@article{smith_berger_freeman_2006, title={High affinity, high efficiency fibre-reactive dyes}, volume={122}, ISSN={["1472-3581"]}, DOI={10.1111/j.1478-4408.2006.00032.x}, abstractNote={A straightforward two‐step method for modifying commercial dichlorotriazine‐based fibre‐reactive dyes prior to their use in the dyeing process can greatly improve affinity and fixation efficiency on cotton, and reduce the salt requirements. The modification used in this study involved prereacting the commercial dyes with either cysteine or cysteamine followed by reaction of the resulting intermediate with cyanuric chloride. Cotton fabric dyed with the modified dyes had technical properties that were essentially equal to those obtained from the unmodified commercial dyes.}, number={4}, journal={COLORATION TECHNOLOGY}, author={Smith, Brent and Berger, Rebecca and Freeman, Harold S.}, year={2006}, pages={187–193} } @article{gibson_riley-berger_harshman_kopp_vacha_nuzhdin_wayne_2004, title={Extensive sex-specific nonadditivity of gene expression in Drosophila melanogaster}, volume={167}, ISSN={["1943-2631"]}, DOI={10.1534/genetics.104.026583}, abstractNote={Abstract Assessment of the degree to which gene expression is additive and heritable has important implications for understanding the maintenance of variation, adaptation, phenotypic divergence, and the mapping of genotype onto phenotype. We used whole-genome transcript profiling using Agilent long-oligonucleotide microarrays representing 12,017 genes to demonstrate that gene transcription is pervasively nonadditive in Drosophila melanogaster. Comparison of adults of two isogenic lines and their reciprocal F1 hybrids revealed 5820 genes as significantly different between at least two of the four genotypes in either males or females or across both sexes. Strikingly, while 25% of all genes differ between the two parents, 33% differ between both F1's and the parents, averaged across sexes. However, only 5% of genes show overdominance, suggesting that heterosis for expression is rare.}, number={4}, journal={GENETICS}, author={Gibson, G and Riley-Berger, R and Harshman, L and Kopp, A and Vacha, S and Nuzhdin, S and Wayne, M}, year={2004}, month={Aug}, pages={1791–1799} } @article{palsson_rouse_riley-berger_dworkin_gibson_2004, title={Nucleotide variation in the Egfr locus of Drosophila melanogaster}, volume={167}, ISSN={["1943-2631"]}, DOI={10.1534/genetics.104.026252}, abstractNote={Abstract The Epidermal growth factor receptor is an essential gene with diverse pleiotropic roles in development throughout the animal kingdom. Analysis of sequence diversity in 10.9 kb covering the complete coding region and 6.4 kb of potential regulatory regions in a sample of 250 alleles from three populations of Drosophila melanogaster suggests that the intensity of different population genetic forces varies along the locus. A total of 238 independent common SNPs and 20 indel polymorphisms were detected, with just six common replacements affecting >1475 amino acids, four of which are in the short alternate first exon. Sequence diversity is lowest in a 2-kb portion of intron 2, which is also highly conserved in comparison with D. simulans and D. pseudoobscura. Linkage disequilibrium decays to background levels within 500 bp of most sites, so haplotypes are generally restricted to up to 5 polymorphisms. The two North American samples from North Carolina and California have diverged in allele frequency at a handful of individual SNPs, but a Kenyan sample is both more divergent and more polymorphic. The effect of sample size on inference of the roles of population structure, uneven recombination, and weak selection in patterning nucleotide variation in the locus is discussed.}, number={3}, journal={GENETICS}, author={Palsson, A and Rouse, A and Riley-Berger, R and Dworkin, I and Gibson, G}, year={2004}, month={Jul}, pages={1199–1212} }