Works (98)
Updated: December 6th, 2023 05:03
2023 article
Dissection of MKK6 and p38 Signaling Using Light-Activated Protein Kinases
Rahman, S. M. T., Zhou, W., Deiters, A., & Haugh, J. M. (2023, November 7). CHEMBIOCHEM, Vol. 11.
author keywords: apoptosis; autophagy; cell death; growth factors; mitogen-activated protein kinases; optogenetics; signal transduction
topics (OpenAlex): Autophagy in Disease and Therapy; Melanoma and MAPK Pathways; Sirtuins and Resveratrol in Medicine
TL;DR:
Although decaging active p38α does not reduce basal ERK activity in cells, it can block growth factor‐stimulated ERK signaling in serum‐starved cells, which clarifies the roles of MKK6 and p38α in dynamic signaling programs, which act in concert to actuate apoptotic death while suppressing cell survival mechanisms.
(via Semantic Scholar)
open access via onlinelibrary.wiley.com (publisher PDF)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: December 4, 2023
2023 article
Isoform-specific optical activation of kinase function reveals p38-ERK signaling crosstalk
Zhou, W., Ryan, A., Janosko, C. P., Shoger, K. E., Haugh, J. M., Gottschalk, R. A., & Deiters, A. (2023, August 25). RSC CHEMICAL BIOLOGY, Vol. 8.
topics (OpenAlex):
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: October 2, 2023
2022 article
Transcriptional Inhibition of MicroRNA miR-122 by Small Molecules Reduces Hepatitis C Virus Replication in Liver Cells
Emanuelson, C., Ankenbruck, N., Kumbhare, R., Thomas, M., Connelly, C., Baktash, Y., … Deiters, A. (2022, November 30). JOURNAL OF MEDICINAL CHEMISTRY.
MeSH headings : Humans; Hepacivirus / genetics; MicroRNAs / metabolism; Hepatitis C / drug therapy; Liver Neoplasms / pathology; Virus Replication
topics (OpenAlex): MicroRNA in disease regulation; RNA modifications and cancer; RNA Research and Splicing
TL;DR:
Preliminary mechanism-of-action studies suggest a role in the promoter-specific transcriptional inhibition of miR-122 expression through direct binding to the liver-enriched transcription factor hepatocyte nuclear factor 4α, and the developed inhibitors significantly reduce HCV replication in human liver cells.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: December 19, 2022
2020 journal article
Optical control of MAP kinase kinase 6 (MKK6) reveals that it has divergent roles in pro-apoptotic and anti-proliferative signaling
JOURNAL OF BIOLOGICAL CHEMISTRY, 295(25), 8494–8504.
author keywords: optogenetics; signal transduction; cell signaling; mitogen-activated protein kinases; apoptosis; cell death; cell stress; mitogen-activated protein kinase (MAPK)
MeSH headings : Animals; Apoptosis / radiation effects; Cell Line; Cell Proliferation / radiation effects; Fibroblasts / cytology; Fibroblasts / metabolism; Humans; MAP Kinase Kinase 6 / genetics; MAP Kinase Kinase 6 / metabolism; Mice; Mitogen-Activated Protein Kinase 1 / metabolism; Mitogen-Activated Protein Kinase 3 / metabolism; Mutagenesis; Protein Isoforms / antagonists & inhibitors; Protein Isoforms / metabolism; Proto-Oncogene Proteins B-raf / genetics; Proto-Oncogene Proteins B-raf / metabolism; Signal Transduction / radiation effects; Ultraviolet Rays; p38 Mitogen-Activated Protein Kinases / antagonists & inhibitors; p38 Mitogen-Activated Protein Kinases / metabolism
topics (OpenAlex): Melanoma and MAPK Pathways; Retinal Development and Disorders; Redox biology and oxidative stress
TL;DR:
MKK6 is position MKK6 as a new pleiotropic signal transducer that promotes both pro-apoptotic and anti-proliferative signaling, and the utility of caged, light-activated kinases for dissecting stress-activated signaling networks is highlighted.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: July 27, 2020
2015 journal article
A concise synthesis of the Lycopodium alkaloid cermizine D
TETRAHEDRON LETTERS, 56(23), 3683–3685.
author keywords: Alkaloid synthesis; Lycopodium alkaloids; Nitrogen heterocycles; 1-Acylpyridinium salts; Asymmetric synthesis
topics (OpenAlex): Cholinesterase and Neurodegenerative Diseases; Chemical synthesis and alkaloids; Botanical Research and Chemistry
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2015 journal article
Conditional Control of Alternative Splicing through Light-Triggered Splice-Switching Oligonucleotides
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 137(10), 3656–3662.
MeSH headings : Alternative Splicing / radiation effects; Animals; HeLa Cells; Humans; Light; Oligonucleotides / chemistry; Oligonucleotides / genetics; Zebrafish
topics (OpenAlex): Photochromic and Fluorescence Chemistry; RNA Interference and Gene Delivery; DNA and Nucleic Acid Chemistry
TL;DR:
This work addressed site-specific installation of light-removable nucleobase-caging groups as well as photocleavable backbone linkers into synthetic SSOs that enables optochemical OFF → ON and ON → OFF switching of their activity and thus precise control of alternative splicing.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2015 journal article
Light-cleavable rapamycin dimer as an optical trigger for protein dimerization
CHEMICAL COMMUNICATIONS, 51(26), 5702–5705.
MeSH headings : Humans; Molecular Conformation; Optics and Photonics; Photolysis / radiation effects; Protein Multimerization / radiation effects; Sirolimus / chemistry; Sirolimus / radiation effects; Tacrolimus Binding Protein 1A / chemistry; Ultraviolet Rays
topics (OpenAlex): Photoreceptor and optogenetics research; Photochromic and Fluorescence Chemistry; Click Chemistry and Applications
TL;DR:
An optically activated rapamycin dimer is developed that does not induce FKBP12-FRB dimerization until exposed to light, and applied to control kinase, protease, and recombinase function.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2015 journal article
Optically triggered immune response through photocaged oligonucleotides
TETRAHEDRON LETTERS, 56(23), 3639–3642.
author keywords: Oligonucleotide synthesis; Light-activation; Photocaged nucleotides; Immune response; Toll-like receptors
topics (OpenAlex): Photochromic and Fluorescence Chemistry; Nanoplatforms for cancer theranostics; Photodynamic Therapy Research Studies
TL;DR:
Synthetic photocaged CpGs via site-specific incorporation of nitropiperonyloxymethyl (NPOM)-caged thymidine residues enable the optical control of TLR9 function and thereby provide light-activation of an immune response.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2014 journal article
Control of Protein Function through Optochemical Translocation
ACS SYNTHETIC BIOLOGY, 3(10), 731–736.
author keywords: protein control; optogenetics; nuclear import; photocontrolled transcription; photocontrolled TEV-cleavage
MeSH headings : Active Transport, Cell Nucleus / radiation effects; Amino Acid Sequence; Endopeptidases / genetics; Endopeptidases / metabolism; Endopeptidases / radiation effects; Forkhead Box Protein O3; Forkhead Transcription Factors / genetics; Forkhead Transcription Factors / metabolism; Forkhead Transcription Factors / radiation effects; HEK293 Cells; Humans; Light; Matrix Attachment Region Binding Proteins / genetics; Matrix Attachment Region Binding Proteins / metabolism; Matrix Attachment Region Binding Proteins / radiation effects; Molecular Sequence Data; Nuclear Localization Signals / genetics; Photochemical Processes; Protein Engineering / methods; Synthetic Biology
topics (OpenAlex): Photochromic and Fluorescence Chemistry; Light effects on plants; bioluminescence and chemiluminescence research
TL;DR:
Since the light-activated NLS is genetically encoded and optically triggered, it will prove useful to address a variety of problems requiring spatial and temporal control of protein function, for example, in stem-cell, developmental, and cancer biology.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2014 journal article
Genetic Encoding of Caged Cysteine and Caged Homocysteine in Bacterial and Mammalian Cells
CHEMBIOCHEM, 15(12), 1793–1799.
author keywords: amino acids; caged compounds; cysteine; gene technology; homocysteine; protein modifications
MeSH headings : Cells, Cultured; Cysteine / chemical synthesis; Cysteine / chemistry; Cysteine / genetics; Escherichia coli / cytology; Escherichia coli / genetics; Genetic Code / genetics; HEK293 Cells; Homocysteine / chemical synthesis; Homocysteine / chemistry; Homocysteine / genetics; Humans; Luciferases, Renilla / chemistry; Luciferases, Renilla / metabolism; Models, Molecular; Molecular Structure
topics (OpenAlex): Advanced biosensing and bioanalysis techniques; DNA and Nucleic Acid Chemistry; Click Chemistry and Applications
TL;DR:
The genetic incorporation of caged cysteine and caged homocysteine into proteins in bacterial and mammalian cells is reported to provide a new tool for the optochemical control of protein function in mammalian cells and expand the set of genetically encoded unnatural amino acids.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2014 journal article
Genetically encoded unstrained olefins for live cell labeling with tetrazine dyes
CHEMICAL COMMUNICATIONS, 50(86), 13085–13088.
