@article{li_bruneau_qu_2010, title={Morphological mutants of St. Augustinegrass induced by gamma ray irradiation}, volume={129}, ISSN={["1439-0523"]}, DOI={10.1111/j.1439-0523.2009.01735.x}, abstractNote={With 3 figures and 3 tables Abstract St. Augustinegrass is a widely used turf and pasture grass in the southern US. ‘Raleigh’ is a cultivar known for superior cold tolerance than other St. Augustinegrass cultivars. However, its coarse-leaf texture and long internodes are undesirable when planted in home lawns. Mutagenesis by gamma ray irradiation was employed to treat node cuttings and calli for inducing semi-dwarf growth phenotype. Dosages of 48.5 and 72.6 Gy were determined as LD50 and LD20 for the cuttings, respectively. Regeneration ability of callus was greatly reduced when irradiated with higher dosages (over 100 Gy). Thirteen morphological mutants were identified among over 3000 node cuttings and 80 pieces of calli treated. Most mutants were semi-dwarf type with reduced internode length and leaf blade length. One mutant had much less and shorter stolons and displayed an upright and tufty growth pattern. The altered morphological traits were stable as shown by their growth performance in various locations and conditions.}, number={4}, journal={PLANT BREEDING}, author={Li, R. and Bruneau, A. H. and Qu, R.}, year={2010}, month={Aug}, pages={412–416} } @article{li_qu_bruneau_livingston_2010, title={Selection for freezing tolerance in St. Augustinegrass through somaclonal variation and germplasm evaluation}, volume={129}, ISSN={["1439-0523"]}, DOI={10.1111/j.1439-0523.2009.01743.x}, abstractNote={With 4 figures and 1 table Abstract St. Augustinegrass [Stenotaphrum secundatum (Walt.) Kuntze] is the least cold-hardy turfgrass species. Development of freezing-tolerant St. Augustinegrass cultivars would greatly benefit home owners in many southern states of the US. Towards this breeding goal, 7800 plants regenerated through tissue culture and 36 germplasm accessions were screened for improved freezing tolerance. Among the conditions tested, 1 week at 13°C followed by another week at 3°C, then freezing at −3 to −5°C for 3 h, was found to be suitable to distinguish genotypes in freezing tests. The experiments revealed that germplasm accession Elm4 was significantly more freezing-tolerant under a controlled environment than ‘Raleigh’, the current commercially available, most freezing-tolerant cultivar. In addition, out of 7800 regenerated plants from tissue culture, somaclonal variant SVC3 showed significantly more freezing-tolerant than its parent ‘Raleigh’.}, number={4}, journal={PLANT BREEDING}, author={Li, R. and Qu, R. and Bruneau, A. H. and Livingston, D. P.}, year={2010}, month={Aug}, pages={417–421} } @article{li_bruneau_qu_2010, title={Tissue culture-induced morphological somaclonal variation in St. Augustinegrass [Stenotaphrum secundatum (Walt.) Kuntze]}, volume={129}, ISSN={["1439-0523"]}, DOI={10.1111/j.1439-0523.2009.01647.x}, abstractNote={Somaclonal variation has been observed in many plant species and is an alternative way to create variants and expand the germplasm pool. A large scale tissue culture experiment was conducted with St. Augustinegrass, an important turfgrass species for the southern USA, to induce somaclonal variation to enlarge the germplasm pool for breeding efforts. Using an improved protocol, approximately 7900 St. Augustinegrass plants were regenerated from cv. Raleigh, and 119 morphological variants were identified. Among the variants, 115 had a semi-dwarf growth habit with shorter and narrower leaves, and shortened internodes and stolons. However, 100 of them showed little vigour, which either grew very slowly or did not survive. The remaining 15 showed reasonable growth vigour and were further investigated in the field. Among them, 13 were semi-dwarf and 2 had longer leaves. In addition, 2 other variants, with variegated (yellow striping) leaves, or significantly thicker stems were also observed and characterized. The altered traits in the variant lines were stable during vegetative propagation and when grown in different environments.}, number={1}, journal={PLANT BREEDING}, author={Li, R. and Bruneau, A. H. and Qu, R.