@article{ross_buntzman_vincent_grover_gojanovich_collins_frelinger_hess_2012, title={Allelic diversity at the DLA-88 locus in Golden Retriever and Boxer breeds is limited}, volume={80}, ISSN={["1399-0039"]}, url={http://europepmc.org/articles/PMC3407292}, DOI={10.1111/j.1399-0039.2012.01889.x}, abstractNote={In the dog, previous analyses of major histocompatibility complex class I genes suggest a single polymorphic locus, dog leukocyte antigen (DLA)‐88. While 51 alleles have been reported, estimates of prevalence have not been made. We hypothesized that, within a breed, DLA‐88 diversity would be restricted, and one or more dominant alleles could be identified. Accordingly, we determined allele usage in 47 Golden Retrievers and 39 Boxers. In each population, 10 alleles were found; 4 were shared. Seven novel alleles were identified. DLA‐88*05101 and *50801 predominated in Golden Retrievers, while most Boxers carried *03401. In these breeds, DLA‐88 polymorphisms are limited and largely non‐overlapping. The finding of highly prevalent alleles fulfills an important prerequisite for studying canine CD8+ T‐cell responses.}, number={2}, journal={TISSUE ANTIGENS}, author={Ross, P. and Buntzman, A. S. and Vincent, B. G. and Grover, E. N. and Gojanovich, G. S. and Collins, E. J. and Frelinger, J. A. and Hess, P. R.}, year={2012}, month={Aug}, pages={175–183} } @article{harms_papich_stamper_ross_rodriguez_hohn_2004, title={Pharmacokinetics of oxytetracycline in loggerhead sea turtles (Caretta caretta) after single intravenous and intramuscular injections}, volume={35}, ISSN={["1042-7260"]}, DOI={10.1638/03-083}, abstractNote={Abstract The pharmacokinetics of oxytetracycline in 2-yr-old loggerhead sea turtles (Caretta caretta) after single i.v. and i.m. injections were studied for biologic marking and therapeutic applications. Twenty juvenile turtles were divided into two treatment groups. Ten animals received 25 mg/kg of oxytetracycline i.v. and 10 received the same dosage i.m. Plasma oxytetracycline concentrations were analyzed by reverse-phase high-performance liquid chromatography. Data from the i.v. route best fit a three-compartment model, whereas noncompartmental analysis was used to compare data from both the i.v. and i.m routes. For the i.v. route, means for maximum plasma concentration, terminal phase half-life, systemic clearance, and apparent volume of distribution at steady state were 6.6 μg/ml, 66.1 hr, 290.7 ml/hr/kg, and 18.4 L, respectively. For the i.m. route, means for systemic availability, maximum plasma concentration, and elimination half-life were 91.8%, 1.6 μg/ml, and 61.9 hr, respectively. The remarkably high apparent volume of distribution may possibly be associated with a deep compartment of drug disposition such as bone deposition associated with the large skeletal mass of turtles and the fact that these were well-nourished, growing juveniles. Although maximum plasma concentration by i.m. administration was lower than for the i.v. route, the long elimination time indicates that an infrequent dosing interval may be effective for sensitive bacteria.}, number={4}, journal={JOURNAL OF ZOO AND WILDLIFE MEDICINE}, author={Harms, CA and Papich, MG and Stamper, A and Ross, PM and Rodriguez, MX and Hohn, AA}, year={2004}, month={Dec}, pages={477–488} } @article{harms_mallo_ross_segars_2003, title={Venous blood gases and lactates of wild loggerhead sea turtles (Caretta caretta) following two capture techniques}, volume={39}, ISSN={["1943-3700"]}, DOI={10.7589/0090-3558-39.2.366}, abstractNote={During summer of 2001, venous blood gases were determined in loggerhead sea turtles (Caretta caretta) captured by trawl (n=16) in coastal waters of South Carolina and Georgia (USA) as part of a sea turtle census program and captured in pound nets (n=6) in coastal North Carolina (USA) during a study of sea turtle population biology. Trawls were towed for 30 min, so turtles captured were forcibly submerged for ≤30 min. Pound nets are passive gear in which fish and sea turtles are funneled into a concentrated area and removed periodically. Sea turtles in pound nets are free to surface and to feed at will. Blood was obtained from the dorsal cervical sinus as quickly as possible after landing on the boat (range 2–10 min trawl, 1–2 min pound net) and at 30 min after landing just prior to release. Blood gases including pH, partial pressures of O2 and CO2 (pO2, pCO2), and lactate were measured within 10 min. Instrument measurements for pH, pO2, and pCO2 made at 37 C were corrected to cloacal temperature and HCO3− was calculated from temperature-corrected pH and pCO2. Venous blood pH and bicarbonate were higher, and pO2 and lactate were lower from pound net-captured turtles compared to trawl captured turtles at the initial sampling time. In pound net turtles, pH and bicarbonate declined and lactate increased during 30 min on deck. In trawled sea turtles, venous blood pH increased and pCO2 and pO2 decreased during the 30 min on deck. Both capture systems caused perturbations in blood gas, acid-base, and lactate status, though alterations were greater in trawl captured turtles.}, number={2}, journal={JOURNAL OF WILDLIFE DISEASES}, author={Harms, CA and Mallo, KM and Ross, PM and Segars, A}, year={2003}, month={Apr}, pages={366–374} }