@article{teller_boyd_lecompte_kennett_west_telka_diaz_adedeji_batchelor_mooney_et al._2020, title={A multi-proxy study of changing environmental conditions in a Younger Dryas sequence in southwestern Manitoba, Canada, and evidence for an extraterrestrial event}, volume={93}, ISSN={["1096-0287"]}, DOI={10.1017/qua.2019.46}, abstractNote={AbstractMulti-proxy analyses of a sequence spanning the Younger Dryas (YD) in the Glacial Lake Hind basin of Manitoba provides insight into regional paleohydrology and paleovegetation of meltwater rivers and lakes spanning >4000 yr; the sequence is controlled by 25 new accelerator mass spectrometry ages. This lake, dammed by the Laurentide Ice Sheet, overflowed into Lake Agassiz. The pre-YD interval records rapid sedimentation from meltwaters that headed in proglacial lakes in the Canadian Prairies that are known to have been catastrophically released when ice or sediment barriers were breached. Pollen in this phase is dominated by pre-Quaternary forms eroded from Paleocene bedrock. At the onset of the YD at ~12.8 cal ka, the sudden appearance of concentrations of nanodiamonds, high-temperature magnetic spherules, platinum, and iridium provide evidence of an extraterrestrial (ET) event that others have identified at more than 40 sites in North America. Major changes in oceans and climate, and the catastrophic outflow of nearby Lake Agassiz at the onset of the YD, may be related. Lower water levels and a reduction of Souris River inflow to Lake Hind followed, which are reflected by more clayey and organic-rich sediments and a decrease in pre-Quaternary palynomorphs. This may have resulted from the deepening of river valleys caused by the release of meltwater triggered by the ET event. Wetlands then began to develop, leading to peat deposition from 12.3 to 11 cal ka. This was followed by a fluvial episode depositing sand and then by increased Holocene aridity that resulted in accumulation of a thick sequence of dune sands. A dry woodland environment with a mix of conifers (especially Picea and Larix) and deciduous trees (especially Populus and Quercus) covered the uplands from ~13 to 10 cal ka.}, number={1}, journal={QUATERNARY RESEARCH}, author={Teller, James and Boyd, Matthew and LeCompte, Malcolm and Kennett, James and West, Allen and Telka, Alice and Diaz, Aura and Adedeji, Victor and Batchelor, Dale and Mooney, Charles and et al.}, year={2020}, month={Jan}, pages={60–87} } @article{pino_abarzua_astorga_martel-cea_cossio-montecinos_navarro_lira_labarca_lecompte_adedeji_et al._2019, title={Sedimentary record from Patagonia, southern Chile supports cosmic-impact triggering of biomass burning, climate change, and megafaunal extinctions at 12.8 ka}, volume={9}, ISSN={["2045-2322"]}, DOI={10.1038/s41598-018-38089-y}, abstractNote={AbstractThe Younger Dryas (YD) impact hypothesis posits that fragments of a large, disintegrating asteroid/comet struck North America, South America, Europe, and western Asia ~12,800 years ago. Multiple airbursts/impacts produced the YD boundary layer (YDB), depositing peak concentrations of platinum, high-temperature spherules, meltglass, and nanodiamonds, forming an isochronous datum at >50 sites across ~50 million km² of Earth’s surface. This proposed event triggered extensive biomass burning, brief impact winter, YD climate change, and contributed to extinctions of late Pleistocene megafauna. In the most extensive investigation south of the equator, we report on a ~12,800-year-old sequence at Pilauco, Chile (~40°S), that exhibits peak YD boundary concentrations of platinum, gold, high-temperature iron- and chromium-rich spherules, and native iron particles rarely found in nature. A major peak in charcoal abundance marks an intense biomass-burning episode, synchronous with dramatic changes in vegetation, including a high-disturbance regime, seasonality in precipitation, and warmer conditions. This is anti-phased with northern-hemispheric cooling at the YD onset, whose rapidity suggests atmospheric linkage. The sudden disappearance of megafaunal remains and dung fungi in the YDB layer at Pilauco correlates with megafaunal extinctions across the Americas. The Pilauco record appears consistent with YDB impact evidence found at sites on four continents.