@article{bringolf_heltsley_newton_eads_fraley_shea_cope_2010, title={ENVIRONMENTAL OCCURRENCE AND REPRODUCTIVE EFFECTS OF THE PHARMACEUTICAL FLUOXETINE IN NATIVE FRESHWATER MUSSELS}, volume={29}, ISSN={["1552-8618"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-77953583998&partnerID=MN8TOARS}, DOI={10.1002/etc.157}, abstractNote={The present study measured the occurrence, distribution, and bioaccumulation of fluoxetine in samples of water, polar organic chemical integrative sampler (POCIS), sediment, and caged freshwater mussels at stream sites near a municipal wastewater treatment facility effluent discharge. We assessed the relation of the environmental concentrations to reproductive endpoints in mussels in acute laboratory tests. Concentrations of fluoxetine in water and POCIS samples were similar (<20% difference) within each site and were greatest in the effluent channel (104-119 ng/L), and decreased at 50 m and 100 m downstream. Likewise, concentrations of fluoxetine in sediment and mussel (Elliptio complanata) tissue were greatest in the effluent channel (17.4 ng/g wet wt for sediment and 79.1 ng/g wet wt for mussels). In 96-h lab tests, fluoxetine significantly induced parturition of nonviable larvae from female E. complanata exposed to 300 microg/L (p = 0.0118) and 3,000 microg/L (p < 0.0001) compared to controls. Fluoxetine exposure at 300 microg/L (p = 0.0075) and 3,000 microg/L (p = 0.0001) also resulted in stimulation of lure display behavior in female Lampsilis fasciola and Lampsilis cardium, respectively. In male E. complanata, 3,000 microg fluoxetine/L significantly induced release of spermatozeugmata during a 48-h exposure. These results suggest that fluoxetine accumulates in mussel tissue and has the potential to disrupt several aspects of reproduction in freshwater mussels, a faunal group recognized as one of the most imperiled in the world. Despite the disparity between measured environmental concentrations of fluoxetine and effects concentrations in our short-term tests with these long-lived animals, additional tests are warranted to evaluate the effects of long-term exposure to environmentally relevant concentrations and critical lifestages (e.g., juveniles).}, number={6}, journal={ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY}, author={Bringolf, Robert B. and Heltsley, Rebecca M. and Newton, Teresa J. and Eads, Chris B. and Fraley, Stephen J. and Shea, Damian and Cope, W. Gregory}, year={2010}, month={Jun}, pages={1311–1318} }
 @article{heltsley_cope_shea_bringolf_kwak_malindzak_2005, title={Assessing organic contaminants in fish: Comparison of a nonlethal tissue sampling technique to mobile and stationary passive sampling devices}, volume={39}, ISSN={["1520-5851"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-26044466979&partnerID=MN8TOARS}, DOI={10.1021/es051037s}, abstractNote={As concerns mount over the human health risks associated with consumption of fish contaminated with persistent organic pollutants, there exists a need to better evaluate fish body burdens without lethally sampling many of the important commercial and sport species of interest. The aim of this study was to investigate two novel methods for estimating organic contaminants in fish that are a concern for both fish and human health. The removal of fish adipose fins, commonly done in mark-recapture studies with salmonid species, was evaluated as a nonlethal sampling technique to estimate concentrations of polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCPs) in flathead catfish (Pylodictis olivaris), relative to those found in muscle fillets of the same fish. We also assessed the efficacy of using poly(dimethylsiloxane) (PDMS) as a mobile passive sampling device (PSD) attached directly to wild flathead catfish for assessing location-specific exposure of the fish to waterborne contaminants. The results of this study have demonstrated for the first time that organic contaminant concentrations in adipose fin were highly correlated (R2 = 0.87) with muscle fillet concentrations, indicating that the adipose fin of certain fishes may be used to accurately estimate tissue concentrations without the need for lethal sampling. Moreover, mobile PSDs attached directly to fish and used here for the first time accurately estimated ultratrace concentrations of waterborne PCBs and OCPs without any apparent harm to the fish, indicating that there are no practical or physical barriers to the use of mobile passive samplers attached to aquatic organisms. Among the many practical implications of this research, two potential priority items include the analysis of organic contaminants in farm-raised and sport fish intended for human consumption, without the economic and population losses associated with lethally sampling fish to obtain tissues, and identifying specific areas where fish may be accumulating large portions of their contaminant burden.}, number={19}, journal={ENVIRONMENTAL SCIENCE & TECHNOLOGY}, author={Heltsley, RM and Cope, WG and Shea, D and Bringolf, RB and Kwak, TJ and Malindzak, EG}, year={2005}, month={Oct}, pages={7601–7608} }