Timothy M. Orcutt

author keywords: Zebrafish; Mouse; rag1; Innate immunity; SCID

MeSH headings : Adaptation, Physiological / genetics; Adaptation, Physiological / immunology; Animals; Animals, Genetically Modified; Blood Coagulation Factors / genetics; Blood Coagulation Factors / metabolism; Complement C4 / genetics; Complement C4 / metabolism; Complement System Proteins / genetics; Complement System Proteins / metabolism; Female; Gene Expression Profiling; Genes, RAG-1 / physiology; Immunity / genetics; Immunity / physiology; Intestinal Mucosa / metabolism; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; Oligonucleotide Array Sequence Analysis; Transcription, Genetic; Up-Regulation; Zebrafish

TL;DR: It is hypothesized that the rag1(-/-) zebrafish may possess an "enhanced" innate immune response to compensate for the lack of an adaptive immune system. (via Semantic Scholar)

MeSH headings : Amino Acid Sequence; Animals; Conserved Sequence; Evolution, Molecular; Fish Proteins / genetics; Fish Proteins / immunology; Genetic Variation; Immunoglobulin Variable Region / genetics; Models, Genetic; Molecular Sequence Data; Multigene Family; Oryzias / genetics; Phylogeny; Protein Structure, Tertiary / genetics; Receptors, Immunologic / genetics; Species Specificity; Synteny; Zebrafish / genetics; Zebrafish / immunology

TL;DR: Comprehensive phylogenetic analyses between the complete datasets of NITRs from medaka and zebrafish indicate multiple species-specific expansions of different families of NITS, consistent with recent birth-and-death events. (via Semantic Scholar)

author keywords: DAP10; DAP12; FcR gamma; CD3 zeta

MeSH headings : Adaptor Proteins, Signal Transducing / chemistry; Adaptor Proteins, Signal Transducing / genetics; Adaptor Proteins, Signal Transducing / metabolism; Amino Acid Sequence; Animals; CD3 Complex / genetics; CD3 Complex / metabolism; Cell Lineage; Cell Membrane / immunology; Cell Membrane / metabolism; Cloning, Molecular; DNA, Complementary; Embryo, Nonmammalian; Gene Expression Regulation, Developmental; Genome; Killer Cells, Natural / immunology; Molecular Sequence Data; Phylogeny; Protein Structure, Tertiary; Receptors, IgG / immunology; Receptors, Immunologic / chemistry; Receptors, Immunologic / genetics; Receptors, Immunologic / metabolism; Receptors, KIR; Sequence Homology, Amino Acid; Zebrafish / embryology; Zebrafish / genetics; Zebrafish Proteins

TL;DR: In an effort to understand developmental aspects of immune receptor function, the partially annotated zebrafish genome is accessed to identify six different adaptor molecules that are homologous to those effecting immune function in mammals. (via Semantic Scholar)

author keywords: natural cytotoxicity; DAP10; ERK; AKT; DAP12; NITR

MeSH headings : Adaptor Proteins, Signal Transducing / genetics; Adaptor Proteins, Signal Transducing / immunology; Adaptor Proteins, Signal Transducing / metabolism; Amino Acid Sequence; Animals; Cell Line; Humans; Molecular Sequence Data; Phosphorylation; Receptors, KIR / genetics; Receptors, KIR / immunology; Receptors, KIR / metabolism; Recombinant Proteins / immunology; Recombinant Proteins / metabolism; Sequence Alignment; Signal Transduction / immunology; Zebrafish; Zebrafish Proteins / genetics; Zebrafish Proteins / immunology; Zebrafish Proteins / metabolism

TL;DR: Cross-linking the Nitr9L–Dap12 complex results in activation of the phosphytidylinositol 3-kinase→AKT→extracellular signal-regulated kinase pathway suggesting that the DAP12-based activating pathway is conserved between bony fish and mammals. (via Semantic Scholar)