Prashant K. Nighot

Works (15)

Updated: August 20th, 2023 21:19

2016 conference paper

Parametric study of a fluidic artificial muscle actuated electrohydraulic system

Proceedings of the asme conference on smart materials adaptive.

By: E. Chapman n, T. Jenkins n & M. Bryant n

Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

2015 journal article

Genetic Ablation of the ClC-2 Cl- Channel Disrupts Mouse Gastric Parietal Cell Acid Secretion

PLOS ONE, 10(9), e0138174.

By: M. Nighot n, P. Nighot n, T. Ma *, D. Malinowska*, G. Shull*, J. Cuppoletti*, A. Blikslager n 

Contributors: M. Nighot n, P. Nighot n, T. Ma *, D. Malinowska*, G. Shull*, J. Cuppoletti*, A. Blikslager n 

Ed(s): A. Guerrero-Hernandez

MeSH headings : Animals; Biological Transport; CLC-2 Chloride Channels; Cell Count; Chloride Channels / genetics; Cytoplasmic Vesicles / metabolism; Digestion / genetics; Digestion / physiology; Fluorescent Antibody Technique; Gastric Acid / metabolism; H(+)-K(+)-Exchanging ATPase / biosynthesis; H(+)-K(+)-Exchanging ATPase / metabolism; Mice; Mice, Knockout; Microscopy, Electron; Parietal Cells, Gastric / metabolism; Parietal Cells, Gastric / ultrastructure; Pepsinogen A / metabolism
TL;DR: Genetic ablation of ClC-2 resulted in reduced gastric gland region, reduced parietal cell number, reduced H/K ATPase, reduced tubulovesicles and reduced stimulated acid secretion. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref, ORCID, NC State University Libraries
Added: August 6, 2018

2013 journal article

Chloride Channel ClC-2 is a Key Factor in the Development of DSS-induced Murine Colitis

Inflammatory Bowel Diseases, 19(13), 2867–2877.

By: P. Nighot n, K. Young n, M. Nighot n, M. Rawat *, E. Sung  n, N. Maharshak *, S. Plevy*, T. Ma *, A. Blikslager n 

Contributors: P. Nighot n, K. Young n, M. Nighot n, M. Rawat *, E. Sung  n, N. Maharshak *, S. Plevy*, T. Ma *, A. Blikslager n 

author keywords: inflammatory bowel disease; barrier function; tight junction
MeSH headings : Animals; Blotting, Western; CLC-2 Chloride Channels; Caco-2 Cells; Chloride Channels / physiology; Colitis / chemically induced; Colitis / metabolism; Colitis / pathology; Cytokines / metabolism; Dextran Sulfate / toxicity; Disease Models, Animal; Female; Humans; Immunoenzyme Techniques; Intestinal Mucosa / injuries; Intestinal Mucosa / metabolism; Intestinal Mucosa / pathology; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; RNA, Messenger / genetics; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; Tight Junctions / metabolism; Tight Junctions / pathology
TL;DR: ClC-2 plays a critical role in experimental colitis in that its absence increases disease activity, reduces barrier function and recovery, and perturbs TJs. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref, ORCID, NC State University Libraries
Added: August 6, 2018

2012 journal article

Characterization of turkey inducible nitric oxide synthase and identification of its expression in the intestinal epithelium following astrovirus infection

Comparative Immunology, Microbiology and Infectious Diseases, 35(1), 63–69.

By: R. Meyerhoff  n, P. Nighot n, R. Ali n, A. Blikslager n  & M. Koci n 

Contributors: R. Meyerhoff  n, P. Nighot n, R. Ali n, A. Blikslager n  & M. Koci n 

author keywords: Astrovirus; Enterocyte; Turkey; Inducible nitric oxide; Innate immunity
MeSH headings : Amino Acid Sequence; Animals; Astroviridae Infections / enzymology; Astroviridae Infections / veterinary; Astroviridae Infections / virology; Avastrovirus / physiology; Avian Proteins / genetics; Avian Proteins / metabolism; Bird Diseases / enzymology; Bird Diseases / virology; Enterocytes / enzymology; Enterocytes / pathology; Enterocytes / virology; Gene Expression; HEK293 Cells; Humans; Intestinal Mucosa / enzymology; Intestinal Mucosa / pathology; Intestinal Mucosa / virology; Lentivirus / genetics; Molecular Sequence Data; Nitric Oxide / biosynthesis; Nitric Oxide Synthase Type II / genetics; Nitric Oxide Synthase Type II / metabolism; Phylogeny; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Sequence Alignment; Transfection; Turkeys
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, Crossref, NC State University Libraries
Added: August 6, 2018