MeSH headings : Alkenes / chemistry; Amino Acids / metabolism; Amino Acyl-tRNA Synthetases / genetics; Amino Acyl-tRNA Synthetases / metabolism; Bacterial Outer Membrane Proteins / chemistry; Bacterial Outer Membrane Proteins / metabolism; Cycloaddition Reaction; Escherichia coli / metabolism; Escherichia coli Proteins / chemistry; Escherichia coli Proteins / metabolism; Fluorescein / chemistry; Fluorescent Dyes / chemistry; Green Fluorescent Proteins / chemistry; Heterocyclic Compounds, 1-Ring / chemistry; Hydrolases / chemistry; Hydrolases / metabolism; Mutation; RNA, Transfer / metabolism
topics (OpenAlex): Click Chemistry and Applications; Chemical Synthesis and Analysis; Advanced biosensing and bioanalysis techniques
TL;DR:
A number of non-canonical amino acids (NCAAs) with unstrained olefins are genetically encoded using mutant pyrrolysyl-t RNA synthetase-tRNA(Pyl)(CUA) pairs, leading to selective labeling of proteins bearing these NCAAs in live cells.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2014 article
Genetically encoded unstrained olefins for live cell labeling with tetrazine dyes (vol 50, pg 13085, 2014)
Lee, Y.-J., Kurra, Y., Yang, Y., Torres-Kolbus, J., Deiters, A., & Liu, W. R. (2014, November 11). CHEMICAL COMMUNICATIONS, Vol. 50, pp. 13396–13396.
topics (OpenAlex): bioluminescence and chemiluminescence research; Click Chemistry and Applications
TL;DR:
Genetically encoded unstrained olefins for live cell labeling with tetrazine dyes is shown to be a viable alternative for high-performance liquid chromatography of Na6(CO3)(SO4)2, Na2SO4, and Na2CO3 for cell labeling.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2014 journal article
Intracellular Light-Activation of Riboswitch Activity
ChemBioChem, 15(9), 1346–1351.
author keywords: chemical biology; photocontrol; riboswitches; RNA; synthetic biology
MeSH headings : Cell Membrane Permeability; Gene Expression Regulation / drug effects; Gene Expression Regulation / radiation effects; Intracellular Space / drug effects; Intracellular Space / genetics; Intracellular Space / radiation effects; Ligands; Molecular Structure; Riboswitch / drug effects; Riboswitch / genetics; Riboswitch / radiation effects; Structure-Activity Relationship; Theophylline / chemistry; Theophylline / pharmacology; Ultraviolet Rays
topics (OpenAlex): Photochromic and Fluorescence Chemistry; Antimicrobial Peptides and Activities; RNA and protein synthesis mechanisms
TL;DR:
By combining a riboswitch with a cell‐permeable photocaged small‐molecule ligand, an optochemical gene control element was constructed that enabled spatial and temporal control of gene expression in bacterial cells.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018
2014 journal article
MicroRNA Targeting of CoREST Controls Polarization of Migrating Cortical Neurons
CELL REPORTS, 7(4), 1168–1183.
MeSH headings : Animals; Cell Movement / physiology; Cell Polarity / physiology; Cells, Cultured; Cerebral Cortex / cytology; Co-Repressor Proteins; Mice; Mice, Transgenic; MicroRNAs / metabolism; Nerve Tissue Proteins / metabolism; Neurons / cytology; Neurons / metabolism; Repressor Proteins / metabolism
topics (OpenAlex): MicroRNA in disease regulation; Circular RNAs in diseases; Neurogenesis and neuroplasticity mechanisms
TL;DR:
This work describes how microRNAs (miRNAs) control neuronal polarization during radial migration and emphasizes key roles of some miRNAs that control radial migration during cerebral corticogenesis.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2014 review
Optochemical Control of Deoxyoligonucleotide Function via a Nucleobase-Caging Approach
[Review of ]. ACCOUNTS OF CHEMICAL RESEARCH, 47(1), 45–55.
MeSH headings : Base Sequence; DNA, Catalytic / genetics; DNA, Catalytic / metabolism; Humans; Light; Oligonucleotides / chemistry; Oligonucleotides / metabolism; Photochemical Processes; Proteins / metabolism; RNA / chemistry; RNA / metabolism
topics (OpenAlex): Photochromic and Fluorescence Chemistry; Advanced biosensing and bioanalysis techniques; DNA and Nucleic Acid Chemistry
TL;DR:
The application of caged nucleobases to the photoregulation of DNA function is focused on and light-activated DNA logic operations are developed, an advance that may lay the foundation for the optochemical control of complex DNA calculations.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2014 journal article
Site-Specific Promoter Caging Enables Optochemical Gene Activation in Cells and Animals
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 136(19), 7152–7158.
MeSH headings : Animals; Base Sequence; DNA, Circular / chemistry; DNA, Circular / genetics; Green Fluorescent Proteins / genetics; HEK293 Cells; Humans; Molecular Sequence Data; Photolysis; Plasmids / chemistry; Plasmids / genetics; Promoter Regions, Genetic; Protein Serine-Threonine Kinases / genetics; Transcriptional Activation; Tumor Suppressor Proteins; Ultraviolet Rays; Zebrafish
topics (OpenAlex): Photoreceptor and optogenetics research; Photochromic and Fluorescence Chemistry; Retinal Development and Disorders
TL;DR:
Through the light-responsive modification of plasmid-based gene expression systems, optochemical activation of an exogenous fluorescent reporter gene in both tissue culture and a live animal model, as well as light-induced overexpression of an endogenous signaling protein are demonstrated.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2014 journal article
Synthesis of Non-linear Protein Dimers through a Genetically Encoded Thiol-ene Reaction
PLOS ONE, 9(9).
MeSH headings : Alkenes / metabolism; Animals; Fluorescent Dyes / metabolism; Green Fluorescent Proteins / metabolism; Lysine / metabolism; Methanosarcina barkeri / metabolism; Muramidase / metabolism; Myoglobin / metabolism; Protein Multimerization; Sulfhydryl Compounds / metabolism
topics (OpenAlex): Click Chemistry and Applications; Chemical Synthesis and Analysis; Fluorine in Organic Chemistry
TL;DR:
The genetic encoding of a set of alkene lysines is demonstrated using the orthogonal PylRS/PylTCUA pair in Escherichia coli, showing the applicability of this approach in the formation of heterogeneous non-linear fused proteins.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2014 journal article
Thiourea-Based Fluorescent Chemosensors for Aqueous Metal Ion Detection and Cellular Imaging
JOURNAL OF ORGANIC CHEMISTRY, 79(13), 6054–6060.
MeSH headings : Fluorescent Dyes / chemistry; HeLa Cells; Humans; Ions / chemistry; Mercury / analysis; Mercury / chemistry; Metals / analysis; Metals / chemistry; Molecular Imaging; Spectrometry, Fluorescence; Thiourea / chemistry; Water / chemistry
topics (OpenAlex): Molecular Sensors and Ion Detection; Advanced biosensing and bioanalysis techniques; Luminescence and Fluorescent Materials
TL;DR:
It is shown that a thiourea-based chemosensor can be employed for fluorescence microscopy imaging of Hg(2+) ion concentrations in living mammalian cells.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2014 journal article
Two Rapid Catalyst-Free Click Reactions for In Vivo Protein Labeling of Genetically Encoded Strained Alkene/Alkyne Functionalities
BIOCONJUGATE CHEMISTRY, 25(9), 1730–1738.
MeSH headings : Alkenes / chemistry; Alkynes / chemistry; Catalysis; Click Chemistry; Cyclooctanes / chemistry; Escherichia coli Proteins / chemistry; Kinetics; Models, Molecular; Protein Conformation; Proteins / chemistry; Staining and Labeling / methods; Tyrosine / chemistry
topics (OpenAlex): Click Chemistry and Applications; Monoclonal and Polyclonal Antibodies Research; Chemical Synthesis and Analysis
TL;DR:
To apply these “click” reactions for site-specific protein labeling, five tyrosine derivatives that contain a norbornene, trans-cyclooctene, or cyclooctyne entity were genetically encoded into proteins in Escherichia coli using an engineered pyrrolysyl-tRNA synthetase-tRNACUAPyl pair.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2013 journal article
Cellular Delivery and Photochemical Activation of Antisense Agents through a Nucleobase Caging Strategy
ACS CHEMICAL BIOLOGY, 8(10), 2272–2282.
MeSH headings : Base Sequence; Gene Transfer Techniques; HeLa Cells; Humans; Light; Models, Biological; Molecular Structure; Oligonucleotides, Antisense / chemistry; Oligonucleotides, Antisense / genetics; Oligonucleotides, Antisense / metabolism; Polymerase Chain Reaction; tat Gene Products, Human Immunodeficiency Virus / chemistry; tat Gene Products, Human Immunodeficiency Virus / genetics
topics (OpenAlex): RNA Interference and Gene Delivery; Advanced biosensing and bioanalysis techniques; Photochromic and Fluorescence Chemistry
TL;DR:
The conjugation of multiple HIV TAT peptides to a hairpin-protected antisenseAgent through a light-cleavable nucleobase caging group allows for the facile delivery of the antisense agent without a transfection reagent, and photochemical activation offers precise control over gene expression.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2013 journal article
DNA Computation in Mammalian Cells: MicroRNA Logic Operations
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 135(28), 10512–10518.
MeSH headings : Computers, Molecular; DNA / chemistry; DNA / metabolism; HeLa Cells; Humans; MicroRNAs / chemistry; MicroRNAs / metabolism; Photochemical Processes
topics (OpenAlex): Advanced biosensing and bioanalysis techniques; RNA Interference and Gene Delivery; DNA and Nucleic Acid Chemistry
TL;DR:
Nucleic acid logic gates that are functional in a cellular environment and recognize endogenous inputs significantly expand the potential of DNA computation to monitor, image, and respond to cell-specific markers.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2013 journal article
Genetically encoded light-activated transcription for spatiotemporal control of gene expression and gene silencing in mammalian cells
Journal of the American Chemical Society, 135(36), 13433–13439.
MeSH headings : Activating Transcription Factors / genetics; Activating Transcription Factors / metabolism; DNA-Directed RNA Polymerases / genetics; DNA-Directed RNA Polymerases / metabolism; Gene Expression Regulation, Enzymologic / genetics; Gene Silencing; Green Fluorescent Proteins / genetics; Green Fluorescent Proteins / metabolism; HEK293 Cells; Humans; Light; Luciferases / genetics; Luciferases / metabolism; Models, Molecular; Molecular Structure; Photochemical Processes; Protein Engineering; Viral Proteins / genetics; Viral Proteins / metabolism
topics (OpenAlex): Photochromic and Fluorescence Chemistry; bioluminescence and chemiluminescence research; CRISPR and Genetic Engineering
TL;DR:
The photocaging of a synthetic gene network using unnatural amino acid mutagenesis in mammalian cells was demonstrated with an engineered bacteriophage RNA polymerase, demonstrating tight spatial and temporal control.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: NC State University Libraries
Added: August 6, 2018
2013 journal article
MicroRNA miR-122 as a therapeutic target for oligonucleotides and small molecules
Current Medicinal Chemistry, 20(29), 3629–3640.
