@article{bachlava_cardinal_2009, title={Correlation between Temperature and Oleic Acid Seed Content in Three Segregating Soybean Populations}, volume={49}, ISSN={["1435-0653"]}, DOI={10.2135/cropsci2008.11.0660}, abstractNote={The development of high‐oleate soybean [Glycine max (L.) Merr.] germplasm, currently in demand due to its nutritional value and oxidative stability, may be hampered by the instability of fatty acid composition across environments, which is largely attributed to temperature changes and its effects on fatty acid biosynthesis during the period of oil deposition. In the present study, we evaluated three soybean populations that segregated for oleate content and maturity at multiple environments in North Carolina. Oleate content was positively correlated with daily temperatures, averaged over the seed‐filling stage, for the experimental lines of the late‐maturing population but was negatively correlated for the lines of the early‐maturing populations. Oil content was positively correlated with the average daily temperature during seed filling in all three populations, regardless of their early‐ or late‐maturity profile. Negative correlations between oleate content and temperature during the period of oil deposition have not been previously reported. Moreover, in contrast to previous reports, maximum, minimum, and average daily temperatures were highly correlated during seed filling in all environments of this study. We conclude that cautious interpretation of these findings is necessary due to the correlation between temperature and photoperiod during the period of oil deposition.}, number={4}, journal={CROP SCIENCE}, author={Bachlava, Eleni and Cardinal, Andrea J.}, year={2009}, pages={1328–1335} }
 @article{bachlava_dewey_burton_cardinal_2009, title={Mapping and Comparison of Quantitative Trait Loci for Oleic Acid Seed Content in Two Segregating Soybean Populations}, volume={49}, ISSN={["1435-0653"]}, DOI={10.2135/cropsci2008.06.0324}, abstractNote={Soybean [Glycine max (L.) Merr.] produces 29.4% of the world's edible vegetable oil. An important determinant of the nutritional value and the oxidative stability of soybean oil is the oleic acid content. Elevation of the oleate content levels leads to the improvement of soybean oil quality. However, our knowledge of the genetic factors underlying oleate variation in soybean seeds remains incomplete, hampering the use of marker‐assisted selection in soybean breeding programs. We used a whole‐genome scan approach to identify oleate quantitative trait loci (QTLs) in a soybean population segregating for oleic acid content and a cognate segregating population to confirm oleate QTL. A novel oleate QTL with moderate effects was revealed on linkage group F in the proximity of the simple sequence repeat marker sat_309, which was confirmed in both populations across all environments tested. Furthermore, this study verified the existence of an oleate QTL with moderate effects in the proximity of FAD2‐1B isoform on linkage group I, which interacted epistatically with the oleate QTL on linkage group F. Oleate QTLs with moderate effects were also detected on linkage groups A2 and N only in one of the populations under study. Minor QTLs on linkage groups E, L, A1, and D2 confirmed previous mapping studies for oleate content in soybean.}, number={2}, journal={CROP SCIENCE}, author={Bachlava, Eleni and Dewey, Ralph E. and Burton, Joseph W. and Cardinal, Andrea J.}, year={2009}, pages={433–442} }
 @article{bachlava_dewey_burton_cardinal_2009, title={Mapping candidate genes for oleate biosynthesis and their association with unsaturated fatty acid seed content in soybean}, volume={23}, ISSN={["1572-9788"]}, DOI={10.1007/s11032-008-9246-7}, number={2}, journal={MOLECULAR BREEDING}, author={Bachlava, Eleni and Dewey, Ralph E. and Burton, Joseph W. and Cardinal, Andrea J.}, year={2009}, month={Feb}, pages={337–347} }
 @article{bachlava_burton_brownie_wang_auclair_cardinal_2008, title={Heritability of oleic acid content in soybean seed oil and its genetic correlation with fatty acid and agronomic traits}, volume={48}, ISSN={["1435-0653"]}, DOI={10.2135/cropsci2008.01.0049}, abstractNote={Oleate content is important for the nutritional value and oxidative stability of soybean [Glycine max (L.) Merr.] seed oil. Response to selection for higher oleate content depends on its heritability in breeding populations, and correlated responses of other fatty acid and agronomic traits to selection for oleate content depend on their genetic correlations with oleate. The objective of this study was to estimate the heritability of oleate content and to determine the correlation of oleate with other fatty acid and agronomic traits in three soybean populations segregating for major and minor oleate genes grown in multiple environments. One of the populations consisted of 721 lines, providing excellent precision for estimation of the genetic parameters. The results of this study indicated that heritability for oleate content was sufficiently high that early generation selection can be effective when practiced on unreplicated lines grown at a single environment. Significant negative correlations were observed between oleate and linoleate, oleate and linolenate, as well as oleate and palmitate in all three populations. Significant positive correlations were detected between palmitate and stearate in one population segregating for oleate genes and fapnc and fap1 alleles, which reduce palmitate content. In the same population we also observed a significant negative correlation between yield and oleate content, and positive correlations between yield and linoleate, and linolenate and palmitate contents.}, number={5}, journal={CROP SCIENCE}, author={Bachlava, Eleni and Burton, Joseph W. and Brownie, Cavell and Wang, Sanbao and Auclair, Jerome and Cardinal, Andrea J.}, year={2008}, pages={1764–1772} }
 @article{bachlava_dewey_auclair_wang_burton_cardinal_2008, title={Mapping genes encoding microsomal omega-6 desaturase enzymes and their cosegregation with QTL affecting oleate content in soybean}, volume={48}, ISSN={["1435-0653"]}, DOI={10.2135/cropsci2007.07.0381}, abstractNote={The microsomal ω‐6 desaturase enzymes, which catalyze the desaturation of oleic acid to linoleic acid during fatty acid biosynthesis, are encoded by the FAD2‐1 and FAD2‐2 genes in soybean [Glycine max (L.) Merr.]. Breeders aim to incorporate the high‐oleate trait into soybean germplasm in order to improve the nutritional value and oxidative stability of soybean oil. The objectives of this study were to map the isoforms of the FAD2‐1 and FAD2‐2 genes and investigate the association of these genetic loci with the oleate phenotype in three populations segregating for oleate content. The populations were grown in replicated multienvironment field trials. According to linkage analysis conducted for two of the populations, FAD2‐1A and FAD2‐1B mapped on Linkage Groups O and I, respectively, while the closely linked FAD2‐2A and FAD2‐2B isoforms mapped on Linkage Group L. Oleate quantitative trait loci with minor effects were detected in the proximity of FAD2‐1B and possibly FAD2‐2B on Linkage Groups I and L. Quantitative trait loci affecting maturity were also detected on chromosomal regions adjacent to the FAD2 genes. The genotyping assays developed for the FAD2‐1A, FAD2‐1B, and FAD2‐2B isoforms, as well as their linked simple sequence repeat markers, can be used in soybean breeding programs for the elevation of oleic acid seed content through marker‐assisted selection.}, number={2}, journal={CROP SCIENCE}, author={Bachlava, Eleni and Dewey, Ralph E. and Auclair, Jerome and Wang, Sanbao and Burton, Joseph W. and Cardinal, Andrea J.}, year={2008}, pages={640–650} }