Works (87)

Updated: May 23rd, 2024 05:04

2024 journal article

A Functional RNA-Origami as Direct Thrombin Inhibitor with Fast-acting and Specific Single-Molecule Reversal Agents in vivo model

Molecular Therapy, 5.

Sources: Crossref, NC State University Libraries
Added: May 17, 2024

2024 journal article

Controlled Stiffness of Direct-Write, Near-Field Electrospun Gelatin Fibers Generates Differences in Tenocyte Morphology and Gene Expression

Journal of Biomechanical Engineering, 146(9).

Sources: Crossref, NC State University Libraries
Added: May 17, 2024

2024 journal article

Ultrasoft platelet-like particles stop bleeding in rodent and porcine models of trauma

SCIENCE TRANSLATIONAL MEDICINE, 16(742).

By: K. Nellenbach n, E. Mihalko n, S. Nandi n, D. Koch n, J. Shetty*, L. Moretti*, J. Sollinger n, N. Moiseiwitsch n ...

UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: April 22, 2024

2024 journal article

Utilizing multiscale engineered biomaterials to examine <scp>TGF‐β</scp>‐mediated myofibroblastic differentiation

Wound Repair and Regeneration, 3.

By: A. Simpson n, A. Krissanaprasit n, D. Chester n, C. Koehler n, T. LaBean n & A. Brown n

author keywords: biomaterials; DNA origami; fibrosis; mechanotransduction; microgel; TGF-beta
TL;DR: It is found that highly viscous materials with non-fixed TGF-β-R spacing promoted increased TGF-β signalling and myofibroblastic differentiation, likely due to the ability of cells to better cluster receptors on these surfaces. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: March 18, 2024

2023 journal article

Biomaterials for treating sepsis-induced thromboinflammation

Frontiers Media SA.

By: H. Lutz & A. Brown

Sources: Crossref, NC State University Libraries
Added: May 17, 2024

2023 journal article

Characteristics of Fetal Wound Healing and Inspiration for Pro-healing Materials

Biomedical Materials & Devices, 2(1), 158–167.

By: S. Pandit n, K. Nellenbach n & A. Brown n

Sources: Crossref, NC State University Libraries
Added: May 17, 2024

2023 journal article

Heparin Affinity-Based IL-4 Delivery to Modulate Macrophage Phenotype and Endothelial Cell Activity In Vitro

ACS Applied Materials & Interfaces, 15(23), 27457–27470.

By: F. Zhang n, J. Gluck n, A. Brown n, D. Zaharoff n & M. King n

author keywords: gelatin methacryloyl; heparin; interleukin4; hydrogel; macrophage; endothelial cell; immunomodulation
MeSH headings : Interleukin-4 / pharmacology; Heparin / pharmacology; Heparin / metabolism; Macrophages / metabolism; Phenotype; Hydrogels / pharmacology; Hydrogels / metabolism
TL;DR: A gelatin-heparin-methacrylate hydrogel is engineered that gradually releases interleukin-4 (IL-4), a cytokine that modulates macrophages to adopt the M2 phenotype and synergistically downregulated M1 gene expression and upregulated M2 gene expression within 48 h of in vitro cell culture. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref, NC State University Libraries, ORCID
Added: June 26, 2023

2023 journal article

Influence of Fibrinogen Concentrate on Neonatal Clot Structure When Administered Ex Vivo After Cardiopulmonary Bypass

Anesthesia & Analgesia, 137(3), 682–690.

By: N. Moiseiwitsch n, K. Nellenbach n, L. Downey*, D. Boorman*, A. Brown n & N. Guzzetta*

TL;DR: It is suggested that FC is effective in restoring structural fibrin clot properties after CPB, and further studies after the administration of FC in vivo are needed to validate this hypothesis. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref
Added: September 5, 2023

2023 journal article

Microparticle Hydrogel Material Properties Emerge from Mixing-Induced Homogenization in a Poly(ethylene glycol) and Dextran Aqueous Two-Phase System

Macromolecules, 56(21), 8518–8528.

By: T. Tigner*, G. Scull n, A. Brown n & D. Alge*

UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: December 4, 2023

2023 journal article

Molecular Engineering of Cyclic Azobenzene‐Peptide Hybrid Ligands for the Purification of Human Blood Factor VIII via Photo‐Affinity Chromatography

Advanced Functional Materials, 33(14).

