@article{craven_velez_cho_lawrence_mitchell_2008, title={Anastomosis is required for virulence of the fungal necrotroph Alternaria brassicicola}, volume={7}, ISSN={["1535-9786"]}, DOI={10.1128/EC.00423-07}, abstractNote={ABSTRACT A fungal mycelium is typically composed of radially extending hyphal filaments interconnected by bridges created through anastomoses. These bridges facilitate the dissemination of nutrients, water, and signaling molecules throughout the colony. In this study, we used targeted gene deletion and nitrate utilization mutants of the cruciferous pathogen Alternaria brassicicola and two closely related species to investigate hyphal fusion (anastomosis) and its role in the ability of fungi to cause disease. All eight of the A. brassicicola isolates tested, as well as A. mimicula and A. japonica, were capable of self-fusion, with two isolates of A. brassicicola being capable of non-self-fusion. Disruption of the anastomosis gene homolog (Aso1) in A. brassicicola resulted in both the loss of self-anastomosis and pathogenicity on cabbage. This finding, combined with our discovery that a previously described nonpathogenic A. brassicicola mutant defective for a mitogen-activated protein kinase gene (amk1) also lacked the capacity for self-anastomosis, suggests that self-anastomosis is associated with pathogenicity in A. brassicicola.}, number={4}, journal={EUKARYOTIC CELL}, author={Craven, Kelly D. and Velez, Heriberto and Cho, Yangrae and Lawrence, Christopher B. and Mitchell, Thomas K.}, year={2008}, month={Apr}, pages={675–683} }
 @article{cramer_la rota_cho_thon_craven_knudson_mitchell_lawrence_2006, title={Bioinformatic analysis of expressed sequence tags derived from a compatible Alternaria brassicicola-Brassica oleracea interaction}, volume={7}, ISSN={["1364-3703"]}, DOI={10.1111/J.1364-3703.2006.00324.X}, abstractNote={SUMMARY Alternaria brassicicola is a necrotrophic fungal pathogen that causes black spot disease on members of the Brassicaceae plant family. In order to identify candidate fungal pathogenicity genes and characterize a compatible host response, a suppression subtractive hybridization (SSH) cDNA library enriched for A. brassicicola and Brassica oleracea genes expressed during the interaction was created, along with a fungal cDNA library representing genes expressed during nitrogen starvation (NS). A total of 3749 and 2352 expressed sequence tags (ESTs) were assembled into 2834 and 1264 unisequence sets for the SSH and NS libraries, respectively. We compared two methods to identify the origins (plant vs. fungal) of ESTs in the SSH library using different classification procedures, with and without the availability of a database representing the A. brassicicola whole genome sequence and Brassicaceae-specific genes. BLASTX analyses of the 2834 unisequence set using the GenBank non-redundant database identified 114 fungal genes. Further BLASTN analyses of the genes with unidentifiable origin using a database consisting of the 1264 fungal unisequence set from the nitrogen-starved library identified 94 additional fungal genes. By contrast, BLASTN analyses of the same SSH unisequence set using a partially assembled A. brassicicola whole genome draft sequence identified a total of 310 unisequenes of fungal origin. Our results indicated that even a small number of organism-specific EST sequences can be very helpful to identify pathogen genes in a library derived from infected tissue, partially overcoming the limitation of the public databases for little studied organisms. However, using the whole genome draft sequence of A. brassicicola we were able to identify approximately 30% more fungal genes in the SSH library than without utilizing this resource. The putative role of specific fungal and plant genes identified in this study in a compatible interaction is discussed.}, number={2}, journal={MOLECULAR PLANT PATHOLOGY}, author={Cramer, RA and La Rota, CM and Cho, Y and Thon, M and Craven, KD and Knudson, DL and Mitchell, TK and Lawrence, CB}, year={2006}, month={Mar}, pages={113–124} }
 @article{craven_peterson_windham_mitchell_martin_2005, title={Molecular identification of the turf grass rapid blight pathogen}, volume={97}, ISSN={["1557-2536"]}, DOI={10.3852/mycologia.97.1.160}, abstractNote={Rapid blight is a newly described disease on turf grasses, primarily found on golf courses using suboptimal water for irrigation purposes. On the basis of shared morphological characteristics, it has been proposed that the rapid blight pathogen belongs to a genus of stramenopiles, Labyrinthula, which had been known to cause disease of marine plants only. We have collected 10 isolates from four species of turf grass in five states and sequenced portions of the SSU (18S) rDNA gene from each to provide a definitive taxonomic placement for rapid blight pathogens. We also included sequences from Labyrinthuloides yorkensis, Schizochytrium aggregatum, Aplanochytrium sp., Thraustochytrium striatum, Achlya bisexualis and several nonturf-grass isolates of Labyrinthula. We found that rapid blight isolates indeed are placed firmly within the genus Labyrinthula and that they lack detectable genetic diversity in the 18S rDNA region. We propose that the rapid blight pathogens share a recent common ancestor and might have originated from a single, infected population.}, number={1}, journal={MYCOLOGIA}, author={Craven, KD and Peterson, PD and Windham, DE and Mitchell, TK and Martin, SB}, year={2005}, pages={160–166} }
 @article{moon_craven_leuchtmann_clement_schardl_2004, title={Prevalence of interspecific hybrids amongst asexual fungal endophytes of grasses}, volume={13}, ISSN={["1365-294X"]}, DOI={10.1111/j.1365-294X.2004.02138.x}, abstractNote={Abstract}, number={6}, journal={MOLECULAR ECOLOGY}, author={Moon, CD and Craven, KD and Leuchtmann, A and Clement, SL and Schardl, CL}, year={2004}, month={Jun}, pages={1455–1467} }