@article{bodle_hanson_loboa_2011, title={Adipose-Derived Stem Cells in Functional Bone Tissue Engineering: Lessons from Bone Mechanobiology}, volume={17}, ISSN={["1937-3376"]}, DOI={10.1089/ten.teb.2010.0738}, abstractNote={This review aims to highlight the current and significant work in the use of adipose-derived stem cells (ASC) in functional bone tissue engineering framed through the bone mechanobiology perspective. Over a century of work on the principles of bone mechanosensitivity is now being applied to our understanding of bone development. We are just beginning to harness that potential using stem cells in bone tissue engineering. ASC are the primary focus of this review due to their abundance and relative ease of accessibility for autologous procedures. This article outlines the current knowledge base in bone mechanobiology to investigate how the knowledge from this area has been applied to the various stem cell-based approaches to engineering bone tissue constructs. Specific emphasis is placed on the use of human ASC for this application.}, number={3}, journal={TISSUE ENGINEERING PART B-REVIEWS}, author={Bodle, Josephine C. and Hanson, Ariel D. and Loboa, Elizabeth G.}, year={2011}, month={Jun}, pages={195–211} }
 @inbook{mccullen_hanson_lucia_loboa_2009, title={Development and application of naturally renewable scaffold materials for bone tissue engineering}, DOI={10.1002/9781444307474.ch11}, booktitle={Nanotechnology of renewable materials}, author={McCullen, S. D. and Hanson, A. D. and Lucia, Lucian and Loboa, E. G.}, editor={L. A. Lucia and Rojas, O. J.Editors}, year={2009} }
 @article{hanson_marvel_bernacki_banes_aalst_loboa_2009, title={Osteogenic Effects of Rest Inserted and Continuous Cyclic Tensile Strain on hASC Lines with Disparate Osteodifferentiation Capabilities}, volume={37}, ISSN={["1573-9686"]}, DOI={10.1007/s10439-009-9648-7}, abstractNote={We investigated the effects of two types of cyclic tensile strain, continuous and rest inserted, on osteogenic differentiation of human adipose-derived adult stem cells (hASCs). The influence of these mechanical strains was tested on two hASC lines having different mineral deposition potential, with one cell line depositing approximately nine times as much calcium as the other hASC line after 14 days of culture in osteogenic medium on tissue culture plastic. Results showed that both continuous (10% strain, 1 Hz) and rest inserted cyclic tensile strain (10% strain, 1 Hz, 10 s rest after each cycle) regimens increased the amount and rate of calcium deposition for both high and low calcium depositing hASC lines as compared to unstrained controls. The response was similar for both types of tensile strain for a given cell line, however, cyclic tensile strain had a much stronger osteogenic effect on the high calcium depositing hASC line, suggesting that mechanical loading has a greater effect on cell lines that already have an innate ability to produce bone as compared to cell lines that do not. This is the first study to investigate the osteodifferentiation effects of cyclic tensile strain on hASCs and the first to show that both continuous (10%, 1 Hz) and rest inserted (10%, 1 Hz, 10 s rest) cyclic tensile strain accelerate hASC osteodifferentiation and increase calcium accretion.}, number={5}, journal={ANNALS OF BIOMEDICAL ENGINEERING}, author={Hanson, Ariel D. and Marvel, Skylar W. and Bernacki, Susan H. and Banes, Albert J. and Aalst, John and Loboa, Elizabeth G.}, year={2009}, month={May}, pages={955–965} }
 @article{hanson_wall_pourdeyhimi_loboa_2007, title={Effects of oxygen plasma treatment on adipose-derived human mesenchymal stem cell adherence to poly(L-lactic acid) scaffolds}, volume={18}, ISSN={["1568-5624"]}, DOI={10.1163/156856207782246812}, abstractNote={Plasma treatment of substrate surfaces can be utilized to improve adhesion of cells to tissue-engineered scaffolds. The purpose of this study was to enhance cell adhesion to non-woven poly(L-lactic acid) (PLLA) scaffolds using oxygen plasma treatment to increase surface hydroxyl groups and thereby enhance substrate hydrophilicity. It was hypothesized that oxygen plasma treatment would increase the number of adipose-derived human mesenchymal stem cells (hMSCs) that adhered to melt-blown, non-woven PLLA scaffolds without affecting cell viability. The number of cells that adhered to the oxygen plasma-treated (10 min at 100 W) or untreated PLLA scaffolds was assessed at 2, 4, 8, 12, 24 and 48 h post-seeding via DNA analysis. Cell viability and morphology were also assessed at 2, 4, 8, 12 and 24 h post-seeding via a live/dead assay and hematoxylin staining, respectively. Oxygen plasma treatment decreased the contact angle of water from 75.6° to 58.2°, indicating an increase in the surface hydrophilicity of PLLA. The results of the DNA analysis indicated that there was an increased number of hMSCs on oxygen plasma treated scaffolds for two of the three donors. In addition, oxygen plasma treatment promoted a more even distribution of hMSCs throughout the scaffold and enhanced cell spreading at earlier time points without altering cell viability. This early induction of cell spreading and the uniform distribution of cells, in turn, may increase future proliferation and differentiation of hMSCs under conditions that simulate the microenvironment in vivo.}, number={11}, journal={JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION}, author={Hanson, Ariel D. and Wall, Michelle E. and Pourdeyhimi, Behnam and Loboa, Elizabeth G.}, year={2007}, month={Nov}, pages={1387–1400} }