@article{ahmad_din_khan_rehman_hassan_aziz_alharbi_wu_2024, title={<i>Lacticaseibacillus</i><i>paracasei</i> BNCC345679 revolutionizes DSS-induced colitis and modulates gut microbiota}, volume={15}, ISSN={["1664-302X"]}, DOI={10.3389/fmicb.2024.1343891}, abstractNote={The gut microbiota plays an important role in the disease progression of inflammatory bowel disease. Although probiotics are effective against IBD, not many studies have investigated their effects on gut microbiota composition and immunomodulation in mouse colitis models. Our study aimed at the therapeutic effects of Lacticaseibacillus paracasei BNCC345679 for the first time and explored its impact on gut microbiome dysbiosis, inflammatory cytokines, related miRNAs, VCAM-1, oxidative stress, intestinal integrity, and mucus barrier. We found that oral intervention of L. paracasei BNCC345679 affects recovering beneficial microbial taxa, including lactobacillus spp. and akkermansia spp., followed by improved body weight, DAI score, and inflammatory cytokines. L. paracasei BNCC345679 mitigated oxidative stress and increased the expression of intestinal integrity proteins MUC2 and ZO-1. These results suggested that L. paracasei BNCC345679 has the capacity to reduce DSS-induced colitis and has the potential as a supplement for the mitigation of IBD.}, journal={FRONTIERS IN MICROBIOLOGY}, author={Ahmad, Waqar and Din, Ahmad Ud and Khan, Taj Malook and Rehman, Mujeeb Ur and Hassan, Adil and Aziz, Tariq and Alharbi, Metab and Wu, Jianbo}, year={2024}, month={Mar} }
 @article{junaid_lu_din_yu_liu_li_liu_yan_qi_2024, title={Deciphering Microbiome, Transcriptome, and Metabolic Interactions in the Presence of Probiotic Lactobacillus acidophilus against Salmonella Typhimurium in a Murine Model}, url={https://doi.org/10.3390/antibiotics13040352}, DOI={10.3390/antibiotics13040352}, abstractNote={Salmonella enterica serovar Typhimurium (S. Typhimurium), a foodborne pathogen that poses significant public health risks to humans and animals, presents a formidable challenge due to its antibiotic resistance. This study explores the potential of Lactobacillus acidophilus (L. acidophilus 1.3251) probiotics as an alternative strategy to combat antibiotic resistance associated with S. Typhimurium infection. In this investigation, twenty-four BALB/c mice were assigned to four groups: a non-infected, non-treated group (CNG); an infected, non-treated group (CPG); a group fed with L. acidophilus but not infected (LAG); and a group fed with L. acidophilus and challenged with Salmonella (LAST). The results revealed a reduction in Salmonella levels in the feces of mice, along with restored weight and improved overall health in the LAST compared to the CPG. The feeding of L. acidophilus was found to downregulate pro-inflammatory cytokine mRNA induced by Salmonella while upregulating anti-inflammatory cytokines. Additionally, it influenced the expression of mRNA transcript, encoding tight junction protein, oxidative stress-induced enzymes, and apoptosis-related mRNA expression. Furthermore, the LEfSe analysis demonstrated a significant shift in the abundance of critical commensal genera in the LAST, essential for maintaining gut homeostasis, metabolic reactions, anti-inflammatory responses, and butyrate production. Transcriptomic analysis revealed 2173 upregulated and 506 downregulated differentially expressed genes (DEGs) in the LAST vs. the CPG. Functional analysis of these DEGs highlighted their involvement in immunity, metabolism, and cellular development. Kyoto Encyclopedia of Genes and Genome (KEGG) pathway analysis indicated their role in tumor necrosis factor (TNF), mitogen-activated protein kinase (MAPK), chemokine, Forkhead box O (FOXO), and transforming growth factor (TGF-β) signaling pathway. Moreover, the fecal metabolomic analysis identified 929 differential metabolites, with enrichment observed in valine, leucine, isoleucine, taurine, glycine, and other metabolites. These findings suggest that supplementation with L. acidophilus promotes the growth of beneficial commensal genera while mitigating Salmonella-induced intestinal disruption by modulating immunity, gut homeostasis, gut barrier integrity, and metabolism.}, journal={Antibiotics}, author={Junaid, Muhammad and Lu, Hongyu and Din, Ahmad Ud and Yu, Bin and Liu, Yu and Li, Yixiang and Liu, Kefei and Yan, Jianhua and Qi, Zhongquan}, year={2024}, month={Apr} }
 @misc{luqman_he_hassan_ullah_zhang_rashid khan_din_ullah_wang_wang_2024, title={Mood and microbes: a comprehensive review of intestinal microbiota's impact on depression}, volume={15}, ISSN={["1664-0640"]}, DOI={10.3389/fpsyt.2024.1295766}, abstractNote={Depression is considered a multifaceted and intricate mental disorder of growing concern due to its significant impact on global health issues. The human gut microbiota, also known as the “second brain,” has an important role in the CNS by regulating it through chemical, immunological, hormonal, and neurological processes. Various studies have found a significant bidirectional link between the brain and the gut, emphasizing the onset of depression therapies. The biological and molecular processes underlying depression and microbiota are required, as the bidirectional association may represent a novel study. However, profound insights into the stratification and diversity of the gut microbiota are still uncommon. This article investigates the emerging evidence of a bacterial relationship between the gut and the brain’s neurological system and its potential pathogenicity and relevance. The interplay of microbiota, immune system, nervous system neurotransmitter synthesis, and neuroplasticity transitions is also widely studied. The consequences of stress, dietary fibers, probiotics, prebiotics, and antibiotics on the GB axis are being studied. Multiple studies revealed the processes underlying this axis and led to the development of effective microbiota-based drugs for both prevention and treatment. Therefore, the results support the hypothesis that gut microbiota influences depression and provide a promising area of research for an improved knowledge of the etiology of the disease and future therapies.}, journal={FRONTIERS IN PSYCHIATRY}, author={Luqman, Ameer and He, Mei and Hassan, Adil and Ullah, Mehtab and Zhang, Liyuan and Rashid Khan, Muhammad and Din, Ahmad Ud and Ullah, Kamran and Wang, Wei and Wang, Guixue}, year={2024}, month={Feb} }
