@article{mozdziak_wu_bradford_pardue_borwornpinyo_giamario_petitte_2006, title={Identification of the lacZ insertion site and beta-galactosidase expression in transgenic chickens}, volume={324}, ISSN={["1432-0878"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-33644623527&partnerID=MN8TOARS}, DOI={10.1007/s00441-005-0060-9}, abstractNote={The quail:chick chimera system is a classical research model in developmental biology. An improvement over the quail:chick chimera system would be a line of transgenic chickens expressing a reporter gene. Transgenic chickens carrying lacZ and expressing bacterial beta-galactosidase have been generated, but complete characterization of the insertion event and characterization of beta-galactosidase expression have not previously been available. The genomic sequences flanking the retroviral insertion site have now been identified by using inverse polymerase chain reaction (PCR), homozygous individuals have been identified by using PCR-based genotyping, and beta-galactosidase expression has been evaluated by using Western analysis and histochemistry. Based upon the current draft of the chicken genome, the viral insertion carrying the lacZ gene has been located on chromosome 11 within the predicted gene for neurotactin/fractalkine (CX3CL1); neurotactin mRNA expression appears to be missing from the brain of homozygous individuals. When Generation 2 (G2) lacZ-positive individuals were inter-mated, they generated 361 G3 progeny; 82 were homozyous for lacZ (22.7%), 97 were wild-type non-transgenic (26.9%), and 182 (50.4%) were hemizygous for lacZ. Western analysis revealed the highest expression in the muscle and liver. With the identification of homozygous birds, the line of chickens is now designated NCSU-Blue1.}, number={1}, journal={CELL AND TISSUE RESEARCH}, author={Mozdziak, PE and Wu, Q and Bradford, JM and Pardue, SL and Borwornpinyo, S and Giamario, C and Petitte, JN}, year={2006}, month={Apr}, pages={41–53} } @article{mozdziak_giamario_dibner_mccoy_2004, title={A chicken mRNA similar to heterogeneous nuclear ribonucleoprotein H1}, volume={137}, ISSN={["1879-1107"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-0346157313&partnerID=MN8TOARS}, DOI={10.1016/j.cbpc.2003.10.007}, abstractNote={Heterogeneous nuclear ribonucleoproteins are predominantly nuclear RNA-binding proteins that function in a variety of cellular activities. The objective of these experiments was to clone a cDNA for a chicken protein similar to other previously reported heterogeneous ribonucleoproteins for other species. The 5' and 3' ends of the chicken mRNA were cloned using Rapid Amplification of cDNA Ends (RACE). Subsequently, the expression of the mRNA sequence was confirmed via Northern analysis. The deduced amino acid sequence was approximately 86% identical to corresponding regions of human, mouse, or zebrafish proteins similar to heterogeneous nuclear ribonucleoprotein H1. The expression data confirmed the size of the predicted mRNA sequence. The newly identified sequence may be employed in future studies aimed at understanding the role of heterogeneous nuclear ribonucleoproteins in avian species.}, number={1}, journal={COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY}, author={Mozdziak, PE and Giamario, C and Dibner, JJ and McCoy, DW}, year={2004}, month={Jan}, pages={89–94} } @article{giamario_petitte_mozdziak_2003, title={Hatchability of chicken embryos following somite manipulation}, volume={34}, ISSN={["1940-9818"]}, url={http://europepmc.org/abstract/med/12813875}, DOI={10.2144/03346bm01}, abstractNote={BioTechniquesVol. 34, No. 6 BenchmarksOpen AccessHatchability of Chicken Embryos Following Somite ManipulationCarol Giamario, James N. Petitte & Paul E. MozdziakCarol GiamarioNorth Carolina State University, Raleigh, NC, USASearch for more papers by this author, James N. PetitteNorth Carolina State University, Raleigh, NC, USASearch for more papers by this author & Paul E. Mozdziak*Address correspondence to Dr. Paul Mozdziak, Department of Poultry Science, North Carolina State University, Campus Box 7608/Scott Hall, Raleigh, NC 27695, USA. e-mail: E-mail Address: pemozdzi@unity.ncsu.eduNorth Carolina State University, Raleigh, NC, USASearch for more papers by this authorPublished Online:24 Sep 2018https://doi.org/10.2144/03346bm01AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinkedInReddit FiguresReferencesRelatedDetailsCited ByNew methods for chicken embryo manipulationsMicroscopy Research and Technique, Vol. 21Identification of the lacZ insertion site and beta-galactosidase expression in transgenic chickens12 January 2006 | Cell and Tissue Research, Vol. 324, No. 1Avian Genetic Resource Banking: Can Fish Embryos Yield Any Clues for Bird Embryos?Poultry Science, Vol. 85, No. 2In ovo intraperitoneal administration of bromodeoxyuridine to avian fetusesDaniel T. Moore, Peter R. Ferket & Paul E. Mozdziak6 June 2018 | BioTechniques, Vol. 36, No. 1 Vol. 34, No. 6 Follow us on social media for the latest updates Metrics Downloaded 217 times History Published online 24 September 2018 Published in print June 2003 Information© 2018 Author(s)PDF download}, number={6}, journal={BIOTECHNIQUES}, author={Giamario, C and Petitte, JN and Mozdziak, PE}, year={2003}, month={Jun}, pages={1128–1130} } @article{giamario_petitte_mozdziak_2003, title={Myonuclear accrestion-a brief review}, volume={21}, number={Suppl. 1}, journal={Animal Science Papers and Reports}, author={Giamario, C. and Petitte, J. N. and Mozdziak, P. E.}, year={2003}, pages={121–131} }