By: R. Prodromou n, B. Moore n, W. Chu n, H. Deal n, A. San Miguel n, A. Brown n, M. Daniele n, V. Pozdin*, S. Menegatti n

author keywords: affinity ligands; azobenzene-peptide hybrids; biomolecular recognition; factor VIII; photo-affinity chromatography
TL;DR: The cyclic azobenzene‐peptide ligands introduced in this report demonstrate a novel route integrating gentle operational conditions in a rapid and efficient bioprocess for the purification of life‐saving biotherapeutics. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref, NC State University Libraries, ORCID
Added: February 20, 2023

2023 journal article

Recent Advances in Blood Cell-Inspired and Clot-Targeted Thrombolytic Therapies

Journal of Tissue Engineering and Regenerative Medicine, 2023, 1–14.

By: A. Sheridan n & A. Brown n

Ed(s): C. Kuo

TL;DR: This review will highlight potential thrombus targeting mechanisms as well as recent advances in thrombolytic therapies which utilize blood-cells and clotting proteins to effectively target and lyse clots. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: March 27, 2023

2023 journal article

Simple design for membrane-free microphysiological systems to model the blood-tissue barriers

Organs-on-a-Chip, 5, 100032.

By: B. Young n, H. Deal n, G. Rusch n, V. Pozdin*, A. Brown n & M. Daniele n

Sources: Crossref, NC State University Libraries
Added: May 17, 2024

2023 journal article

Wound‐triggered shape change microgels for the development of enhanced biomimetic function platelet‐like particles

Journal of Biomedical Materials Research Part A, 112(4), 613–624.

By: E. Chee n, E. Mihalko*, K. Nellenbach n, J. Sollinger n, K. Huang n, M. Hon n, S. Pandit n, K. Cheng n, A. Brown n

author keywords: fibrin; hemostasis; synthetic platelet; trauma; wound healing
TL;DR: Thrombin-sensitive PLPs are created that exhibit a wound-triggered shape change, induce significant clot retraction following exposure to thrombin and promote hemostasis and healing in vivo after trauma. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: November 6, 2023

2022 journal article

A Unique Crustacean-Based Chitin Platform to Reduce Self-Aggregation of Polysaccharide Nanofibers

Fibers, 10(10), 87.

author keywords: polysaccharide nanofibers; crustacean shells; chitin; cellulose; self-aggregation; clotting; foams
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Web Of Science, Crossref, NC State University Libraries, ORCID
Added: October 19, 2022

2022 journal article

Biomaterials for Hemostasis

Annual Review of Biomedical Engineering, 24(1), 111–135.

By: A. Simpson n, A. Shukla* & A. Brown n

author keywords: hemostasis; trauma; clotting; sealant; synthetic blood product; layer-by-layer assembly
MeSH headings : Biocompatible Materials; Hemorrhage; Hemostasis; Hemostatics; Humans
TL;DR: This review article provides an overview of classes of materials used for topical hemostat and newer developments in the area of injectable hemostats for use in emergency medicine. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref, NC State University Libraries
Added: July 11, 2022

2022 journal article

COVID‐19 patient fibrinogen produces dense clots with altered polymerization kinetics, partially explained by increased sialic acid

Journal of Thrombosis and Haemostasis, 20(12), 2909–2920.

author keywords: coagulopathy; COVID-19; fibrinogen; sialic acid; thrombosis
MeSH headings : Humans; Fibrinogen / metabolism; Fibrin / chemistry; N-Acetylneuraminic Acid; COVID-19; Polymerization; Neuraminidase; SARS-CoV-2; Fibrinolysis; Thrombosis / metabolism; Hemostatics
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: October 17, 2022

2022 journal article

Effects of sterilization methods on gelatin methacryloyl hydrogel properties and macrophage gene expression in vitro

Biomedical Materials, 18(1), 015015.

By: F. Zhang n, G. Scull n, J. Gluck n, A. Brown n & M. King n

author keywords: gelatin methacryloyl; hydrogel; sterilization; macrophage phenotype; gene expression
MeSH headings : Hydrogels; Macrophages
TL;DR: It was found that the sterilization methods only marginally altered the hydrogel morphology, swelling behavior and elastic modulus, but significantly impacted macrophage gene expression within 48 h and over 7 d in vitro, suggesting that not only the sterility and hydrogels properties, such as material destruction and degradation caused by temperature and moisture, should be taken into consideration. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, Crossref, NC State University Libraries, ORCID
Added: November 22, 2022

2022 journal article

Elucidating the combinatorial effect of substrate stiffness and surface viscoelasticity on cellular phenotype

Journal of Biomedical Materials Research Part A, 110(6), 1224–1237.