 @article{junaid_lu_li_liu_din_qi_xiong_yan_2024, title={Novel Synergistic Probiotic Intervention: Transcriptomic and Metabolomic Analysis Reveals Ameliorative Effects on Immunity, Gut Barrier, and Metabolism of Mice during Salmonella typhimurium Infection}, url={https://doi.org/10.3390/genes15040435}, DOI={10.3390/genes15040435}, abstractNote={Salmonella typhimurium (S. typhimurium), a prevalent cause of foodborne infection, induces significant changes in the host transcriptome and metabolome. The lack of therapeutics with minimal or no side effects prompts the scientific community to explore alternative therapies. This study investigates the therapeutic potential of a probiotic mixture comprising Lactobacillus acidophilus (L. acidophilus 1.3251) and Lactobacillus plantarum (L. plantarum 9513) against S. typhimurium, utilizing transcriptome and metabolomic analyses, a novel approach that has not been previously documented. Twenty-four SPF-BALB/c mice were divided into four groups: control negative group (CNG); positive control group (CPG); probiotic-supplemented non-challenged group (LAPG); and probiotic-supplemented Salmonella-challenged group (LAPST). An RNA-sequencing analysis of small intestinal (ileum) tissue revealed 2907 upregulated and 394 downregulated DEGs in the LAPST vs. CPG group. A functional analysis of DEGs highlighted their significantly altered gene ontology (GO) terms related to metabolism, gut integrity, cellular development, and immunity (p ≤ 0.05). The KEGG analysis showed that differentially expressed genes (DEGs) in the LAPST group were primarily involved in pathways related to gut integrity, immunity, and metabolism, such as MAPK, PI3K-Akt, AMPK, the tryptophan metabolism, the glycine, serine, and threonine metabolism, ECM–receptor interaction, and others. Additionally, the fecal metabolic analysis identified 1215 upregulated and 305 downregulated metabolites in the LAPST vs. CPG group, implying their involvement in KEGG pathways including bile secretion, propanoate metabolism, arginine and proline metabolism, amino acid biosynthesis, and protein digestion and absorption, which are vital for maintaining barrier integrity, immunity, and metabolism. In conclusion, these findings suggest that the administration of a probiotic mixture improves immunity, maintains gut homeostasis and barrier integrity, and enhances metabolism in Salmonella infection.}, journal={Genes}, author={Junaid, Muhammad and Lu, Hongyu and Li, Yixiang and Liu, Yu and Din, Ahmad Ud and Qi, Zhongquan and Xiong, Yi and Yan, Jianhua}, year={2024}, month={Mar} }
 @misc{luqman_hassan_ullah_naseem_ullah_zhang_din_ullah_ahmad_wang_2024, title={Role of the intestinal microbiome and its therapeutic intervention in cardiovascular disorder}, volume={15}, ISSN={["1664-3224"]}, DOI={10.3389/fimmu.2024.1321395}, abstractNote={The gut microbiome is a heterogeneous population of microbes comprising viruses, bacteria, fungi, and protozoa. Such a microbiome is essential for sustaining host equilibrium, and its impact on human health can be altered by a variety of factors such as external variables, social behavior, age, nutrition, and genetics. Gut microbes’ imbalances are related to a variety of chronic diseases including cancer, obesity, and digestive disorders. Globally, recent findings show that intestinal microbes have a significant role in the formation of cardiovascular disease (CVD), which is still the primary cause of fatalities. Atherosclerosis, hypertension, diabetes, inflammation, and some inherited variables are all cardiovascular risk variables. However, studies found correlations between metabolism, intestinal flora, and dietary intake. Variations in the diversity of gut microbes and changes in their activity are thought to influence CVD etiology. Furthermore, the gut microbiota acts as an endocrine organ, producing bioactive metabolites such as TMA (trimethylamine)/TMAO (trimethylamine N-oxide), SCFA (short-chain fatty acids), and bile acids, which have a substantial impact on host wellness and disease by multiple mechanisms. The purpose of this overview is to compile current evidence highlighting the intricate links between gut microbiota, metabolites, and the development of CVD. It focuses on how intestinal dysbiosis promotes CVD risk factors such as heart failure, hypertension, and atherosclerosis. This review explores the normal physiology of intestinal microbes and potential techniques for targeting gut bacteria for CVD treatment using various microbial metabolites. It also examines the significance of gut bacteria in disease treatment, including supplements, prebiotics, probiotics, antibiotic therapies, and fecal transplantation, which is an innovative approach to the management of CVD. As a result, gut bacteria and metabolic pathways become increasingly attractive as potential targets for CVD intervention.}, journal={FRONTIERS IN IMMUNOLOGY}, author={Luqman, Ameer and Hassan, Adil and Ullah, Mehtab and Naseem, Sahar and Ullah, Mehraj and Zhang, Liyuan and Din, Ahmad Ud and Ullah, Kamran and Ahmad, Waqar and Wang, Guixue}, year={2024}, month={Jan} }
 @article{zhang_qin_qiu_sun_qu_din_yan_li_chen_gu_et al._2023, title={<i>Desulfovibrio desulfuricans</i> aggravates atherosclerosis by enhancing intestinal permeability and endothelial TLR4/NF-KB pathway in <i>Apoe-/-</i> mice}, volume={10}, url={https://publons.com/wos-op/publon/50086270/}, DOI={10.1016/J.GENDIS.2021.09.007}, abstractNote={It is increasingly aware that gut microbiota is closely associated with atherosclerosis. However, which and how specific gut bacteria regulate the progression of atherosclerosis is still poorly understood. In this study, modified linear discriminant analysis was performed in comparing the gut microbiota structures of atherosclerotic and non-atherosclerotic mice, and Desulfovibrio desulfuricans (D. desulfuricans) was found to be associated with atherosclerosis. D. desulfuricans-treated Apoe−/− mice showed significantly aggravated atherosclerosis. The proatherogenic effect of D. desulfuricans was attributed to its ability to increase intestinal permeability and subsequent raise in the transit of lipopolysaccharide (LPS) from the intestine to the bloodstream. Excessive LPS in the blood can elicit local and systemic inflammation and activate Toll-like receptor 4 (TLR4)/nuclear factor-κB (NF-κB) signaling of endothelial cells. TAK-242, a specific inhibitor of TLR4, can ameliorate the development of D. desulfuricans-induced atherosclerosis by blocking the LPS-induced activation of TLR4/NF-κB signaling.}, journal={Genes & Diseases}, publisher={Elsevier BV}, author={Zhang, Kun and Qin, Xian and Qiu, Juhui and Sun, Tong and Qu, Kai and Din, Ahmad Ud and Yan, Wenhua and Li, Tianhan and Chen, Yidan and Gu, Wei and et al.}, year={2023} }
 @article{ullah_din_ding_shi_pervaz_shen_2023, title={A narrative review: CXC chemokines influence immune surveillance in obesity and obesity-related diseases: Type 2 diabetes and nonalcoholic fatty liver disease}, url={https://doi.org/10.1007/s11154-023-09800-w}, DOI={10.1007/s11154-023-09800-w}, abstractNote={Adipose tissue develops lipids, aberrant adipokines, chemokines, and pro-inflammatory cytokines as a consequence of the low-grade systemic inflammation that characterizes obesity. This low-grade systemic inflammation can lead to insulin resistance (IR) and metabolic complications, such as type 2 diabetes (T2D) and nonalcoholic fatty liver disease (NAFLD). Although the CXC chemokines consists of numerous regulators of inflammation, cellular function, and cellular migration, it is still unknown that how CXC chemokines and chemokine receptors contribute to the development of metabolic diseases (such as T2D and NAFLD) during obesity. In light of recent research, the objective of this review is to provide an update on the linkage between the CXC chemokine, obesity, and obesity-related metabolic diseases (T2D and NAFLD). We explore the differential migratory and immunomodulatory potential of CXC chemokines and their mechanisms of action to better understand their role in clinical and laboratory contexts. Besides that, because CXC chemokine profiling is strongly linked to leukocyte recruitment, macrophage recruitment, and immunomodulatory potential, we hypothesize that it could be used to predict the therapeutic potential for obesity and obesity-related diseases (T2D and NAFLD).}, journal={Reviews in Endocrine and Metabolic Disorders}, author={Ullah, Amin and Din, Ahmad Ud and Ding, Wen and Shi, Zheng and Pervaz, Sadaf and Shen, Bairong}, year={2023}, month={Aug} }
