Nicole Donofrio

Works (7)

Updated: July 5th, 2023 15:57

2018 article

Characterizing Small RNAs in Filamentous Fungi Using the Rice Blast Fungus, Magnaporthe oryzae, as an Example

PLANT PATHOGENIC FUNGI AND OOMYCETES: METHODS AND PROTOCOLS, Vol. 1848, pp. 53–66.

By: V. Raman *, B. Meyers *, R. Dean n  & N. Donofrio*

author keywords: sRNAs; Small interfering RNAs; CPA-sRNAs; RNAseq; Fungus
MeSH headings : Computational Biology / methods; Gene Library; Magnaporthe / genetics; Oryza / microbiology; Plant Diseases / microbiology; RNA, Fungal; RNA, Small Untranslated; Software; User-Computer Interface
TL;DR: Protocols for comparative analysis of sRNAs in different conditions, comparisons of sRNA libraries to RNAseq data and identification and analysis of methylguanosine-capped and polyadenylated s RNAs (CPA-sRNAs) are presented. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: May 13, 2019

2008 review

Transcriptome analysis reveals new insight into appressorium formation and function in the rice blast fungus Magnaporthe oryzae

[Review of ]. GENOME BIOLOGY, 9(5).

By: Y. Oh n, N. Donofrio n, H. Pan n, S. Coughlan*, D. Brown n, S. Meng n, T. Mitchell n, R. Dean n 

MeSH headings : Cell Wall / chemistry; Cell Wall / metabolism; Fungal Proteins / genetics; Fungal Proteins / metabolism; Gene Expression Profiling; Gene Expression Regulation, Fungal; Lipid Metabolism; Magnaporthe / cytology; Magnaporthe / genetics; Magnaporthe / physiology; Signal Transduction; Spores, Fungal / cytology; Spores, Fungal / genetics
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 journal article

A systematic analysis of T-DNA insertion events in Magnaporthe oryzae

FUNGAL GENETICS AND BIOLOGY, 44(10), 1050–1064.

By: Y. Meng *, G. Patel *, M. Heist*, M. Betts*, S. Tucker*, N. Galadima*, N. Donofrio n, D. Brown n ...

author keywords: agrobacterium-mediated transformation; insertional mutagenesis; inverse PCR
MeSH headings : 3' Flanking Region; 5' Flanking Region; Agrobacterium tumefaciens / genetics; Chromosomes, Fungal; DNA, Bacterial / analysis; DNA, Bacterial / genetics; Genome, Fungal; Magnaporthe / genetics; Mutagenesis, Insertional; Repetitive Sequences, Nucleic Acid; Transformation, Genetic
TL;DR: Analysis of random T-DNA insertions that were generated as part of a large-scale insertional mutagenesis project for Magnaporthe oryzae found that the distribution of T- DNA inserts among the M.Oryzae chromosomes was not random and significant insertion bias was detected. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 journal article

Development of a high throughput transformation system for insertional mutagenesis in Magnaporthe oryzae

FUNGAL GENETICS AND BIOLOGY, 44(10), 1035–1049.

By: M. Betts, S. Tucker*, N. Galadima*, Y. Meng *, G. Patel *, L. Li *, N. Donofrio n, A. Floyd n ...

author keywords: Magnaporthe oryzae; Insertional mutagenesis; Agrobacterium tumefaciens-mediated transformation; CaCl2/PEG-mediated transformation; pathogenicity
MeSH headings : 3' Flanking Region; 5' Flanking Region; Agrobacterium tumefaciens / genetics; Magnaporthe / genetics; Mutagenesis, Insertional / methods; Phenotype; Protoplasts; Transformation, Genetic
TL;DR: A description of the high throughput transformation and analysis pipeline used to create the most comprehensive insertional mutagenesis analysis of a fungal pathogen is provided. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2006 journal article

Global gene expression during nitrogen starvation in the rice blast fungus, Magnaporthe grisea

FUNGAL GENETICS AND BIOLOGY, 43(9), 605–617.

By: N. Donofrio n, Y. Oh, R. Lundy, H. Pan*, D. Brown*, J. Jeong*, S. Coughlan, T. Mitchell*, R. Dean* 

author keywords: nitrogen limitation; GATA transcription factors; metabolism; SPM1; protease; targeted gene disruption; pathogenicity
MeSH headings : Amino Acids / metabolism; Binding Sites; Biological Transport / genetics; DNA-Binding Proteins / genetics; GATA Transcription Factors / metabolism; Gene Expression; Gene Expression Regulation, Fungal; Genes, Fungal; Magnaporthe / genetics; Magnaporthe / pathogenicity; Nitrogen / metabolism; Oligonucleotide Array Sequence Analysis; Oryza / metabolism; Oryza / microbiology; Plant Diseases / microbiology; Promoter Regions, Genetic; Serine Endopeptidases / genetics
TL;DR: A genome-wide analysis of gene expression under nitrogen-limiting conditions in the rice blast pathogen Magnaporthe grisea resulted in targeted gene replacement on the vacuolar serine protease, SPM1, resulting in decreased sporulation and appressorial development as well as a greatly attenuated ability to cause disease. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2005 journal article

PACLIMS: A component LIM system for high-throughput functional genomic analysis

BMC Bioinformatics, 6.

By: N. Donofrio, R. Rajagopalon, D. Brown, S. Diener, D. Windham, S. Nolin, A. Floyd, T. Mitchell ...

Source: NC State University Libraries
Added: August 6, 2018

2005 journal article

The genome sequence of the rice blast fungus Magnaporthe grisea

NATURE, 434(7036), 980–986.

By: R. Dean n , N. Talbot *, D. Ebbole *, M. Farman *, T. Mitchell n, M. Orbach *, M. Thon *, R. Kulkarni n ...

MeSH headings : Fungal Proteins / genetics; Fungal Proteins / metabolism; Genes, Fungal / genetics; Genome, Fungal; Guanosine Diphosphate / metabolism; Guanosine Triphosphate / metabolism; Magnaporthe / classification; Magnaporthe / genetics; Magnaporthe / metabolism; Magnaporthe / pathogenicity; Oryza / microbiology; Plant Diseases / microbiology; Point Mutation / genetics; Proteome / genetics; Proteome / metabolism; Receptors, G-Protein-Coupled / genetics; Repetitive Sequences, Nucleic Acid / genetics; Saccharomyces cerevisiae / metabolism; Signal Transduction; Virulence / genetics
TL;DR: The draft sequence of the M. grisea genome is reported, reflecting the clonal nature of this fungus imposed by widespread rice cultivation and analysis of the gene set provides an insight into the adaptations required by a fungus to cause disease. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

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