MeSH headings : Animals; Hepacivirus / drug effects; Hepacivirus / genetics; Hepacivirus / physiology; Humans; Liver Diseases / drug therapy; Liver Diseases / genetics; MicroRNAs / antagonists & inhibitors; MicroRNAs / genetics; Molecular Targeted Therapy / methods; Oligonucleotides, Antisense / genetics; Oligonucleotides, Antisense / therapeutic use; Small Molecule Libraries / pharmacology; Small Molecule Libraries / therapeutic use
topics (OpenAlex): MicroRNA in disease regulation; RNA modifications and cancer; Circular RNAs in diseases
TL;DR:
Various probes have been developed to promote a better understanding of the involvement of miR-122 in liver diseases, including modified antisense agents and small molecule inhibitors, which provide excellent tools to investigate the function and regulation of mi R-122.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: NC State University Libraries
Added: August 6, 2018
2013 journal article
Optochemical control of RNA interference in mammalian cells
NUCLEIC ACIDS RESEARCH, 41(22), 10518–10528.
topics (OpenAlex): Photochromic and Fluorescence Chemistry; Photoreceptor and optogenetics research; RNA Interference and Gene Delivery
TL;DR:
New photocaged guanosine and uridine RNA phosphoramidites are designed, synthesized and site-specifically incorporated into short RNA duplexes and demonstrated in the light-regulation of the expression of an exogenous green fluorescent protein reporter gene and an endogenous target gene, the mitosis motor protein, Eg5.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2012 journal article
DNA Computation: A Photochemically Controlled AND Gate
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 134(8), 3810–3815.
MeSH headings : Base Sequence; Computers, Molecular; DNA / chemistry; Logic; Photochemistry
topics (OpenAlex): Advanced biosensing and bioanalysis techniques; DNA and Nucleic Acid Chemistry; Molecular Sensors and Ion Detection
TL;DR:
A photochemically controlled AND gate was developed through the incorporation of caged thymidine nucleotides into a DNA-based logic gate, using light as the logic inputs, and both spatial control and temporal control were achieved.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2012 journal article
Genetically encoded norbornene directs site-specific cellular protein labelling via a rapid bioorthogonal reaction
NATURE CHEMISTRY, 4(4), 298–304.
MeSH headings : Amino Acids / metabolism; Amino Acyl-tRNA Synthetases / metabolism; Cell Line; Fluorescent Dyes / chemistry; Green Fluorescent Proteins / genetics; Green Fluorescent Proteins / metabolism; Humans; Lysine / analogs & derivatives; Lysine / metabolism; Norbornanes / chemistry; Proteins / genetics; Proteins / metabolism; Rhodamines / chemistry
topics (OpenAlex): Click Chemistry and Applications; Monoclonal and Polyclonal Antibodies Research; Chemical Synthesis and Analysis
TL;DR:
The genetic encoding of a norbornene amino acid using the pyrrolysyl tRNA synthetase/tRNA(CUA) pair in Escherichia coli and mammalian cells and the rapid bioorthogonal site-specific labelling of a protein on the mammalian cell surface is demonstrated.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2012 journal article
High-Throughput Luciferase Reporter Assay for Small-Molecule Inhibitors of MicroRNA Function
JOURNAL OF BIOMOLECULAR SCREENING, 17(6), 822–828.
author keywords: high-throughput assay; cell-based assay; luciferase; microRNA; small-molecule inhibitor
MeSH headings : Antineoplastic Agents / pharmacology; Antiviral Agents / pharmacology; Carcinoma, Hepatocellular / genetics; Carcinoma, Hepatocellular / metabolism; Cell Line, Tumor; Gene Expression; Genes, Reporter; Hepacivirus / metabolism; Hepatitis C / genetics; Hepatitis C / metabolism; High-Throughput Screening Assays / methods; Humans; Liver Neoplasms / genetics; Liver Neoplasms / metabolism; Luciferases, Renilla / genetics; Luciferases, Renilla / metabolism; MicroRNAs / antagonists & inhibitors; MicroRNAs / metabolism; Oligonucleotides, Antisense; Small Molecule Libraries / pharmacology; Transfection
topics (OpenAlex): MicroRNA in disease regulation; RNA Interference and Gene Delivery; Advanced biosensing and bioanalysis techniques
TL;DR:
A high-throughput screen for potential small-molecule regulators of the liver-specific microRNA miR-122, which is involved in hepatocellular carcinoma development and hepatitis C virus infection, is developed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2012 journal article
Hydrogen Peroxide Induced Activation of Gene Expression in Mammalian Cells using Boronate Estrone Derivatives
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 51(36), 9066–9070.
author keywords: biosensors; estrogen receptor; estrone; gene expression; hydrogen peroxide
topics (OpenAlex): Advanced biosensing and bioanalysis techniques; Molecular Sensors and Ion Detection; Marine Biology and Environmental Chemistry
TL;DR:
Keeping the boron out of the ER: A genetic switch was engineered that activates gene expression in the presence of H(2)O(2).
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2012 review
Light-controlled synthetic gene circuits
[Review of ]. CURRENT OPINION IN CHEMICAL BIOLOGY, 16(3-4), 292–299.
MeSH headings : Animals; Cell Line; Gene Regulatory Networks / drug effects; Gene Regulatory Networks / radiation effects; Genetic Engineering / methods; Humans; Light; Photochemical Processes
topics (OpenAlex): bioluminescence and chemiluminescence research; Photoreceptor and optogenetics research; Advanced biosensing and bioanalysis techniques
TL;DR:
Recent advancements in the area of light-controlled synthetic networks are described, which allows for the regulation of intermediate steps of complex dynamic pathways in mammalian cells such as those involved in kinase networks.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2012 journal article
Photocontrol of Tyrosine Phosphorylation in Mammalian Cells via Genetic Encoding of Photocaged Tyrosine
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 134(29), 11912–11915.
MeSH headings : Amino Acyl-tRNA Synthetases / genetics; Escherichia coli / genetics; Escherichia coli / metabolism; HEK293 Cells; Humans; Light; Methanosarcina / enzymology; Methanosarcina / genetics; Mutation; Phosphorylation; Protein Engineering / methods; STAT1 Transcription Factor / genetics; STAT1 Transcription Factor / metabolism; Signal Transduction; Tyrosine / genetics; Tyrosine / metabolism
topics (OpenAlex): RNA and protein synthesis mechanisms; interferon and immune responses; Cytokine Signaling Pathways and Interactions
TL;DR:
By photocaging Tyr701 of STAT1, it is demonstrated that it is possible to photocontrol tyrosine phosphorylation and signal transduction in mammalian cells.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2012 journal article
Regulation of transcription through light-activation and light-deactivation of triplex-forming oligonucleotides in mammalian cells
ACS Chemical Biology, 7(7), 1247–1256.
MeSH headings : DNA / chemistry; DNA / metabolism; HEK293 Cells; Humans; Nucleic Acid Conformation; Oligonucleotides / chemistry; Oligonucleotides / metabolism; Photic Stimulation / methods; Transcription, Genetic / physiology
topics (OpenAlex): Photochromic and Fluorescence Chemistry; DNA and Nucleic Acid Chemistry; Advanced biosensing and bioanalysis techniques
TL;DR:
These are the first examples of light-regulated TFOs and their application in the photochemical activation and deactivation of gene expression and hairpin loop structures were found to significantly increase the efficacy of phosphodiester DNA-based T FOs in tissue culture.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: NC State University Libraries
Added: August 6, 2018
2012 journal article
Spatiotemporal control of microRNA function using light-activated antagomirs
MOLECULAR BIOSYSTEMS, 8(11), 2987–2993.
MeSH headings : Antagomirs; Apoptosis / drug effects; Apoptosis / radiation effects; Cell Differentiation / drug effects; Cell Differentiation / radiation effects; Cell Line, Tumor; Cell Proliferation / drug effects; Cell Proliferation / radiation effects; Humans; Light; MicroRNAs / antagonists & inhibitors; MicroRNAs / metabolism; MicroRNAs / radiation effects; Oligonucleotides / chemistry; Oligonucleotides / pharmacology; Oligonucleotides / radiation effects; Photochemistry; Ultraviolet Rays
topics (OpenAlex): Photochromic and Fluorescence Chemistry; Advanced biosensing and bioanalysis techniques; Luminescence and Fluorescent Materials
TL;DR:
The presented approach enables the precise regulation of miRNA function and miRNA networks with unprecedented spatial and temporal resolution using UV irradiation and can be extended to any miRNA of interest.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2011 journal article
A photoactivatable small-molecule inhibitor for light-controlled spatiotemporal regulation of Rho kinase in live embryos
Development, 139(2), 437–442.
author keywords: Small-molecule inhibitor; Rho kinase; Photocaging; Xenopus
MeSH headings : Animals; Body Patterning / drug effects; Dioxoles / metabolism; Dioxoles / pharmacology; Embryo, Nonmammalian / metabolism; Immunohistochemistry; Indoles / metabolism; Indoles / pharmacology; Mice; NIH 3T3 Cells; Pyridines / metabolism; Pyridines / pharmacology; Ultraviolet Rays; Xenopus laevis / embryology; Xenopus laevis / metabolism; rho-Associated Kinases / antagonists & inhibitors; rho-Associated Kinases / metabolism
topics (OpenAlex): Photochromic and Fluorescence Chemistry; Photoreceptor and optogenetics research; Click Chemistry and Applications
TL;DR:
A novel pharmacological agent that can be light activated to achieve spatiotemporally limited inhibition of Rho kinase activity in vivo is developed and application to the spatially heterogeneous problem of embryonic left-right asymmetry revealed a differential requirement for Rho signaling on the left and right sides of the primitive gut tube.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: August 6, 2018
2011 journal article
Efficacy of C-N Coupling Reactions with a New Multinuclear Copper Complex Catalyst and Its Dissociation into Mononuclear Species
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY, 2011(22), 4154–4159.
author keywords: Reaction mechanisms; Nitrogen heterocycles; C-N coupling; Cross-coupling; Arylation; Copper; Homogeneous catalysis
topics (OpenAlex): Catalytic Cross-Coupling Reactions; Cyclopropane Reaction Mechanisms
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2011 journal article
Genetically encoding an aliphatic diazirine for protein photocrosslinking
CHEMICAL SCIENCE, 2(3), 480–483.
topics (OpenAlex): Click Chemistry and Applications; RNA and protein synthesis mechanisms; Chemical Synthesis and Analysis
TL;DR:
A novel aliphatic diazirine amino acid and its genetically encoded, site-specific incorporation into proteins in bacterial and mammalian cells are reported and efficient photocrosslinking of a test protein is demonstrated in vitro and in vivo.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2011 journal article
Heterotaxin: A TGF-beta Signaling Inhibitor Identified in a Multi-Phenotype Profiling Screen in Xenopus Embryos
CHEMISTRY & BIOLOGY, 18(2), 252–263.