2011 journal article

Chloride channel ClC-2 modulates tight junction barrier function via intracellular trafficking of occludin

American Journal of Physiology-Cell Physiology, 302(1), C178–C187.

By: P. Nighot n & A. Blikslager n 

Contributors: P. Nighot n & A. Blikslager n 

author keywords: Caco-2; epithelium; intestine; permeability
MeSH headings : CLC-2 Chloride Channels; Caco-2 Cells; Caveolae / metabolism; Caveolae / physiology; Cell Communication / physiology; Chloride Channels / metabolism; Chloride Channels / physiology; Humans; Intracellular Fluid / metabolism; Intracellular Fluid / physiology; Membrane Proteins / metabolism; Membrane Proteins / physiology; Occludin; Protein Transport / physiology; Tight Junctions / metabolism; Tight Junctions / physiology
TL;DR: Data indicate that ClC-2 plays an important role in the modulation of tight junctions by influencing caveolar trafficking of the tight junction protein occludin. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref, ORCID, NC State University Libraries
Added: August 6, 2018

2010 journal article

Astrovirus infection induces sodium malabsorption and redistributes sodium hydrogen exchanger expression

Virology, 401(2), 146–154.

By: P. Nighot n, A. Moeser n, R. Ali n, A. Blikslager n  & M. Koci n 

Contributors: P. Nighot n, A. Moeser n, R. Ali n, A. Blikslager n  & M. Koci n 

author keywords: Astrovirus; Viral enteritis; Malabsorption diarrhea; Sodium/hydrogen exchanger; Intestinal electrophysiology; Turkey
MeSH headings : Actins / metabolism; Animals; Astroviridae Infections / pathology; Astroviridae Infections / veterinary; Avastrovirus / pathogenicity; Cell Membrane / chemistry; Cytoplasm / chemistry; Gene Expression; Intestinal Mucosa / pathology; Intestinal Mucosa / ultrastructure; Poultry Diseases / pathology; Poultry Diseases / virology; Sodium / metabolism; Sodium-Hydrogen Exchangers / analysis; Turkeys
TL;DR: Data suggest astrovirus infection induces sodium malabsorption, possibly through redistribution of specific sodium transporters, which results in the development of an osmotic diarrhoea in infants and the immunocompromised. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, ORCID, Crossref, NC State University Libraries
Added: August 6, 2018

2010 journal article

ClC-2 regulates mucosal barrier function associated with structural changes to the villus and epithelial tight junction

American Journal of Physiology-Gastrointestinal and Liver Physiology, 299(2), G449–G456.

By: P. Nighot n & A. Blikslager n 

Contributors: P. Nighot n & A. Blikslager n 

author keywords: myosin light chain kinase; chloride channel; mannitol flux
MeSH headings : Animals; CLC-2 Chloride Channels; Chloride Channels / antagonists & inhibitors; Chloride Channels / deficiency; Chloride Channels / metabolism; Chlorides / pharmacology; Electrophysiological Phenomena; Ileum / drug effects; Ileum / metabolism; Ileum / physiology; Ileum / ultrastructure; In Vitro Techniques; Intestinal Mucosa / drug effects; Intestinal Mucosa / metabolism; Intestinal Mucosa / physiology; Intestinal Mucosa / ultrastructure; Mannitol / pharmacokinetics; Mice; Mice, Knockout; Microscopy, Electron; Microscopy, Electron, Scanning; Microvilli / ultrastructure; Myosin-Light-Chain Kinase / metabolism; Permeability; Phenotype; Tight Junctions / ultrastructure; Zinc Compounds / pharmacology
TL;DR: It is indicated that ClC-2 reduces barrier function in normal mucosa, and the mechanisms underlying these differing roles are not entirely clear, although ultrastructural morphology of tight junctions and MLCK appear to be important to the function of ClC -2 innormal mucosa. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref, ORCID, NC State University Libraries
Added: August 6, 2018

2010 journal article

TGF-β–Activated Kinase 1 Signaling Maintains Intestinal Integrity by Preventing Accumulation of Reactive Oxygen Species in the Intestinal Epithelium

The Journal of Immunology, 185(8), 4729–4737.

