Reduction of protease inhibitor activity by expression of a mutant Bowman-Birk gene in soybean seed
Livingstone, D., Beilinson, V., Kalyaeva, M., Schmidt, M. A., Herman, E. M., & Nielsen, N. C. (2007, April 11). Plant Molecular Biology.
author keywords: bowman-birk; anti-nutritional; kunitz; transgenic; soybean; glycine max
MeSH headings : Amino Acid Sequence; Base Sequence; Molecular Sequence Data; Mutation; RNA, Messenger / metabolism; Seeds / genetics; Seeds / metabolism; Sequence Alignment; Soybeans / embryology; Soybeans / genetics; Soybeans / metabolism; Trypsin Inhibitor, Bowman-Birk Soybean / chemistry; Trypsin Inhibitor, Bowman-Birk Soybean / genetics; Trypsin Inhibitor, Bowman-Birk Soybean / metabolism; Trypsin Inhibitor, Kunitz Soybean / genetics; Trypsin Inhibitor, Kunitz Soybean / metabolism; Trypsin Inhibitors / genetics; Trypsin Inhibitors / metabolism
topics (OpenAlex): Insect Resistance and Genetics; Plant tissue culture and regeneration; Phytase and its Applications
TL;DR:
A mutant Bowman-Birk gene was created that encoded an inactive high-sulfur product that was used to transform soybean line Asgrow 3237, and there was a marked decrease in the ability of extracts prepared from these seeds to inhibit trypsin and chymotrypsin despite the presence of Kunitztrypsin inhibitor.
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