@article{hu_wu_dalal_vasani_lopez_sederoff_qu_2017, title={Accumulation of medium-chain, saturated fatty acyl moieties in seed oils of transgenic Camelina sativa}, volume={12}, ISSN={["1932-6203"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-85013067776&partnerID=MN8TOARS}, DOI={10.1371/journal.pone.0172296}, abstractNote={With its high seed oil content, the mustard family plant Camelina sativa has gained attention as a potential biofuel source. As a bioenergy crop, camelina has many advantages. It grows on marginal land with low demand for water and fertilizer, has a relatively short life cycle, and is stress tolerant. As most other crop seed oils, camelina seed triacylglycerols (TAGs) consist of mostly long, unsaturated fatty acyl moieties, which is not desirable for biofuel processing. In our efforts to produce shorter, saturated chain fatty acyl moieties in camelina seed oil for conversion to jet fuel, a 12:0-acyl-carrier thioesterase gene, UcFATB1, from California bay (Umbellularia californica Nutt.) was expressed in camelina seeds. Up to 40% of short chain laurate (C12:0) and myristate (C14:0) were present in TAGs of the seed oil of the transgenics. The total oil content and germination rate of the transgenic seeds were not affected. Analysis of positions of these two fatty acyl moieties in TAGs indicated that they were present at the sn-1 and sn-3 positions, but not sn-2, on the TAGs. Suppression of the camelina KASII genes by RNAi constructs led to higher accumulation of palmitate (C16:0), from 7.5% up to 28.5%, and further reduction of longer, unsaturated fatty acids in seed TAGs. Co-transformation of camelina with both constructs resulted in enhanced accumulation of all three medium-chain, saturated fatty acids in camelina seed oils. Our results show that a California bay gene can be successfully used to modify the oil composition in camelina seed and present a new biological alternative for jet fuel production.}, number={2}, journal={PLOS ONE}, author={Hu, Zhaohui and Wu, Qian and Dalal, Jyoti and Vasani, Naresh and Lopez, Harry O. and Sederoff, Heike W. and Qu, Rongda}, year={2017}, month={Feb} }
 @article{wu_shanthraj_zikry_2013, title={Modeling the heterogeneous effects of retained austenite on the behavior of martensitic high strength steels}, volume={184}, DOI={10.1007/978-3-319-04397-5_16}, number={1-2}, journal={International Journal of Fracture}, author={Wu, Q. and Shanthraj, P. and Zikry, Mohammed}, year={2013}, pages={241–252} }
 @article{khodakovskaya_sword_wu_perera_boss_brown_sederoff_2010, title={Increasing inositol (1,4,5)-trisphosphate metabolism affects drought tolerance, carbohydrate metabolism and phosphate-sensitive biomass increases in tomato}, volume={8}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-73949085190&partnerID=MN8TOARS}, DOI={10.1111/j.1467-7652.2009.00472.x}, abstractNote={Summary}, number={2}, journal={Plant Biotechnology Journal}, author={Khodakovskaya, M. and Sword, C. and Wu, Q. and Perera, I. Y. and Boss, W. F. and Brown, C. S. and Sederoff, Heike}, year={2010}, pages={170–183} }
 @article{filichkin_wu_busov_meilan_lanz-garcia_groover_goldfarb_ma_dharmawardhana_brunner_et al._2006, title={Enhancer trapping in woody plants: Isolation of the ET304 gene encoding a putative AT-hook motif transcription factor and characterization of the expression patterns conferred by its promoter in transgenic Populus and Arabidopsis}, volume={171}, ISSN={["0168-9452"]}, DOI={10.1016/j.plantsci.2006.03.011}, abstractNote={Enhancer trapping is a useful tool in isolation of novel genes and functional characterization of promoters directing tissue-specific expression in trees. Using an enhancer trap approach we isolated a novel gene ET304 from Populus (Populus: aspens and cottonwoods). Both ET304 enhancer trap line and putative ET304 promoter fused to a GUS reporter gene conferred strongly root-predominant expression patterns and directed expression to specific root tissue and cell types. GUS activity was detected in lateral root primordia, root apical meristem, elongation zone and cortex. ET304 promoter sequence contained a canonic auxin response element (AuxRE) located upstream of the enhancer trap insertion site. In a synchronized lateral root induction system ET304 promoter conferred an auxin-responsive expression in newly emerging lateral roots of both poplar and Arabidopsis. A detailed histochemical examination of poplar transgenics showed that ET304 promoter was highly active in actively growing lateral roots, their primordia and to a lesser extent—in secondary meristems of aerial organs rich in free endogenous auxin. These results were consistent with the expression profiling of ET304 mRNA in various tissues of mature poplar trees. The analysis of Populus genomic sequence suggested that ET304 represents a large family of putative transcription factors containing a conserved AT-hook motif and DNA binding domain.}, number={2}, journal={PLANT SCIENCE}, author={Filichkin, Sergei A. and Wu, Qian and Busov, Victor and Meilan, Richard and Lanz-Garcia, Carmen and Groover, Andrew and Goldfarb, Barry and Ma, Caiping and Dharmawardhana, Palitha and Brunner, Amy and et al.}, year={2006}, month={Aug}, pages={206–216} }
 @article{mozdziak_wu_bradford_pardue_borwornpinyo_giamario_petitte_2006, title={Identification of the lacZ insertion site and beta-galactosidase expression in transgenic chickens}, volume={324}, ISSN={["1432-0878"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-33644623527&partnerID=MN8TOARS}, DOI={10.1007/s00441-005-0060-9}, abstractNote={The quail:chick chimera system is a classical research model in developmental biology. An improvement over the quail:chick chimera system would be a line of transgenic chickens expressing a reporter gene. Transgenic chickens carrying lacZ and expressing bacterial beta-galactosidase have been generated, but complete characterization of the insertion event and characterization of beta-galactosidase expression have not previously been available. The genomic sequences flanking the retroviral insertion site have now been identified by using inverse polymerase chain reaction (PCR), homozygous individuals have been identified by using PCR-based genotyping, and beta-galactosidase expression has been evaluated by using Western analysis and histochemistry. Based upon the current draft of the chicken genome, the viral insertion carrying the lacZ gene has been located on chromosome 11 within the predicted gene for neurotactin/fractalkine (CX3CL1); neurotactin mRNA expression appears to be missing from the brain of homozygous individuals. When Generation 2 (G2) lacZ-positive individuals were inter-mated, they generated 361 G3 progeny; 82 were homozyous for lacZ (22.7%), 97 were wild-type non-transgenic (26.9%), and 182 (50.4%) were hemizygous for lacZ. Western analysis revealed the highest expression in the muscle and liver. With the identification of homozygous birds, the line of chickens is now designated NCSU-Blue1.}, number={1}, journal={CELL AND TISSUE RESEARCH}, author={Mozdziak, PE and Wu, Q and Bradford, JM and Pardue, SL and Borwornpinyo, S and Giamario, C and Petitte, JN}, year={2006}, month={Apr}, pages={41–53} }