@article{jean_dh d'souza_jaykus_2004, title={Multiplex nucleic acid sequence-based amplification for simultaneous detection of several enteric viruses in model ready-to-eat foods}, volume={70}, ISSN={["0099-2240"]}, DOI={10.1128/AEM.70.11.6603-6610.2004}, abstractNote={ABSTRACT}, number={11}, journal={APPLIED AND ENVIRONMENTAL MICROBIOLOGY}, author={Jean, J and DH D'Souza and Jaykus, LA}, year={2004}, month={Nov}, pages={6603–6610} } @article{jean_d d'souza_jaykus_2003, title={Transcriptional enhancement of RT-PCR for rapid and sensitive detection of Noroviruses}, volume={226}, ISSN={["0378-1097"]}, DOI={10.1016/S0378-1097(03)00621-9}, abstractNote={Previously reported nucleic acid sequence-based amplification (NASBA) primers specific for the GII Noroviruses were adapted for reverse transcriptase-polymerase chain reaction (RT-PCR), and detection sensitivity was then enhanced by a subsequent in vitro transcription of the RT-PCR amplicons. The NASBA-derived primers performed comparably to other broadly reactive GII Norovirus primers with respect to detection limits (i.e. 1 RT-PCR amplifiable unit (RT-PCRU) per reaction). Detection limits improved by approximately 1 log(10) to 0.3 RT-PCRU per reaction when transcriptional enhancement and electrochemiluminescence (ECL) hybridization followed RT-PCR. The method shows promise for improved detection sensitivity in instances where very low levels of virus contamination might be anticipated.}, number={2}, journal={FEMS MICROBIOLOGY LETTERS}, author={Jean, J and D D'Souza and Jaykus, LA}, year={2003}, month={Sep}, pages={339–345} }