MeSH headings : Animals; Cell Line; Drug Evaluation, Preclinical; Embryo, Nonmammalian / abnormalities; Embryo, Nonmammalian / cytology; Embryo, Nonmammalian / drug effects; Embryo, Nonmammalian / metabolism; Humans; Intracellular Space / drug effects; Intracellular Space / metabolism; Neovascularization, Physiologic / drug effects; Phenotype; Pyridines / chemistry; Pyridines / pharmacology; Signal Transduction / drug effects; Stereoisomerism; Structure-Activity Relationship; Transforming Growth Factor beta / metabolism; Xenopus laevis
topics (OpenAlex): Congenital heart defects research; Developmental Biology and Gene Regulation
TL;DR:
A small molecule screen for left-right asymmetry phenotypes in Xenopus embryos is performed and a pyridine analog, heterotaxin, is discovered, which disrupts both cardiovascular and digestive organ laterality and inhibits TGF-β-dependent left- right asymmetric gene expression.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2011 journal article
Light Regulation of Protein Dimerization and Kinase Activity in Living Cells Using Photocaged Rapamycin and Engineered FKBP
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 133(3), 420–423.
MeSH headings : Dimerization; Focal Adhesion Protein-Tyrosine Kinases / metabolism; HEK293 Cells; Humans; Light; Models, Molecular; Molecular Dynamics Simulation; Sirolimus / chemistry; Tacrolimus Binding Proteins / chemistry; Tacrolimus Binding Proteins / metabolism
topics (OpenAlex): Photochromic and Fluorescence Chemistry; Click Chemistry and Applications; Neurobiology and Insect Physiology Research
TL;DR:
A new system for light-induced protein dimerization in living cells is developed using a photocaged analogue of rapamycin together with an engineered Rapamycin binding domain and focal adhesion kinase as a target, which demonstrated successful light-mediated regulation of protein interaction and localization in live cells.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2011 journal article
Light-Activated Gene Editing with a Photocaged Zinc-Finger Nuclease
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 50(30), 6839–6842.
author keywords: caged compounds; gene technology; light-activation; mutagenesis; zinc-finger nucleases
MeSH headings : Endonucleases / genetics; Endonucleases / metabolism; Escherichia coli / genetics; HEK293 Cells; Humans; Models, Molecular; Mutagenesis; Photochemical Processes; Protein Engineering; Tyrosine / genetics; Ultraviolet Rays; Zinc Fingers
topics (OpenAlex): CRISPR and Genetic Engineering; Photochromic and Fluorescence Chemistry; Advanced biosensing and bioanalysis techniques
TL;DR:
A family of artificial restriction enzymes, namely zinc-finger nucleases (ZFN), has been developed to sequence-selectively achieve dsDNA scission and have since emerged as important and widely recognized tools for the genetic modification of cells, model organisms, and possibly humans to investigate gene function and to treat genetic disorders.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2011 journal article
Light-Activated Kinases Enable Temporal Dissection of Signaling Networks in Living Cells
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 133(7), 2124–2127.
MeSH headings : Animals; Catalytic Domain; Humans; Light; Mitogen-Activated Protein Kinase Kinases / chemistry; Mitogen-Activated Protein Kinase Kinases / pharmacology; Models, Molecular; Phosphorylation; Phosphotransferases / chemistry; Phosphotransferases / pharmacology; Signal Transduction / drug effects
topics (OpenAlex): Photoreceptor and optogenetics research; Photochromic and Fluorescence Chemistry; bioluminescence and chemiluminescence research
TL;DR:
Time-lapse microscopy allowed us to precisely characterize the kinetics of elementary steps in the signaling cascade and provided insight into adaptive feedback and rate-determining processes in the pathway.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2011 journal article
Photochemical Control of DNA Decoy Function Enables Precise Regulation of Nuclear Factor kappa B Activity
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 133(33), 13176–13182.
MeSH headings : Alkaline Phosphatase / genetics; Gene Expression Regulation / radiation effects; Light; Methods; NF-kappa B / metabolism; Oligodeoxyribonucleotides / radiation effects; Photochemical Processes; Transcription, Genetic / drug effects
topics (OpenAlex): Photochromic and Fluorescence Chemistry; Bioactive Compounds and Antitumor Agents; Synthesis of Indole Derivatives
TL;DR:
This is the first example of a caged DNA decoy for the photochemical regulation of gene expression in mammalian cells and represents an important addition to the toolbox of light-controlled gene regulatory agents.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2011 journal article
Photochemical control of bacterial signal processing using a light-activated erythromycin
MOLECULAR BIOSYSTEMS, 7(9), 2554–2557.
MeSH headings : Anti-Bacterial Agents / pharmacology; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Drug Resistance, Bacterial / drug effects; Drug Resistance, Bacterial / genetics; Erythromycin / pharmacology; Escherichia coli / genetics; Escherichia coli / metabolism; Escherichia coli / radiation effects; Exoribonucleases / genetics; Exoribonucleases / metabolism; Light; Photochemistry / methods
topics (OpenAlex): Photoreceptor and optogenetics research; bioluminescence and chemiluminescence research; Photochromic and Fluorescence Chemistry
TL;DR:
This work engineered the MphR(A)/promoter system to enable photochemical activation of gene expression in E. coli, and applied this photochemical gene switch to the construction of a light-triggered logic gate, aLight-controlled band-pass filter, as well as spatial and temporal control of geneexpression.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2011 journal article
Stabilization and Photochemical Regulation of Antisense Agents through PEGylation
BIOCONJUGATE CHEMISTRY, 22(10), 2136–2142.
MeSH headings : Animals; DNA, Antisense / chemical synthesis; DNA, Antisense / chemistry; DNA, Antisense / genetics; DNA, Antisense / metabolism; Gene Expression Regulation; HEK293 Cells; Humans; Luminescent Proteins / genetics; Mice; NIH 3T3 Cells; Nucleic Acid Denaturation; Photolysis; Polyethylene Glycols / chemistry; Ribonuclease H / metabolism; Ultraviolet Rays
topics (OpenAlex): DNA and Nucleic Acid Chemistry; RNA Interference and Gene Delivery; Advanced biosensing and bioanalysis techniques
TL;DR:
By employing a photocleavable linker between the PEG group and the antisense agent, the paper was able to achieve light-induced deactivation of antisense activity and pave the way for precise regulation of gene expression in biological systems.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2011 journal article
Synthesis of the Pyridine Core of Cyclothiazomycin
ORGANIC LETTERS, 13(16), 4352–4355.
MeSH headings : Anti-Bacterial Agents / chemical synthesis; Molecular Structure; Peptides, Cyclic / chemistry; Pyridines / chemical synthesis; Thiazoles / chemistry
topics (OpenAlex): Chemical Synthesis and Analysis; Synthesis and Biological Evaluation
TL;DR:
A highly convergent synthesis of the pyridine core of the thiopeptide antibiotic cyclothiazomycin has been developed based on a [2-2+2] cyclotrimerization key step.
(via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018
2011 journal article
The Human Mitochondrial tRNA(Met): Structure/Function Relationship of a Unique Modification in the Decoding of Unconventional Codons
JOURNAL OF MOLECULAR BIOLOGY, 406(2), 257–274.
author keywords: 5-formylcytidine; NMR structure; mitochondrial translation; codon expansion; near cognate decoding
MeSH headings : Animals; Anticodon / chemistry; Anticodon / genetics; Base Pairing; Base Sequence; Cattle; Cytidine / analogs & derivatives; Cytidine / chemistry; Cytidine / genetics; Escherichia coli / genetics; Humans; Mitochondria / chemistry; Mitochondria / genetics; Molecular Sequence Data; RNA, Transfer, Met / chemistry; RNA, Transfer, Met / genetics; Ribosomes / chemistry; Ribosomes / genetics; Structure-Activity Relationship
topics (OpenAlex): RNA and protein synthesis mechanisms; RNA modifications and cancer; Genomics and Phylogenetic Studies
TL;DR:
The physicochemical properties of this one modification, f(5)C(34), expand codon recognition from the traditional AUG to the non-traditional, synonymous codons AUU and AUC as well as AUA, in the reassignment of universal codons in the mitochondria.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2010 journal article
Activation and Deactivation of DNAzyme and Antisense Function with Light for the Photochemical Regulation of Gene Expression in Mammalian Cells
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 132(17), 6183–6193.
MeSH headings : Base Sequence; Cell Line; DNA / chemistry; DNA / metabolism; DNA, Catalytic / metabolism; DNA, Catalytic / radiation effects; Enzyme Activation; Gene Expression / radiation effects; Gene Expression Regulation; Humans; Light; Molecular Sequence Data; Nucleic Acid Conformation; Oligodeoxyribonucleotides, Antisense / metabolism; Oligodeoxyribonucleotides, Antisense / radiation effects; Photochemical Processes; RNA / metabolism
topics (OpenAlex): Advanced biosensing and bioanalysis techniques; DNA and Nucleic Acid Chemistry; Photochromic and Fluorescence Chemistry
TL;DR:
The application of light-activated and light-deactivated antisense agents to the regulation of gene function in mammalian cells represents the first example of gene silencing antisense agent that can be turned on and turned off in mammalian tissue culture.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2010 journal article
Expanding the Genetic Code of Yeast for Incorporation of Diverse Unnatural Amino Acids via a Pyrrolysyl-tRNA Synthetase/tRNA Pair
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 132(42), 14819–14824.
