Aaron Bradley Hawkins

Works (7)

Updated: August 9th, 2023 15:05

2016 journal article

Ancillary contributions of heterologous biotin protein ligase and carbonic anhydrase for CO2 incorporation into 3-hydroxypropionate by metabolically engineered Pyrococcus furiosus

Biotechnology and Bioengineering, 113(12), 2652–2660.

By: H. Lian, B. Zeldes, G. Lipscomb, A. Hawkins, Y. Han, A. Loder, D. Nishiyama, M. Adams, R. Kelly

Source: NC State University Libraries
Added: August 6, 2018

2016 journal article

Reaction kinetic analysis of the 3-hydroxypropionate/4-hydroxybutyrate CO2 fixation cycle in extremely thermoacidophilic archaea

METABOLIC ENGINEERING, 38, 446–463.

By: A. Loder n, Y. Han n, A. Hawkins n, H. Lian n, G. Lipscomb*, G. Schut *, M. Keller *, M. Adams *, R. Kelly n 

author keywords: CO2 fixation; 3-hydroxypropionate; 4-hydroxybutyrate; Metallosphaera sedula
MeSH headings : Archaea / metabolism; Carbon Dioxide / metabolism; Extremophiles / metabolism; Hydroxybutyrates / metabolism; Kinetics; Lactic Acid / analogs & derivatives; Lactic Acid / metabolism; Metabolic Clearance Rate; Metabolic Flux Analysis / methods; Metabolic Networks and Pathways / physiology; Models, Biological; Signal Transduction / physiology; Sulfolobaceae / metabolism
TL;DR: A reaction kinetics model was developed to examine the biological and biotechnological attributes of the 3HP/4HB cycle as it operates in Metallosphaera sedula and assess metabolic engineering strategies for incorporating CO2 into chemical intermediates and products of bioteschnological importance: acetyl-CoA, succinate, and 3-hydroxypropionate. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

Bioprocessing analysis of Pyrococcus furiosus strains engineered for CO2-based 3-hydroxypropionate production

BIOTECHNOLOGY AND BIOENGINEERING, 112(8), 1533–1543.

By: A. Hawkins n, H. Lian n, B. Zeldes n, A. Loder n, G. Lipscomb*, G. Schut *, M. Keller *, M. Adams *, R. Kelly n 

author keywords: 3-hydroxypropionate; CO2 fixation; Metallosphaera sedula; Pyrococcus furiosus
MeSH headings : Bioreactors / microbiology; Carbon Dioxide / metabolism; Gene Expression Profiling; Hot Temperature; Lactic Acid / analogs & derivatives; Lactic Acid / metabolism; Maltose / metabolism; Metabolic Engineering / methods; Metabolic Networks and Pathways / genetics; Pyrococcus furiosus / genetics; Pyrococcus furiosus / metabolism; Pyrococcus furiosus / radiation effects; Sulfolobaceae / genetics
TL;DR: The results here support the prospects of using thermally diverse sources of pathways and enzymes in metabolically engineered strains designed for product formation at sub‐optimal growth temperatures. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 journal article

Conversion of 4-Hydroxybutyrate to Acetyl Coenzyme A and Its Anapleurosis in the Metallosphaera sedula 3-Hydroxypropionate/4-Hydroxybutyrate Carbon Fixation Pathway

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 80(8), 2536–2545.

By: A. Hawkins n, M. Adams * & R. Kelly n 

MeSH headings : Acetyl Coenzyme A / metabolism; Carbon Cycle; Escherichia coli / genetics; Gene Expression Profiling; Metabolic Networks and Pathways / genetics; Recombinant Proteins / genetics; Recombinant Proteins / isolation & purification; Recombinant Proteins / metabolism; Sodium Oxybate / metabolism; Sulfolobaceae / metabolism; Transcriptome
TL;DR: Transcriptomic analysis revealed the importance of the 3HP/4HB pathway in supplying acetyl-CoA to anabolic pathways generating intermediates in M. sedula metabolism, and provided the framework for examining connections between CO2 fixation and central metabolism in the sedula. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 review

Biological conversion of carbon dioxide and hydrogen into liquid fuels and industrial chemicals

[Review of ]. CURRENT OPINION IN BIOTECHNOLOGY, 24(3), 376–384.

By: A. Hawkins n, P. McTernan *, H. Lian n, R. Kelly n  & M. Adams *

MeSH headings : Bioelectric Energy Sources; Biotechnology / methods; Carbon Cycle / genetics; Carbon Dioxide / metabolism; Chemical Industry; Hydrogen / metabolism; Metabolic Engineering / methods
TL;DR: This review examines the current landscape of electrofuel projects with a focus on hydrogen-utilizing organisms covering the biochemistry of hydrogenases and carbonic anhydrases, kinetic and energetic analyses of the known carbon fixation pathways, and the state of genetic systems for current and prospective electrofuel-producing microorganisms. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 journal article

Exploiting microbial hyperthermophilicity to produce an industrial chemical, using hydrogen and carbon dioxide

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 110(15), 5840–5845.

By: M. Keller *, G. Schut *, G. Lipscomb*, A. Menon*, I. Iwuchukwu*, T. Leuko*, M. Thorgersen *, W. Nixon* ...

author keywords: anaerobe; archaea; biotechnology; metabolic engineering; thermophile
MeSH headings : Carbohydrates / chemistry; Carbon Dioxide / chemistry; Gases; Genetic Engineering; Hydrogen / chemistry; Industrial Microbiology / methods; Lactic Acid / analogs & derivatives; Lactic Acid / biosynthesis; Lactic Acid / chemistry; Operon; Polymerase Chain Reaction; Pyrococcus furiosus / genetics; Pyrococcus furiosus / growth & development; Pyrococcus furiosus / metabolism; Temperature
TL;DR: A unique temperature-dependent approach is described that confers on a microorganism (the archaeon Pyrococcus furiosus, which grows optimally on carbohydrates at 100°C) the capacity to use carbon dioxide, a reaction that it does not accomplish naturally. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 journal article

Role of 4-Hydroxybutyrate-CoA Synthetase in the CO2 Fixation Cycle in Thermoacidophilic Archaea

JOURNAL OF BIOLOGICAL CHEMISTRY, 288(6), 4012–4022.

By: A. Hawkins n, Y. Han n, R. Bennett n, M. Adams * & R. Kelly n 

MeSH headings : Archaeal Proteins / chemistry; Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Carbon Dioxide / metabolism; Catalysis; Catalytic Domain; Coenzyme A-Transferases / chemistry; Coenzyme A-Transferases / genetics; Coenzyme A-Transferases / metabolism; Genome, Archaeal / physiology; Hydroxybutyrates / chemistry; Hydroxybutyrates / metabolism; Sulfolobaceae / enzymology; Sulfolobaceae / genetics
TL;DR: Transcriptomic analysis of cells grown under strict H2-CO2 autotrophy was consistent with the involvement of Msed_0406 and Msed-0394, and the identification of the 4-HB CoA synthetase now completes the set of enzymes comprising the 3HP/4HB cycle. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

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