 @article{xue_ding_qin_liu_ding_zhou_liu_singla_shen_din_et al._2023, title={Assessment of the impact of intravenous antibiotics treatment on gut microbiota in patients: Clinical data from pre-and post-cardiac surgery}, volume={12}, url={http://dx.doi.org/10.3389/fcimb.2022.1043971}, DOI={10.3389/fcimb.2022.1043971}, abstractNote={Background and aimsSurgical site infection is a common complication after surgery. Periprocedural antibiotics are necessary to prescribe for preventing or treating infections. The present study aimed to explore the effect of intravenous antibiotics on gut microbiota and menaquinone biosynthesis in patients, especially in elderly patients undergoing cardiac surgery.}, journal={Frontiers in Cellular and Infection Microbiology}, publisher={Frontiers Media SA}, author={Xue, Ling and Ding, Yinglong and Qin, Qiong and Liu, Linsheng and Ding, Xiaoliang and Zhou, Yi and Liu, Kun and Singla, Rajeev K. and Shen, Ke and Din, Ahmad Ud and et al.}, year={2023}, month={Jan} }
 @article{xu_tang_din_lu_ma_zhang_wu_zuoqin_luo_wu_2023, title={Oligosaccharides of Polygonatum Cyrtonema Hua ameliorates dextran sulfate sodium-induced colitis and regulates the gut microbiota}, volume={161}, url={http://dx.doi.org/10.1016/j.biopha.2023.114562}, DOI={10.1016/j.biopha.2023.114562}, abstractNote={Ulcerative colitis (UC) is one common chronic inflammatory bowel disease that causes severe side effects, and expensive treatment limits effective and sustained treatment of UC. Fructooligosaccharide was isolated from Polygonatum Cyrtonema Hua (PFOS) and exhibits anti-inflammatory effects. Therefore, we are curious whether PFOS could be used for the treatment of UC. PFOS was introduced via intragastric gavage to C57BL/6 J mice exposed to acute colitis induced by DSS. The results showed that doses of PFOS at 2 and 5 mg/kg/day alleviated the DSS-induced histopathological damage and improved intestinal barrier function. qPCR analysis revealed that PFOS exerted a significant downregulation of pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6) and upregulation of antioxidant genes, including superoxide dismutase1 (SOD1), glutathion peroxidase2 (GPX2), and nuclear factor erythroid 2 related factor2 (Nrf2). Furthermore, PFOS suppressed the DSS-induced disruption of the mucosal barrier by downregulating MMP13. Moreover, using 16 S rRNA gene-based microbiota analysis, PFOS could selectively enhance the growth of probiotics, including Bifidobacterium, Alloprevofella, and Alistipes. Our findings indicated that PFOS attenuated DSS-induced colitis in mice, suggesting that PFOS might be used as an efficacious supplement for reducing inflammatory bowel disease.}, journal={Biomedicine & Pharmacotherapy}, publisher={Elsevier BV}, author={Xu, Jin and Tang, Chuankang and Din, Ahmad Ud and Lu, Yu and Ma, Xiaoyu and Zhang, Tao and Wu, Jiaqi and Zuoqin, Du and Luo, Pei and Wu, Jianbo}, year={2023}, month={May}, pages={114562} }
 @article{shen_din_sinha_zhou_qian_shen_2023, title={Translational informatics for human microbiota: data resources, models and applications}, volume={24}, url={http://dx.doi.org/10.1093/bib/bbad168}, DOI={10.1093/bib/bbad168}, abstractNote={Abstract}, number={3}, journal={Briefings in Bioinformatics}, publisher={Oxford University Press (OUP)}, author={Shen, Ke and Din, Ahmad Ud and Sinha, Baivab and Zhou, Yi and Qian, Fuliang and Shen, Bairong}, year={2023}, month={May} }
 @article{emerging piezo1 signaling in inflammation and atherosclerosis; a potential therapeutic target_2022, url={https://publons.com/publon/51218649/}, DOI={10.7150/IJBS.63819}, abstractNote={Purpose of Review: Atherosclerosis is the principal cause of cardiovascular diseases (CVDs) which are the major cause of death worldwide. Mechanical force plays an essential role in cardiovascular health and disease. To bring the awareness of mechanosensitive Piezo1 role in atherosclerosis and its therapeutic potentials we review recent literature to highlight its involvement in various mechanisms of the disease. Recent Findings: Recent studies reported Piezo1 channel as a sensor, and transducer of various mechanical forces into biochemical signals, which affect various cellular activities such as proliferation, migration, apoptosis and vascular remodeling including immune/inflammatory mechanisms fundamental phenomenon in atherogenesis. Summary: Numerous evidences suggest Piezo1 as a player in different mechanisms of cell biology, including immune/inflammatory and other cellular mechanisms correlated with atherosclerosis. This review discusses mechanistic insight about this matter and highlights the drugability and therapeutic potentials consistent with emerging functions Piezo1 in various mechanisms of atherosclerosis. Based on the recent works, we suggest Piezo1 as potential therapeutic target and a valid candidate for future research. Therefore, a deeper exploration of Piezo1 biology and translation towards the clinic will be a novel strategy for treating atherosclerosis and other CVDs.}, journal={International Journal of Biological Sciences}, year={2022} }
 @article{ullah_khan_basharat_huo_din_wang_2022, title={Evaluation of Cardiac Biomarkers and Expression Analysis of IL-1, IL-6, IL-10, IL-17, and IL-25 among COVID-19 Patients from Pakistan}, url={https://doi.org/10.3390/v14102149}, DOI={10.3390/v14102149}, abstractNote={Coronavirus disease 19 (COVID-19) is caused by viral infection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Where upregulation of several important biomarkers and multiple organ dysfunction occurs, this study aimed to evaluate the association of cardiac biomarkers and CS induced acute lung damage with disease severity and mortality in survival of COVID-19 patients. A total of 500 COVID-19 patients with elevated cardiac biomarkers were studied for the analysis of myocardial abnormality through cardiac enzymes, inflammatory biomarkers, and the expression analysis of various cytokines, including IL-1, IL-6, IL-10, IL-17, and IL-25 genes. The elevation of various cardiac enzymes including LDH (87%), CK (78.4%), TNI (80.4%), CK-MB (83%), and D-dimer (80.8%) were found correlated (p < 0.001) with COVID-19 infection. Cardiac enzyme elevation was highly associated with an increased level of inflammatory biomarkers such as CRP (14.2%), SAA (11.4%) and erythrocyte sedimentation rate (ESR) (7.8%) (p = 0.001 for all). The quantitative expression analysis of IL-10, 1L-17, and 1L-25 were found to be high, while those of IL-1 and IL-6 were moderately elevated. The death-to-live ratio of COVID-19 patients was 457:43 indicating that the patients having elevated levels of both CKMB, D-dimer, CK and IL-1, IL-6, IL-10 and D-dimer, Troponin, CK and IL-1, IL-10 had high fatality rate (73% and 12% respectively). The current finding concludes that the evaluation of cardiac biomarkers with cytokine storm plays a significant role in COVID-19-associated anatomical organ damage, myocardial injury, and mortality. Physicians should pay special attention to cardiac biomarkers in patients with old age, inflammation, and comorbidities among COVID-19 infections.}, journal={Viruses}, author={Ullah, Razi and Khan, Jadoon and Basharat, Nosheen and Huo, Danqun and Din, Ahmad Ud and Wang, Guixue}, year={2022}, month={Sep} }
