@article{edwards_young_deiters_2009, title={Light-Activated Cre Recombinase as a Tool for the Spatial and Temporal Control of Gene Function in Mammalian Cells}, volume={4}, ISSN={["1554-8937"]}, DOI={10.1021/cb900041s}, abstractNote={Cre recombinase catalyzes DNA exchange between two conserved lox recognition sites. The enzyme has extensive biological application, from basic cloning to engineering knock-out and knock-in organisms. Widespread use of Cre is due to its simplicity and effectiveness, but the enzyme and the recombination event remain difficult to control with high precision. To obtain such control we report the installation of a light-responsive o-nitrobenzyl caging group directly in the catalytic site of Cre, inhibiting its activity. Prior to irradiation, caged Cre is completely inactive, as demonstrated both in vitro and in mammalian cell culture. Exposure to non-damaging UVA light removes the caging group and restores recombinase activity. Tight spatio-temporal control over DNA recombination is thereby achieved.}, number={6}, journal={ACS CHEMICAL BIOLOGY}, author={Edwards, Wesleigh F. and Young, Douglas D. and Deiters, Alexander}, year={2009}, month={Jun}, pages={441–445} }
 @article{edwards_young_deiters_2009, title={The effect of microwave irradiation on DNA hybridization}, volume={7}, ISSN={["1477-0539"]}, DOI={10.1039/b903609a}, abstractNote={The effect of microwave irradiation on DNA/DNA hybridization has been studied under controlled power and temperature conditions. It was discovered that microwave irradiation led to the melting of double-stranded deoxyoligonucleotides well below their thermal melting temperature and independent of the length of the deoxyoligonucleotides. These observations indicate a specific interaction of microwaves with DNA, and have important implications in the chemical or enzymatic processing of DNA under microwave heating.}, number={12}, journal={ORGANIC & BIOMOLECULAR CHEMISTRY}, author={Edwards, Wesleigh F. and Young, Douglas D. and Deiters, Alexander}, year={2009}, pages={2506–2508} }