MeSH headings : Amino Acids / metabolism; Amino Acyl-tRNA Synthetases / metabolism; Genetic Code; RNA, Fungal / metabolism; RNA, Transfer / metabolism; Saccharomyces cerevisiae / enzymology; Saccharomyces cerevisiae / genetics
topics (OpenAlex): RNA and protein synthesis mechanisms; Chemical Synthesis and Analysis; Click Chemistry and Applications
TL;DR:
The discovery of a simple system through which variant pyrrolysyl-tRNA synthetase/tRNACUAPyl pairs created in Escherichia coli can be used to expand the genetic code of Saccharomyces cerevisiae is reported.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2010 journal article
Generating Permissive Site-Specific Unnatural Aminoacyl-tRNA Synthetases
BIOCHEMISTRY, 49(8), 1667–1677.
MeSH headings : Amino Acids / chemistry; Amino Acids / metabolism; Amino Acyl-tRNA Synthetases / chemistry; Amino Acyl-tRNA Synthetases / genetics; Amino Acyl-tRNA Synthetases / metabolism; Fluorine / chemistry; Green Fluorescent Proteins / chemistry; Green Fluorescent Proteins / genetics; Green Fluorescent Proteins / metabolism; Magnetic Resonance Spectroscopy; Mass Spectrometry; Molecular Structure; Protein Engineering; Substrate Specificity
topics (OpenAlex): RNA and protein synthesis mechanisms; Mass Spectrometry Techniques and Applications; RNA modifications and cancer
TL;DR:
Methods for quickly and easily assessing the permissivity of existing UaaRSs and for generating permissive UAARSs are outlined, whose relaxed substrate recognition properties allow them to incorporate multiple unnatural amino acids (but not natural amino acids), are outlined.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2010 journal article
Genetically encoded photocontrol of protein localization in mammalian cells
Journal of the American Chemical Society, 132(12), 4086-.
Source: NC State University Libraries
Added: August 6, 2018
2010 journal article
Improved Synthesis of the Two-Photon Caging Group 3-Nitro-2-Ethyldibenzofuran and Its Application to a Caged Thymidine Phosphoramidite
ORGANIC LETTERS, 12(5), 916–919.
MeSH headings : Benzofurans / chemical synthesis; Benzofurans / chemistry; DNA / chemical synthesis; DNA / chemistry; Organophosphorus Compounds / chemistry; Photons; Thymidine Monophosphate / analogs & derivatives; Thymidine Monophosphate / chemistry
topics (OpenAlex): Photochromic and Fluorescence Chemistry; Photoreceptor and optogenetics research; Porphyrin and Phthalocyanine Chemistry
TL;DR:
A new and efficient route to the recently reported 3-nitro-2-ethyldibenzofuran caging group was developed and its installation on a thymidine phosphoramidite is described.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2010 journal article
Photocaged Morpholino Oligomers for the Light-Regulation of Gene Function in Zebrafish and Xenopus Embryos
Journal of the American Chemical Society, 132(44), 15644–15650.
MeSH headings : Animals; Cells, Cultured; Embryo, Nonmammalian / metabolism; Gene Expression; Morpholines / chemistry; Oligonucleotides / genetics; Oligonucleotides / metabolism; Ultraviolet Rays; Xenopus laevis / genetics; Xenopus laevis / metabolism; Zebrafish / genetics; Zebrafish / metabolism
topics (OpenAlex): Photochromic and Fluorescence Chemistry; RNA Interference and Gene Delivery; Advanced biosensing and bioanalysis techniques
TL;DR:
A novel and efficient synthetic route for incorporating photocaged monomeric building blocks directly into morpholino oligomers is reported and the utility of these caged morpholinos in the light-activated control of gene function in both cell culture and living embryos is demonstrated.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: August 6, 2018
2010 journal article
Photocaged T7 RNA Polymerase for the Light Activation of Transcription and Gene Function in Pro- and Eukaryotic Cells
CHEMBIOCHEM, 11(7), 972–977.
author keywords: amino acids; caged proteins; light activation; polymerases; RNA
MeSH headings : Catalytic Domain; Cell Line; DNA-Directed RNA Polymerases / genetics; DNA-Directed RNA Polymerases / metabolism; Escherichia coli / enzymology; Humans; Luciferases / genetics; Luciferases / metabolism; Models, Molecular; Photochemical Processes; Transcriptional Activation; Tyrosine / chemistry; Ultraviolet Rays; Viral Proteins / genetics; Viral Proteins / metabolism
topics (OpenAlex): Photochromic and Fluorescence Chemistry; bioluminescence and chemiluminescence research; DNA and Nucleic Acid Chemistry
TL;DR:
The ability to achieve spatiotemporal regulation of orthogonal RNA synthesis enables the precise dissection and manipulation of a wide range of cellular events, including gene function.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2010 journal article
Photocleavable Polyethylene Glycol for the Light-Regulation of Protein Function
BIOCONJUGATE CHEMISTRY, 21(8), 1404–1407.
MeSH headings : Crystallography, X-Ray; Models, Molecular; Molecular Structure; Muramidase / chemistry; Muramidase / metabolism; Photochemical Processes; Polyethylene Glycols / chemical synthesis; Polyethylene Glycols / chemistry; Stereoisomerism; Ultraviolet Rays
topics (OpenAlex): Click Chemistry and Applications; Photochromic and Fluorescence Chemistry; Chemical Synthesis and Analysis
TL;DR:
The synthesis of a photocleavable PEG reagent (PhotoPEG) and its application to the light-regulation of enzyme activity is reported.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2010 journal article
Principles and Applications of the Photochemical Control of Cellular Processes
CHEMBIOCHEM, 11(1), 47–53.
author keywords: biological activity; cage compounds; chemical biology; light regulation; photochemistry
MeSH headings : Animals; Biological Phenomena / radiation effects; DNA / chemistry; Doxycycline / chemistry; Embryo, Mammalian; Mice; Phosphorothioate Oligonucleotides / chemistry; Photochemical Processes; Ultraviolet Rays
topics (OpenAlex): Photochromic and Fluorescence Chemistry; Photoreceptor and optogenetics research; Click Chemistry and Applications
TL;DR:
The basic concepts and requirements of controlling biological (especially cellular) function with light, including photochemical regulation of biologically active molecules, are focused on.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
4. Quality Education
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2010 review
Recent advances in the photochemical control of protein function
[Review of ]. TRENDS IN BIOTECHNOLOGY, 28(9), 468–475.
MeSH headings : Animals; Biotechnology / methods; Cell Line; Humans; Ion Channels / chemistry; Ion Channels / genetics; Ion Channels / physiology; Light; Models, Molecular; Photochemical Processes; Photochemistry / methods; Proteins / chemistry; Proteins / genetics; Proteins / physiology
topics (OpenAlex): Photochromic and Fluorescence Chemistry; Light effects on plants; bioluminescence and chemiluminescence research
TL;DR:
This review article summarizes the most recent advances in light-mediated regulation of protein function and its application in a cellular context and examples of the photochemical control of proteinfunction through the application of genetically engineered natural-light receptors are presented.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2010 journal article
Reversible light switching of cell signalling by genetically encoded protein dimerization
Chembiochem, 11(3), 301–303.
MeSH headings : Animals; Bacterial Proteins / chemistry; Bacterial Proteins / metabolism; Cell Membrane / chemistry; Cell Membrane / metabolism; Light; Luminescent Proteins / chemistry; Luminescent Proteins / metabolism; Mice; NIH 3T3 Cells; Phycobilins / chemistry; Phycocyanin / chemistry; Phytochrome B / chemistry; Phytochrome B / genetics; Phytochrome B / metabolism; Protein Multimerization; Protein Structure, Tertiary; Signal Transduction
topics (OpenAlex): Photochromic and Fluorescence Chemistry; Light effects on plants; Photoreceptor and optogenetics research
TL;DR:
This work has shown that a light-switchable transcriptional activator was constructed from a protein–protein interaction module in the phytochrome signalling network of the plant Arabidopsis thaliana, which enables the application of light toward the genetically encoded, reversible photoregulation of a cellular event.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Source: NC State University Libraries
Added: August 6, 2018
2010 journal article
Site-Specific Incorporation of Fluorotyrosines into Proteins in Escherichia coli by Photochemical Disguise
BIOCHEMISTRY, 49(8), 1557–1559.
MeSH headings : Escherichia coli / genetics; Escherichia coli / metabolism; Escherichia coli Proteins / chemistry; Escherichia coli Proteins / genetics; Escherichia coli Proteins / metabolism; Molecular Structure; Protein Engineering / methods; Protein Structure, Secondary; Tyrosine / analogs & derivatives; Tyrosine / chemical synthesis; Tyrosine / chemistry; Tyrosine / metabolism
topics (OpenAlex): Click Chemistry and Applications; RNA and protein synthesis mechanisms; Chemical Synthesis and Analysis
TL;DR:
This work genetically encoded the unnatural amino acids o-nitrobenzyl-2-fluorotyrosine, -3-fluorschmidtine, and -2,6-difluorotYrosine in Escherichia coli, effectively preventing global incorporation of fluorotyosine into proteins.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2010 journal article
Small Molecule Modifiers of MicroRNA miR-122 Function for the Treatment of Hepatitis C Virus Infection and Hepatocellular Carcinoma
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 132(23), 7976–7981.
MeSH headings : Apoptosis / drug effects; Base Sequence; Carcinoma, Hepatocellular / drug therapy; Carcinoma, Hepatocellular / genetics; Carcinoma, Hepatocellular / pathology; Enoxacin / pharmacology; HeLa Cells; Hepatitis C / drug therapy; Hepatitis C / genetics; Humans; Liver Neoplasms / drug therapy; Liver Neoplasms / genetics; Liver Neoplasms / pathology; MicroRNAs / antagonists & inhibitors; MicroRNAs / genetics; MicroRNAs / metabolism; Oligonucleotides, Antisense / genetics; Oligonucleotides, Antisense / pharmacology; Virus Replication / drug effects; Virus Replication / genetics
topics (OpenAlex): MicroRNA in disease regulation; Circular RNAs in diseases; RNA modifications and cancer
TL;DR:
The authors' small molecule inhibitors reduce viral replication in liver cells and represent a new approach to the treatment of HCV infections and small molecule activation of miR-122 in liver cancer cells selectively induced apoptosis through caspase activation, thus having implications in cancer chemotherapy.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2010 review
Small Molecule Modifiers of the microRNA and RNA Interference Pathway
[Review of ]. AAPS JOURNAL, 12(1), 51–60.
author keywords: cancer; microRNA; RNA; RNA interference; small molecule
MeSH headings : Animals; Humans; MicroRNAs / antagonists & inhibitors; MicroRNAs / biosynthesis; RNA Interference / drug effects; RNA, Small Interfering / biosynthesis
topics (OpenAlex): MicroRNA in disease regulation; RNA modifications and cancer; RNA Interference and Gene Delivery
TL;DR:
The activation of tumor-suppressive miRNAs and the inhibition of oncogenicMiRNAs by small molecules have the potential to provide a fundamentally new approach for the development of cancer therapeutics.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2010 journal article
Total Synthesis of Cryptoacetalide
JOURNAL OF ORGANIC CHEMISTRY, 75(15), 5355–5358.
