@article{richard_holt_grozinger_2012, title={Effects of immunostimulation on social behavior, chemical communication and genome-wide gene expression in honey bee workers (Apis mellifera)}, volume={13}, journal={BMC Genomics}, author={Richard, F. J. and Holt, H. L. and Grozinger, C. M.}, year={2012} }
 @article{richard_schal_tarpy_grozinger_2011, title={Effects of Instrumental Insemination and Insemination Quantity on Dufour’s Gland Chemical Profiles and Vitellogenin Expression in Honey Bee Queens (Apis mellifera)}, volume={37}, ISSN={0098-0331 1573-1561}, url={http://dx.doi.org/10.1007/s10886-011-9999-z}, DOI={10.1007/s10886-011-9999-z}, abstractNote={Honey bee queens (Apis mellifera) mate in their early adult lives with a variable number of males (drones). Mating stimulates dramatic changes in queen behavior, physiology, gene expression, and pheromone production. Here, we used virgin, single drone- (SDI), and multi-drone- (MDI) inseminated queens to study the effects of instrumental insemination and insemination quantity on the pheromone profiles of the Dufour's gland, and the expression of the egg-yolk protein, vitellogenin, in the fat body. Age, environmental conditions, and genetic background of the queens were standardized to specifically characterize the effects of these treatments. Our data demonstrate that insemination and insemination quantity significantly affect the chemical profiles of the Dufour's gland secretion. Moreover, workers were more attracted to Dufour's gland extract from inseminated queens compared to virgins, and to the extract of MDI queens compared to extract of SDI queens. However, while there were differences in the amounts of some esters between MDI queens and the other groups, it appears that the differences in behavioral responses were elicited by subtle changes in the overall chemical profiles rather than dramatic changes in specific individual chemicals. We also found a decrease in vitellogenin gene expression in the fat body of the MDI queens, which is negatively correlated with the quantities of Dufour's gland content. The possible explanations of this reduction are discussed.}, number={9}, journal={Journal of Chemical Ecology}, publisher={Springer Science and Business Media LLC}, author={Richard, Freddie-Jeanne and Schal, Coby and Tarpy, David R. and Grozinger, Christina M.}, year={2011}, month={Jul}, pages={1027–1036} }
 @article{fan_richard_rouf_grozinger_2010, title={Effects of queen mandibular pheromone on nestmate recognition in worker honeybees, Apis mellifera}, volume={79}, number={3}, journal={Animal Behaviour}, author={Fan, Y. L. and Richard, F. J. and Rouf, N. and Grozinger, C. M.}, year={2010}, pages={649–656} }
 @article{kocher_richard_tarpy_grozinger_2009, title={Queen reproductive state modulates pheromone production and queen-worker interactions in honeybees}, volume={20}, ISSN={["1465-7279"]}, DOI={10.1093/beheco/arp090}, abstractNote={The mandibular glands of queen honeybees produce a pheromone that modulates many aspects of worker honeybee physiology and behavior and is critical for colony social organization. The exact chemical blend produced by the queen differs between virgin and mated, laying queens. Here, we investigate the role of mating and reproductive state on queen pheromone production and worker responses. Virgin queens, naturally mated queens, and queens instrumentally inseminated with either semen or saline were collected 2 days after mating or insemination. Naturally mated queens had the most activated ovaries and the most distinct chemical profile in their mandibular glands. Instrumentally inseminated queens were intermediate between virgins and naturally mated queens for both ovary activation and chemical profiles. There were no significant differences between semen- and saline-inseminated queens. Workers were preferentially attracted to the mandibular gland extracts from queens with significantly more activated ovaries. These studies suggest that the queen pheromone blend is modulated by the reproductive status of the queens, and workers can detect these subtle differences and are more responsive to queens with higher reproductive potential. Furthermore, it appears as if insemination substance does not strongly affect physiological characteristics of honeybee queens 2 days after insemination, suggesting that the insemination process or volume is responsible for stimulating these early postmating changes in honeybee queens.}, number={5}, journal={BEHAVIORAL ECOLOGY}, author={Kocher, Sarah D. and Richard, Freddie-Jeanne and Tarpy, David R. and Grozinger, Christina M.}, year={2009}, pages={1007–1014} }
