@article{ashwell_ceddia_house_cassady_eisen_eling_collins_grissom_odle_2010, title={Trans-10, cis-12-conjugated linoleic acid alters hepatic gene expression in a polygenic obese line of mice displaying hepatic lipidosis}, volume={21}, ISSN={["1873-4847"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-77955844665&partnerID=MN8TOARS}, DOI={10.1016/j.jnutbio.2009.06.013}, abstractNote={The trans-10, cis-12 isomer of conjugated linoleic acid (CLA) causes a rapid reduction of body and adipose mass in mice. In addition to changes in adipose tissue, numerous studies have reported alterations in hepatic lipid metabolism. Livers of CLA-fed mice gain mass, partly due to lipid accumulation; however, the precise molecular mechanisms are unknown. To elucidate these mechanisms, we examined fatty acid composition and gene expression profiles of livers from a polygenic obese line of mice fed 1% trans-10, cis-12-CLA for 14 days. Analysis of gene expression data led to the identification of 1393 genes differentially expressed in the liver of CLA-fed male mice at a nominal P value of .01, and 775 were considered significant using a false discovery rate (FDR) threshold of .05. While surprisingly few genes in lipid metabolism were impacted, pathway analysis found that protein kinase A (PKA) and cyclic adenosine monophosphate (cAMP) pathways signaling pathways were affected by CLA treatment and 98 of the 775 genes were found to be regulated by hepatocyte nuclear factor 4alpha, a transcription factor important in controlling liver metabolic status.}, number={9}, journal={JOURNAL OF NUTRITIONAL BIOCHEMISTRY}, author={Ashwell, Melissa S. and Ceddia, Ryan P. and House, Ralph L. and Cassady, Joseph P. and Eisen, Eugene J. and Eling, Thomas E. and Collins, Jennifer B. and Grissom, Sherry F. and Odle, Jack}, year={2010}, month={Sep}, pages={848–855} }
 @misc{house_cassady_eisen_mcintosh_odle_2005, title={Conjugated linoleic acid evokes de-lipidation through the regulation of genes controlling lipid metabolism in adipose and liver tissue}, volume={6}, ISSN={["1467-789X"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-22744451979&partnerID=MN8TOARS}, DOI={10.1111/j.1467-789X.2005.00198.x}, abstractNote={Summary}, number={3}, journal={OBESITY REVIEWS}, author={House, RL and Cassady, JP and Eisen, EJ and McIntosh, MK and Odle, J}, year={2005}, month={Aug}, pages={247–258} }
 @article{house_cassady_eisen_eling_collins_grissom_odle_2005, title={Functional genomic characterization of delipidation elicited by trans-10, cis-12-conjugated linoleic acid (t10c12-CLA) in a polygenic obese line of mice}, volume={21}, ISSN={["1531-2267"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-21244497638&partnerID=MN8TOARS}, DOI={10.1152/physiolgenomics.00244.2004}, abstractNote={Gene expression was measured during t10c12-CLA-induced body fat reduction in a polygenic obese line of mice. Adult mice ( n = 185) were allotted to a 2 × 2 factorial experiment consisting of either nonobese (ICR-control) or obese (M16-selected) mice fed a 7% fat, purified diet containing either 1% linoleic acid (LA) or 1% t10c12-CLA. Body weight (BW) by day 14 was 12% lower in CLA- compared with LA-fed mice ( P < 0.0001). By day 14, t10c12-CLA reduced weights of epididymal, mesenteric, and brown adipose tissues, as a percentage of BW, in both lines by 30, 27, and 58%, respectively, and increased liver weight/BW by 34% ( P < 0.0001). Total RNA was isolated and pooled (4 pools per tissue per day) from epididymal adipose ( days 5 and 14) of the obese mice to analyze gene expression profiles using Agilent mouse oligo microarray slides representing >20,000 genes. Numbers of genes differentially expressed by greater than or equal to twofold in epididymal adipose ( days 5 and 14) were 29 and 125, respectively. It was concluded that, in adipose tissue, CLA increased expression of uncoupling proteins (1 and 2), carnitine palmitoyltransferase system, tumor necrosis factor-α ( P < 0.05), and caspase-3 but decreased expression of peroxisome proliferator-activated receptor-γ, glucose transporter-4, perilipin, caveolin-1, adiponectin, resistin, and Bcl-2 ( P < 0.01). In conclusion, this experiment has revealed candidate genes that will be useful in elucidating mechanisms of adipose delipidation.}, number={3}, journal={PHYSIOLOGICAL GENOMICS}, author={House, RL and Cassady, JP and Eisen, EJ and Eling, TE and Collins, JB and Grissom, SF and Odle, J}, year={2005}, month={May}, pages={351–361} }
 @article{lin_house_odle_2005, title={Ontogeny and kinetics of carnitine palmitoyltransferase in liver and skeletal muscle of the domestic felid ()}, volume={16}, ISSN={0955-2863}, url={http://dx.doi.org/10.1016/j.jnutbio.2004.12.012}, DOI={10.1016/j.jnutbio.2004.12.012}, abstractNote={The ontogeny of carnitine palmitoyltransferase (CPT) was examined in liver and muscle throughout growth and development of the domestic felid. Homogenates from animals in six age categories (newborn, 24-h, 3-, 6- and 9-week-old, and adult) were examined. Hepatic CPT specific activity increased progressively from birth to 6 weeks and then declined slightly into adulthood, with maximal values for animals greater than 24 h of age [171 nmol/(min g wet tissue)] being 70% higher than for newborns [99 nmol/(min g wet tissue)] (P<.05). Specific activity in adults was similar to that in 6- and 9-week-old juveniles. Total hepatic CPT activity [nmol/(min liver)] increased linearly with age, but the activity expressed per kg body weight [nmol/(min kg BW)] declined after 3 weeks. In contrast, skeletal muscle CPT-specific activity remained unchanged from birth to 3 weeks and then increased significantly, with maximal values at 9 weeks being 90% greater than those for young animals (newborn to 3 weeks; P<.05), whereas specific activity in adults was 50% lower than that observed in 9-week-old animals (P<.05). Hepatic and muscle apparent Km's for carnitine averaged 440 μM and did not vary with age. Hepatic carnitine concentrations remained relatively constant during development, but were lower in adult lactating females, whereas skeletal muscle concentrations increased markedly with age. Hepatic concentrations were 20–50% higher than apparent Km's for carnitine in young and growing animals, but concentrations were similar to the apparent Km at 6 weeks and significantly lower than the apparent Km in adults. Carnitine concentrations in skeletal muscle were 37% lower than apparent Km during the neonatal period, but significantly higher in cats >3 weeks of age. We conclude that postnatal increases in CPT activity support increased capacity for fatty acid oxidation in the developing felid and that dietary carnitine may be required to maximize enzyme activity.}, number={6}, journal={The Journal of Nutritional Biochemistry}, publisher={Elsevier BV}, author={Lin, X and House, R and Odle, J}, year={2005}, month={Jun}, pages={331–338} }