 @article{zafar_alam_sabir_saba_din_ahmad_khan_muhammad_dayisoylu_2022, title={Isolation, characterization, bacteriocin production and biological potential of Bifidobacteria of ruminants}, volume={658}, url={http://dx.doi.org/10.1016/j.ab.2022.114926}, DOI={10.1016/j.ab.2022.114926}, abstractNote={Bacteriocins exhibited a wide spectrum of antibacterial activity against different pathogens. The aim of current study was to characterize the bacteriocins produced by Bifidobacterium spp. isolated from ruminants. The Bifidobacterium isolates were identified as B. longum, B. pseudolongum, B. bifidum, B. thermophilum, B. boum, B. merycicum and B. ruminantium. Bacteriocins were found to be pH stable, heat resistant, highly diffusible, NaCl tolerant and resistant to UV radiations. SDS, EDTA and urea induced 14%, 21% and 24% bacteriocins activity loss. Modified MRS broth (1% tryptone, 1% yeast extract and 2% glucose) was found to be the best nutrient medium for optimal production of bacteriocins. Minimum inhibitory concentration (MIC) values varied from 300 μl/ml to 500 μl/ml and minimum bactericidal concentration (MBC) values ranged from 500 μl/ml to >500 μl/ml for E. coli and S. aureus respectively. The highest protein concentration (29.0248 mg/ml) was recorded for Bifidobacteria bacteriocin produced by B. longum. Tricine-Sodium Dodecyl Sulfate-Poly Acrylamide Gel Electrophoresis (SDS-PAGE) revealed that molecular weight of isolated bifidobacterial bacteriocins was in the range of 3.6 kDa-30 kDa. Current study indicated that bifidobacterial bacteriocins have considerable potential to be used as biopreservative.}, journal={Analytical Biochemistry}, publisher={Elsevier BV}, author={Zafar, Mahpara and Alam, Sadia and Sabir, Maimoona and Saba, Nusrat and Din, Ahmad Ud and Ahmad, Rafiq and Khan, Muhammad Rafiullah and Muhammad, Ali and Dayisoylu, Kenan Sinan}, year={2022}, month={Dec}, pages={114926} }
 @article{din_ahmad_khan_wang_wu_2022, title={Metagenomic Analysis of Liquor Starter Culture Revealed Beneficial Microbes’ Presence}, url={https://www.mdpi.com/2304-8158/12/1/25}, DOI={10.3390/foods12010025}, abstractNote={Wines are complex matrices famous for their pleasant aroma and exceptional flavor. Baijiu (white wine) is a traditional Chinese liquor with a soft mouthfeel, fragrant taste, and long-lasting aftertaste. Baijiu is distilled from sorghum and wheat via solid fermentation. As in wines, the microbial ecosystem of Baijiu is a key decisive factor influencing aroma and consumer preferences. Microbial diversity in Baijiu has been intensively investigated. It is important to note that probiotics are a mixture of bacteria and yeast primarily intended to improve health. Our study aimed to characterize the microbial ecosystem of Zaopei Baijiu Daqu (ZBD) starter cultures for specific microbes with probiotic properties. The DNA samples of ZBD starters were analyzed using a metagenomic 16S rRNA approach to characterize the bacterial and ITS for fungal diversity. Weissella cibaria was the most dominant species in the bacterial community, while Saccharomycopsis fibuligera was the most abundant fungal species. Furthermore, functional prediction analysis identified unique pathways associated with microbial diversity relevant to functional innovation. These associated pathways include fermentation, amino acid metabolism, carbohydrate metabolism, energy metabolism, and membrane transport. This study identified beneficial microbes in the starter culture, opening a path for further in-depth analysis of those microbes by isolating and evaluating them for a valuable role in in vitro and in vivo studies.}, journal={Foods}, author={Din, Ahmad Ud and Ahmad, Waqar and Khan, Taj Malook and Wang, Jun and Wu, Jianbo}, year={2022}, month={Dec} }
 @article{sadiq_khan_ullah_basharat_ali_din_ali_farid_shah_abdel-daim_et al._2022, title={Seroprevalence of Anti-tTg-IgA among Symptomized Celiac Disease Patients and Their Correlation with Rotavirus Infection}, url={https://doi.org/10.1155/2022/6972624}, DOI={10.1155/2022/6972624}, abstractNote={Celiac disease (CD) is a chronic inflammatory disorder in the intestinal tract as a response to the use of gluten in genetically predisposed individuals. It is a worldwide problem, with a high prevalence rate in North America. This is a descriptive cross-sectional study involving 1090 samples collected from different hospitals of Rawalpindi and Islamabad, Pakistan, from January 2019 to December 2019. In this study, 1090 blood samples screened for seroprevalence of anti-tTG antibodies in CD symptomatic patients via ELISA (enzyme-linked immunosorbent assay). 1090 fecal samples from the same CD patients were collected and tested for the presence of rotavirus (RV) via ELISA and RT-PCR. Of the 1090 patients tested for seroprevalence of anti-tTG antibodies, 112/1090 (10.3%) were found to be positive. Of the 112 anti-tTG-positive patients, 78/112 (70%) were positive for RV via ELISA and 74/112 (66.1%) were RV positive via RT-PCR. A statistically significant association was reported between rotavirus infection and celiac disease (
                     
                        p
                        ˂
                        0.05
                     
                  ). Anti-tTG antibodies were higher in age group 6 (12-18 years) patients (18.4%) and at minimum in age group 3 (1-3 years) patients (4.8%). However, there was a statistically insignificant relationship between group age and CD prevalence (
                     
                        p
                        >
                        0.05
                     
                  ). The highest CD prevalence was noted during winter season (19.6%) and the lowest (3.0%) during fall/autumn. Our study findings demonstrate that Pakistan has a high prevalence of CD compared to other studies. Further studies in the fields of environmental risk factors and treatment with more advanced serological and histopathological studies are needed in the future.}, journal={BioMed Research International}, author={Sadiq, Asma and Khan, Jadoon and Ullah, Irfan and Basharat, Nosheen and Ali, Sajid and Din, Ahmad Ud and Ali, Ijaz and Farid, Arshad and Shah, Muddaser and Abdel-Daim, Mohamed M. and et al.}, editor={Pan, Hai-FengEditor}, year={2022}, month={Sep} }
