@article{lourenco_randall_seiler_lunn_2015, title={Abdominal ultrasonographic findings in acromegalic cats}, volume={17}, ISSN={["1532-2750"]}, DOI={10.1177/1098612x14556847}, abstractNote={Objectives Acromegaly is increasingly recognized as a cause of insulin resistance in cats with diabetes mellitus (DM). The objective of this study was to determine if ultrasonographic changes in selected abdominal organs of acromegalic cats could be used to raise the index of suspicion for this condition. Methods In this retrospective case-control study, medical records of cats presenting to North Carolina State University or Colorado State University from January 2002 to October 2012 were reviewed. Cats were included in the acromegaly group if they had insulin-resistant DM with increased serum insulin-like growth factor (IGF-1) concentrations and had an abdominal ultrasound examination performed with report available. A control group included age-matched cats that had abdominal ultrasound examination performed for investigation of disease unlikely to involve the kidneys, adrenal glands, pancreas or liver. Results Twenty-four cats were included in each group. IGF-1 concentrations in the acromegaly group ranged from >148 to 638 nmol/l. When compared with age-matched controls, cats with acromegaly demonstrated significantly increased median left and right kidney length, significantly increased median left and right adrenal gland thickness, and significantly increased median pancreatic thickness. Hepatomegaly and bilateral adrenomegaly were reported in 63% and 53% of acromegalic cats, respectively, and in none of the controls. Pancreatic abnormalities were described in 88% of the acromegalic cats and 8% of the controls. Conclusions and relevance These findings indicate that compared with non-acromegalic cats, age-matched acromegalic patients have measurably larger kidneys, adrenal glands and pancreas. Diagnostic testing for acromegaly should be considered in poorly regulated diabetic cats exhibiting organomegaly on abdominal ultrasound examination.}, number={8}, journal={JOURNAL OF FELINE MEDICINE AND SURGERY}, author={Lourenco, Bianca N. and Randall, Elissa and Seiler, Gabriela and Lunn, Katharine F.}, year={2015}, month={Aug}, pages={698–703} }
 @article{song_lu_chen_lourenco_chiang_2006, title={Genetic transformation of Populus trichocarpa genotype Nisqually-1: A functional genomic tool for woody plants}, volume={47}, ISSN={["1471-9053"]}, DOI={10.1093/pcp/pcl018}, abstractNote={We report here the Agrobacterium-mediated genetic transformation of Nisqually-1, a Populus trichocarpa genotype whose genome was recently sequenced. Several systems were established. Internodal stem segments from vigorously growing greenhouse plants are the explants most amenable to transformation. For the most efficient system, approximately 40% of the stem segments infected with pBI121-containing Agrobacterium tumefaciens C58 produced transgenic calli, as confirmed by beta-glucuronidase (GUS) staining. The regeneration efficiency of independent transgenic plants was approximately 13%, as revealed by genomic Southern analysis. Some transgenic plants were produced in as little as 5 months after co-cultivation. This system may help to facilitate studies of gene functions in tree growth and development at a genome level.}, number={11}, journal={PLANT AND CELL PHYSIOLOGY}, author={Song, Jingyuan and Lu, Shanfa and Chen, Zenn-Zong and Lourenco, Rodrigo and Chiang, Vincent L.}, year={2006}, month={Nov}, pages={1582–1589} }