@article{beaubois_girard_lallechere_davies_paladian_bonnet_ledoigt_vian_2007, title={Intercellular communication in plants: Evidence for two rapidly transmitted systemic signals generated in response to electromagnetic field stimulation in tomato}, volume={30}, ISSN={["1365-3040"]}, DOI={10.1111/j.1365-3040.2007.01669.x}, abstractNote={ABSTRACT}, number={7}, journal={PLANT CELL AND ENVIRONMENT}, author={Beaubois, Elisabeth and Girard, Sebastien and Lallechere, Sebastien and Davies, Eric and Paladian, Francoise and Bonnet, Pierre and Ledoigt, Gerard and Vian, Alain}, year={2007}, month={Jul}, pages={834–844} }
 @article{roux_vian_girard_bonnet_paladian_davies_ledoigt_2006, title={Electromagnetic fields (900 MHz) evoke consistent molecular responses in tomato plants}, volume={128}, ISSN={["1399-3054"]}, DOI={10.1111/j.1399-3054.2006.00740.x}, abstractNote={Although the effects of high-frequency electromagnetic fields on biological systems have been studied frequently, unequivocal results have rarely been obtained, primarily because suitably controlled experiments could not be performed. In the present work, tomato plants were exposed to a homogeneous and isotropic field (900 MHz) using a mode stirred reverberation chamber, and the stress-related transcripts (calmodulin, protease inhibitor and chloroplast mRNA-binding protein) were assayed by real-time quantitative PCR. Exposure to an electromagnetic field induced a biphasic response, in which the levels of all three transcripts increased four-to six-fold 15 min after the end of electromagnetic stimulation, dropped to close to initial levels by 30 min, and then increased again at 60 min. We deliberately focused on the very early molecular responses to high-frequency electromagnetic fields in order to minimize secondary effects.}, number={2}, journal={PHYSIOLOGIA PLANTARUM}, author={Roux, David and Vian, Alain and Girard, Sebastien and Bonnet, Pierre and Paladian, Francoise and Davies, Eric and Ledoigt, Gerard}, year={2006}, month={Oct}, pages={283–288} }
 @article{stankovic_vian_henry-vian_davies_2000, title={Molecular cloning and characterization of a tomato cDNA encoding a systemically wound-inducible bZIP DNA-binding protein}, volume={212}, ISSN={["0032-0935"]}, DOI={10.1007/s004250000362}, abstractNote={Localized wounding of one leaf in intact tomato (Lycopersicon esculentum Mill.) plants triggers rapid systemic transcriptional responses that might be involved in defense. To better understand the mechanism(s) of intercellular signal transmission in wounded tomatoes, and to identify the array of genes systemically up-regulated by wounding, a subtractive cDNA library for wounded tomato leaves was constructed. A novel cDNA clone (designated LebZIP1) encoding a DNA-binding protein was isolated and identified. This clone appears to be encoded by a single gene, and belongs to the family of basic leucine zipper domain (bZIP) transcription factors shown to be up-regulated by cold and dark treatments. Analysis of the mRNA levels suggests that the transcript for LebZIP1 is both organ-specific and up-regulated by wounding. In wounded wild-type tomatoes, the LebZIP1 mRNA levels in distant tissue were maximally up-regulated within only 5 min following localized wounding. Exogenous abscisic acid (ABA) prevented the rapid wound-induced increase in LebZIP1 mRNA levels, while the basal levels of LebZIP1 transcripts were higher in the ABA mutants notabilis (not), sitiens (sit), and flacca (flc), and wound-induced increases were greater in the ABA-deficient mutants. Together, these results suggest that ABA acts to curtail the wound-induced synthesis of LebZIP1 mRNA.}, number={1}, journal={PLANTA}, author={Stankovic, B and Vian, A and Henry-Vian, C and Davies, E}, year={2000}, month={Dec}, pages={60–66} }
 @article{vian_henry-vian_davies_1999, title={Rapid and systemic accumulation of chloroplast mRNA-binding protein transcripts after flame stimulus in tomato}, volume={121}, ISSN={["0032-0889"]}, DOI={10.1104/pp.121.2.517}, abstractNote={Abstract}, number={2}, journal={PLANT PHYSIOLOGY}, author={Vian, A and Henry-Vian, C and Davies, E}, year={1999}, month={Oct}, pages={517–524} }
 @article{vian_henryvian_schantz_schantz_davies_ledoigt_desbiez_1997, title={Effect of calcium and calcium-counteracting drugs on the response of Bidens pilosa L to wounding}, volume={38}, ISSN={["1471-9053"]}, DOI={10.1093/oxfordjournals.pcp.a029231}, abstractNote={We used calcium counteracting drugs known to reduce the amplitude of wound-induced electric wave of depolarization and we showed that in these conditions, accumulation of the calmodulin mRNA (recently found to be correlated to membrane potential) is strongly reduced. These results bring additional evidence linking membrane potential and calmodulin mRNA accumulation.}, number={6}, journal={PLANT AND CELL PHYSIOLOGY}, author={Vian, A and HenryVian, C and Schantz, R and Schantz, ML and Davies, E and Ledoigt, G and Desbiez, MO}, year={1997}, month={Jun}, pages={751–753} }
 @article{davies_vian_vian_stankovic_1997, title={Rapid systemic up-regulation of genes after heat-wounding and electrical stimulation}, volume={19}, ISSN={["0137-5881"]}, DOI={10.1007/s11738-997-0055-0}, abstractNote={When one leaf of a tomato plant is electrically-stimulated or heat-wounded, proteinase inhibitor genes are rapidly up-regulated in distant leaves. The identity of the systemic wound signal(s) is not yet known, but major candidates include hormones transmitted via the phloem or the xylem, the electrically-stimulated self-propagating electrical signal in the phloem (the action potential, AP), or the heat-wound-induced surge in hydraulic pressure in the xylem evoking a local change in membrane potential in adjacent living cells (the variation potential, VP). In order to discriminate between these signals we have adopted two approaches. The first approach involves applying stimuli that evoke known signals and determining whether these signals have similar effects on the "model" transcripts for proteinase inhibitors (pin) and calmodulin (cal). Here we show that a heat wound almost invariably evokes a VP, while an electrical stimulation occasionally evokes an AP, and both of these signals induce accumulation of transcripts encoding proteinase inhibitors. The second approach involves identifying the array of genes turned on by heat-wounding. To this end, we have constructed a subtractive library for heat-wounded tissue, isolated over 800 putatively up-regulated clones, and shown that all but two of the fifty that we have analyzed by Northern hybridization are, indeed, up-regulated. Here we show the early kinetics of up-regulation of three of these transcripts in the terminal (4th) leaf in response to heat-wounding the 3rd leaf, about 5 cm away. Even though these transcripts show somewhat different time courses of induction, with one peaking at 30 min, another at 15 min, and another at 5 min after flaming of a distant leaf, they all exhibit a similar pattern, i.e., a transient period of transcript accumulation preceding a period of transcript decrease, followed by a second period of transcript accumulation.}, number={4}, journal={ACTA PHYSIOLOGIAE PLANTARUM}, author={Davies, E and Vian, A and Vian, C and Stankovic, B}, year={1997}, pages={571–576} }