By: D. Chester n, V. Lee n, P. Wagner n, M. Nordberg n, M. Fisher n & A. Brown n

author keywords: loss tangent; mechanotransduction; microgel; viscoelasticity
MeSH headings : Biocompatible Materials / chemistry; Hydrogels / chemistry; Hydrogels / pharmacology; Mechanotransduction, Cellular / physiology; Phenotype; Viscosity
TL;DR: A novel composite hydrogel system capable of decoupling and individually controlling both the bulk stiffness and surface viscoelasticity of the material by combining polyacrylamide gels with microgel thin films is characterized, allowing for a greater understanding of cellular mechanotransduction mechanisms than previously possible through current model material platforms. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref, NC State University Libraries
Added: February 7, 2022

2022 journal article

Inhalable dry powder mRNA vaccines based on extracellular vesicles

Matter, 5(9), 2960–2974.

By: K. Popowski n, A. Moatti n, G. Scull n, D. Silkstone n, H. Lutz n, B. López de Juan Abad n, A. George n, E. Belcher n ...

TL;DR: The results suggest that extracellular vesicles can serve as an inhaled mRNA drug-delivery system that is superior to synthetic liposomes. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref, ORCID
Added: October 17, 2022

2022 journal article

On-demand, remote and lossless manipulation of biofluid droplets

Materials Horizons, 9(11), 2863–2871.

By: W. Wang n, J. Sun*, S. Vallabhuneni n, B. Pawlowski*, H. Vahabi*, K. Nellenbach n, A. Brown n, F. Scholle n, J. Zhao*, A. Kota n

MeSH headings : Humans; Pandemics / prevention & control; Saliva; Disease Outbreaks; Point-of-Care Systems; Health Personnel
TL;DR: This work presents biofluid manipulators, which allow on-demand, remote and lossless manipulation of virtually any liquid droplet, and envision that these manipulators will not only reduce manual operations and minimize exposure to infectious agents, but also pave the way for developing inexpensive, simple and portable robotic systems, which can allow point-of-care operations, particularly in developing nations. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref
Added: September 19, 2022

2022 journal article

Plasma-Induced Diallyldimethylammonium Chloride Antibacterial Hernia Mesh

ACS Applied Bio Materials, 5(12), 5645–5656.

By: Z. Wang n, H. Hamedi n, F. Zhang n, A. El-Shafei n, A. Brown n, J. Gluck n, M. King n

author keywords: hernia mesh; polypropylene; plasma; surface grafting; antibacterial; fibroblast
MeSH headings : Humans; Surgical Mesh / adverse effects; Polypropylenes; Hernia, Ventral / drug therapy; Anti-Bacterial Agents / pharmacology
TL;DR: A polypropylene mesh with antibacterial properties was developed and grafted onto the mesh surface using pentaerythritol tetraacrylate as the cross-linker since it is able to engage all four functional groups to form a high-density cross-linked network. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, Crossref, NC State University Libraries, ORCID
Added: November 30, 2022

2022 article

Platelet-mimicking procoagulant nanoparticles: Potential strategies for mitigating blood shortages

Nellenbach, K., & Brown, A. C. (2022, April 19). JOURNAL OF THROMBOSIS AND HAEMOSTASIS, Vol. 4.

By: K. Nellenbach n & A. Brown n

MeSH headings : Blood Platelets; Humans; Nanoparticles; Platelet Activation
TL;DR: A new study has demonstrated the creation of plateletmimicking procoagulant nanoparticles (PPNs) that have advanced platelet function compared with prior designs, and the implications for these new findings in the field of synthetic platelet design are discussed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: May 2, 2022

2022 journal article

Restoring Carboxylates on Highly Modified Alginates Improves Gelation, Tissue Retention and Systemic Capture

Acta Biomaterialia, 138, 208–217.

By: C. Moody n, A. Brown n, N. Massaro n, A. Patel n, P. Agarwalla n, A. Simpson n, A. Brown n, H. Zheng n, J. Pierce n, Y. Brudno n

author keywords: Alginate; Click chemistry; Bioconjugation; Ionic cross-linking; Drug capture
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref, NC State University Libraries, ORCID
Added: October 31, 2021

2021 journal article

Differential sialic acid content in adult and neonatal fibrinogen mediates differences in clot polymerization dynamics

BLOOD ADVANCES, 5(23), 5202–5214.

