Miguel Angel Flores-Vergara

Works (6)

Updated: July 5th, 2023 15:54

2017 journal article

A VIGS screen identifies immunity in the Arabidopsis Pla-1 accession to viruses in two different genera of the Geminiviridae

PLANT JOURNAL, 92(5), 796–807.

By: M. Reyes n, M. Flores-Vergara n, O. Guerra-Peraza  n, C. Rajabu *, J. Desai n, Y. Hiromoto-Ruiz n, J. Ndunguru *, L. Hanley-Bowdoin n  ...

author keywords: Arabidopsis; BCTV; CaLCuV; geminivirus; gip-1; immunity; Pla-1; VIGS
MeSH headings : Arabidopsis / virology; Geminiviridae / immunology; Gene Silencing; Plant Diseases / immunology; Plant Diseases / virology; Plant Immunity; Quantitative Trait Loci / genetics
TL;DR: The recessive nature of resistance to CaLCuV and the lack of obvious candidate genes near the gip‐1 locus suggest that a novel resistance gene(s) confers immunity. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2016 journal article

SEUSS Integrates Gibberellin Signaling with Transcriptional Inputs from the SHR-SCR-SCL3 Module to Regulate Middle Cortex Formation in the Arabidopsis Root

PLANT PHYSIOLOGY, 170(3), 1675–1683.

By: X. Gong *, M. Flores-Vergara n, J. Hong *, H. Chu *, J. Lim *, R. Franks n , Z. Liu *, J. Xu *, P. physiology

Contributors: X. Gong *, M. Flores-Vergara n, J. Hong *, H. Chu *, J. Lim *, R. Franks n , Z. Liu *, J. Xu *

MeSH headings : Arabidopsis / cytology; Arabidopsis / genetics; Arabidopsis / metabolism; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Co-Repressor Proteins / genetics; Co-Repressor Proteins / metabolism; Epistasis, Genetic; Gene Expression Regulation, Plant; Gibberellins / metabolism; Microscopy, Confocal; Models, Genetic; Mutation; Plant Roots / cytology; Plant Roots / genetics; Plant Roots / metabolism; Plants, Genetically Modified; Protein Binding; Reverse Transcriptase Polymerase Chain Reaction; Signal Transduction; Transcription Factors / genetics; Transcription Factors / metabolism; Two-Hybrid System Techniques
TL;DR: This study identifies SEU as a new critical player that integrates GA signaling with transcriptional inputs from the SHR-SCR-SCL3 module to regulate MC formation in the Arabidopsis root. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2016 journal article

Transcriptomic Signature of the <i>SHATTERPROOF2</i> Expression Domain Reveals the Meristematic Nature of Arabidopsis Gynoecial Medial Domain

Plant Physiology, 171(1), 42–61.

By: G. Villarino n, Q. Hu n, S. Manrique *, M. Flores-Vergara n, B. Sehra n, L. Robles n, J. Brumos  n, A. Stepanova n  ...

Contributors: G. Villarino n, Q. Hu n, S. Manrique *, M. Flores-Vergara n, B. Sehra n, L. Robles n, J. Brumos  n, A. Stepanova n  ...

MeSH headings : Arabidopsis / anatomy & histology; Arabidopsis / genetics; Arabidopsis Proteins / genetics; Arabidopsis Proteins / isolation & purification; Base Sequence; Chloral Hydrate; DNA, Antisense; Flowers / genetics; Gene Expression Regulation, Plant; Genome, Plant; In Situ Hybridization; Indoleacetic Acids / pharmacology; MADS Domain Proteins / genetics; MADS Domain Proteins / isolation & purification; Meristem / genetics; Meristem / growth & development; Meristem / metabolism; Microscopy, Confocal; Ovule / cytology; Ovule / growth & development; Ovule / metabolism; Protein Isoforms; Protoplasts; RNA, Plant / chemistry; RNA, Plant / isolation & purification; Seeds / growth & development; Sequence Alignment; Transcription Factors; Transcriptional Activation; Transcriptome
TL;DR: A novel approach to analyze the transcriptional signature of the medial domain of the Arabidopsis gynoecium, highlighting the developmental stages that immediately proceed ovule initiation, the earliest stages of seed development and identifying additional genes including novel isoforms, cis-natural antisense transcripts, and a previously unrecognized member of the REPRODUCTIVE MERISTEM family of transcriptional regulators that are potential novel regulators of medial domain development. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: August 6, 2018

2015 article

Persistent Virus-Induced Gene Silencing in Asymptomatic Accessions of Arabidopsis

PLANT FUNCTIONAL GENOMICS: METHODS AND PROTOCOLS, 2ND EDITION, Vol. 1284, pp. 305–322.

By: M. Flores  n, M. Reyes n, D. Robertson n & S. Kjemtrup*

MeSH headings : Arabidopsis / genetics; Arabidopsis / growth & development; Arabidopsis / virology; Begomovirus / physiology; Cloning, Molecular / methods; Gene Silencing; Gene Targeting / methods; Genetic Vectors / genetics; Host-Pathogen Interactions / genetics
TL;DR: It is shown that genetic variation in the vast collection of Arabidopsis accessions can be leveraged to ameliorate viral symptomology that accompanies the VIGS procedure. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2014 journal article

In Vivo Mapping of Arabidopsis Scaffold/Matrix Attachment Regions Reveals Link to Nucleosome-Disfavoring Poly(dA:dT) Tracts

PLANT CELL, 26(1), 102–120.

By: P. Pascuzzi n, M. Flores-Vergara n, T. Lee  n, B. Sosinski n, M. Vaughn *, L. Hanley-Bowdoin n , W. Thompson n , G. Allen n

MeSH headings : Arabidopsis / genetics; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Arabidopsis Proteins / physiology; Chromatin / metabolism; Gene Expression Regulation, Plant; Matrix Attachment Regions; Nucleosomes / metabolism; Nucleotide Motifs; Poly dA-dT / metabolism; Transcription Factors / genetics; Transcription Factors / metabolism; Transcription Factors / physiology
TL;DR: This work uses tiling microarrays to map S/MARs on Arabidopsis chromosome 4, found to be spaced more closely than in the large plant and animal genomes studied to date and preferentially enriched in poly(dA:dT) tracts, sequences that resist nucleosome formation. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2006 journal article

A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide

NATURE PROTOCOLS, 1(5), 2320–2325.

By: G. Allen n, M. Flores-Vergara n, S. Krasnyanski, S. Kumar n & W. Thompson n 

MeSH headings : Cetrimonium; Cetrimonium Compounds; DNA, Plant / isolation & purification; Magnoliopsida / chemistry; Plant Leaves / chemistry; Time Factors
TL;DR: The modified CTAB procedure is faster, omits the selective precipitation and CsCl gradient steps, uses less expensive and toxic reagents, requires only inexpensive laboratory equipment and is more readily adapted to high-throughput DNA extraction. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

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