@article{rouse_haslauer_loboa_monteiro-riviere_2008, title={Cyclic tensile strain increases interactions between human epidermal keratinocytes and quantum dot nanoparticles}, volume={22}, ISSN={["0887-2333"]}, url={http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000254694500024&KeyUID=WOS:000254694500024}, DOI={10.1016/j.tiv.2007.10.010}, abstractNote={The effects of quantum dots (QD) on cell viability have gained increasing interest due to many recent developments utilizing QD for pharmaceutical and biomedical applications. The potential use of QD nanoparticles as diagnostic, imaging, and drug delivery agents has raised questions about their potential for cytotoxicity. The objective of this study was to investigate the effects of applied strain on QD uptake by human epidermal keratinocytes (HEK). It was hypothesized that introduction of a 10% average strain to cell cultures would increase QD uptake. HEK were seeded at a density of 150,000 cells/mL on collagen-coated Flexcell culture plates (Flexcell Intl.). QD were introduced at a concentration of 3 nM and a 10% average strain was applied to the cells. After 4 h of cyclic strain, the cells were examined for cell viability, QD uptake, and cytokine production. The results indicate that addition of strain results in an increase in cytokine production and QD uptake, resulting in irritation and a negative impact on cell viability. Application of physiological load conditions can increase cell membrane permeability, thereby increasing the concentration of QD nanoparticles in cells.}, number={2}, journal={TOXICOLOGY IN VITRO}, author={Rouse, Jillian G. and Haslauer, Carla M. and Loboa, Elizabeth G. and Monteiro-Riviere, Nancy A.}, year={2008}, month={Mar}, pages={491–497} }
 @article{walker_monteiro-riviere_rouse_adrian t. o'neill_2007, title={A linear dilution microfluidic device for cytotoxicity assays}, volume={7}, ISSN={["1473-0189"]}, url={http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000244616300011&KeyUID=WOS:000244616300011}, DOI={10.1039/b608990a}, abstractNote={A two-layer polymer microfluidic device is presented which creates nine linear dilutions from two input fluid streams mixed in varying volumetric proportions. The linearity of the nine dilutions is conserved when the flow rate is held constant at 1.0 microl min(-1) (R(2) = 0.9995) and when it is varied from 0.5-16 microl min(-1) (R(2) = 0.9998). An analytical expression is presented for designing microfluidic devices with arbitrary numbers of linear dilutions. To demonstrate the efficacy of this device, primary human epidermal keratinocytes (HEK) were stained with nine dilutions of calcein, resulting in a linear spread of fluorescent intensities (R(2) = 0.94). The operating principles of the device can be scaled up to incorporate any number of linear dilutions. This scalability, coupled with an intrinsic ability to create linear dilutions under a variety of operating conditions, makes the device applicable to high throughput screening applications such as combinatorial chemistry or cytotoxicity assays.}, number={2}, journal={LAB ON A CHIP}, author={Walker, Glenn M. and Monteiro-Riviere, Nancy and Rouse, Jillian and Adrian T. O'Neill}, year={2007}, pages={226–232} }
 @article{rouse_yang_ryman-rasmussen_barron_monteiro-riviere_2007, title={Effects of mechanical flexion on the penetration of fullerene amino acid-derivatized peptide nanoparticles through skin}, volume={7}, ISSN={["1530-6992"]}, url={http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000243381300027&KeyUID=WOS:000243381300027}, DOI={10.1021/nl062464m}, abstractNote={Dermatomed porcine skin was fixed to a flexing device and topically dosed with 33.5 mg.mL-1 of an aqueous solution of a fullerene-substituted phenylalanine (Baa) derivative of a nuclear localization peptide sequence (Baa-Lys(FITC)-NLS). Skin was flexed for 60 or 90 min or left unflexed (control). Confocal microscopy depicted dermal penetration of the nanoparticles at 8 h in skin flexed for 60 and 90 min, whereas Baa-Lys(FITC)-NLS did not penetrate into the dermis of unflexed skin until 24 h. TEM analysis revealed fullerene-peptide localization within the intercellular spaces of the stratum granulosum.}, number={1}, journal={NANO LETTERS}, author={Rouse, Jillian G. and Yang, Jianzhong and Ryman-Rasmussen, Jessica P. and Barron, Andrew R. and Monteiro-Riviere, Nancy A.}, year={2007}, month={Jan}, pages={155–160} }
 @article{rouse_yang_barron_monteiro-riviere_2006, title={Fullerene-based amino acid nanoparticle interactions with human epidermal keratinocytes}, volume={20}, ISSN={["0887-2333"]}, url={http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000242136100006&KeyUID=WOS:000242136100006}, DOI={10.1016/j.tiv.2006.04.004}, abstractNote={The functionalization of C(60) with such complexes as amino acids has the potential to provide greater interaction between the fullerene and the biological environment yielding potential new medical and pharmacological applications. Although scientific research in the past decade has revealed much about the chemical and physical properties of C(60), the biological activities of this compound and its derivatives are still relatively unclear. In an attempt to understand the biological activity of functionalized C(60), human epidermal keratinocytes (HEK) were exposed to fullerene-based amino acid (Baa) solutions ranging in concentrations of 0.4-0.00004 mg/mL in a humidified 5% CO(2) atmosphere at 37 degrees C. MTT cell viability after 48 h significantly decreased (p<0.05) for concentrations of 0.4 and 0.04 mg/mL. In an additional study, human cytokines IL-6, IL-8, TNF-alpha, IL-1beta, and IL-10 were assessed for concentrations ranging from 0.4-0.004 mg/mL. Media was harvested at 1, 4, 8, 12, 24 and 48 h for cytokine analysis. IL-8 concentrations for the 0.04 mg/mL treatment were significantly greater (p<0.05) than all other concentrations at 8, 12, 24, and 48 h. IL-6 and IL-1beta activities were greater at the 24h and 48 h for 0.4 and 0.04 mg/mL. No significant TNF-alpha or IL-10 activity existed at any time points for any of the concentrations. These results indicate that concentrations lower than 0.04 mg/mL initiate less cytokine activity and maintain cell viability. In HEK, Baa concentrations of 0.4 and 0.04 mg/mL decrease cell viability and initiate a pro-inflammatory response.}, number={8}, journal={TOXICOLOGY IN VITRO}, author={Rouse, Jillian G. and Yang, Jianzhong and Barron, Andrew R. and Monteiro-Riviere, Nancy A.}, year={2006}, month={Dec}, pages={1313–1320} }