@article{patel_hamamouch_li_hewezi_hussey_baum_mitchum_davis_2010, title={A nematode effector protein similar to annexins in host plants}, volume={61}, ISSN={["1460-2431"]}, DOI={10.1093/jxb/erp293}, abstractNote={Nematode parasitism genes encode secreted effector proteins that play a role in host infection. A homologue of the expressed Hg4F01 gene of the root-parasitic soybean cyst nematode, Heterodera glycines, encoding an annexin-like effector, was isolated in the related Heterodera schachtii to facilitate use of Arabidopsis thaliana as a model host. Hs4F01 and its protein product were exclusively expressed within the dorsal oesophageal gland secretory cell in the parasitic stages of H. schachtii. Hs4F01 had a 41% predicted amino acid sequence identity to the nex-1 annexin of C. elegans and 33% identity to annexin-1 (annAt1) of Arabidopsis, it contained four conserved domains typical of the annexin family of calcium and phospholipid binding proteins, and it had a predicted signal peptide for secretion that was present in nematode annexins of only Heterodera spp. Constitutive expression of Hs4F01 in wild-type Arabidopsis promoted hyper-susceptibility to H. schachtii infection. Complementation of an AnnAt1 mutant by constitutive expression of Hs4F01 reverted mutant sensitivity to 75mM NaCl, suggesting a similar function of the Hs4F01 annexin-like effector in the stress response by plant cells. Yeast two-hybrid assays confirmed a specific interaction between Hs4F01 and an Arabidopsis oxidoreductase member of the 2OG-Fe(II) oxygenase family, a type of plant enzyme demonstrated to promote susceptibility to oomycete pathogens. RNA interference assays that expressed double-stranded RNA complementary to Hs4F01 in transgenic Arabidopsis specifically decreased parasitic nematode Hs4F01 transcript levels and significantly reduced nematode infection levels. The combined data suggest that nematode secretion of an Hs4F01 annexin-like effector into host root cells may mimic plant annexin function during the parasitic interaction.}, number={1}, journal={JOURNAL OF EXPERIMENTAL BOTANY}, author={Patel, Nrupali and Hamamouch, Noureddine and Li, Chunying and Hewezi, Tarek and Hussey, Richard S. and Baum, Thomas J. and Mitchum, Melissa G. and Davis, Eric L.}, year={2010}, month={Jan}, pages={235–248} }
 @article{patel_hamamouch_li_hussey_mitchum_baum_wang_davis_2008, title={Similarity and functional analyses of expressed parasitism genes in Heterodera schachtii and Heterodera glycines}, volume={40}, number={4}, journal={Journal of Nematology}, author={Patel, N. and Hamamouch, N. and Li, C. Y. and Hussey, R. and Mitchum, M. and Baum, T. and Wang, X. H. and Davis, E. L.}, year={2008}, pages={299–310} }
 @article{patel_cardoza_christensen_rekapalli_ayalew_stewart_2004, title={Differential gene expression of Chlamydomonas reinhardtii in response to 2,4,6-trinitrotoluene (TNT) using microarray analysis}, volume={167}, ISSN={["0168-9452"]}, DOI={10.1016/j.plantsci.2004.06.013}, abstractNote={The exposure of Chlamydomonas reinhardtii to environmental stress, such as that caused by the explosive 2,4,6-trinitrotoluene (TNT) can alter its gene expression. Expression analysis was conducted using a microarray composed of 3079 Chlamydomonas ESTs to characterize the broad range of responses of gene expression exposed to this common ordnance compound. TNT treatment conditions were determined by growth analysis of Chlamydomonas in 0–5 μg/mL TNT. One and 3 μg/mL were used for microarray analysis since 1 μg/mL of TNT did not decrease the cell count after 7 days of treatment, whereas 3 μg/mL of TNT was the maximum TNT concentration that allowed growth, respectively. Transcriptional profiling revealed that approximately 158 responsive genes were differentially expressed representing several functional categories. Genes responsible for photosynthesis, energy metabolism and oxidative stress were upregulated in the presence of TNT, while the expression of cell wall related genes were downregulated. Several unidentified genes were also affected. The microarray results were validated using real-time RT-PCR for a subset of genes. Information from the microarray analysis can be used to engineer algae-based sensors to signal TNT exposure in addition to potential explosives cleanup applications.}, number={5}, journal={PLANT SCIENCE}, author={Patel, N and Cardoza, V and Christensen, E and Rekapalli, L and Ayalew, M and Stewart, CN}, year={2004}, month={Nov}, pages={1109–1122} }