 @article{analyzing differentially expressed genes and pathways of bex2-deficient mouse lung via rna-seq_2021, url={https://publons.com/wos-op/publon/35773159/}, DOI={10.3906/BIY-2104-4}, abstractNote={Bex2 is well known for its role in the nervous system, and is associated with neurological disorders, but its role in the lung’s physiology is still not reported. To elucidate the functional role of Bex2 in the lung, we generated a Bex2 knock-out (KO) mouse model using the CRISPR-Cas9 technology and performed transcriptomic analysis. A total of 652 genes were identified as differentially expressed between Bex2 -/- and Bex2 +/+ mice, out of which 500 were downregulated, while 152 were upregulated genes. Among these DEGs, Ucp1, Myh6, Coxa7a1, Myl3, Ryr2, RNaset2b, Npy, Enob1, Krt5, Myl2, Hba-a2, and Nrob2 are the most prominent genes. Myl2, was the most downregulated gene, followed by Npy, Hba-a2, Rnaset2b, nr0b2, Klra8, and Ucp1. Tcte3, Eno1b, Zfp990, and Pcdha9 were the most upregulated DEGs. According to gene enrichment analysis, PPAR pathway, cardiac muscle contraction, and cytokine-cytokine receptor interaction were the most enriched pathways. Besides, the nuclear factor-κB signaling pathway and hematopoietic cell linage pathways were also enriched. Chronic obstructive pulmonary disease (COPD) is enriched among KEGG disease pathways. RT-qPCR assays confirmed the RNA-Seq results. This study opens a new window toward the biological functions of Bex2 in different systems.}, journal={Turkish Journal of Biology}, year={2021} }
 @article{co-infections of two carbapenemase-producing <i>enterobacter hormaechei</i> clinical strains isolated from the same diabetes individual in china_2021, url={https://publons.com/wos-op/publon/38872669/}, DOI={10.1099/JMM.0.001316}, abstractNote={
                  Introduction. Since mcr-1 was first reported in China, there have been ten variants of MCR appearing nationwide so far. Multidrug-resistant 
                     
                        Enterobacteriaceae
                     
                   bacteria carrying both NDM and MCR have become a serious threat to global public health.}, journal={Journal of Medical Microbiology}, year={2021} }
 @article{li_zhang_wang_liang_chen_din_li_zhou_2021, title={Complete genome sequence and probiotic properties of <italic>Lactococcus petauri</italic> LZys1 isolated from healthy human gut}, volume={70}, url={https://publons.com/wos-op/publon/50086277/}, DOI={10.1099/JMM.0.001397}, abstractNote={
                  Introduction. 
                  
                     
                        Lactococcus petauri
                     
                   LZys1 (
                     
                        L. petauri
                     
                   LZys1) is a type of lactic acid bacteria (LAB), which was initially isolated from healthy human gut.}, number={8}, journal={Journal of Medical Microbiology}, publisher={Microbiology Society}, author={Li, Ouyang and Zhang, Huijian and Wang, Wenjing and Liang, Yuxin and Chen, Wenbi and Din, Ahmad Ud and Li, Li and Zhou, Yingshun}, year={2021} }
 @article{arshad_hassan_rehman_huws_cheng_din_2021, title={Gut microbiome colonization and development in neonatal ruminants: Strategies, prospects, and opportunities}, url={https://doi.org/10.1016/j.aninu.2021.03.004}, DOI={10.1016/j.aninu.2021.03.004}, abstractNote={Colonization and development of the gut microbiome is a crucial consideration for optimizing the health and performance of livestock animals. This is mainly attributed to the fact that dietary and management practices greatly influence the gut microbiota, subsequently leading to changes in nutrient utilization and immune response. A favorable microbiome can be implanted through dietary or management interventions of livestock animals, especially during early life. In this review, we explore all the possible factors (for example gestation, colostrum, and milk feeding, drinking water, starter feed, inoculation from healthy animals, prebiotics/probiotics, weaning time, essential oil and transgenesis), which can influence rumen microbiome colonization and development. We discuss the advantages and disadvantages of potential strategies used to manipulate gut development and microbial colonization to improve the production and health of newborn calves at an early age when they are most susceptible to enteric disease. Moreover, we provide insights into possible interventions and their potential effects on rumen development and microbiota establishment. Prospects of latest techniques like transgenesis and host genetics have also been discussed regarding their potential role in modulation of rumen microbiome and subsequent effects on gut development and performance in neonatal ruminants.}, journal={Animal Nutrition}, author={Arshad, Muhammad A. and Hassan, Faiz-ul and Rehman, Muhammad S. and Huws, Sharon A. and Cheng, Yanfen and Din, Ahmad U.}, year={2021}, month={Sep} }
 @article{implication of ferroptosis in aging_2021, url={https://publons.com/wos-op/publon/48395822/}, DOI={10.1038/S41420-021-00553-6}, abstractNote={Abstract}, journal={Cell Death Discovery}, year={2021} }
 @article{ishaq_khan_bacha_shah_hanif_ahmad_ke_li_din_ding_et al._2021, title={Microbiota Targeted Interventions of Probiotic <i>Lactobacillus</i> as an Anti-Ageing Approach: A Review}, url={https://www.mdpi.com/2076-3921/10/12/1930}, DOI={10.3390/antiox10121930}, abstractNote={With the implementation of modern scientific protocols, the average human lifespan has significantly improved, but age-related problems remain a challenge. With the advent of ageing, there are alterations in gut microbiota and gut barrier functions, weak immune responses, increased oxidative stress, and other age-related disorders. This review has highlighted and discussed the current understanding on the significance of gut microbiota dysbiosis and ageing and its inherent effects against age-related oxidative stress as well as on the gut health and gut-brain axis. Further, we have discussed the key mechanism of action of Lactobacillus strains in the longevity of life, alleviating gut dysbiosis, and improving oxidative stress and inflammation to provide an outline of the role of Lactobacillus strains in restoration of gut microbiota dysbiosis and alleviating certain conditions during ageing. Microbiota-targeted interventions of some characterized strains of probiotic Lactobacillus for the restoration of gut microbial community are considered as a potential approach to improve several neurological conditions. However, very limited human studies are available on this alarmed issue and recommend further studies to identify the unique Lactobacillus strains with potential anti-ageing properties and to discover its novel core microbiome-association, which will help to increase the therapeutic potential of probiotic Lactobacillus strains to ageing.}, journal={Antioxidants}, author={Ishaq, Muhammad and khan and Bacha, Ali Sher and Shah, Tariq and Hanif, Anum and Ahmad, Anum Ali and Ke, Wencan and Li, Fuhou and Din, Ahmad Ud and Ding, Zitong and et al.}, year={2021}, month={Nov} }
