@article{adrian t. o'neill_monteiro-riviere_walker_2009, title={Microfabricated curtains for controlled cell seeding in high throughput microfluidic systems}, volume={9}, ISSN={["1473-0189"]}, url={http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000266616000016&KeyUID=WOS:000266616000016}, DOI={10.1039/b819622b}, abstractNote={A microfabricated cell curtain is presented that facilitates cellular assays. The cell curtain is defined as a poly(dimethylsiloxane) (PDMS) wall that extends from the ceiling of a cell culture microchamber to within microns of the chamber floor. Curtain use is demonstrated by observing monolayer human epidermal keratinocyte (HEK) colonies for 48 h longer than possible with non-curtained microfluidic chambers. The curtains were further characterized by integrating them into a 96 chamber high throughput microfluidic cell culture device. As proof of concept, this device was used to assay a range of ethanol dilutions spanning 0-22% in cell culture medium. Cells exposed to 12% ethanol or less for 30 min would recover to 85% viability at 24 h, while cells exposed to higher concentrations had viabilities below 10%. The data also showed that cells exposed to 6% ethanol or less grew in population size, 8% ethanol exposure stunted growth, and higher concentrations led to population loss. Curtain use permitted high initial cell seeding densities and increased the amount of time cells can be cultured compared to multi-well plates.}, number={12}, journal={LAB ON A CHIP}, author={Adrian T. O'Neill and Monteiro-Riviere, Nancy A. and Walker, Glenn M.}, year={2009}, pages={1756–1762} }
 @article{adrian t. o'neill_monteiro-riviere_walker_2008, title={Characterization of microfluidic human epidermal keratinocyte culture}, volume={56}, ISSN={["0920-9069"]}, url={http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000257370900007&KeyUID=WOS:000257370900007}, DOI={10.1007/s10616-008-9149-9}, abstractNote={Human epidermal keratinocytes (HEK) are skin cells of primary importance in maintaining the body's defensive barrier and are used in vitro to assess the irritation potential and toxicity of chemical compounds. Microfluidic systems hold promise for high throughput irritant and toxicity assays, but HEK growth kinetics have yet to be characterized within microscale culture chambers. This research demonstrates HEK patterning on microscale patches of Type I collagen within microfluidic channels and maintenance of these cells under constant medium perfusion for 72 h. HEK were shown to maintain 93.0%-99.6% viability at 72 h under medium perfusion ranging from 0.025-0.4 mul min(-1). HEK maintained this viability while approximately 100% confluent-a level not possible in 96 well plates. Microscale HEK cultures offer the ability to precisely examine the morphology, behavior and viability of individual cells which may open the door to new discoveries in toxicological screening methods and wound healing techniques.}, number={3}, journal={CYTOTECHNOLOGY}, author={Adrian T. O'Neill and Monteiro-Riviere, Nancy A. and Walker, Glenn M.}, year={2008}, month={Mar}, pages={197–207} }
 @article{walker_monteiro-riviere_rouse_adrian t. o'neill_2007, title={A linear dilution microfluidic device for cytotoxicity assays}, volume={7}, ISSN={["1473-0189"]}, url={http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000244616300011&KeyUID=WOS:000244616300011}, DOI={10.1039/b608990a}, abstractNote={A two-layer polymer microfluidic device is presented which creates nine linear dilutions from two input fluid streams mixed in varying volumetric proportions. The linearity of the nine dilutions is conserved when the flow rate is held constant at 1.0 microl min(-1) (R(2) = 0.9995) and when it is varied from 0.5-16 microl min(-1) (R(2) = 0.9998). An analytical expression is presented for designing microfluidic devices with arbitrary numbers of linear dilutions. To demonstrate the efficacy of this device, primary human epidermal keratinocytes (HEK) were stained with nine dilutions of calcein, resulting in a linear spread of fluorescent intensities (R(2) = 0.94). The operating principles of the device can be scaled up to incorporate any number of linear dilutions. This scalability, coupled with an intrinsic ability to create linear dilutions under a variety of operating conditions, makes the device applicable to high throughput screening applications such as combinatorial chemistry or cytotoxicity assays.}, number={2}, journal={LAB ON A CHIP}, author={Walker, Glenn M. and Monteiro-Riviere, Nancy and Rouse, Jillian and Adrian T. O'Neill}, year={2007}, pages={226–232} }
 @inbook{o'neill_monteiro-riviere_walker_2006, title={A serial dilution microfluidic device for cytotoxicity assays}, booktitle={28th annual International Conference of the IEEE Engineering in Medicine and Biology Society}, publisher={Piscataway, NJ: IEEE}, author={O'Neill, A. T. and Monteiro-Riviere, N. A. and Walker, G. M.}, year={2006}, pages={2836–2839} }