Denise K. Wetzel

Works (2)

Updated: July 5th, 2023 15:58

2004 journal article

Functional complementation between the PDX1 vitamin B-6 biosynthetic gene of Cercospora nicotianae and pdxJ of Escherichia coli

FEBS LETTERS, 564(1-2), 143–146.

By: D. Wetzel n, M. Ehrenshaft n, S. Denslow n & M. Daub n

author keywords: SNZ; YaaD; SOR1; PYROA; pyridoxal-5 '-phosphate; pyridoxamine
MeSH headings : Ascomycota / genetics; Cloning, Molecular; Enzymes / physiology; Escherichia coli Proteins / genetics; Escherichia coli Proteins / physiology; Fungal Proteins / genetics; Fungal Proteins / physiology; Genes, Fungal; Genetic Complementation Test; Ligases / genetics; Ligases / physiology; Oxidoreductases; Pyridoxine / biosynthesis; Transformation, Genetic; Vitamin B 6 / biosynthesis
TL;DR: The PDX1 gene of the alternative B6 pathway encodes a protein responsible for synthesis of the pyridoxine ring, which is likely to be responsible for de novo vitamin B6 biosynthesis in Escherichia coli. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Cercosporin-deficient mutants by plasmid tagging in the asexual fungus Cercospora nicotianae

MOLECULAR GENETICS AND GENOMICS, 270(2), 103–113.

By: K. Chung n, M. Ehrenshaft n, D. Wetzel n & M. Daub n

author keywords: photosensitizer; plasmid rescue; active oxygen
MeSH headings : Amino Acid Sequence; Ascomycota / genetics; Ascomycota / metabolism; Ascomycota / pathogenicity; Base Sequence; Chromosome Mapping; DNA, Fungal / genetics; Gene Targeting; Genetic Vectors; Molecular Sequence Data; Multienzyme Complexes / genetics; Multienzyme Complexes / metabolism; Mutation; Mycotoxins / biosynthesis; Mycotoxins / genetics; Perylene / analogs & derivatives; Perylene / metabolism; Phenotype; Pigmentation; Plasmids / genetics; Sequence Homology, Amino Acid; Transformation, Genetic
TL;DR: The adaptation of plasmid insertion and restriction enzyme-mediated integration to produce cercosporin toxin-deficient mutants in the asexual phytopathogenic fungus Cercospora nicotianae is successfully adapted, providing the first molecular evidence that cercOSporin is synthesized via a polyketide pathway as previously hypothesized. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

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