MeSH headings : Chromatography, High Pressure Liquid; Diterpenes / chemical synthesis; Magnetic Resonance Spectroscopy; Mass Spectrometry; Molecular Structure
topics (OpenAlex): Synthetic Organic Chemistry Methods; Biological Activity of Diterpenoids and Biflavonoids; Advanced Synthetic Organic Chemistry
TL;DR:
The first total synthesis of the tetracyclic terpene natural product cryptoacetalide is reported, with key steps a microwave-mediated cyclo-trimerization reaction to construct the central benzene ring, and a light-mediated radical cyclization to assemble the spiro-ketal moiety.
(via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018
2010 journal article
Tricyclic Alkaloid Core Structures Assembled by a Cyclotrimerization-Coupled Intramolecular Nucleophilic Substitution Reaction
ORGANIC LETTERS, 12(6), 1288–1291.
MeSH headings : Alkaloids / chemical synthesis; Alkaloids / chemistry; Cyclization; Microwaves; Molecular Structure; Pyridines / chemistry; Stereoisomerism
topics (OpenAlex): Chemical synthesis and alkaloids; Alkaloids: synthesis and pharmacology; Phenothiazines and Benzothiazines Synthesis and Activities
TL;DR:
A facile approach to tricyclic alkaloid core structures was developed by sequencing a pyridine-forming [2 + 2 + 2] cyclotrimerization reaction with an intramolecular nucleophilic substitution.
(via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018
2009 journal article
A Light-Activated DNA Polymerase
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 48(32), 5950–5953.
author keywords: DNA polymerases; mutagenesis; non-natural amino acids; photoactivation; polymerase chain reaction
MeSH headings : Catalytic Domain; Mutation; Nitrobenzenes / chemistry; Photolysis; Polymerase Chain Reaction; Taq Polymerase / chemistry; Taq Polymerase / metabolism; Tyrosine / chemistry; Ultraviolet Rays
topics (OpenAlex): Photochromic and Fluorescence Chemistry; DNA and Nucleic Acid Chemistry; Advanced biosensing and bioanalysis techniques
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2009 journal article
Development of a Robust and High Throughput Method for Profiling N-Linked Glycans Derived from Plasma Glycoproteins by NanoLC-FTICR Mass Spectrometry
JOURNAL OF PROTEOME RESEARCH, 8(7), 3764–3770.
MeSH headings : Biomarkers, Tumor / metabolism; Blood Proteins / chemistry; Chromatography, Liquid / methods; Detergents / pharmacology; Glycoproteins / chemistry; Humans; Mass Spectrometry / methods; Microwaves; Peptides / chemistry; Polysaccharides / chemistry; Proteome; Proteomics / methods; Spectroscopy, Fourier Transform Infrared / methods; Time Factors
topics (OpenAlex): Glycosylation and Glycoproteins Research; Carbohydrate Chemistry and Synthesis; Enzyme Structure and Function
TL;DR:
A step-by-step protocol describing a method for N-linked glycan profiling of plasma glycoproteins by nanoflow liquid chromatography Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) is presented.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2009 journal article
Genetic Encoding and Labeling of Aliphatic Azides and Alkynes in Recombinant Proteins via a Pyrrolysyl-tRNA Synthetase/tRNA(CUA) Pair and Click Chemistry
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 131(25), 8720-+.
MeSH headings : Alkynes / chemistry; Alkynes / metabolism; Amino Acyl-tRNA Synthetases / genetics; Amino Acyl-tRNA Synthetases / metabolism; Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Azides / chemistry; Azides / metabolism; Escherichia coli / genetics; Escherichia coli Proteins / chemistry; Escherichia coli Proteins / genetics; Escherichia coli Proteins / metabolism; Lysine / chemistry; Lysine / genetics; Lysine / metabolism; Methanosarcina barkeri / enzymology; Myoglobin / chemistry; Myoglobin / genetics; Myoglobin / metabolism; Protein Engineering / methods; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism
topics (OpenAlex): Click Chemistry and Applications; RNA and protein synthesis mechanisms; Chemical Synthesis and Analysis
TL;DR:
It is demonstrated that an orthogonal Methanosarcina barkeri MS pyrrolysyl-tRNA synthetase/tRNA(CUA) pair directs the efficient, site-specific incorporation of N6-[(2-propynyloxy)carbonyl]-L-lysine, containing a carbon-carbon triple bond, into recombinant proteins in Escherichia coli.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2009 review
Light activation as a method of regulating and studying gene expression
[Review of ]. CURRENT OPINION IN CHEMICAL BIOLOGY, 13(5-6), 678–686.
MeSH headings : Animals; Gene Expression Regulation / drug effects; Gene Expression Regulation / radiation effects; Humans; Light; Oligonucleotides / genetics; Oligonucleotides / metabolism; Proteins / metabolism
topics (OpenAlex): Photochromic and Fluorescence Chemistry; bioluminescence and chemiluminescence research; Photoreceptor and optogenetics research
TL;DR:
The majority of the examples employ a classical 'caging technology', through the chemical installation of a light-removable protecting group on the biological molecule (small molecule, oligonucleotide, or protein) of interest and rendering it inactive.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2009 journal article
Light-Activated Cre Recombinase as a Tool for the Spatial and Temporal Control of Gene Function in Mammalian Cells
ACS CHEMICAL BIOLOGY, 4(6), 441–445.
MeSH headings : Binding Sites; Biocatalysis / radiation effects; Cells, Cultured; Cloning, Molecular; DNA / chemistry; DNA / metabolism; Genetic Engineering / methods; Humans; Integrases / chemistry; Integrases / metabolism; Models, Molecular; Nitrobenzenes / chemistry; Nitrobenzenes / pharmacology; Recombination, Genetic / radiation effects; Time Factors; Ultraviolet Rays
topics (OpenAlex): Photochromic and Fluorescence Chemistry; Photoreceptor and optogenetics research; Light effects on plants
TL;DR:
Tight spatio-temporal control over DNA recombination is achieved by the installation of a light-responsive o-nitrobenzyl caging group directly in the catalytic site of Cre, inhibiting its activity.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2009 journal article
Light-activation of gene function in mammalian cells via ribozymes
Chem. Commun., (5), 568–570.
MeSH headings : Cell Line; Gene Expression / radiation effects; Humans; RNA, Catalytic / chemistry; RNA, Catalytic / metabolism; RNA, Messenger / metabolism; Toyocamycin / chemistry; Toyocamycin / radiation effects; Ultraviolet Rays
topics (OpenAlex): Photochromic and Fluorescence Chemistry; Photoreceptor and optogenetics research; Supramolecular Self-Assembly in Materials
TL;DR:
A ribozyme based gene control element enabled the spatio-temporal regulation of gene function in mammalian cell culture with light.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: August 6, 2018
2009 journal article
Open-Vessel Microwave-Mediated [2+2+2]-Cyclotrimerization Reactions
SYNTHESIS-STUTTGART, (22), 3785–3790.
author keywords: [2+2+2] cyclotrimerization; alkynes; microwave; bicyclic compounds; transition metal catalysis
topics (OpenAlex): Catalytic Alkyne Reactions; Catalysis for Biomass Conversion
Source: Web Of Science
Added: August 6, 2018
2009 journal article
Photochemical Regulation of Restriction Endonuclease Activity
CHEMBIOCHEM, 10(10), 1612–1616.
author keywords: cage compounds; DNA cleavage; DNA enzymes; photochemistry
MeSH headings : Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Base Sequence; Benzodioxoles / chemistry; DNA / chemistry; DNA / radiation effects; DNA Cleavage; DNA Restriction Enzymes / genetics; DNA Restriction Enzymes / metabolism; Deoxyribonuclease BamHI / genetics; Deoxyribonuclease BamHI / metabolism; Deoxyribonuclease EcoRI / genetics; Deoxyribonuclease EcoRI / metabolism; Deoxyribonucleases, Type II Site-Specific / genetics; Deoxyribonucleases, Type II Site-Specific / metabolism; Substrate Specificity; Thymidine / analogs & derivatives; Thymidine / chemistry; Ultraviolet Rays
topics (OpenAlex): Photochromic and Fluorescence Chemistry; bioluminescence and chemiluminescence research; Photoreceptor and optogenetics research
TL;DR:
Photolabile protecting groups have been installed on DNA substrates and have been demonstrated to inhibit restriction endonuclease activity until removed by UV light irradiation, but these groups do not appear to dramatically affect initial binding of the enzyme to the DNA substrate, but rather prevent recognition of the specific cleavage site.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2009 journal article
Restriction enzyme-free mutagenesis via the light regulation of DNA polymerization
Nucleic Acids Research, 37(8).
Source: NC State University Libraries
Added: August 6, 2018
2009 journal article
The effect of microwave irradiation on DNA hybridization
ORGANIC & BIOMOLECULAR CHEMISTRY, 7(12), 2506–2508.
MeSH headings : Base Sequence; DNA / chemistry; DNA / genetics; DNA / radiation effects; Microwaves; Nucleic Acid Denaturation / radiation effects; Nucleic Acid Hybridization / radiation effects; Spectrometry, Fluorescence; Transition Temperature
topics (OpenAlex): Microwave-Assisted Synthesis and Applications; DNA and Nucleic Acid Chemistry; Bacteriophages and microbial interactions
TL;DR:
Observations indicate a specific interaction of microwaves with DNA, and have important implications in the chemical or enzymatic processing of DNA under microwave heating.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2008 journal article
A cyclotrimerization route to cannabinoids
ORGANIC LETTERS, 10(11), 2195–2198.
