@article{skalski_couch_garber_weir_sullivan_2006, title={Evaluation of DNA pooling for the estimation of microsatellite allele frequencies: A case study using striped bass (Morone saxatilis)}, volume={173}, ISSN={["1943-2631"]}, DOI={10.1534/genetics.105.053702}, abstractNote={Using striped bass (Morone saxatilis) and six multiplexed microsatellite markers, we evaluated procedures for estimating allele frequencies by pooling DNA from multiple individuals, a method suggested as cost-effective relative to individual genotyping. Using moment-based estimators, we estimated allele frequencies in experimental DNA pools and found that the three primary laboratory steps, DNA quantitation and pooling, PCR amplification, and electrophoresis, accounted for 23, 48, and 29%, respectively, of the technical variance of estimates in pools containing DNA from 2–24 individuals. Exact allele-frequency estimates could be made for pools of sizes 2–8, depending on the locus, by using an integer-valued estimator. Larger pools of size 12 and 24 tended to yield biased estimates; however, replicates of these estimates detected allele frequency differences among pools with different allelic compositions. We also derive an unbiased estimator of Hardy–Weinberg disequilibrium coefficients that uses multiple DNA pools and analyze the cost-efficiency of DNA pooling. DNA pooling yields the most potential cost savings when a large number of loci are employed using a large number of individuals, a situation becoming increasingly common as microsatellite loci are developed in increasing numbers of taxa.}, number={2}, journal={GENETICS}, author={Skalski, Garrick T. and Couch, Charlene R. and Garber, Amber F. and Weir, Bruce S. and Sullivan, Craig V.}, year={2006}, month={Jun}, pages={863–875} } @article{rexroad_vallejo_coulibaly_couch_garber_westerman_sullivan_2006, title={Identification and characterization of microsatellites for striped bass from repeat-enriched libraries}, volume={7}, ISSN={["1572-9737"]}, DOI={10.1007/s10592-006-9122-0}, number={6}, journal={CONSERVATION GENETICS}, author={Rexroad, Caird and Vallejo, Roger and Coulibaly, Issa and Couch, Charlene and Garber, Amber and Westerman, Mark and Sullivan, Craig}, year={2006}, month={Dec}, pages={971–982} } @article{couch_garber_rexroad_abrams_stannard_westerman_sullivan_2006, title={Isolation and characterization of 149 novel microsatellite DNA markers for striped bass, Morone saxatilis, and cross-species amplification in white bass, Morone chrysops, and their hybrid}, volume={6}, ISSN={["1471-8278"]}, DOI={10.1111/j.1471-8286.2006.01292.x}, abstractNote={To support detailed genetic analysis of striped bass ( Morone saxatilis ) and white bass ( Morone chrysops ), we isolated 153 microsatellite loci from repeat-enriched striped bass DNA libraries. Of these, 147 markers amplified in striped bass (average 4.7 alleles per locus) and 133 in white bass (average 2.2 alleles per locus). One hundred twenty-two markers amplified in their hybrid. Development of new microsatellite markers will facilitate evaluations of genetic structure in wild populations and will support pedigree analysis and linkage mapping for selective breeding.}, number={3}, journal={MOLECULAR ECOLOGY NOTES}, author={Couch, C. R. and Garber, A. . F. and Rexroad, C. E., III and Abrams, J. . M. and Stannard, J. . A. and Westerman, M. E. and Sullivan, C. V.}, year={2006}, month={Sep}, pages={667–669} } @misc{garber_sullivan_2006, title={Selective breeding for the hybrid striped bass (Morone chrysops, Rafinesque x M-saxatilis, Walbaum) industry: status and perspectives}, volume={37}, ISSN={["1365-2109"]}, DOI={10.1111/j.1365-2109.2005.01439.x}, abstractNote={The hybrid striped bass (HSB) farming industry has considerable potential for growth into domestic retail markets, but expansion of this industry is limited by high production costs that dictate high prices for HSB. It is widely recognized within the industry that selective breeding of an improved HSB will be required to increase production efficiency and reduce market prices. A National Program of Genetic Improvement and Selective Breeding for the HSB Industry has been initiated and some progress has been made toward domestication of the parent species of the hybrid. However, uncertainty remains as to which breeding procedures will most rapidly yield sustainable genetic gains in key production traits. This paper consolidates and reviews general information on the biology of temperate basses (genus Morone) relevant to selective breeding of improved HSB. The topics covered include control of reproduction, geographic distribution of stocks and population genetic variation. This is followed by a brief review of the current application of selective breeding techniques, including those based on molecular markers. Finally, we discuss potential avenues for genetic improvement of HSB in a selective breeding program.}, number={4}, journal={AQUACULTURE RESEARCH}, author={Garber, AF and Sullivan, CV}, year={2006}, month={Mar}, pages={319–338} } @article{garber_tringali_franks_2005, title={Population genetic and phylogeographic structure of wahoo, Acanthocybium solandri, from the western central Atlantic and central Pacific Oceans}, volume={147}, number={1}, journal={Marine Biology (Berlin, Germany)}, author={Garber, A. F. and Tringali, M. D. and Franks, J. S.}, year={2005}, pages={205–214} } @article{garber_tringali_stuck_2004, title={Population structure and variation in red snapper (Lutjanus campechanus) from the Gulf of Mexico and Atlantic coast of Florida as determined from mitochondrial DNA control region sequence}, volume={6}, number={2}, journal={Marine Biotechnology (New York, N.Y.)}, author={Garber, A. F. and Tringali, M. D. and Stuck, K. C.}, year={2004}, pages={175–185} }