}, journal={SCIENTIFIC REPORTS}, author={Pino, Mario and Abarzua, Ana M. and Astorga, Giselle and Martel-Cea, Alejandra and Cossio-Montecinos, Nathalie and Navarro, R. Ximena and Lira, Maria Paz and Labarca, Rafael and LeCompte, Malcolm A. and Adedeji, Victor and et al.}, year={2019}, month={Mar} } @article{flickinger_bernal_schulte_broglie_duran_wallace_mooney_velev_2017, title={Biocoatings: challenges to expanding the functionality of waterborne latex coatings by incorporating concentrated living microorganisms}, volume={14}, ISSN={["1935-3804"]}, DOI={10.1007/s11998-017-9933-6}, number={4}, journal={JOURNAL OF COATINGS TECHNOLOGY AND RESEARCH}, author={Flickinger, Michael C. and Bernal, Oscar I. and Schulte, Mark J. and Broglie, Jessica Jenkins and Duran, Christopher J. and Wallace, Adam and Mooney, Charles B. and Velev, Orlin D.}, year={2017}, month={Jul}, pages={791–808} } @article{schulte_wiltgen_ritter_mooney_flickinger_2016, title={A High Gas Fraction, Reduced Power, Syngas Bioprocessing Method Demonstrated With a Clostridium ljungdahlii OTA1 Paper Biocomposite}, volume={113}, ISSN={["1097-0290"]}, DOI={10.1002/bit.25966}, abstractNote={ABSTRACTWe propose a novel approach to continuous bioprocessing of gases. A miniaturized, coated‐paper strip, high gas fraction, biocomposite absorber has been developed using slowly shaken horizontal anaerobic tubes. Concentrated Clostridium ljungdahlii OTA1 was used as a model system. These gas absorbers demonstrate elevated CO mass transfer with low power input, reduced liquid requirements, elevated substrate consumption, and increased product secretion compared to shaken suspended cells. Concentrated OTA1 cell paste was coated by extrusion onto chromatography paper. The immobilized system shows high, constant reactivity immediately upon rehydration. Cell adhesion was by adsorption to the cellulose fibers; visualized by SEM. The C. ljungdahlii OTA1 coated paper mounted above the liquid level absorbs CO and H2 from a model syngas secreting acetate with minimal ethanol. At 100 rpm shaking speed (7.7 Wm−3) the optimal cell loading is 6.5 gDCW m−2 to maintain high CO absorbing reactivity without the cells coming off of the paper into the liquid phase. Reducing the medium volume from 10 mL to 4 mL (15% of tube volume) did not decrease CO reactivity. The reduced liquid volume increased secreted product concentration by 80%. The specific CO consumption by paper biocomposites was higher at all shaking frequencies <100 rpm than suspended cells under identical incubation conditions. At 25 rpm the biocomposite outperforms suspended cells for CO absorption by 2.5‐fold, with an estimated power reduction of 97% over the power input at 100 rpm. The estimated minimum kLa for miniaturized biocomposite gas‐absorbers is ∼100 h−1, 10 to 104 less power input than other syngas fermentation systems reported in the literature at similar kLa. Specific consumption rates in a biocomposite were ∼14 mmol h−1. This work intensified CO absorption and reactivity by 14‐fold to 94 mmol CO m−2 h−1 over previous C. ljungdahlii OTA1 work by our group. Specific acetate production rates were 23 mM h−1 or 46 mmol m−2 h−1. The specific rates and apparent kLa scaled linearly with biocomposite coating area. Biotechnol. Bioeng. 2016;113: 1913–1923. © 2016 Wiley Periodicals, Inc.