By: R. Kajino-Sakamoto  n, E. Omori n, P. Nighot n, A. Blikslager n , K. Matsumoto * & J. Ninomiya-Tsuji n 

Contributors: R. Kajino-Sakamoto  n, E. Omori n, P. Nighot n, A. Blikslager n , K. Matsumoto * & J. Ninomiya-Tsuji n 

MeSH headings : Animals; Blotting, Western; Epithelium / enzymology; Epithelium / immunology; Gene Expression; Gene Expression Regulation / immunology; Immunity, Mucosal / physiology; Immunohistochemistry; Intestinal Mucosa / enzymology; Intestinal Mucosa / immunology; MAP Kinase Kinase Kinases / immunology; MAP Kinase Kinase Kinases / metabolism; Mice; Mice, Inbred C57BL; Mice, Transgenic; NF-E2-Related Factor 2 / immunology; NF-E2-Related Factor 2 / metabolism; Oxidative Stress / immunology; Reactive Oxygen Species / immunology; Reactive Oxygen Species / metabolism; Reverse Transcriptase Polymerase Chain Reaction; Signal Transduction
TL;DR: It is reported that TGF-β–activated kinase 1 (TAK1) is a key regulator of ROS in the intestinal epithelium and regulates ROS through transcription factor NF-E2–related factor 2, which is important for intestinal epithelial integrity. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref, ORCID, NC State University Libraries
Added: August 6, 2018

2009 journal article

Epigenetic Reprogramming by Somatic Cell Nuclear Transfer in Primates

STEM CELLS, 27(6), 1255–1264.

By: M. Sparman*, V. Dighe *, H. Sritanaudomchai *, H. Ma *, C. Ramsey*, D. Pedersen*, L. Clepper*, P. Nighot n ...

author keywords: Reprogramming; Nuclear transfer; Embryonic stem cells; Primates
MeSH headings : Animals; Cell Differentiation; Cellular Reprogramming / genetics; Embryonic Stem Cells / cytology; Epigenesis, Genetic; Female; Fibroblasts / cytology; Gene Expression; Macaca mulatta; Nuclear Transfer Techniques; Oocytes / cytology; Pluripotent Stem Cells / cytology; Reverse Transcriptase Polymerase Chain Reaction
TL;DR: Nearly threefold higher blastocyst development and embryonic stem (ES) cell derivation rates with different nuclear donor cells are demonstrated. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2008 journal article

ClC-2 is required for rapid restoration of epithelial tight junctions in ischemic-injured murine jejunum

Experimental Cell Research, 315(1), 110–118.

By: P. Nighot n, A. Moeser n, K. Ryan n, T. Ghashghaei n & A. Blikslager n 

Contributors: P. Nighot n, A. Moeser n, K. Ryan n, T. Ghashghaei n & A. Blikslager n 

author keywords: Tight junction; Intestinal permeability; Ischemia; ClC-2
MeSH headings : Animals; CLC-2 Chloride Channels; Cell Membrane / metabolism; Chloride Channels / deficiency; Chloride Channels / metabolism; Epithelium / metabolism; Epithelium / pathology; Epithelium / ultrastructure; Fluorescent Antibody Technique; Immunoprecipitation; Ischemia / metabolism; Ischemia / physiopathology; Jejunum / blood supply; Jejunum / pathology; Jejunum / ultrastructure; Mannitol / metabolism; Membrane Proteins / metabolism; Mice; Mice, Knockout; Occludin; Protein Transport; Tight Junctions / metabolism; Tight Junctions / ultrastructure; Wound Healing
TL;DR: A pivotal role is demonstrated in recovery of the intestinal epithelium barrier by anchoring assembly of tight junctions following ischemic injury in ClC-2(-/-) mice. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref, ORCID, NC State University Libraries
Added: August 6, 2018

2008 journal article

Comparison of the chloride channel activator lubiprostone and the oral laxative Polyethylene Glycol 3350 on mucosal barrier repair in ischemic-injured porcine intestine

World Journal of Gastroenterology, 14(39), 6012.

