Works (1)
Updated: July 5th, 2023 15:55
2007 journal article
Membrane proteomic analysis of Arabidopsis thaliana using alternative solubilization techniques
JOURNAL OF PROTEOME RESEARCH, 6(5), 1933–1950.
author keywords: arabidopsis; proteomics; liquid chromatography; mass spectrometry; membrane proteins; phosphorylation; leucine-rich repeat receptor-like kinases
MeSH headings : Amino Acid Sequence; Arabidopsis / chemistry; Arabidopsis / cytology; Arabidopsis Proteins / analysis; Arabidopsis Proteins / genetics; Arabidopsis Proteins / isolation & purification; Bacteriorhodopsins / analysis; Bacteriorhodopsins / genetics; Bacteriorhodopsins / isolation & purification; Cetomacrogol / chemistry; Chromatography, Liquid / methods; Cytochromes c / analysis; Cytochromes c / genetics; Cytochromes c / isolation & purification; Membrane Proteins / analysis; Membrane Proteins / genetics; Membrane Proteins / isolation & purification; Methanol / chemistry; Molecular Sequence Data; Peptides / analysis; Peptides / chemistry; Peptides / genetics; Proteome / analysis; Solubility; Surface-Active Agents / chemistry; Tandem Mass Spectrometry / methods
TL;DR:
Overall, the data show that methanol is a better alternative for identifying a wider range of membrane proteins than the nonionic detergent Brij-58.
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Added: August 6, 2018
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