}, number={9}, journal={BIOTECHNOLOGY AND BIOENGINEERING}, author={Schulte, Mark J. and Wiltgen, Jeff and Ritter, John and Mooney, Charles B. and Flickinger, Michael C.}, year={2016}, month={Sep}, pages={1913–1923} } @article{niemuth_sanders_mooney_olfenbuttel_deperno_stoskopf_2014, title={Nephrolithiasis in free-ranging North American river otter (lontra canadensis) in North Carolina, USA}, volume={45}, ISSN={1042-7260 1937-2825}, url={http://dx.doi.org/10.1638/2013-0135r2.1}, DOI={10.1638/2013-0135r2.1}, abstractNote={Abstract: The North American river otter (Lontra canadensis) serves as an indicator species for environmental monitoring, is prized as a valuable furbearer, and is a popular display animal in zoologic collections. Nephrolithiasis has been reported as a frequent problem in other free-ranging and captive otter species but is rarely reported in North American river otters. In this study, we compared the prevalence of nephrolithiasis diagnosed using routine gross pathologic examination techniques with the use of computed tomography (CT) of excised kidneys. We also evaluated whether otter nephroliths could be accurately classified by their CT densities, and we examined the renal tissue uric acid concentrations in free-ranging otters in North Carolina, USA. Kidneys were collected from carcasses of legally trapped, free-ranging animals. Nephroliths were observed in 16.2% of the individuals (n = 229). Associations were found between age and nephrolith status and between capture location and nephrolith status (P = 0.026 and < 0.001, respectively). Computed tomography Hounsfield unit density measurements were not useful in determining nephrolith chemical composition in this study. Renal tissue uric acid concentrations were similar across genders, age groups, and stone status. The chemical composition of the nephroliths was determined by scanning electron microscopy–energy dispersive X-ray spectroscopy to be calcium phosphate in the carbonate form.}, number={1}, journal={Journal of Zoo and Wildlife Medicine}, publisher={American Association of Zoo Veterinarians}, author={Niemuth, Jennifer N. and Sanders, Charles W. and Mooney, Charles B. and Olfenbuttel, Colleen and DePerno, Christopher S. and Stoskopf, Michael K.}, year={2014}, month={Mar}, pages={110–117} } @article{bernal_mooney_flickinger_2014, title={Specific Photosynthetic Rate Enhancement by Cyanobacteria Coated Onto Paper Enables Engineering of Highly Reactive Cellular Biocomposite "Leaves"}, volume={111}, ISSN={["1097-0290"]}, DOI={10.1002/bit.25280}, abstractNote={ABSTRACTWe describe a latex wet coalescence extrusive coating method that produces up to 10‐fold specific photosynthetic rate enhancements by nitrate‐limited non‐growing cyanobacteria deposited onto paper, hydrated and placed in the gas‐phase of small tube photobioreactors. These plant leaf‐like biocomposites were used to study the tolerance of cyanobacteria strains to illumination and temperature using a solar simulator. We report sustained CO2 absorption and O2 production for 500 h by hydrated gas‐phase paper coatings of non‐growing Synechococcus PCC7002, Synechocystis PCC6803, Synechocystis PCC6308, and Anabaena PCC7120. Nitrate‐starved cyanobacteria immobilized on the paper surface by the latex binder did not grow out of the coatings into the bulk liquid. The average CO2 consumption rate in Synechococcus coatings is 5.67 mmol m−2 h−1 which is remarkably close to the rate reported in the literature for Arabidopsis thaliana leaves under similar experimental conditions (18 mmol m−2 h−1). We observed average ratios of oxygen production to carbon dioxide consumption (photosynthetic quotient, PQ) between 1.3 and 1.4, which may indicate a strong dependence on nitrate assimilation during growth and was used to develop a non‐growth media formulation for intrinsic kinetics studies. Photosynthetic