Syed Salman Ashraf

Works (9)

Updated: July 5th, 2023 16:02

2005 patent

Antibacterial and antiviral agents and methods of screening for the same

Washington, DC: U.S. Patent and Trademark Office.

By: P. Agris & S. Ashraf

Source: NC State University Libraries
Added: August 6, 2018

2000 journal article

Role of modified nucleosides of yeast tRNA(Phe) in ribosomal binding

CELL BIOCHEMISTRY AND BIOPHYSICS, 33(3), 241–252.

By: S. Ashraf*, R. Guenther n, G. Ansari n, A. Malkiewicz*, E. Sochacka * & P. Agris n

author keywords: tRNA(Phe); anticodon; ASL; nucleoside modification; ribosome
MeSH headings : Nucleosides / genetics; Nucleosides / metabolism; RNA, Fungal / genetics; RNA, Fungal / metabolism; RNA, Transfer, Phe / genetics; RNA, Transfer, Phe / metabolism; Ribosomes / metabolism; Saccharomyces cerevisiae / genetics; Saccharomyces cerevisiae / metabolism
TL;DR: Effective ribosome binding of tRNAPhe is a combination of anticodon stem stability and the correct architecture and dynamics of the anticodon loop, and 2′O-methylation of guanosine at wobble position 34 (Gm34) is identified as being responsible for the characteristically enhanced chemical reactivity of C1400 in Escherichia coli 16S rRNA upon ribosomal footprinting of yeast t RNAPhe. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

1999 patent

Antibacterial agents and methods of screening for the same

Washington, DC: U.S. Patent and Trademark Office.

By: P. Agris & S. Ashraf

Source: NC State University Libraries
Added: August 6, 2018

1999 journal article

Orientation of the tRNA anticodon in the ribosomal P-site: Quantitative footprinting with U-33-modified, anticodon stem and loop domains

RNA, 5(9), 1191–1199.

By: S. Ashraf n, R. Guenther n & P. Agris n

author keywords: anticodon stem/loops; modified nucleosides; quantitative footprinting; ribosome
MeSH headings : Aldehydes / pharmacology; Anticodon / genetics; Antiviral Agents / pharmacology; Base Sequence; Butanones; Dose-Response Relationship, Drug; Genetic Techniques; Kinetics; Molecular Sequence Data; Mutagens / pharmacology; RNA, Fungal / genetics; RNA, Fungal / metabolism; RNA, Ribosomal, 16S / metabolism; RNA, Transfer / genetics; RNA, Transfer / metabolism; RNA, Transfer, Phe / metabolism; Ribosomes / genetics; Ribosomes / metabolism; Sulfuric Acid Esters / pharmacology; Temperature
TL;DR: Two residues, G926 and G1338, were afforded significantly greater protection by both yeast tRNA(Phe) and the ASL-U33 than other residues, such as A532, A794, C795, and A1339, which are important to efficient P-site binding of tRNA. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

1999 journal article

Single atom modification (O -> S) of tRNA confers ribosome binding

RNA, 5(2), 188–194.

By: S. Ashraf n, E. Sochacka *, R. Cain n, R. Guenther n, A. Malkiewicz* & P. Agris n

author keywords: 2-thiouridine; anticodon conformation; anticodon stem loop analogs; nucleoside modifications; ribosome; translation; tRNA(LyS)
MeSH headings : Aldehydes / metabolism; Anticodon / genetics; Butanones; Escherichia coli / metabolism; Humans; Magnetic Resonance Spectroscopy; Nucleic Acid Conformation; Nucleic Acid Denaturation / genetics; Nucleic Acid Hybridization / genetics; RNA, Fungal / genetics; RNA, Transfer, Lys / chemistry; RNA, Transfer, Lys / genetics; Ribosomes / metabolism; Spectrum Analysis; Temperature; Thiouridine / analogs & derivatives; Thiouridine / metabolism
TL;DR: It is concluded that s2U34 in tRNALysSUU and in other s2 U34-containing tRNAs is critical for generating an anticodon conformation that leads to effective codon interaction in all organisms. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

1999 journal article

The uridine in "U-turn": Contributions to tRNA-ribosomal binding

RNA, 5(4), 503–511.

By: S. Ashraf n, G. Ansari n, R. Guenther n, E. Sochacka *, A. Malkiewicz* & P. Agris n

author keywords: anticodon conformation; anticodon stem loop analogs; ASL; nucleoside modifications; ribosome; translation; tRNA(Phe) U-turn
MeSH headings : Anticodon / chemistry; Anticodon / genetics; Humans; Models, Molecular; Molecular Structure; Nucleic Acid Conformation; Nucleic Acid Denaturation; RNA, Fungal / chemistry; RNA, Fungal / genetics; RNA, Transfer, Phe / chemistry; RNA, Transfer, Phe / metabolism; Ribosomes / genetics; Ribosomes / metabolism; Temperature; Uridine / chemistry; Uridine / genetics
TL;DR: The results demonstrate that proton donations by the N3-H and 2'OH groups of U33 are not absolutely required for ribosomal binding, and suggest that the overall uridine conformation, including a dynamic (C3'-endo > C2'-endo) sugar pucker, anti-conformation, and ability of uracil to stack between C32 and A35-phosphate, are the contributing factors to a functional U-turn. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

1997 conference paper

Ribosomal binding of modified tRNA anticodons related to thermal stability

Symposium on RNA Biology II. RNA: Tool and Target (1997: North Carolina Biotechnology Center) Research Triangle Park, North Carolina, USA, October 17-19, 1997 (Nucleic acids symposium series; no. 36), 36, 58–60. Oxford: Oxford University Press.

By: S. Ashraf, R. Guenther, W. Ye, Y. Lee, A. Malkiewicz & P. Agris

Source: NC State University Libraries
Added: August 6, 2018

1996 patent

Purification of hemoglobin by displacement chromatography

Washington, DC: U.S. Patent and Trademark Office.

By: D. Pliura, D. Wiffen, S. Ashraf & A. Magnin

Source: NC State University Libraries
Added: August 6, 2018

1995 patent

Displacement chromatography process

Washington, DC: U.S. Patent and Trademark Office.

By: D. Pliura, D. Wiffen, S. Ashraf & A. Magnin

Source: NC State University Libraries
Added: August 6, 2018

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