 @article{anti-atherosclerotic effects of lactobacillus plantarum atcc 14917 in apoe−/− mice through modulation of proinflammatory cytokines and oxidative stress_2020, url={http://dx.doi.org/10.1007/s00253-020-10693-x}, DOI={10.1007/s00253-020-10693-x}, journal={Applied Microbiology and Biotechnology}, year={2020}, month={May} }
 @article{application of a faecalibacterium 16s rdna genetic marker for species identification of dog fecal waste_2020, url={http://dx.doi.org/10.1007/s11356-020-09369-w}, DOI={10.1007/s11356-020-09369-w}, journal={Environmental Science and Pollution Research}, year={2020}, month={May} }
 @article{liu_zhang_din_li_wang_yu_xu_li_zhang_yuan_et al._2020, title={Bacterial association and comparison between lung and intestine in rats}, volume={40}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-85084167768&partnerID=MN8TOARS}, DOI={10.1042/BSR20191570}, abstractNote={Abstract}, number={4}, journal={Bioscience reports}, author={Liu, T.-H. and Zhang, C.-Y. and Din, A.U. and Li, N. and Wang, Q. and Yu, J.-Z. and Xu, Z.-Y. and Li, C.-X. and Zhang, X.-M. and Yuan, J.-L. and et al.}, year={2020} }
 @article{xiangjinyan_zeng_li_zhang_din_zhao_zhou_2020, title={High incidence and characteristic of PRRSV and resistant bacterial Co-Infection in pig farms}, volume={149}, url={http://dx.doi.org/10.1016/j.micpath.2020.104536}, DOI={10.1016/j.micpath.2020.104536}, abstractNote={Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and resistant bacterial co-infection is a serious threat to pig farms. This study was aimed to determine the characteristics of the co-infection of PRRSV with resistant bacterial strains in pig farms. The presence of the PRRSV orf5 gene was confirmed by RT-PCR from 395 samples. Bacterial strains were isolated from PRRSV positive samples. Antimicrobial drug susceptibility was determined by the Kirby-Bauer method. Resistant genes were determined by PCR amplification and sequencing. The whole genome of carbapenems resistant E.coli was sequenced and analyse. A total of 75 samples were PRRSV positive, and 45 different orf5 sequences were finally determined. Phylogenetic analysis showed that 45 sequences are clustered into four groups, including JXA1-like, NADC30-like, GD-QY2-like, and CH-1a-like viruses. Twenty-one samples were identified with PRRSV and amoxicillin resistance bacterial co-infection, and 23 were found with amoxicillin resistance (including 15 Escherichia coli, 3 Klebsiella pneumoniae, 2 Haemophilus parasuis, 1 Actinobacillus pleuropneumoniae, 1 Pasteurella multocida, and 1 Proteus mirabilis). All bacterial strains were resistant to the most commonantibiotics and were carriers of a large number of resistance genes. Whole-genome sequencing of E. coli ScEc7 yielded 113 scaffolds of genome DNA, one IncX3 plasmid pScEc7-NDM-5 (46,161 bp) and one IncF plasmid pScEc7-CTX-M (129,978 bp). It carries19 resistance genes, 8 virulence factors, and several mobile genetic elements. The results obtained let us to concluded that: (1) Co-infection is common in pig farms. (2) The orf5 gene continues to undergo its sequences divergence. (3) The bacterial carrying diverse resistance genes were resistant to most of the commonly used antibiotics. (4) Carbapenems resistant isolate has a large number of resistance genes, virulence factors, and MGEs. Therefore, continuous study of the characteristic of PRRSV and resistant bacterial co-infection is necessary for healthy pig aquaculture.}, journal={Microbial Pathogenesis}, publisher={Elsevier BV}, author={XiangjinYan and Zeng, Jing and Li, Xinyao and Zhang, Zhikun and Din, Ahmad Ud and Zhao, Kelei and Zhou, Yingshun}, year={2020}, month={Sep}, pages={104536} }
 @article{din_hassan_zhu_zhang_wang_li_wang_wang_2020, title={Inhibitory effect of Bifidobacterium bifidum ATCC 29521 on colitis and its mechanism}, volume={79}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-85080950011&partnerID=MN8TOARS}, DOI={10.1016/j.jnutbio.2020.108353}, abstractNote={Probiotics are known to be beneficial in preventing different diseases in model animals, including inflammatory bowel disease. However, there are few studies on probiotics related to miRNA regulation and disease status. In this article, the beneficial role and mechanisms of the probiotic strain Bifidobacterium bifidum ATCC 29521 have been studied in ulcerative colitis using dextran sodium sulphate (DSS) model. Male C57JBL/6 mice were randomly divided into three groups (n=7): Normal group, dextran sulphate sodium (DSS) group, and Bifido group gavage with Bifidobacterium bifidum ATCC 29521 (2×108 CFU/day). Our strain restored the DSS-caused damage by regulating the expression of immune markers and tight junction proteins (TJP) in the colon; briefly by up-regulating ROS-scavenging enzymes (SOD1, SOD2, CAT, and GPX2), anti-inflammatory cytokines (IL-10, PPARγ, IL-6), TJP's (ZO-1, MUC-2, Claudin-3, and E Cadherin-1) and downregulating inflammatory genes (TNF-α, IL-1β) in Bifido group mice. Inflammatory markers appeared to be regulated by NF-κB nuclear P65 subunit, and its translocation was inhibited in Bifido group mice colon. In addition, the expression of inflammatory genes and colonic TJP were also associated with the restoration of miRNAs (miR-150, miR-155, miR-223) in B. bifidum ATCC 29521 treated Bifido group. The dysbiosis executed by DSS was restored in the Bifido group, demonstrating that B. bifidum ATCC 29521 possessed a probiotic role in our DSS colitis mouse model. B. bifidum ATCC 29521 exhibited its probiotic role through its anti-inflammatory role by modulating miRNA-associated TJP and NF-κB regulation and by partially restoring dysbiosis.}, journal={Journal of Nutritional Biochemistry}, author={Din, A.U. and Hassan, A. and Zhu, Y. and Zhang, K. and Wang, Y. and Li, T. and Wang, Y. and Wang, G.}, year={2020} }
 @article{din_mazhar_wasim_ahmad_bibi_hassan_ali_gang_qian_ullah_et al._2020, title={SARS-CoV-2 Microbiome Dysbiosis Linked Disorders and Possible Probiotics Role}, volume={11}, url={http://dx.doi.org/10.1016/j.biopha.2020.110947}, DOI={10.1016/j.biopha.2020.110947}, abstractNote={In December 2019, a pneumonia outbreak of unknown etiology was reported which caused panic in Wuhan city of central China, which was later identified as Coronavirus disease (COVID-19) caused by a novel coronavirus, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by the Chinese Centre for Disease Control and Prevention (CDC) and WHO. To date, the SARS-CoV-2 spread has already become a global pandemic with a considerable death toll. The associated symptoms of the COVID-19 infection varied with increased inflammation as an everyday pathological basis. Among various other symptoms such as fever, cough, lethargy, gastrointestinal (GI) symptoms included diarrhea and IBD with colitis, have been reported. Currently, there is no sole cure for COVID-19, and researchers are actively engaged to search out appropriate treatment and develop a vaccine for its prevention. Antiviral for controlling viral load and corticosteroid therapy for reducing inflammation seems to be inadequate to control the fatality rate. Based on the available related literature, which documented GI symptoms with diarrhea, inflammatory bowel diseases (IBD) with colitis, and increased deaths in the intensive care unit (ICU), conclude that dysbiosis occurs during SARS-COV-2 infection as the gut-lung axis cannot be ignored. As probiotics play a therapeutic role for GI, IBD, colitis, and even in viral infection. So, we assume that the inclusion of studies to investigate gut microbiome and subsequent therapies such as probiotics might help decrease the inflammatory response of viral pathogenesis and respiratory symptoms by strengthening the host immune system, amelioration of gut microbiome, and improvement of gut barrier function.}, journal={Biomedicine & Pharmacotherapy}, publisher={Elsevier BV}, author={Din, Ahmad Ud and Mazhar, Maryam and Wasim, Muhammad and Ahmad, Waqar and Bibi, Asma and Hassan, Adil and Ali, Niaz and Gang, Wang and Qian, Gao and Ullah, Razi and et al.}, year={2020}, month={Nov}, pages={110947} }