intensification factors (PIF) (O2 production by nitrate‐limited cyanobacteria in latex coatings/O2 produced by nitrate‐limited cell suspensions) in cyanobacteria biocomposites prepared from wet cell pellets concentrated 100‐ to 300‐fold show 7–10 times higher specific reactivity compared to cells in suspension under identical nitrate‐limited non‐growth conditions. This is the first report of changes of cyanobacteria tolerance to temperature and light intensities after deposition as a thin coating on a porous matrix, which has important implications for gas‐phase photobioreactor design using porous composite materials. Cryo‐fracture SEM and confocal microscopy images of cell coating distribution on the paper biocomposite suggest that the spatial arrangement of the cells in the coating can affect photoreactivity. This technique could be used to fabricate very stable, multi‐organism composite coatings on flexible microfluidic devices in the gas‐phase capable of harvesting light in a broader range of wavelengths, to optimize thermotolerant, desiccation tolerant, or halotolerant cyanobacteria that produce O2 with secretion of liquid‐fuel precursors synthesized from CO2. Biotechnol. Bioeng. 2014;111: 1993–2008. © 2014 Wiley Periodicals, Inc.}, number={10}, journal={BIOTECHNOLOGY AND BIOENGINEERING}, author={Bernal, Oscar I. and Mooney, Charles B. and Flickinger, Michael C.}, year={2014}, month={Oct}, pages={1993–2008} } @misc{lecompte_batchelor_demitroff_vogel_mooney_rock_seidel_2013, title={Reply to Boslough: Prior studies validating research are ignored}, volume={110}, number={18}, journal={Proceedings of the National Academy of Sciences of the United States of America}, author={LeCompte, M. A. and Batchelor, D. and Demitroff, M. N. and Vogel, E. K. and Mooney, C. and Rock, B. N. and Seidel, A. W.}, year={2013}, pages={E1652–1652} } @article{lecompte_goodyear_demitroff_batchelor_vogel_mooney_rock_seidel_2012, title={Independent evaluation of conflicting microspherule results from different investigations of the Younger Dryas impact hypothesis}, volume={109}, number={44}, journal={Proceedings of the National Academy of Sciences of the United States of America}, author={LeCompte, M. A. and Goodyear, A. C. and Demitroff, M. N. and Batchelor, D. and Vogel, E. K. and Mooney, C. and Rock, B. N. and Seidel, A. W.}, year={2012}, pages={E2960–2969} } @article{shi_lane_mooney_dow_scattergood_2010, title={Diamond tool wear measurement by electron-beam-induced deposition}, volume={34}, ISSN={["1873-2372"]}, DOI={10.1016/j.precisioneng.2010.03.009}, abstractNote={Quantitative characterization of a diamond tool profile is critical to reveal tool wear mechanisms. An electron-beam-induced deposition (EBID) method reported previously is further developed and improved to measure diamond tool profiles using a field emission scanning electron microscope (SEM). The edge radius and wear land length for new and worn diamond tools were derived from analysis of the EBID-SEM images. Experimental results are presented to show that the methodology is an effective means to characterize diamond tool wear.}, number={4}, journal={PRECISION ENGINEERING-JOURNAL OF THE INTERNATIONAL SOCIETIES FOR PRECISION ENGINEERING AND NANOTECHNOLOGY}, author={Shi, M. and Lane, B. and Mooney, C. B. and Dow, T. A. and Scattergood, R. O.}, year={2010}, month={Oct}, pages={718–721} } @article{kitamura_yonei_iwatsuki_mooney_fukuda_2005, title={Mapping contact potential differences with noncontact atomic force microscope using resonance frequency shift versus sample bias voltage curves}, volume={44}, number={11}, journal={Japanese Journal of Applied Physics. Part 1, Regular Papers, Short Notes & Review Papers}, author={Kitamura, S. and Yonei, K. and Iwatsuki, M. and Mooney, C. B. and Fukuda, Y.}, year={2005}, pages={8113–8115} }