Works (2)

Updated: July 5th, 2023 15:58

2004 journal article

Multiplex nucleic acid sequence-based amplification for simultaneous detection of several enteric viruses in model ready-to-eat foods


By: J. Jean n, . DH D'Souza & L. Jaykus n

MeSH headings : Animals; Food Contamination; Hepatitis A virus / genetics; Hepatitis A virus / isolation & purification; Humans; Lettuce / microbiology; Meat Products / microbiology; Norovirus / genetics; Norovirus / isolation & purification; Polymerase Chain Reaction; Self-Sustained Sequence Replication / methods
TL;DR: The multiplex NASBA system provides rapid and simultaneous detection of clinically relevant food-borne viruses in a single reaction tube and may be a promising alternative to reverse transcription-PCR for the detection of viral contamination of foods. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Transcriptional enhancement of RT-PCR for rapid and sensitive detection of Noroviruses


By: J. Jean n, . D D'Souza & L. Jaykus n

author keywords: Norovirus; Norwalk-like virus; enteric virus; RT-PCR; transcriptional enhancement
MeSH headings : Environmental Microbiology; Food Microbiology; Norovirus / genetics; Norovirus / isolation & purification; Nucleic Acid Hybridization; Reproducibility of Results; Reverse Transcriptase Polymerase Chain Reaction / methods; Self-Sustained Sequence Replication / methods; Sensitivity and Specificity; Transcription, Genetic
TL;DR: The NASBA-derived primers performed comparably to other broadly reactive GII Norovirus primers with respect to detection limits and shows promise for improved detection sensitivity in instances where very low levels of virus contamination might be anticipated. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

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