Matt D. Halfhill

Works (5)

Updated: July 5th, 2023 15:59

2005 journal article

Growth, productivity, and competitiveness of introgressed weedy Brassica rapa hybrids selected for the presence of Bt cry1Ac and gfp transgenes

MOLECULAR ECOLOGY, 14(10), 3177–3189.

By: M. Halfhill n, J. Sutherland*, H. Moon*, G. Poppy *, S. Warwick*, A. Weissinger n, T. Rufty n, P. Raymer *, C. Stewart *

author keywords: Bacillus thuringiensis; Brassica napus; Brassica rapa; hybridization; insect resistance; introgression
MeSH headings : Bacillus thuringiensis Toxins; Bacterial Proteins / genetics; Bacterial Toxins / genetics; Brassica napus / genetics; Brassica napus / growth & development; Brassica rapa / genetics; Brassica rapa / growth & development; Crosses, Genetic; Endotoxins / genetics; Green Fluorescent Proteins / genetics; Hemolysin Proteins; Plant Leaves / growth & development; Plants, Genetically Modified / genetics; Plants, Genetically Modified / growth & development; Random Allocation; Triticum / growth & development
TL;DR: The hybrids were the least competitive with wheat compared with parental Brassica competitors, although differences between transgenic and nontransgenic hybrids varied with location. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2004 journal article

Hybridization and backcrossing between transgenic oilseed rape and two related weed species under field conditions

Environmental Biosafety Research, 3(2), 73.

By: M. Halfhill n, W. Zhu Bin, P. Raymer *, R. Millwood *, A. Weissinger n & C. Stewart

MeSH headings : Agriculture; Bacillus thuringiensis / genetics; Bacillus thuringiensis / pathogenicity; Brassica napus / genetics; Brassica rapa / genetics; Green Fluorescent Proteins / genetics; Hybridization, Genetic; Plants, Genetically Modified; Raphanus / genetics; Risk Assessment; Safety; Seeds / genetics
TL;DR: Results show that transgene flow from many independent transformed lines of B. rapa can occur under a range of field conditions, and that transgenic hybrids have a high potential to produce transgenic seeds in backcrosses. (via Semantic Scholar)
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Source: NC State University Libraries
Added: August 6, 2018

2003 journal article

Additive transgene expression and genetic introgression in multiple green-fluorescent protein transgenic crop x weed hybrid generations

THEORETICAL AND APPLIED GENETICS, 107(8), 1533–1540.

By: M. Halfhill n, R. Millwood *, A. Weissinger n, S. Warwick* & C. Stewart *

author keywords: transgenic canola; Brassica rapa; green fluorescent protein; AFLPs; gene flow
MeSH headings : Crops, Agricultural / genetics; Crosses, Genetic; Green Fluorescent Proteins; Luminescent Proteins / genetics; Plants, Genetically Modified; Spectrometry, Fluorescence; Transgenes
TL;DR: It is demonstrated that the formation of homozygous individuals within hybrid populations increases the average level of transgene expression as generations progress, and this phenomenon must be considered in the development of risk-management strategies. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Quantitative GFP fluorescence as an indicator of recombinant protein synthesis in transgenic plants

PLANT CELL REPORTS, 22(2), 117–121.

By: H. Richards*, M. Halfhill n, R. Millwood * & C. Stewart *

author keywords: fluorescence; GFP; protein quantification; plant transformation; ELISA
MeSH headings : Enzyme-Linked Immunosorbent Assay / methods; Green Fluorescent Proteins; Luminescent Proteins / genetics; Luminescent Proteins / metabolism; Plant Proteins / genetics; Plant Proteins / metabolism; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / metabolism; Spectrometry, Fluorescence / methods
TL;DR: Fluorescence intensity was linear with increasing levels of GFP over a range that encompasses transgene expression in plants by the cauliflower mosaic virus 35S promoter, indicating that the technique is a reliable measure of recombinant GFP expression. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Spatial and temporal patterns of green fluorescent protein (GFP) fluorescence during leaf canopy development in transgenic oilseed rape, Brassica napus L.

PLANT CELL REPORTS, 22(5), 338–343.

By: M. Halfhill n, R. Millwood *, T. Rufty n, A. Weissinger n & C. Stewart *

author keywords: green fluorescent protein; Bacillus thuringiensis; soluble protein concentration; transgene monitoring; canola
MeSH headings : Bacillus thuringiensis Toxins; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Bacterial Toxins; Brassica napus / genetics; Brassica napus / growth & development; Brassica napus / metabolism; Endotoxins / genetics; Endotoxins / metabolism; Fluorescence; Green Fluorescent Proteins; Hemolysin Proteins; Luminescent Proteins / genetics; Luminescent Proteins / metabolism; Plant Leaves / genetics; Plant Leaves / growth & development; Plant Leaves / metabolism; Plant Proteins / genetics; Plant Proteins / metabolism; Plants, Genetically Modified / genetics; Plants, Genetically Modified / growth & development; Plants, Genetically Modified / metabolism; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / metabolism
TL;DR: The results show that GFP can be used as an accurate transgene marker but that aspects of plant developmental should be taken into account when interpreting fluorescence measurements. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

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