 @article{din_mazhar_wasim_ahmad_bibi_hassan_ali_gang_qian_ullah_et al._2020, title={SARS-CoV-2 microbiome dysbiosis linked disorders and possible probiotics role}, volume={11}, url={http://europepmc.org/abstract/PMC/PMC7657099}, journal={Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie}, author={Din, A and Mazhar, M and Wasim, M and Ahmad, W and Bibi, A and Hassan, A and Ali, N and Gang, W and Qian, G and Ullah, R and et al.}, year={2020}, month={Nov} }
 @article{ali_lin_qing_xiao_din_ali_lian_chen_wen_2020, title={The Role of Agriculture in the Dissemination of Class 1 Integrons, Antimicrobial Resistance, and Diversity of Their Gene Cassettes in Southern China}, volume={11}, url={https://www.mdpi.com/2073-4425/11/9/1014}, DOI={10.3390/genes11091014}, abstractNote={Integrons are hot spots for acquiring gene cassettes from the environment and play a major role in the bacterial evolution and dissemination of antimicrobial resistance (AMR), thus posing a serious threat. There are currently studies on integrons and antibiotic resistance genes; however, the presence and association of integrons in different agricultural crops and their subsequent dissemination and role in AMR have not been reported previously. This study examines the abundance of integrons, their gene cassette diversity in various crop soils, and their role in the dissemination of AMR in the southern region of China. Samples from different agri-crop soil, such as rice (R.S), sugarcane (S.S), citrus (C.S), banana (B.S), agricultural runoff (the point where the runoff of all sites meet (R.O)), and wild (non-agricultural) soil (W.S), were collected. Quantitative PCR was used to determine the abundance of integrons, and clone libraries were constructed to examine the gene cassette arrays. All the tested samples were found positive for Class-I (CL1) integrons and revealed a higher concentration and higher relative abundance of R.S than the others, with the least found at the W.S site. The W.S CL1 cassette arrays were found empty, and no putative conserved domains were found. The R.O was found to contain a high number of gene cassettes with various functions, while the smallest number of gene cassettes was found in the S.S among the crop soils. Most of the gene cassettes presented by the R.O were primarily shared with other sites, and the antibiotic-resistant genes were consistently observed to be dominant. The constructed clone libraries represented a diverse gene cassette array with 16% novel gene cassettes that play a vital role in pathogenesis, transportation, biosynthesis, and AMR. Most resistance-related gene cassettes were associated with the genes encoding resistance to quaternary ammonium compound (QAC) and aminoglycosides. This study highlights the significant differences in the abundance of integrons among various agricultural soils and offers deep insight into the pools of gene cassettes that play a key role in the dissemination of integrons and AMR.}, number={9}, journal={Genes}, publisher={MDPI AG}, author={Ali, Niyaz and Lin, Yinfu and Qing, Zhen and Xiao, Dan and Din, Ahmad Ud and Ali, Izhar and Lian, Tengxiang and Chen, Baoshan and Wen, Ronghui}, year={2020}, month={Aug}, pages={1014} }
 @article{din_hassan_zhu_yin_gregersen_wang_2019, title={Amelioration of TMAO through probiotics and its potential role in atherosclerosis}, volume={103}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-85074583905&partnerID=MN8TOARS}, DOI={10.1007/s00253-019-10142-4}, abstractNote={Atherosclerosis is a major cause of mortalities and morbidities worldwide. It is associated with hyperlipidemia and inflammation, and become chronic by triggering metabolites in different metabolic pathways. Disturbance in the human gut microbiota is now considered a critical factor in the atherosclerosis. Trimethylamine-N-oxide (TMAO) attracts attention and is regarded as a vital contributor in the development of atherosclerosis. TMAO is generated from its dietary precursors choline, carnitine, and phosphatidylcholine by gut microbiota into an intermediate compound known as trimethylamine (TMA), which is then oxidized into TMAO by hepatic flavin monooxygenases. The present review focus on advances in TMAO preventing strategies through probiotics, including, modulation of gut microbiome, metabolomics profile, miRNA, or probiotic antagonistic abilities. Furthermore, possible recommendations based on relevant literature have been presented, which could be applied in probiotics and atherosclerosis-preventing strategies.}, number={23-24}, journal={Applied Microbiology and Biotechnology}, author={Din, A.U. and Hassan, A. and Zhu, Y. and Yin, T. and Gregersen, H. and Wang, G.}, year={2019}, pages={9217–9228} }
 @article{zhu_li_din_hassan_wang_wang_2019, title={Beneficial effects of Enterococcus faecalis in hypercholesterolemic mice on cholesterol transportation and gut microbiota}, volume={103}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-85061713119&partnerID=MN8TOARS}, DOI={10.1007/s00253-019-09681-7}, abstractNote={Hypercholesterolemia plays a critical role in the development of atherosclerosis and cardiovascular diseases. Many works have been reported that gut microbiota could affect hypercholesterolemia through cholesterol metabolism. However, the role of gut microbiota on cholesterol transportation remains unclear. In this study, 8-week-old C57BL/6J mice were fed with high-cholesterol diet to build the hypercholesterolemic mice. Then, the hypercholesterolemic mice got the oral administration of Enterococcus faecalis ATCC19433 at a dose of 10 9  CFU/mL/day or PBS with high-cholesterol diet for 4 weeks. Serum was collected to detect the concentration of total cholesterol (TC). Meanwhile, pathology, histology, real-time polymerase chain reaction, Western blot, and immunofluorescence were used to evaluate the expression of ABCG5 and ABCG8 in the liver and small intestine. We also analyzed the composition of gut microbiota through high-throughput sequencing method. Oral administration of E. faecalis ATCC19433 significantly decreased the concentration of serum cholesterol in hypercholesterolemic mice. Furthermore, E. faecalis ATCC19433 reduced the concentration of liver cholesterol and improved cholesterol by increasing the expression of ABCG5 and ABCG8. Moreover, oral administration of E. faecalis ATCC19433 modulated the composition of gut microbiota and increased the counts of Lactobacillus, Bifidobacterium, and Akkermansia. Our results showed that E. faecalis ATCC19433 could exert hypocholesterolemic effect on hypercholesterolemic mice by improving transporter ABCG5 and ABCG8. E. faecalis ATCC19433 maybe contribute to the transportation of cholesterol potentially and modulate the composition of gut microbiota.}, number={7}, journal={Applied Microbiology and Biotechnology}, author={Zhu, Y. and Li, T. and Din, A.U. and Hassan, A. and Wang, Y. and Wang, G.}, year={2019}, pages={3181–3191} }