MeSH headings : Alkynes / chemistry; Cannabinoids / chemical synthesis; Cannabinoids / chemistry; Cyclization; Nitriles / chemistry
topics (OpenAlex): Cannabis and Cannabinoid Research; Synthesis of Indole Derivatives; Chemical synthesis and alkaloids
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2008 journal article
A general approach to triphenylenes and azatriphenylenes: total synthesis of dehydrotylophorine and tylophorine
CHEMICAL COMMUNICATIONS, (39), 4750–4752.
MeSH headings : Alkaloids / chemical synthesis; Alkaloids / chemistry; Alkaloids / radiation effects; Aza Compounds / chemical synthesis; Aza Compounds / chemistry; Aza Compounds / radiation effects; Chrysenes / chemical synthesis; Chrysenes / chemistry; Chrysenes / radiation effects; Cyclization; Indolizines / chemical synthesis; Indolizines / chemistry; Indolizines / radiation effects; Microwaves; Molecular Structure; Phenanthrenes / chemical synthesis; Phenanthrenes / chemistry; Phenanthrenes / radiation effects; Stereoisomerism
topics (OpenAlex): Phytochemistry and biological activities of Ficus species; Phytochemical compounds biological activities; Carbohydrate Chemistry and Synthesis
TL;DR:
A convergent and flexible synthesis of substituted triphenylenes, azatriphenylene, and the cytotoxic alkaloids dehydrotylophorine and tylophOrine has been developed.
(via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018
2008 journal article
Gene Silencing in Mammalian Cells with Light-Activated Antisense Agents
ChemBioChem, 9(18), 2937–2940.
author keywords: Antisense agents; caged compounds; DNA; gene regulation; RNA
MeSH headings : Animals; Base Sequence; Gene Silencing; Genes, Reporter; Light; Luciferases, Renilla / chemistry; Mice; NIH 3T3 Cells; Oligonucleotides, Antisense / chemistry; Oligonucleotides, Antisense / metabolism; Oligonucleotides, Antisense / radiation effects; Phosphorothioate Oligonucleotides / chemistry; Phosphorothioate Oligonucleotides / metabolism; Transfection
topics (OpenAlex): Photochromic and Fluorescence Chemistry; bioluminescence and chemiluminescence research; Advanced biosensing and bioanalysis techniques
TL;DR:
Detailed knowledge of the external regulation of gene func-tion is a fundamental necessity in order to annotate sequencedgenomes and to understand biological processes in single cells and multicellular organisms.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: August 6, 2018
2008 journal article
Light-activated deoxyguanosine: photochemical regulation of peroxidase activity
MOLECULAR BIOSYSTEMS, 4(6), 508–511.
MeSH headings : Biocatalysis / radiation effects; DNA, Catalytic / chemical synthesis; DNA, Catalytic / chemistry; DNA, Catalytic / radiation effects; Deoxyguanosine / chemical synthesis; Deoxyguanosine / chemistry; Deoxyguanosine / metabolism; Enzyme Activation / radiation effects; Molecular Structure; Organophosphorus Compounds / chemical synthesis; Organophosphorus Compounds / chemistry; Organophosphorus Compounds / metabolism; Peroxidase / metabolism; Photochemistry; Time Factors; Ultraviolet Rays
topics (OpenAlex): Advanced biosensing and bioanalysis techniques; Photochromic and Fluorescence Chemistry; Nanoplatforms for cancer theranostics
TL;DR:
Photochemical activation of a deoxyribozyme with peroxidase activity was achieved by the synthesis and incorporation of a caged deoxyguanosine.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2008 journal article
Light-regulated RNA-small molecule interactions
CHEMBIOCHEM, 9(8), 1225–1228.
author keywords: aptamer; light; riboswitch; RNA; spiropyran
MeSH headings : Aptamers, Nucleotide / chemistry; Aptamers, Nucleotide / isolation & purification; Benzopyrans / chemistry; Indoles / chemistry; Light; Molecular Structure; Nitro Compounds / chemistry; Photochemistry; Pyrimidinones / chemistry; RNA / chemistry; Substrate Specificity; Surface Plasmon Resonance
topics (OpenAlex): Photochromic and Fluorescence Chemistry; Advanced biosensing and bioanalysis techniques; Photoreceptor and optogenetics research
TL;DR:
The development of light-regulated processes represents a noninvasive means to exert a high level of spatial and temporal control over a chemical or biological system and allows for the irreversible activation or deactivation of the system under study, thus limiting the scope of its utility.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2008 journal article
Light-triggered polymerase chain reaction
CHEMICAL COMMUNICATIONS, (4), 462–464.
MeSH headings : Base Sequence; DNA / chemistry; DNA Primers; Light; Photochemistry; Polymerase Chain Reaction / methods
topics (OpenAlex): Photochromic and Fluorescence Chemistry; DNA and Nucleic Acid Chemistry; Click Chemistry and Applications
TL;DR:
Photochemical control of the polymerase chain reaction has been achieved through the incorporation of light-triggered nucleotides into DNA.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2008 journal article
Microwave activation of enzymatic catalysis
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 130(31), 10048-+.
MeSH headings : Carboxylesterase / metabolism; Catalysis; Enzymes / metabolism; Hot Temperature; Kinetics; Microwaves; Pyrococcus furiosus / enzymology; Sulfolobus solfataricus / enzymology; Thermotoga maritima / enzymology; alpha-Galactosidase / metabolism; beta-Glucosidase / metabolism
topics (OpenAlex): Microwave-Assisted Synthesis and Applications; Chemical Synthesis and Analysis; Asymmetric Hydrogenation and Catalysis
TL;DR:
This is the first illustration of a specific microwave effect in enzymatic catalysis, used to regulate biocatalysis, in a microwave reactor.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2008 journal article
Microwave-assisted synthesis of unnatural amino acids
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 18(20), 5478–5480.
author keywords: microwave; microwave synthesis; amino acid; unnatural amino acid; peptide synthesis; protein synthesis; unnatural amino acid; mutagenesis
MeSH headings : Amino Acids / chemical synthesis; Chemistry, Pharmaceutical / methods; Drug Design; Magnetic Resonance Spectroscopy; Microwaves; Models, Chemical; Mutagenesis; Peptides / chemistry; Proteins / chemistry; Temperature
topics (OpenAlex): Chemical Synthesis and Analysis; Carbohydrate Chemistry and Synthesis; Microwave-Assisted Synthesis and Applications
TL;DR:
Microwave irradiation has been proven to be a useful tool in the rapid assembly of racemic unnatural amino acids in only two steps and its commercial availability and the high yields and high purities of the final amino acid products.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2008 journal article
Microwave-mediated Nickel-catalyzed cyclotrimerization reactions: Total synthesis of illudinine
JOURNAL OF ORGANIC CHEMISTRY, 73(1), 342–345.
MeSH headings : Catalysis; Cyclization; Microwaves; Molecular Structure; Nickel / chemistry; Nickel / radiation effects; Organometallic Compounds / chemistry; Organometallic Compounds / radiation effects; Sesquiterpenes / chemical synthesis; Sesquiterpenes / chemistry; Sesquiterpenes / radiation effects; Stereoisomerism
topics (OpenAlex): Multicomponent Synthesis of Heterocycles; Microwave-Assisted Synthesis and Applications; Synthesis and Biological Evaluation
TL;DR:
The developed microwave-mediated Ni-catalyzed cyclotrimerization reaction was employed as the key step in a concise synthesis of the isoquinoline natural product illudinine, the first example of a Ni-Catalyzedcyclotriming reaction in total synthesis.
(via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018
2008 journal article
Phenanthridine synthesis via [2+2+2] cyclotrimerization reactions
ORGANIC & BIOMOLECULAR CHEMISTRY, 6(2), 263–265.
MeSH headings : Cyclization; Microwaves; Molecular Structure; Phenanthridines / chemical synthesis; Phenanthridines / chemistry; Phenanthridines / radiation effects; Stereoisomerism
topics (OpenAlex): Chemical synthesis and alkaloids; Cyclopropane Reaction Mechanisms
TL;DR:
A concise synthesis of phenanthridines via a microwave-assisted [2+2+3+2] cyclotrimerization reaction has been developed and is applicable to selective separation of Na6(CO3)(SO4)2, Na2CO3, and Na2SO4 from Na3SO4-based polymers.
(via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018
2008 journal article
Small-molecule inhibitors of microRNA miR-21 function
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 47(39), 7482–7484.
author keywords: antitumor agents; cell-based assays; inhibitors; medicinal chemistry; microRNA
MeSH headings : Gene Expression; Genes, Reporter / genetics; HeLa Cells; Humans; MicroRNAs / antagonists & inhibitors; MicroRNAs / genetics; Molecular Structure
topics (OpenAlex): MicroRNA in disease regulation; RNA Interference and Gene Delivery; Advanced biosensing and bioanalysis techniques
TL;DR:
A cellular screen is reported for miRNA pathway inhibitors and the first small molecule modifiers of miRNA function, which are likely to be important tools to elucidate the detailed mechanisms of mi RNA function and provide lead structures for the development of new therapeutics.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2008 journal article
Synthesis and investigation of the 5-formylcytidine modified, anticodon stem and loop of the human mitochondrial tRNA(Met)
NUCLEIC ACIDS RESEARCH, 36(20), 6548–6557.
MeSH headings : Anticodon / chemistry; Base Sequence; Codon / metabolism; Cytidine / analogs & derivatives; Cytidine / chemistry; Humans; Molecular Sequence Data; Nucleic Acid Conformation; Protein Biosynthesis; RNA / chemistry; RNA, Mitochondrial; RNA, Transfer, Met / chemical synthesis; RNA, Transfer, Met / chemistry; RNA, Transfer, Met / metabolism; Thermodynamics
topics (OpenAlex): RNA modifications and cancer; RNA and protein synthesis mechanisms; RNA Research and Splicing
TL;DR:
The first synthesis and analyses of the tRNA's anticodon stem and loop domain containing the 5-formylcytidine modification are reported, contributing to the tRNAs anticodon domain structure, thermodynamic properties and its ability to bind codons AUA and AUG in translational initiation and elongation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2008 journal article
Synthesis of Anthracene and Azaanthracene Fluorophores via [2+2+2] Cyclotrimerization Reactions
ORGANIC LETTERS, 10(20), 4661–4664.
