Works (5)
Updated: July 24th, 2023 14:45
2010 journal article
Data-independent liquid chromatography/mass spectrometry (LC/MSE) detection and quantification of the secreted Apium graveolens pathogen defense protein mannitol dehydrogenase
Rapid Communications in Mass Spectrometry, 24(7), 1009–1016.
MeSH headings : Amino Acid Sequence; Apium / drug effects; Apium / enzymology; Cell Culture Techniques; Chromatography, Liquid / methods; Electrophoresis, Polyacrylamide Gel; Immunoblotting; Mannitol Dehydrogenases / chemistry; Mannitol Dehydrogenases / genetics; Mannitol Dehydrogenases / metabolism; Molecular Sequence Data; Proteome / analysis; Proteome / drug effects; Proteome / metabolism; Salicylic Acid / pharmacology; Tandem Mass Spectrometry / methods
TL;DR:
LC/MS(E) can be implemented as a discovery-driven MRM-based quantitative approach which can be used to reveal potential post-translational modifications, thus providing a new method in the area of gel-free and label-free proteomic analysis.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: NC State University Libraries
Added: August 6, 2018
2009 journal article
Absolute Protein Quantification by LC/MSE for Global Analysis of Salicylic Acid-induced Plant Protein Secretion Responses
JOURNAL OF PROTEOME RESEARCH, 8(1), 82–93.
author keywords: Arabidopsis; secretome; liquid chromatography; mass spectrometry; MSE; data-independent acquisition; salicylic acid; pathogen; nonclassical protein secretion
MeSH headings : Arabidopsis / drug effects; Arabidopsis / metabolism; Chromatography, Liquid / methods; Computational Biology; Mass Spectrometry / methods; Plant Physiological Phenomena; Plant Proteins / metabolism; Proteins / metabolism; Proteome; Proteomics / methods; Salicylic Acid / metabolism; Salicylic Acid / pharmacology; Time Factors
TL;DR:
A mass spectrometry, data-independent acquisition approach known as LC/MS (E) was used to assess temporal changes in the cell wall proteome in response to different levels of an endogenous inducer of plant disease defense responses, salicylic acid (SA).
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
15. Life on Land
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2009 journal article
Salicylic acid stimulates secretion of the normally symplastic enzyme mannitol dehydrogenase: a possible defense against mannitol-secreting fungal pathogens
PLANTA, 230(6), 1093–1103.
author keywords: Protein secretion; Mannitol metabolism; Plant-pathogen interaction; Extracellular space; Apoplast
MeSH headings : Antifungal Agents / pharmacology; Brefeldin A / pharmacology; Cell Wall / drug effects; Cell Wall / metabolism; Cells, Cultured; Cytoplasm / metabolism; Fungi / growth & development; Fungi / metabolism; Golgi Apparatus / drug effects; Golgi Apparatus / metabolism; Golgi Apparatus / ultrastructure; Immunity, Innate / genetics; Immunoblotting; Mannitol / metabolism; Mannitol Dehydrogenases / genetics; Mannitol Dehydrogenases / metabolism; Microscopy, Immunoelectron; Plant Diseases / genetics; Plant Diseases / microbiology; Plant Leaves / genetics; Plant Leaves / metabolism; Plant Leaves / ultrastructure; Plant Proteins / genetics; Plant Proteins / metabolism; Plants, Genetically Modified; Protein Transport / drug effects; Salicylic Acid / pharmacology; Tobacco / cytology; Tobacco / genetics; Tobacco / metabolism
TL;DR:
Immunoelectron microscopy, immunoblot, and biochemical data reported here show that the normally symplastic plant enzyme, mannitol dehydrogenase (MTD), is secreted into the apoplast after treatment with the endogenous inducer of plant defense responses salicylic acid (SA).
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2008 journal article
Polyols in plants and pathogens: An integration of transport and function
Current Topics in Plant Biology, 9, 101–114.
Source: NC State University Libraries
Added: August 6, 2018
2007 journal article
Leaf extracellular ascorbate in relation to O(3) tolerance of two soybean cultivars
ENVIRONMENTAL POLLUTION, 150(3), 355–362.
author keywords: antioxidant capacity; apoplast; ascorbic acid; dehydroascorbic acid; ozone tolerance; soybean
MeSH headings : Agriculture; Air Pollutants / toxicity; Antioxidants / analysis; Ascorbic Acid / analysis; Dehydroascorbic Acid / analysis; Ecology; Ozone / toxicity; Photosynthesis; Plant Leaves / chemistry; Plant Leaves / metabolism; Soybeans / metabolism; Species Specificity; Vitamins / analysis
TL;DR:
Investigation of soybean cultivars Essex and Forrest suggests that extracellular antioxidant metabolites in addition to ascorbate contribute to detoxification of O(3) in soybean leaves and possibly affect plant sensitivity to O( 3) injury.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
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