By: A. Moeser n, P. Nighot*, B. Roerig*, R. Ueno* & A. Blikslager n 

Contributors: A. Moeser n, P. Nighot*, B. Roerig*, R. Ueno* & A. Blikslager n 

author keywords: Intestinal ischemia; Barrier function; Permeability; Laxative; Polyethylene Glycol
MeSH headings : Alprostadil / analogs & derivatives; Alprostadil / pharmacology; Animals; Cell Membrane Permeability / drug effects; Cell Membrane Permeability / physiology; Chloride Channel Agonists; Disease Models, Animal; Epithelial Cells / drug effects; Epithelial Cells / physiology; Female; Ileum / blood supply; Intestinal Mucosa / drug effects; Intestinal Mucosa / pathology; Intestinal Mucosa / physiopathology; Inulin / metabolism; Laxatives / pharmacology; Lubiprostone; Male; Mannitol / metabolism; Polyethylene Glycols / pharmacology; Recovery of Function / drug effects; Reperfusion Injury / pathology; Reperfusion Injury / physiopathology; Swine
TL;DR: Results suggest that, unlike osmotic laxatives, lubiprostone stimulates repair of the injured intestinal barrier, whereas the PEG laxative had deleterious effects on mucosal repair. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref, ORCID, NC State University Libraries
Added: August 6, 2018

2008 journal article

Mice lacking the Na+/H+ exchanger 2 have impaired recovery of intestinal barrier function

American Journal of Physiology-Gastrointestinal and Liver Physiology, 295(4), G791–G797.

By: A. Moeser n, P. Nighot n, K. Ryan n, J. Simpson*, L. Clarke * & A. Blikslager n 

Contributors: A. Moeser n, P. Nighot n, K. Ryan n, J. Simpson*, L. Clarke * & A. Blikslager n 

author keywords: tight junction; intestinal permeability; ischemia; Na(+)/H(+) exchange
MeSH headings : Animals; Claudin-1; Intestinal Mucosa / physiopathology; Intestine, Small / blood supply; Ischemia / physiopathology; Mannitol / metabolism; Membrane Proteins / metabolism; Mice; Mice, Knockout; Occludin; Permeability; Phosphorylation; Sodium-Hydrogen Exchangers / genetics; Sodium-Hydrogen Exchangers / physiology; Tight Junctions / metabolism; Tight Junctions / ultrastructure
TL;DR: Recovery of barrier function was assessed in wild-type (NHE2(+/+)) and NHE2(-/-) mice in vivo and in vitro and pharmacological inhibition of N HE2 in postischemic tissues from wild- type mice also resulted in significant reductions in TER. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: August 6, 2018

2007 journal article

Gastrointestinal dysfunction induced by early weaning is attenuated by delayed weaning and mast cell blockade in pigs

American Journal of Physiology-Gastrointestinal and Liver Physiology, 293(2), G413–G421.

By: A. Moeser n, K. Ryan n, P. Nighot* & A. Blikslager n 

Contributors: A. Moeser n, K. Ryan n, P. Nighot* & A. Blikslager n 

author keywords: stress; barrier function; corticotropin releasing factor; tryptase
MeSH headings : Aging / metabolism; Animals; Cell Degranulation / drug effects; Colon, Ascending / drug effects; Colon, Ascending / metabolism; Corticotropin-Releasing Hormone / blood; Cromolyn Sodium / pharmacology; Cromolyn Sodium / therapeutic use; Electric Impedance; Gastrointestinal Agents / pharmacology; Gastrointestinal Agents / therapeutic use; Gastrointestinal Diseases / enzymology; Gastrointestinal Diseases / metabolism; Gastrointestinal Diseases / prevention & control; Gastrointestinal Diseases / psychology; Hydrocortisone / blood; Intestinal Mucosa / drug effects; Intestinal Mucosa / metabolism; Intestinal Secretions / metabolism; Jejunum / drug effects; Jejunum / metabolism; Mannitol / metabolism; Mast Cells / drug effects; Mast Cells / enzymology; Mast Cells / metabolism; Permeability; Receptors, Corticotropin-Releasing Hormone / metabolism; Stress, Psychological / complications; Stress, Psychological / enzymology; Stress, Psychological / metabolism; Swine; Time Factors; Tryptases / metabolism; Up-Regulation; Weaning
TL;DR: The results indicate that early-weaning stress induces mucosal dysfunction mediated by intestinal mast cell activation and can be prevented by delaying weaning. (via Semantic Scholar)
Sources: Web Of Science, Crossref, ORCID, NC State University Libraries
Added: August 6, 2018