 @article{kocher_richard_tarpy_grozinger_2008, title={Genomic analysis of post-mating changes in the honey bee queen (Apis mellifera)}, volume={9}, ISSN={["1471-2164"]}, DOI={10.1186/1471-2164-9-232}, abstractNote={Abstract}, journal={BMC GENOMICS}, author={Kocher, Sarah D. and Richard, Freddie-Jeanne and Tarpy, David R. and Grozinger, Christina M.}, year={2008}, month={May} }
 @article{richard_aubert_grozinger_2008, title={Modulation of social interactions by immune stimulation in honey bee, Apis mellifera, workers}, volume={6}, journal={BMC Biology}, author={Richard, F. J. and Aubert, A. and Grozinger, C. M.}, year={2008} }
 @article{aubert_richard_2008, title={Social management of LPS-induced inflammation in Formica polyctena ants}, volume={22}, ISSN={["1090-2139"]}, DOI={10.1016/j.bbi.2008.01.010}, abstractNote={Invertebrates, and especially insects, constitute valuable and convenient models for the study of the evolutionary roots of immune-related behaviors. With stable conditions in the nest, high population densities, and frequent interactions, social insects such as ants provide an excellent system for examining the spread of pathogens. The evolutionary success of these species raises questions about the behavioral responses of social insects to an infected nestmate. In this experiment, we tested the behavioral changes of the red wood ant Formica polyctena toward an immune-stimulated nestmate. We used bacterial endotoxin (lipopolysaccharides, LPS) to active the innate immune system of individual worker ants without biasing our observation with possible cues or host-manipulation from a living pathogen. We show that LPS-induced immune activation in ants triggers behavioral changes in nestmates. Contrary to what would be expected, we did not find removal strategies (e.g. agonistic behaviors) or avoidance of the pathogenic source, but rather a balance between a limitation of pathogen dissemination (i.e. decreased trophallaxis and locomotion of the LPS-treated ant), and what could constitute the behavioral basis for a “social vaccination” (i.e. increased grooming). This supports the importance of social interactions in resistance to disease in social insects, and perhaps social animals in general.}, number={6}, journal={BRAIN BEHAVIOR AND IMMUNITY}, author={Aubert, A. and Richard, F. -J.}, year={2008}, month={Aug}, pages={833–837} }
 @article{richard_poulsen_drijfhout_jones_boomsma_2007, title={Specificity in chemical profiles of workers, brood and mutualistic fungi in Atta, Acromyrmex, and Sericomyrmex fungus-growing ants}, volume={33}, ISSN={["1573-1561"]}, DOI={10.1007/s10886-007-9385-z}, abstractNote={Neotropical attine ants live in obligatory symbiosis with a fungus that they grow for food on a substrate of primarily plant material harvested by workers. Nestmate recognition is likely based on chemical cues as in most other social insects, but recent studies have indicated that both the ants and their mutualistic fungi may contribute to the recognition templates. To investigate the within-colony variation in chemical profiles, we extracted and identified compounds from the cuticle of workers, the postpharyngeal gland of workers, ant pupae and larvae, and the fungal symbiont of three species of higher attine ants: Atta colombica, Acromyrmex echinatior, and Sericomyrmex amabilis. The relative proportions of identified compounds were compared and represented 11 classes: n-alkanes, alkenes, branched methylalkanes, branched dimethylalkanes, trimethylalkanes, branched alkenes, aldehydes, alcohols, acetates, acids, and esters. The chemical profiles in all three species are likely to be sufficiently different to allow discrimination at the species and colony level and sufficiently similar within colonies to generate a relatively constant colony-specific chemical gestalt. The relative likelihood of individual compounds being derived from the ants, the ant brood, or the fungal symbiont are discussed. We hypothesize that hydrocarbons are particularly important as recognition cues because they appear to simultaneously allow the assessment of developmental stages and the identification of symbiont, colony, and species.}, number={12}, journal={JOURNAL OF CHEMICAL ECOLOGY}, author={Richard, Freddie-Jeanne and Poulsen, Michael and Drijfhout, Falko and Jones, Graeme and Boomsma, Jacobus J.}, year={2007}, month={Dec}, pages={2281–2292} }
 @article{richard_poulsen_hefetz_errard_nash_boomsma_2007, title={The origin of the chemical profiles of fungal symbionts and their significance for nestmate recognition in Acromyrmex leaf-cutting ants}, volume={61}, number={11}, journal={Behavioral Ecology and Sociobiology}, author={Richard, F. J. and Poulsen, M. and Hefetz, A. and Errard, C. and Nash, D. R. and Boomsma, J. J.}, year={2007}, pages={1637–1649} }