@article{graves_liwimbi_israel_heugten_robinson_cahoon_lubbers_2011, title={Distribution of ten antibiotic resistance genes in E. coli isolates from swine manure, lagoon effluent and soil collected from a lagoon waste application field}, volume={56}, ISSN={["0015-5632"]}, DOI={10.1007/s12223-011-0019-z}, abstractNote={The prevalence of ten antibiotic resistance genes (ARGs) was evaluated in a total of 616 Escherichia coli isolates from swine manure, swine lagoon effluent, and from soils that received lagoon effluent on a commercial swine farm site in Sampson County, North Carolina (USA). Isolates with ARGs coding for streptomycin/spectinomycin (aadA/strA and strB), tetracycline (tetA and tetB), and sulfonamide (sul1) occurred most frequently (60.6-91.3%). The occurrence of E. coli isolates that carried aadA, tetA, tetB, and tetC genes was significantly more frequent in soil samples (34.0-97.2%) than in isolates from lagoon samples (20.9-90.6%). Furthermore, the frequency of isolates that contain genes coding for aadA and tetB was significantly greater in soil samples (82.6-97.2%) when compared to swine manure (16.8-86.1%). Isolates from the lagoon that carried tetA, tetC, and sul3 genes were significantly more prevalent during spring (63.3-96.7%) than during winter (13.1-67.8%). The prevalence of isolates from the lagoon that possessed the strA, strB, and sul1 resistance genes was significantly more frequent during the summer (90.0-100%) than during spring (66.6-80.0%). The data suggest that conditions in the lagoon, soil, and manure may have an impact on the occurrence of E. coli isolates with specific ARGs. Seasonal variables seem to impact the recovery isolates with ARGs; however, ARG distribution may be associated with mobile genetic elements or a reflection of the initial numbers of resistant isolates shed by the animals.}, number={2}, journal={FOLIA MICROBIOLOGICA}, author={Graves, A. K. and Liwimbi, L. and Israel, D. W. and Heugten, E. and Robinson, B. and Cahoon, C. W. and Lubbers, J. F.}, year={2011}, month={Mar}, pages={131–137} }
 @article{robinson_moffitt_wilkerson_jordan_2007, title={Economics and effectiveness of alternative weed scouting methods in peanut}, volume={21}, ISSN={["1550-2740"]}, DOI={10.1614/WT-05-190.1}, abstractNote={On-farm trials were conducted in 16 North Carolina peanut fields to obtain estimates of scouting times and quality of herbicide recommendations for different weed scouting methods. The fields were monitored for weed species and population density using four scouting methods: windshield (estimate made from the edge of the field), whole-field (estimate based on walk through the field), range (weed densities rated on 1–5 scale at six locations in the field), and counts (weeds estimated by counting at six locations in the field). The herbicide application decision support system (HADSS) was used to determine theoretical net return over herbicide investment and yield loss ($ and %) for each treatment in each field. Three scouts estimated average weed population densities using each scouting method. These values were entered into HADSS to obtain treatment recommendations. Independently collected count data from all three scouts were combined to determine the optimal treatment in each field and the relative ranking of each available treatment. When using the whole-field method, scouts observed a greater number of weed species than when using the other methods. The windshield, whole-field, and range scouting methods tended to overestimate density slightly at low densities and underestimate density substantially at high densities, compared to the count method. The windshield method required the least amount of time to complete (6 min per field), but also resulted in the greatest average loss. Even for this method, recommendations had theoretical net returns within 10% of the return for the optimal treatment 80% of the time. The count method appears to have less economic risk than the windshield, whole-field, and range scouting methods.}, number={1}, journal={WEED TECHNOLOGY}, author={Robinson, Bridget L. and Moffitt, Jodie M. and Wilkerson, Gail G. and Jordan, David L.}, year={2007}, pages={88–96} }