MeSH headings : Anthracenes / chemical synthesis; Anthracenes / chemistry; Aza Compounds / chemical synthesis; Aza Compounds / chemistry; Benzene / chemistry; Cell Line; Cyclization; Fluorescent Dyes / chemical synthesis; Fluorescent Dyes / chemistry; Humans; Molecular Structure; Pyridines / chemistry
topics (OpenAlex): Chemical Synthesis and Analysis; Click Chemistry and Applications; Catalytic Cross-Coupling Reactions
TL;DR:
A highly convergent [2-2+2] cyclotrimerization approach to anthracenes and 2-azaanthracenes has been developed that allows for the facile introduction of the anthracene moiety on alkyne and nitrile bearing molecules and the rapid construction of compound arrays.
(via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018
2007 journal article
A general approach to chemo- and regioselective cyclotrimerization reactions
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 46(27), 5187–5190.
author keywords: cycloaddition; heterocycles; microwave synthesis; solid-phase synthesis; transition metals
topics (OpenAlex): Catalytic Alkyne Reactions; Synthesis and Biological Evaluation
Source: Web Of Science
Added: August 6, 2018
2007 journal article
Microwave-assisted solid-supported alkyne cyclotrimerization reactions for combinatorial chemistry
JOURNAL OF COMBINATORIAL CHEMISTRY, 9(5), 735–738.
MeSH headings : Alkynes / chemistry; Combinatorial Chemistry Techniques; Cyclization; Microwaves
topics (OpenAlex): Catalytic Alkyne Reactions; Multicomponent Synthesis of Heterocycles; Microwave-Assisted Synthesis and Applications
Source: Web Of Science
Added: August 6, 2018
2007 journal article
Photochemical DNA activation
ORGANIC LETTERS, 9(10), 1903–1906.
MeSH headings : DNA, Catalytic / chemistry; DNA, Catalytic / metabolism; Molecular Sequence Data; Molecular Structure; Photochemistry; RNA / metabolism; Substrate Specificity
topics (OpenAlex): Advanced biosensing and bioanalysis techniques; DNA and Nucleic Acid Chemistry; Photochromic and Fluorescence Chemistry
TL;DR:
A new photocaged nucleoside was synthesized and incorporated into DNA with the use of standard synthesis conditions and Brief irradiation with nonphotodamaging UV light led to rapid decaging and almost quantitative restoration of DNAzyme activity.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2007 journal article
Photochemical activation of protein expression in bacterial cells
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 46(23), 4290–4292.
author keywords: bacterial cells; caged compounds; gene expression; photochemistry; proteins
topics (OpenAlex): Photochromic and Fluorescence Chemistry; bioluminescence and chemiluminescence research; Photoreceptor and optogenetics research
TL;DR:
A light-inducible gene-expression system which can be used in bacterial cells, plants, and mammalian organisms is reported, based on the lactose (lac) repressor which binds to the lac operator ( lacO), thereby inhibiting RNA polymerase from performing gene transcription.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2007 journal article
Photochemical control of biological processes
ORGANIC & BIOMOLECULAR CHEMISTRY, 5(7), 999–1005.
MeSH headings : Enzymes / genetics; Enzymes / radiation effects; Humans; Light; Molecular Conformation; Organic Chemicals / chemistry; Organic Chemicals / radiation effects; Photochemistry; Proteins / genetics; Proteins / radiation effects; Ultraviolet Rays
topics (OpenAlex): Photochromic and Fluorescence Chemistry; bioluminescence and chemiluminescence research; Click Chemistry and Applications
TL;DR:
This report summarizes the advances made in recent years, focusing predominantly on the in vivo regulation of gene function using irradiation with UV light, using photocaging groups installed either on a small molecule modulator of biomolecular function or directly on a biological macromolecule itself.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2007 journal article
Synthesis of indanones via solid-supported [2+2+2] cyclotrimerization
JOURNAL OF ORGANIC CHEMISTRY, 72(20), 7801–7804.
topics (OpenAlex): Synthesis and biological activity; Synthesis of Indole Derivatives; Synthesis of Organic Compounds
TL;DR:
A new facile approach toward natural and unnatural indanones has been developed, featuring a solid-supported [2+2+3+2] cyclotrimerization as the key step.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2006 journal article
A genetically encoded fluorescent amino acid
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 103(26), 9785–9789.
author keywords: molecular evolution; fluorescent probes; genetic code expansion; protein design; unnatural amino acids
MeSH headings : Alanine / analogs & derivatives; Alanine / chemistry; Alanine / genetics; Alanine / metabolism; Amino Acids / chemistry; Amino Acids / genetics; Amino Acids / metabolism; Amino Acyl-tRNA Synthetases / genetics; Amino Acyl-tRNA Synthetases / metabolism; Codon, Nonsense / metabolism; Dansyl Compounds / chemistry; Dansyl Compounds / metabolism; Fluorescent Dyes / chemistry; Genetic Code; Humans; RNA, Transfer / genetics; RNA, Transfer / metabolism; Saccharomyces cerevisiae / genetics; Superoxide Dismutase / chemistry; Superoxide Dismutase / metabolism
topics (OpenAlex): RNA and protein synthesis mechanisms; Fungal and yeast genetics research; Bacterial Genetics and Biotechnology
TL;DR:
The strategy described here should be applicable to a number of different fluorophores in both prokaryotic and eukaryotic organisms, and it should facilitate both biochemical and cellular studies of protein structure and function.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2006 journal article
A genetically encoded photocaged tyrosine
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 45(17), 2728–2731.
author keywords: amino acids; mutagenesis; photocaged tyrosine; proteins
MeSH headings : Molecular Structure; Photochemistry; Tyrosine / chemistry; Tyrosine / genetics; beta-Galactosidase / metabolism
topics (OpenAlex): RNA and protein synthesis mechanisms; CRISPR and Genetic Engineering; Bacterial Genetics and Biotechnology
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2006 journal article
A new photocaging group for aromatic N-heterocycles
SYNTHESIS-STUTTGART, (13), 2147–2150.
author keywords: bioorganic chemistry; heterocycles; photocaging; protecting groups
topics (OpenAlex): Photochromic and Fluorescence Chemistry; Radical Photochemical Reactions; bioluminescence and chemiluminescence research
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2006 journal article
Conditional transgene and gene targeting methodologies in zebrafish
Zebrafish, 3, 415–429.
Source: NC State University Libraries
Added: August 6, 2018
2006 review
General strategy for the syntheses of corynanthe, tacaman, and oxindole alkaloids
[Review of ]. JOURNAL OF ORGANIC CHEMISTRY, 71(17), 6547–6561.
MeSH headings : Alkaloids / chemical synthesis; Alkaloids / chemistry; Indole Alkaloids / chemical synthesis; Indole Alkaloids / chemistry; Indoles / chemical synthesis; Indoles / chemistry; Lactams / chemistry; Models, Molecular; Molecular Structure; Oxidation-Reduction; Oxindoles; Pausinystalia; Piperidines / chemistry
topics (OpenAlex): Synthetic Organic Chemistry Methods; Chemical synthesis and alkaloids; Asymmetric Synthesis and Catalysis
TL;DR:
The total synthesis of the c Corynanthe alkaloid dihydrocorynantheol and the formal syntheses of the indole alkaloids tacamonine, rhynchophylline, and hirsutine are reported.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2006 journal article
Photochemical hammerhead ribozyme activation
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 16(10), 2658–2661.
author keywords: caged compounds; genomics; photochemistry; ribozymes; RNA
MeSH headings : Base Sequence; Electrophoresis, Polyacrylamide Gel; Enzyme Activation; Nucleic Acid Conformation; Photochemistry; RNA, Catalytic / chemistry; RNA, Catalytic / metabolism; Ultraviolet Rays
topics (OpenAlex): Advanced biosensing and bioanalysis techniques; RNA and protein synthesis mechanisms; Molecular Junctions and Nanostructures
TL;DR:
The light-activation of allosteric cis and trans acting ribozymes via decaging of a small organic molecule ligand is reported through an optimized N-caging group based on a nitrobenzyl core structure that can potentially be employed toward a light-induced control of gene function.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2006 journal article
Pyridines via solid-supported [2+2+2] cyclotrimerization
Chemical Communications (Cambridge, England), (12), 1313–1315.
topics (OpenAlex): Chemical Synthesis and Analysis; Cyclopropane Reaction Mechanisms; Catalytic Alkyne Reactions
TL;DR:
The formation of pyridines via a crossed [2 + 2 + 2] cycloaddition has been achieved on a solid-support for the first time.
(via Semantic Scholar)
Source: NC State University Libraries
Added: August 6, 2018
2006 journal article
Solid-supported [2+2+2] cyclotrimerizations
CHEMISTRY-A EUROPEAN JOURNAL, 12(21), 5563–5568.
author keywords: combinatorial chemistry; cyclotrimerization; homogeneous catalysis; solid-phase synthesis
topics (OpenAlex): Synthetic Organic Chemistry Methods; Catalytic Alkyne Reactions; Microbial Natural Products and Biosynthesis
TL;DR:
The transition-metal-catalyzed [2+2-2] cyclotrimerization of a diyne and an alkyne provides a convergent route to highly-substituted aromatic rings and enables rapid combinatorial access to diverse sets of carbo- and heterocyclic small-molecule arrays.
(via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018
2004 review
Synthesis of oxygen- and nitrogen-containing heterocycles by ring-closing metathesis
[Review of ]. CHEMICAL REVIEWS, 104(5), 2199–2238.
MeSH headings : Heterocyclic Compounds / chemical synthesis; Heterocyclic Compounds / chemistry; Molecular Structure; Nitrogen / chemistry; Oxygen / chemistry
topics (OpenAlex): Synthetic Organic Chemistry Methods; Asymmetric Synthesis and Catalysis; Chemical Synthesis and Analysis
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018