 @article{xie_lei_zhang_wang_wen_ye_ud din_jia_apicella_wang_2019, title={Effect of simulated microgravity induced PI3K-nos2b signalling on zebrafish cardiovascular plexus network formation}, volume={87}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-85062372751&partnerID=MN8TOARS}, DOI={10.1016/j.jbiomech.2019.02.019}, abstractNote={Local abnormal angiogenesis and cardiovascular system reorganization have been observed in embryos exposed to a simulated microgravity (SM) environment. In this study, changes in key molecular signals and pathways in cardiovascular development have been investigated under microgravity conditions. In particular, the caudal vein plexus (CVP) network, formed by sprouting angiogenesis has been chosen. Zebrafish embryos were exposed to SM using a ground-based microgravity bioreactor for 24 and 36 h. The SM was observed to have no effect on the zebrafish length, tail width and incubation time whereas it was observed to significantly reduce the heart rate frequency and to promote abnormal development of the CVP network in the embryos. Nitric oxide (NO) content demonstrated that the total proteins in zebrafish embryos were significantly higher in SM than in the control group grown under normal conditions. It was then preliminarily determined how NO signals were involved in SM regulated zebrafish CVP network formation. nos2b MO was injected and CVP network evolution was observed in 36 h post fertilization (hpf) under SM condition. The results showed that the CVP network formation was considerably decreased in the nos2b MO treated group. However, this inhibition of the CVP network development was not observed in control MO group, indicating that nos2b is involved in the SM-regulated vascular development process in zebrafish. Moreover, specific phosphoinositide 3-kinase (PI3K) inhibitors such as LY294002 were also tested on zebrafish embryos under SM condition. This treatment significantly inhibited the formation of zebrafish CVP network. Furthermore, overexpression of nos2b partly rescued the LY294002-caused CVP network failure. Therefore, it can be concluded that SM affects zebrafish CVP network remodeling by enhancing angiogenesis. Additionally, the PI3K-nos2b signaling pathway is involved in this process.}, journal={Journal of Biomechanics}, author={Xie, X. and Lei, D. and Zhang, Q. and Wang, Y. and Wen, L. and Ye, Z. and Ud Din, A. and Jia, D. and Apicella, A. and Wang, G.}, year={2019}, pages={83–92} }
 @article{hassan_din_zhu_zhang_li_wang_luo_wang_2019, title={Updates in understanding the hypocholesterolemia effect of probiotics on atherosclerosis}, volume={103}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-85067831662&partnerID=MN8TOARS}, DOI={10.1007/s00253-019-09927-4}, abstractNote={Atherosclerosis is the major cause of cardiovascular diseases, which are considered the fatal ailment globally. Hypercholesterolaemia plays a critical role in the development of atherosclerosis and cardiovascular diseases. Many studies have been stated that probiotics could affect hypercholesterolemia via cholesterol metabolism. Probiotics are live bacteria which are good for our health when administered orally in high amounts. Recently, many studies have revealed the beneficial effects of the nutritional ingestion of probiotics which can decrease serum cholesterol levels. The aim of this review is, firstly, to explore the hypercholesterolemia effect of how it progresses into atherosclerosis and, secondly, to summarize the hypocholesterolaemia effect of probiotics on atherosclerosis and the up-to-date information on their basic mechanisms. The most important mechanisms responsible for the hypocholesterolemic effect of probiotics are the suppression of the reabsorption of bile acids and inhibition of the intestinal cholesterol absorption. Current studies indicate that numerous mechanisms within the cholesterol metabolism, e.g., ones involving the Niemann-Pick C1-Like 1 protein, 3-hydroxy-3-methylglutaryl-CoA reductase, and 7α- and 27α-hydroxylases, have been recommended where regulation may take place after oral intake of probiotics. However, these mechanisms are still poorly understood. Thus, further studies are required to examine the possible mechanisms, whereby probiotics can be utilized safely and considered for the treatment of hypercholesterolemia.}, number={15}, journal={Applied Microbiology and Biotechnology}, author={Hassan, A. and Din, A.U. and Zhu, Y. and Zhang, K. and Li, T. and Wang, Y. and Luo, Y. and Wang, G.}, year={2019}, pages={5993–6006} }
 @article{najam_shah_mehmood_din_rizwan_ashfaq_shaheen_waseem_2018, title={An overview on the progress and development on metals/non-metal catalyzed cyanation reactions}, volume={469}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-85030533007&partnerID=MN8TOARS}, DOI={10.1016/j.ica.2017.09.058}, abstractNote={This review is based on the analysis of recent developments on cyanation reactions for the synthesis of nitriles (RCN) through transition metal mediated cyanation reaction as well as by using non-metal like iodine (transition metal free system). In this regard, various catalyzed systems were reviewed to find the most efficient method having acceptable reaction system with surely less cost, mild conditions with easy handling. For the synthesis of nitriles; Copper, Palladium, Rhodium, and Iodine-based catalyzed systems have been explored for various aryl halides, arenes, pyridine, quinolone, imidazole, thiophenol, β-keto esters/amides, 2-arylbenzothiazoles etc. These reactions underwent with different time rates having the corresponding nitriles leading to natural products derivative in moderate to good yields.}, journal={Inorganica Chimica Acta}, author={Najam, T. and Shah, S.S.A. and Mehmood, K. and Din, A.U. and Rizwan, S. and Ashfaq, M. and Shaheen, S. and Waseem, A.}, year={2018}, pages={408–423} }
 @article{zang_zou_zhang_xiang_huang_luo_wang_lei_li_song_et al._2016, title={The de-etiolated 1 homolog of arabidopsis modulates the ABA signaling pathway and ABA biosynthesis in rice}, volume={171}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84973621472&partnerID=MN8TOARS}, DOI={10.1104/pp.16.00059}, abstractNote={Abstract}, number={2}, journal={Plant Physiology}, author={Zang, G. and Zou, H. and Zhang, Y. and Xiang, Z. and Huang, J. and Luo, L. and Wang, C. and Lei, K. and Li, X. and Song, D. and et al.}, year={2016}, pages={1259–1276} }