 @article{thomas_burke_robinson_pline-srnic_edmisten_wells_wilcut_2005, title={Yield and physiological response of nontransgenic cotton to simulated glyphosate drift}, volume={19}, ISSN={["1550-2740"]}, DOI={10.1614/WT-03-194R}, abstractNote={Field studies were conducted in 2001 in Lewiston, NC, and in 2002 at Clayton and Lewiston, NC, to investigate the response of nontransgenic cotton to simulated glyphosate drift in a weed-free environment. Nontransgenic cotton variety ‘Fibermax 989’ was planted in a conventional seedbed at all locations. Glyphosate treatments were applied early postemergence (EPOST) at the four-leaf growth stage of cotton at 0, 8.7, 17.5, 35, 70, 140, 280, 560, and 1,120 g ai/ha and represent 0, 0.78, 1.55, 3.13, 6.25, 12.5, 25, 50, and 100% of the commercial use rate, respectively. Rates as low as 140 g/ha caused lint yield reductions depending on year and location. When averaged over all locations, lint yield reductions of 4, 49, 72, and 87% compared with nontreated cotton were observed with glyphosate rates of 140, 280, 560, and 1,120 g/ha, respectively. Visual injury and shikimic acid accumulation were evident at glyphosate rates greater or equal to 70 g/ha. Collectively, visual injury and shikimic acid accumulation at 7 d after EPOST treatment might be used as a diagnostic indicator to predict potential yield reductions from simulated glyphosate drift. Nomenclature: Glyphosate; cotton, Gossypium hirsutum L. ‘Fibermax 989’. Additional index words: Shikimic acid. Abbreviations: DAT, days after early postemergence treatment; DD, degree-day; EPOST, early postemergence; EPSPS, 5-enolpyruvylshikimate-3-phosphate synthase [EC 2.5.1.19]; HPLC, high-performance liquid chromatography; PDS, postemergence-directed; POST, postemergence; PRE, preemergence.}, number={1}, journal={WEED TECHNOLOGY}, author={Thomas, WE and Burke, IC and Robinson, BL and Pline-Srnic, WA and Edmisten, KL and Wells, R and Wilcut, JW}, year={2005}, pages={35–42} }
 @article{wilkerson_price_bennett_krueger_roberson_robinson_2004, title={Evaluating the potential for site-specific herbicide application in soybean}, volume={18}, ISSN={["0890-037X"]}, DOI={10.1614/WT-03-258R}, abstractNote={Field experiments were conducted on two North Carolina research stations in 1999, 2000, and 2001; on-farm in Lenoir, Wayne, and Wilson counties, NC, in 2002; and on-farm in Port Royal, VA, in 2000, 2001, and 2002 to evaluate possible gains from site-specific herbicide applications at these locations. Fields were scouted for weed populations using custom software on a handheld computer linked to a Global Positioning System. Scouts generated field-specific sampling grids and recorded weed density information for each grid cell. The decision aid HADSS™ (Herbicide Application Decision Support System) was used to estimate expected net return and yield loss remaining after treatment in each sample grid of every field under differing assumptions of weed size and soil moisture conditions, assuming the field was planted with either conventional or glyphosate-resistant (GR) soybean. The optimal whole-field treatment (that treatment with the highest expected net return summed across all grid cells within a field) resulted in average theoretical net returns of $79/ha (U.S. dollars) and $139/ha for conventional and GR soybean, respectively. When the most economical treatment for each grid cell was used in site-specific weed management, theoretical net returns increased by $13/ha (conventional) and $4.50/ha (GR), and expected yield loss after treatment was reduced by 10.5 and 4%, respectively, compared with the whole-field optimal treatment. When the most effective treatment for each grid cell was used in site-specific weed management, theoretical net returns decreased by $18/ha (conventional) and $4/ha (GR), and expected yield loss after treatment was reduced by 27 and 19%, respectively, compared with the whole-field optimal treatment. Site-specific herbicide applications could have reduced the volume of herbicides sprayed by as much as 70% in some situations but increased herbicide amounts in others. On average, the whole-field treatment was optimal in terms of net return for only 35% (conventional) and 57% (GR) of grid cells.}, number={4}, journal={WEED TECHNOLOGY}, author={Wilkerson, GG and Price, AJ and Bennett, AC and Krueger, DW and Roberson, GT and Robinson, BL}, year={2004}, pages={1101–1110} }