By: K. Nellenbach, A. Kyu, N. Guzzetta & A. Brown

MeSH headings : Adult; Fibrin; Fibrinogen; Fibrinopeptide A; Humans; Infant, Newborn; N-Acetylneuraminic Acid; Polymerization
Sources: Web Of Science, NC State University Libraries
Added: January 3, 2022

2021 journal article

Differential sialic acid content in adult and neonatal fibrinogen mediates differences in clot polymerization dynamics

Blood Advances, 5(23), 5202–5214.

By: K. Nellenbach n, A. Kyu n, N. Guzzetta* & A. Brown n

Sources: Crossref, NC State University Libraries
Added: May 17, 2024

2021 article

Ex Vivo and In Vivo Evaluation of Fibrinogen Concentrate to Mitigate Post-Surgical Bleeding in Neonates

Moiseiwitsch, N., Nellenbach, K. A., Guzzetta, N. A., Brown, A. C., & Downey, L. (2021, November 23). BLOOD, Vol. 138.

By: N. Moiseiwitsch n, K. Nellenbach n, N. Guzzetta*, A. Brown n & L. Downey*

UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: September 6, 2022

2021 journal article

Fibrin-modulating nanogels for treatment of disseminated intravascular coagulation

Blood Advances, 5(3), 613–627.

By: E. Mihalko n, M. Sandry n, N. Mininni n, K. Nellenbach n, H. Deal n, M. Daniele n, K. Ghadimi*, J. Levy*, A. Brown n

MeSH headings : Disseminated Intravascular Coagulation / drug therapy; Fibrin; Humans; Microcirculation; Nanogels; Thrombosis / drug therapy
TL;DR: In vivo and in vivo, tPA-FSNs decreased the presentation of multiorgan microthrombi, recovered platelet counts, and improved bleeding outcomes in a DIC rodent model, demonstrating that a fibrinolytic agent loaded into fibrin-targeting nanogels could improve DIC outcomes. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
5. Gender Equality (Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: March 22, 2021

2021 journal article

Fibrin‐specific <scp>poly(N‐isopropylacrylamide)</scp> nanogels for targeted delivery of tissue‐type plasminogen activator to treat thrombotic complications are well tolerated in vivo

Bioengineering & Translational Medicine, 7(2).

author keywords: disseminated intravascular coagulation; fibrin; nanogels; pNIPAM; targeted delivery; thrombosis; tissue plasminogen activator
TL;DR: The therapeutic efficacy of targeted fibrin‐specific nanogel (FSN) design for targeted delivery of tPA to treat thrombotic complications is demonstrated and key translational parameters for tPA‐FSN therapeutics are evaluated, including optimal tPA •FSN dosage in a DIC rodent model and safety of intravenous tPA•FSN therapies. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
5. Gender Equality (Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: December 20, 2021

2021 journal article

Modeling and Parameter Subset Selection for Fibrin Polymerization Kinetics with Applications to Wound Healing

Bulletin of Mathematical Biology, 83(5).

By: K. Pearce n, K. Nellenbach n, R. Smith n, A. Brown n & M. Haider n

author keywords: Wound healing; Fibrin polymerization; Kinetics model; Parameter identifiability; Subset selection
MeSH headings : Animals; Cells, Cultured; Fibrin / metabolism; Humans; Kinetics; Models, Biological; Polymerization; Wound Healing
TL;DR: The findings demonstrate the high degree of information within a single fibrin accumulation curve, and a tailored model and parameter subset selection approach for improving optimization and reducing model complexity in the context of polymerization experiments. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref, NC State University Libraries, ORCID
Added: March 23, 2021

2021 journal article

Multivalent Aptamer‐Functionalized Single‐Strand RNA Origami as Effective, Target‐Specific Anticoagulants with Corresponding Reversal Agents

Advanced Healthcare Materials, 10(11).

By: A. Krissanaprasit n, C. Key n, K. Froehlich n, S. Pontula, E. Mihalko n, D. Dupont*, E. Andersen*, J. Kjems*, A. Brown n, T. LaBean n

author keywords: anticoagulants; aptamers; direct thrombin inhibitors; nucleic acids; reversal agents; RNA nanotechnology; RNA origami
MeSH headings : Anticoagulants / pharmacology; Aptamers, Nucleotide / pharmacology; Blood Coagulation; RNA / pharmacology; Thrombin
TL;DR: Compared to current commercial intravenous anticoagulants, RNA origami‐based molecules show promise as safer alternatives with rapid activity switching for future therapeutic applications. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref, NC State University Libraries
Added: June 10, 2021

2021 journal article

Neonatal coagulopathies: A review of established and emerging treatments

Experimental Biology and Medicine, 246(12), 1447–1457.

