Denise K. Wetzel

Works (2)

Updated: July 5th, 2023 15:58

2004 journal article

Functional complementation between the PDX1 vitamin B-6 biosynthetic gene of Cercospora nicotianae and pdxJ of Escherichia coli

FEBS LETTERS, 564(1-2), 143–146.

By: D. Wetzel n, M. Ehrenshaft n, S. Denslow n & M. Daub n

author keywords: SNZ; YaaD; SOR1; PYROA; pyridoxal-5 '-phosphate; pyridoxamine
MeSH headings : Ascomycota / genetics; Cloning, Molecular; Enzymes / physiology; Escherichia coli Proteins / genetics; Escherichia coli Proteins / physiology; Fungal Proteins / genetics; Fungal Proteins / physiology; Genes, Fungal; Genetic Complementation Test; Ligases / genetics; Ligases / physiology; Oxidoreductases; Pyridoxine / biosynthesis; Transformation, Genetic; Vitamin B 6 / biosynthesis
TL;DR: The PDX1 gene of the alternative B6 pathway encodes a protein responsible for synthesis of the pyridoxine ring, which is likely to be responsible for de novo vitamin B6 biosynthesis in Escherichia coli. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2003 journal article

Cercosporin-deficient mutants by plasmid tagging in the asexual fungus Cercospora nicotianae

MOLECULAR GENETICS AND GENOMICS, 270(2), 103–113.

By: K. Chung n, M. Ehrenshaft n, D. Wetzel n & M. Daub n

author keywords: photosensitizer; plasmid rescue; active oxygen
MeSH headings : Amino Acid Sequence; Ascomycota / genetics; Ascomycota / metabolism; Ascomycota / pathogenicity; Base Sequence; Chromosome Mapping; DNA, Fungal / genetics; Gene Targeting; Genetic Vectors; Molecular Sequence Data; Multienzyme Complexes / genetics; Multienzyme Complexes / metabolism; Mutation; Mycotoxins / biosynthesis; Mycotoxins / genetics; Perylene / analogs & derivatives; Perylene / metabolism; Phenotype; Pigmentation; Plasmids / genetics; Sequence Homology, Amino Acid; Transformation, Genetic
TL;DR: The adaptation of plasmid insertion and restriction enzyme-mediated integration to produce cercosporin toxin-deficient mutants in the asexual phytopathogenic fungus Cercospora nicotianae is successfully adapted, providing the first molecular evidence that cercOSporin is synthesized via a polyketide pathway as previously hypothesized. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

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