2006 journal article

Prostaglandin-mediated inhibition of Na+/H+ exchanger isoform 2 stimulates recovery of barrier function in ischemia-injured intestine

American Journal of Physiology-Gastrointestinal and Liver Physiology, 291(5), G885–G894.

By: A. Moeser n, P. Nighot*, K. Ryan*, J. Wooten* & A. Blikslager* 

Contributors: A. Moeser n, P. Nighot*, K. Ryan*, J. Wooten* & A. Blikslager* 

author keywords: tight junction; restitution; Na+/H+ exchange
MeSH headings : Animals; Blotting, Western; Diffusion Chambers, Culture; Electric Impedance; Electrophoresis, Polyacrylamide Gel; Female; Guanidines / pharmacology; Ileum / blood supply; Ileum / metabolism; Immunohistochemistry; Immunoprecipitation; Intestinal Absorption / drug effects; Intestinal Absorption / physiology; Intestinal Mucosa / blood supply; Intestinal Mucosa / metabolism; Intestines / blood supply; Intestines / pathology; Ischemia / pathology; Male; Methacrylates / pharmacology; Prostaglandins / pharmacology; Regional Blood Flow / drug effects; Regional Blood Flow / physiology; Sodium / metabolism; Sodium-Hydrogen Exchangers / antagonists & inhibitors; Sulfones / pharmacology; Swine
TL;DR: Selective inhibition of NHE2, and not NHE3, induces recovery of barrier function in the ischemia-injured intestine. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref, ORCID, NC State University Libraries
Added: August 6, 2018

2006 journal article

Recovery of mucosal barrier function in ischemic porcine ileum and colon is stimulated by a novel agonist of the ClC-2 chloride channel, lubiprostone

American Journal of Physiology-Gastrointestinal and Liver Physiology, 292(2), G647–G656.

By: A. Moeser n, P. Nighot*, K. Engelke*, R. Ueno* & A. Blikslager n 

Contributors: A. Moeser n, P. Nighot*, K. Engelke*, R. Ueno* & A. Blikslager n 

author keywords: ischemia; type 2 chloride channels; repair
MeSH headings : Alprostadil / analogs & derivatives; Alprostadil / pharmacology; Alprostadil / therapeutic use; Animals; Bumetanide / pharmacology; CLC-2 Chloride Channels; Chloride Channel Agonists; Chloride Channels / antagonists & inhibitors; Chlorides / pharmacology; Colon / blood supply; Colon / drug effects; Colon / physiopathology; Cystic Fibrosis Transmembrane Conductance Regulator / antagonists & inhibitors; Dinoprostone / pharmacology; Electric Impedance; Electrophysiology; Fatty Acids / pharmacology; Fatty Acids / therapeutic use; Female; Ileum / blood supply; Ileum / drug effects; Ileum / physiopathology; Indomethacin / pharmacology; Intestinal Mucosa / drug effects; Intestinal Mucosa / pathology; Intestinal Mucosa / physiopathology; Ischemia / metabolism; Ischemia / physiopathology; Lubiprostone; Male; Mannitol / metabolism; Membrane Proteins / metabolism; Occludin; Permeability; Recovery of Function / drug effects; Sus scrofa; Urea / analogs & derivatives; Urea / pharmacology; Zinc Compounds / pharmacology
TL;DR: Prostones such as lubiprostone may provide a selective and novel pharmacological mechanism of accelerating recovery of acutely injured intestine compared with the nonselective action of prostaglandins such as PGE(2). (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref, ORCID, NC State University Libraries
Added: August 6, 2018

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