}, year={2010}, month={Feb}, pages={96–99} } @article{li_bruneau_qu_2006, title={Improved plant regeneration and in vitro somatic embryogenesis of St Augustinegrass [Stenotaphrum secundatum (Walt.) Kuntze]}, volume={125}, ISSN={["1439-0523"]}, DOI={10.1111/j.1439-0523.2006.01193.x}, abstractNote={AbstractSt Augustinegrass [Stenotaphrum secundatum (Walt.) Kuntze] is an important warm season turf and pasture grass. In vitro tissue culture of St Augustinegrass could serve as an important mean for its improvement through genetic transformation as well as induced somaclonal variation. To optimize tissue culture conditions for plant regeneration of St Augustinegrass, tissue culture responses of 11 explant tissues and four callus induction/subculture media have been examined. Embryogenic calli with regeneration potential were observed on cultures of early immature embryo [3 days after pollination (DAP)], immature embryo (7–14 DAP), and shoot base of young seedlings. The addition of benzyladenine (BA) in the callus induction/subculture medium enhances callus regeneration ability and does not harm callus induction for immature embryos. The best response came from 7 to 14 DAP immature embryo on MS medium containing 1 mg/l 2,4‐dichlorophenoxyacetic acid and 0.5 mg/l BA. The callus induction and regeneration rates were 97.7% and 47.6% respectively. However, BA supplement reduced callus formation and failed to enhance regeneration for young leaf bases. Scanning electron microscopy revealed that plant regeneration of St Augustinegrass is via somatic embryogenesis.}, number={1}, journal={PLANT BREEDING}, author={Li, R and Bruneau, AH and Qu, R}, year={2006}, month={Feb}, pages={52–56} } @article{reynolds_butler_wetzel_bruneau_tredway_2005, title={Performance of Kentucky bluegrass-tall fescue mixtures in the Southeastern United States}, volume={10}, journal={International Turfgrass Society Research Journal}, author={Reynolds, W. C. and Butler, E. L. and Wetzel, H. C. and Bruneau, A. H. and Tredway, L. P.}, year={2005}, pages={525} } @article{chen_yang_sivamani_bruneau_wang_qu_2005, title={Selective elimination of perennial ryegrass by activation of a pro-herbicide through engineering E-coli argE gene}, volume={15}, ISSN={["1572-9788"]}, DOI={10.1007/s11032-004-7243-z}, number={4}, journal={MOLECULAR BREEDING}, author={Chen, X and Yang, WQ and Sivamani, E and Bruneau, AH and Wang, BH and Qu, RD}, year={2005}, month={May}, pages={339–347} } @article{bruneau_peacock_cooper_erickson_2004, title={Cynodon spp. management programs for the upper transition zone in the Southeastern United States}, ISBN={["90-6605-306-2"]}, ISSN={["0567-7572"]}, DOI={10.17660/actahortic.2004.661.75}, number={661}, journal={PROCEEDINGS OF THE 1ST INTERNATIONAL CONFERENCE ON TURFGRASS MANAGEMENT AND SCIENCE SPORTS FIELDS}, author={Bruneau, AH and Peacock, CH and Cooper, RJ and Erickson, EJ}, year={2004}, pages={551–557} } @article{peacock_lee_reynolds_gregg_cooper_bruneau_2004, title={Effects of salinity on six bermudagrass turf cultivars}, ISBN={["90-6605-306-2"]}, ISSN={["0567-7572"]}, DOI={10.17660/actahortic.2004.661.24}, number={661}, journal={PROCEEDINGS OF THE 1ST INTERNATIONAL CONFERENCE ON TURFGRASS MANAGEMENT AND SCIENCE SPORTS FIELDS}, author={Peacock, CH and Lee, DJ and Reynolds, WC and Gregg, JP and Cooper, RJ and Bruneau, AH}, year={2004}, pages={193–197} } @article{bradley_bruneau_qu_2001, title={Effects of cultivar, explant treatment, and medium supplements on callus induction and plantlet regeneration in perennial ryegrass}, volume={9}, number={2001}, journal={International Turfgrass Society Research Journal}, author={Bradley, D. E. and Bruneau, A. H. and Qu, R.}, year={2001}, pages={152–156} } @article{bruneau_bigelow_cooper_bowman_2001, title={Performance of creeping bentgrass cultivars maintained at two mowing heights and under two fungicide regimes in North Carolina}, volume={9}, journal={International Turfgrass Society Research Journal}, author={Bruneau, A. H. and Bigelow, C. A. and Cooper, R. J. and Bowman, D. C.}, year={2001}, pages={835} } @article{bradley_bruneau_qu_2001, title={Scanning electron microscopic study on in vitro somatic embryogenesis in perennial ryegrass and tall fescue}, volume={9}, number={2001}, journal={International Turfgrass Society Research Journal}, author={Bradley, D. E. and Bruneau, A. H. and Qu, R.}, year={2001}, pages={146–151} } @article{bruneau_newell_crossley_2000, title={Comparative performance of bentgrass species and cultivars in close mown turf}, volume={76}, ISBN={1367-8361}, journal={Journal of Turfgrass Science}, author={Bruneau, A. H. and Newell, A. J. and Crossley, F. M. E.}, year={2000}, pages={63–69} } @article{arumuganathan_tallury_fraser_bruneau_qu_1999, title={Nuclear DNA content of thirteen turfgrass species by flow cytometry}, volume={39}, number={5}, journal={Crop Science}, author={Arumuganathan, K. and Tallury, S. P. and Fraser, M. L. and Bruneau, A. H. and Qu, R.}, year={1999}, pages={1518–1521} }