehicle}, volume={7,332,586}, number={2008 Feb. 19}, publisher={Washington, DC: U.S. Patent and Trademark Office}, author={Franzen, S. and Feldheim, D. L. and Tkachenko, A. G. and Godek, M. L. and Ryan, J. A. and Anderson, M. F.}, year={2008} }
 @article{zeng_ballard_tkachenko_burns_feldheim_melander_2006, title={Mimicking the biological activity of diazobenzo[b]fluorene natural products with electronically tuned diazofluorene analogs}, volume={16}, ISSN={["0960-894X"]}, DOI={10.1016/j.bmcl.2006.07.024}, abstractNote={Under appropriate electronic modulation, simple diazofluorene analogs recapitulate the DNA cleavage activity of kinamycin D under thiol-based reducing conditions. Achieving DNA cleavage under these reducing conditions is key to anticancer activity, as the most active compound, 1-methoxydiazofluorene, inhibits the proliferation of HeLa cells.}, number={19}, journal={BIOORGANIC & MEDICINAL CHEMISTRY LETTERS}, author={Zeng, Wei and Ballard, T. Eric and Tkachenko, Alexander G. and Burns, Virginia A. and Feldheim, Daniel L. and Melander, Christian}, year={2006}, month={Oct}, pages={5148–5151} }
 @article{liu_gugliotti_wu_dolska_tkachenko_shipton_eaton_feldheim_2006, title={RNA-mediated synthesis of palladium nanoparticles on Au surfaces}, volume={22}, ISSN={["0743-7463"]}, DOI={10.1021/la060426c}, abstractNote={RNA catalysts for the shape-controlled synthesis of Pd particles from the precursor complex trisdibenzylideneacetone dipalladium ([Pd2(DBA)3] were recently discovered in our laboratory (J. Am. Chem. Soc. 2005, 127, 17814-17818). In the work described here, RNA codes for hexagonal Pd platelets and Pd cubes were covalently immobilized on gold surfaces and evaluated for their activity toward particle synthesis. When coupled to gold via oligoethylene glycol linkers, both RNA sequences were able to catalyze the formation of Pd particles with the same shape control previously observed in solution. For low surface coverages, the average distance between RNA molecules on the surface was estimated at ca. 300 nm, yet large (e.g., dimensions of hundreds of nanometers) Pd hexagons and cubes still formed. This surprising result suggests that a single RNA molecule may be sufficient for nucleating and controlling the shapes of these particles. Finally, the use of surface-bound RNA as a tool for directing the orthogonal synthesis of materials on surfaces was demonstrated. Patterning the RNA code for Pd hexagons next to the code for Pd cubes, followed by incubation in a solution containing [Pd2(DBA)3], resulted in the spontaneous formation of spatially distinct spots of hexagonal and cubic particles.}, number={13}, journal={LANGMUIR}, author={Liu, Dage and Gugliotti, Lina A. and Wu, Tong and Dolska, Magda and Tkachenko, Alexander G. and Shipton, Mathew K. and Eaton, Bruce E. and Feldheim, Daniel L.}, year={2006}, month={Jun}, pages={5862–5866} }
 @article{tkachenko_xie_liu_coleman_ryan_glomm_shipton_franzen_feldheim_2004, title={Cellular trajectories of peptide-modified gold particle complexes: Comparison of nuclear localization signals and peptide transduction domains}, volume={15}, ISSN={["1520-4812"]}, DOI={10.1021/bc034189q}, abstractNote={Gold nanoparticles modified with nuclear localization peptides were synthesized and evaluated for their subcellular distribution in HeLa human cervical epithelium cells, 3T3/NIH murine fibroblastoma cells, and HepG2 human hepatocarcinoma cells. Video-enhanced color differential interference contrast microscopy and transmission electron microscopy indicated that transport of nanoparticles into the cytoplasm and nucleus depends on peptide sequence and cell line. Recently, the ability of certain peptides, called protein transduction domains (PTDs), to transclocate cell and nuclear membranes in a receptor- and temperature-independent manner has been questioned (see for example, Lundberg, M.; Wikstrom, S.; Johansson, M. (2003) Mol. Ther. 8, 143-150). We have evaluated the cellular trajectory of gold nanoparticles carrying the PTD from HIV Tat protein. Our observations were that (1) the conjugates did not enter the nucleus of 3T3/NIH or HepG2 cells, and (2) cellular uptake of Tat PTD peptide-gold nanoparticle conjugates was temperature dependent, suggesting an endosomal pathway of uptake. Gold nanoparticles modified with the adenovirus nuclear localization signal and the integrin binding domain also entered cells via an energy-dependent mechanism, but in contrast to the Tat PTD, these signals triggered nuclear uptake of nanoparticles in HeLa and HepG2 cell lines.}, number={3}, journal={BIOCONJUGATE CHEMISTRY}, author={Tkachenko, AG and Xie, H and Liu, YL and Coleman, D and Ryan, J and Glomm, WR and Shipton, MK and Franzen, S and Feldheim, DL}, year={2004}, pages={482–490} }
 @article{kramer_xie_gaff_williamson_tkachenko_nouri_feldheim_feldheim_2004, title={Preparation of protein gradients through the controlled deposition of protein-nanoparticle conjugates onto functionalized surfaces}, volume={126}, ISSN={["0002-7863"]}, DOI={10.1021/ja031674n}, abstractNote={This paper describes a simple method for the preparation and characterization of protein density gradients on solid supports. The method employs colloidal metal nanoparticles as protein carriers and optical tags and is capable of forming linear, exponential, 1D, 2D, and multiprotein gradients of varying slope without expensive or sophisticated surface patterning techniques. Surfaces patterned with proteins using the procedures described within are shown to support cell growth and are thus suitable for studies of protein-cell interactions.}, number={17}, journal={JOURNAL OF THE AMERICAN CHEMICAL SOCIETY}, author={Kramer, S and Xie, H and Gaff, J and Williamson, JR and Tkachenko, AG and Nouri, N and Feldheim, DA and Feldheim, DL}, year={2004}, month={May}, pages={5388–5395} }
 @article{xie_tkachenko_glomm_ryan_brennaman_papanikolas_franzen_feldheim_2003, title={Critical flocculation concentrations, binding isotherms, and ligand exchange properties of peptide-modified gold nanoparticles studied by UV-visible, fluorescence, and time-correlated single photon counting spectroscopies}, volume={75}, ISSN={["0003-2700"]}, DOI={10.1021/ac034578d}, abstractNote={Protocols for modifying gold nanoparticles with peptide-bovine serum albumin (BSA) conjugates are described within. The resulting constructs were characterized using a number of techniques including static fluorescence spectroscopy and time-correlated single photon counting spectroscopy (TCSPC) in order to quantify peptide-BSA binding isotherms, exchange rates, critical flocculation concentrations, and the composition of mixed peptide-BSA monolayers on gold nanoparticles. TCSPC has proven to be a powerful technique for observing the microenvironment of protein-gold nanoparticle conjugates because it can distinguish between surface-bound and solution-phase species without the need for separation steps. Full characterization of the composition and stability of peptide-modified metal nanoparticles is an important step in their use as intracellular delivery vectors and imaging agents.}, number={21}, journal={ANALYTICAL CHEMISTRY}, author={Xie, H and Tkachenko, AG and Glomm, WR and Ryan, JA and Brennaman, MK and Papanikolas, JM and Franzen, S and Feldheim, DL}, year={2003}, month={Nov}, pages={5797–5805} }