@article{olivry_bizikova_dunston_bond_halliwell_loeffler_pucheu-haston_chen_marinkovich_2010, title={Clinical and immunological heterogeneity of canine subepidermal blistering dermatoses with anti-laminin-332 (laminin-5) auto-antibodies}, volume={21}, number={4}, journal={Veterinary Dermatology}, author={Olivry, T. and Bizikova, P. and Dunston, S. M. and Bond, R. and Halliwell, R. and Loeffler, A. and Pucheu-Haston, C. M. and Chen, M. and Marinkovich, M. P.}, year={2010}, pages={345–357} }
 @article{pucheu-haston_jackson_olivry_dunston_hammerberg_2008, title={Epicutaneous sensitization with Dermatophagoides farinae induces generalized allergic dermatitis and elevated mite-specific immunoglobulin E levels in a canine model of atopic dermatitis}, volume={38}, ISSN={["0954-7894"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-40949126521&partnerID=MN8TOARS}, DOI={10.1111/j.1365-2222.2008.02949.x}, abstractNote={SummaryBackground
					 Atopic dermatitis (AD) is a cutaneous hypersensitivity associated with elevated levels of antigen‐specific IgE, commonly to house dust mites (HDMs). It remains controversial as to whether sensitization and clinical disease are induced by cutaneous exposure to HDM.Objectives
					 The objectives of this study were to determine whether repeated applications of Dermatophagoides farinae slurry to intact skin of Maltese–Beagle atopic (MAB) dogs would result in the development of clinical signs or lesions resembling spontaneous canine AD, to determine whether repeated slurry applications would induce elevations in mite‐specific IgE and/or IgG, and to determine whether mite antigens could be demonstrated within the dermis of application sites.Methods
					 Dogs received weekly slurry applications to the axilla and groin, and were patch tested at 120 days, or were patch tested at days 1, 60 and 120, but did not receive further slurry applications. Skin biopsies and serum samples were obtained on days 1, 60 and 120.Results
					 Pruritic dermatitis was seen in all dogs by day 60. D. farinae‐specific IgE was elevated by day 60. Histologic examination of early application sites revealed mild, mononuclear perivascular dermatitis. Later application sites were characterized by a dense inflammatory infiltrate and oedema in both the dermis and the epidermis. Immunofluorescent staining confirmed the presence of D. farinae antigens in the dermis.Conclusions
					 This study demonstrated that epicutaneous application of HDM slurry to MAB dogs results in elevations of HDM‐specific IgE, localized and generalized pruritic dermatitis resembling spontaneous canine AD, and histologic changes typical of IgE‐driven inflammation. We feel that these results suggest that epicutaneous exposure to allergen may play an important role during both the sensitization and the perpetuation of AD, and provide support for the use of a canine model in the investigation of the pathogenesis of AD.}, number={4}, journal={CLINICAL AND EXPERIMENTAL ALLERGY}, author={Pucheu-Haston, C. M. and Jackson, H. A. and Olivry, T. and Dunston, S. M. and Hammerberg, B.}, year={2008}, month={Apr}, pages={667–679} }
 @article{pucheu-haston_shuster_olivry_brianceau_lockwood_mcclanahan_malefyt_mattson_hammerberg_2006, title={A canine model of cutaneous late-phase reactions: prednisolone inhibition of cellular and cytokine responses}, volume={117}, ISSN={["1365-2567"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-33645064542&partnerID=MN8TOARS}, DOI={10.1111/j.1365-2567.2005.02276.x}, abstractNote={SummaryImmunoglobulin E (IgE)‐mediated late‐phase reactions can be induced in atopic humans by intradermal injection of relevant allergens or anti‐IgE antibodies. The histology of these reactions resembles that of naturally occurring atopic dermatitis. Strikingly similar responses can be induced in dogs, suggesting that a canine model could prove valuable for preclinical investigation of drugs targeting late‐phase reactions. This study was designed to characterize the cellular, cytokine and chemokine responses after intradermal anti‐IgE injection in untreated and prednisolone‐treated dogs. Normal beagles were untreated or treated with prednisolone before intradermal injection of polyclonal rabbit anti‐canine IgE or normal rabbit IgG. Biopsies were taken before injection and 6, 24 and 48 hr after injection. Samples were evaluated by histological and immunohistochemical staining, as well as by real‐time quantitative polymerase chain reaction analysis. Dermal eosinophil and neutrophil numbers increased dramatically within 6 hr after injection of rabbit anti‐canine IgE, and remained moderately elevated at 48 hr. The numbers of CD1c+ and CD3+ mononuclear cells were also increased at 6 hr. The real‐time quantitative polymerase chain reaction demonstrated marked increases in mRNA expression for interleukin‐13 (IL‐13), CCL2, CCL5 and CCL17. Levels of mRNA for IL‐2, IL‐4, IL‐6 and IFN‐γ did not change within the limits of detection. Prednisolone administration suppressed the influx of neutrophils, eosinophils, CD1c+ and CD3+ cells, as well as expression of IL‐13, CCL2, CCL5 and CCL17. These data document the cytokine and chemokine responses to anti‐IgE injection in canine skin, and they demonstrate the ability of the model to characterize the anti‐inflammatory effects of a known therapeutic agent.}, number={2}, journal={IMMUNOLOGY}, author={Pucheu-Haston, CM and Shuster, D and Olivry, T and Brianceau, P and Lockwood, P and McClanahan, T and Malefyt, RD and Mattson, JD and Hammerberg, B}, year={2006}, month={Feb}, pages={177–187} }
 @article{pucheu-haston_grier_esch_bevier_1996, title={Allergenic cross-reactivities in flea-reactive canine serum samples}, volume={57}, number={7}, journal={American Journal of Veterinary Research}, author={Pucheu-Haston, C. M. and Grier, T. J. and Esch, R. E. and Bevier, D. E.}, year={1996}, pages={1000} }