Sara L. Milam

Works (3)

Updated: July 5th, 2023 15:55

2009 journal article

Folding and assembly kinetics of procaspase-3

PROTEIN SCIENCE, 18(12), 2500–2517.

By: S. Milam n & A. Clark n

author keywords: apoptosis; dimer assembly; kinetic trap; burst-phase kinetics
MeSH headings : Caspase 3 / chemistry; Caspase 3 / metabolism; Circular Dichroism; Dimerization; Enzyme Activation; Models, Molecular; Protein Conformation; Protein Folding; Spectrometry, Fluorescence
TL;DR: The folding and assembly of procaspase‐3 is examined by fluorescence emission, circular dichroism, differential quenching by acrylamide, anisotropy, and enzyme activity assays and suggest that slow dimerization results from the lack of stabilizing native contacts in the initial encounter complex. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2009 review

Practical approaches to protein folding and assembly: Spectroscopic strategies in thermodynamics and kinetics

[Review of ]. Methods in enzymology: biothermodynamics,vol 455, part a, 455, 1–39.

By: J. Walters, S. Milam & A. Clark

Source: NC State University Libraries
Added: August 6, 2018

2007 journal article

Rapid folding and unfolding of Apaf-1 CARD

JOURNAL OF MOLECULAR BIOLOGY, 369(1), 290–304.

By: S. Milam n, N. Nicely n, B. Feeney n, C. Mattos n & A. Clark n

author keywords: caspase recruitment domain; protein folding; folding kinetics; parallel folding channels; sequential mixing stopped-flow
MeSH headings : Apoptotic Protease-Activating Factor 1 / chemistry; Apoptotic Protease-Activating Factor 1 / metabolism; Crystallography, X-Ray; Fluorescence; Kinetics; Models, Biological; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Thermodynamics
TL;DR: Examination of the equilibrium and kinetic folding of the CARD of Apaf-1 (apoptotic protease activating factor 1), which consists of 97 amino acid residues, at pH 6 and pH 8 showed that the native ensemble is formed rapidly upon refolding, in contrast to other CARDs in which folding appears to be dominated by formation of kinetic traps. (via Semantic Scholar)
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Added: August 6, 2018

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