Edwin Bruce Collins

Works (6)

Updated: July 5th, 2023 16:04

2016 journal article

Effect of Plant Species, Fertilizer Acidity/Basicity, and Fertilizer Concentration on pH of Soilless Root Substrate

HORTSCIENCE, 51(12), 1596–1601.

By: K. Jeong n, P. Nelson n, C. Niedziela  n & D. Dickey n

author keywords: alkalinity; electrical conductivity; root media
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 journal article

Production of ELOVL4 transgenic pigs: a large animal model for Stargardt-like macular degeneration

British Journal of Ophthalmology, 95(12), 1749–1754.

By: J. Sommer n, J. Estrada n, E. Collins n, M. Bedell *, C. Alexander n, Z. Yang *, G. Hughes*, B. Mir n ...

MeSH headings : Animals; Animals, Genetically Modified; Disease Models, Animal; Electroretinography; Eye Proteins / biosynthesis; Eye Proteins / genetics; Gene Deletion; Immunohistochemistry; Macular Degeneration / metabolism; Macular Degeneration / pathology; Macular Degeneration / physiopathology; Membrane Proteins / biosynthesis; Membrane Proteins / genetics; Microscopy, Fluorescence; Mutation; Retina / metabolism; Retina / pathology; Retina / physiopathology; Swine
TL;DR: These transgenic pigs provide unique animal models for examining macular degeneration and STGD3 pathogenesis and direct fluorescence microscopy indicated that the Y270terEYFP protein is expressed in photoreceptors and mislocalised within the cell. (via Semantic Scholar)
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Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018

2011 journal article

Transgenic Stra8-EYFP pigs: a model for developing male germ cell technologies

TRANSGENIC RESEARCH, 21(2), 383–392.

By: J. Sommer n, L. Jackson n, S. Simpson n, E. Collins n, J. Piedrahita n  & R. Petters n

author keywords: Pig; Transgenic; Stra8; Spermatogonia; Marker gene
MeSH headings : Adaptor Proteins, Signal Transducing; Animals; Animals, Genetically Modified / genetics; Animals, Genetically Modified / metabolism; Blotting, Western; Cells, Cultured; Cloning, Molecular; Fibroblasts / cytology; Fibroblasts / drug effects; Fibroblasts / metabolism; Gene Expression Regulation, Developmental; Genes, Reporter; Germ Cells / cytology; Germ Cells / metabolism; Homeodomain Proteins / genetics; Homeodomain Proteins / metabolism; Immunohistochemistry; Luminescent Proteins / genetics; Luminescent Proteins / metabolism; Male; Mice; Microscopy, Fluorescence / methods; Mitochondria / genetics; Mitochondria / metabolism; Nuclear Transfer Techniques; Plasmids / genetics; Plasmids / metabolism; Promoter Regions, Genetic; Proteins / genetics; Proteins / metabolism; Swine; Testis / cytology; Testis / metabolism; Transfection; Transgenes; Tretinoin / pharmacology
TL;DR: Transgenic pigs expressing mitochondrial localized enhanced yellow fluorescent protein (EYFP-mito) under control of the germ cell specific Stimulated by Retinoic Acid 8 (Stra8) promoter indicates that these pigs will be useful by providing labelled cells for use in such technologies such as germ cell transplantation and in vitro spermatogenic studies. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2006 journal article

Synchronization and superovulation of mature cycling gilts for the collection of pronuclear stage embryos

ANIMAL REPRODUCTION SCIENCE, 100(3-4), 402–410.

By: J. Sommer n, E. Collins n, J. Estrada n & R. Petters n

author keywords: gilt; superovulation; estrous synchronization; pronuclear stage embryo; PG600 (R); prostaglandin F-2 alpha; transgenic
MeSH headings : Animals; Chorionic Gonadotropin / pharmacology; Dinoprost / analogs & derivatives; Dinoprost / pharmacology; Embryo, Mammalian; Estrous Cycle; Estrus Synchronization / methods; Female; Superovulation / drug effects; Superovulation / physiology; Swine / physiology
TL;DR: An efficient protocol to synchronize and superovulate mature pigs for the collection of pronuclear stage embryos suitable for DNA microinjection and Pigs 10-16 days after the beginning of standing estrus have been successfully synchronized into estrus using this protocol. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2002 journal article

Conservation and regeneration of transgenic lines of swine by semen cryopreservation and artificial insemination

Lab Animal, 31(1), 25–31.

By: J. Sommer, E. Collins, T. Neiding, K. Rozeboom, F. Wong & R. Petters

Source: NC State University Libraries
Added: August 6, 2018

1997 journal article

Genetically engineered large animal model for studying cone photoreceptor survival and degeneration in retinitis pigmentosa

NATURE BIOTECHNOLOGY, 15(10), 965–970.

By: R. Petters n, C. Alexander n, K. Wells n, E. Collins n, . Sommer n, M. Blanton n, G. Rojas n, Y. Hao* ...

author keywords: transgenic swine; retinal degeneration; rhodopsin mutation; night blindness; eye disease
MeSH headings : Animals; Animals, Genetically Modified; Blotting, Southern; Disease Models, Animal; Electroretinography; Embryo Transfer; Gene Expression Regulation / genetics; Genetic Engineering; Microscopy, Electron; Molecular Sequence Data; Phenotype; Polymerase Chain Reaction; Retina / pathology; Retina / physiopathology; Retina / ultrastructure; Retinal Cone Photoreceptor Cells / physiopathology; Retinal Cone Photoreceptor Cells / ultrastructure; Retinal Degeneration / physiopathology; Retinitis Pigmentosa / genetics; Retinitis Pigmentosa / physiopathology; Rhodopsin / chemistry; Rhodopsin / genetics; Swine / embryology; Swine / genetics; Transgenes
TL;DR: These transgenic pigs that express a mutated rhodopsin gene will provide a large animal model for study of the protracted phase of cone degeneration found in RP and for preclinical treatment trials. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

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