@misc{velev_gupta_2009, title={Materials Fabricated by Micro- and Nanoparticle Assembly - The Challenging Path from Science to Engineering}, volume={21}, ISSN={["0935-9648"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-66249132369&partnerID=MN8TOARS}, DOI={10.1002/adma.200801837}, abstractNote={AbstractWe classify the strategies for colloidal assembly and review the diverse potential applications of micro‐ and nanoparticle structures in materials and device prototypes. The useful properties of the particle assemblies, such as high surface‐to‐volume ratio, periodicity at mesoscale, large packing density, and long‐range ordering, can be harnessed in optical, electronic, and biosensing devices. We discuss the present and future trends in the colloidal‐ assembly field, focusing on the challenges of developing fabrication procedures that are rapid and efficiently controlled. We speculate on how the issues of scalability, control, and precision could be addressed, and how the functionality of the assemblies can be increased to better match the needs of technology.}, number={19}, journal={ADVANCED MATERIALS}, author={Velev, Orlin D. and Gupta, Shalini}, year={2009}, month={May}, pages={1897–1905} }
 @article{gupta_huda_kilpatrick_velev_2007, title={Characterization and optimization of gold nanoparticle-based silver-enhanced immunoassays}, volume={79}, ISSN={["1520-6882"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-34249093580&partnerID=MN8TOARS}, DOI={10.1021/ac062341m}, abstractNote={Silver-enhanced nanoparticle-labeled immunoassays provide a simple, low-cost, and effective way of detecting antigens in dilute solutions. The physical mechanisms behind their operation, however, have not been fully investigated. We present a semiquantitative approach for optimizing sandwich nanoparticle immunoassays using an adsorption-controlled kinetic model. Primary antibodies were immobilized on a solid substrate to bind the target antigens in solution. An optical signal was measured by secondary labeling of antigens with gold nanoparticles and their enhancement by silver nucleation. The opacity of the silver-enhanced spots was quantified by densitometry. The selectivity of the sandwich immunoassays was adequately high, and antigen concentrations as low as 0.1 microg cm(-3) (4 ng total) were detected reproducibly. The role of mass transfer was investigated, and a model was developed to optimize the performance of immunoassays by correlating the opacities of silver spots to the concentration and incubation times of antigens and gold nanoparticles. The results could allow the development of more rapid and reliable nanoparticle immunoassays.}, number={10}, journal={ANALYTICAL CHEMISTRY}, author={Gupta, Shalini and Huda, Sabil and Kilpatrick, Peter K. and Velev, Orlin D.}, year={2007}, month={May}, pages={3810–3820} }