By: N. Moiseiwitsch n & A. Brown n

author keywords: Neonatal; coagulopathy; developmental hemostasis; transfusion
MeSH headings : Animals; Blood Coagulation Disorders / etiology; Blood Coagulation Disorders / therapy; Hemorrhage / etiology; Hemorrhage / therapy; Hemostasis / physiology; Humans; Infant, Newborn; Infant, Newborn, Diseases / etiology; Infant, Newborn, Diseases / therapy
TL;DR: This mini-review examines the current understanding of neonatal hemostatic balance and treatment of Neonatal coagulopathies, with particular emphasis on emerging treatment methods and areas in need of further investigative efforts. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
5. Gender Equality (Web of Science)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: July 12, 2021

2021 journal article

Platelet‐like</scp> particles reduce <scp>coagulopathy‐related</scp> and neuroinflammatory pathologies <scp>post‐experimental</scp> traumatic brain injury

Journal of Biomedical Materials Research Part B: Applied Biomaterials, 109(12), 2268–2278.

By: J. Todd*, V. Bharadwaj*, K. Nellenbach n, S. Nandi n, E. Mihalko n, C. Copeland*, A. Brown n, S. Stabenfeldt*

author keywords: blood-brain barrier; coagulopathy; neuroinflammation; permeability; platelet-like particles; traumatic brain injury
MeSH headings : Animals; Blood Platelets / metabolism; Blood-Brain Barrier / metabolism; Brain Injuries, Traumatic / complications; Brain Injuries, Traumatic / drug therapy; Brain Injuries, Traumatic / metabolism; Disease Models, Animal; Mice; Microglia / metabolism
TL;DR: Results suggest that PLP-treatment addressed acute hypocoagulation and decreased BBB permeability followed by decreased neuroinflammation and fold-change tissue loss by 7 days post-injury, indicating that PLPs could be a potential therapeutic modality for TBI. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: June 21, 2021

2021 journal article

Synthesis of sonicated fibrin nanoparticles that modulate fibrin clot polymerization and enhance angiogenic responses

Colloids and Surfaces B: Biointerfaces, 204, 111805.

By: C. Roosa n, I. Muhamed n, A. Young n, K. Nellenbach n, M. Daniele n, F. Ligler n, A. Brown n

author keywords: Fibrin; Nanoparticles; Wound healing; Regenerative medicine; Fibrin sealant
MeSH headings : Fibrin; Fibrin Tissue Adhesive; Nanoparticles; Polymerization; Wound Healing
TL;DR: It is shown that SFBNs may be a promising wound healing therapy that can be easily produced and delivered in a flowable formulation and support cell migration in an in vitro angiogenic sprouting assay, relevant for wound healing. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref, NC State University Libraries
Added: July 19, 2021

2021 journal article

Synthetic Platelet Microgels Containing Fibrin Knob B Mimetic Motifs Enhance Clotting Responses

Advanced Therapeutics, 4(5).

By: S. Nandi n, E. Mihalko n, K. Nellenbach n, M. Castaneda n, J. Schneible n, M. Harp*, H. Deal n, M. Daniele n ...

author keywords: bleeding; Clotting; fibrin; hemostasis; microgel
TL;DR: Soft microgels decorated with the peptide AHRPYAAK are found to significantly increase clot density in vitro and decrease bleeding in a rodent trauma model in vivo, indicating that FAMCYs are capable of recapitulating the platelet‐mimetic properties of previous designs while utilizing a less costly, more translational design. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: April 5, 2021

2020 journal article

Biomimetic antimicrobial material strategies for combating antibiotic resistant bacteria

Biomaterials Science, 8(4), 1089–1100.

By: E. Chee n & A. Brown n

MeSH headings : Animals; Anti-Bacterial Agents / chemistry; Anti-Bacterial Agents / pharmacology; Bacteria / drug effects; Bacteria / growth & development; Bacterial Infections / drug therapy; Biomimetic Materials / chemistry; Biomimetic Materials / pharmacology; Drug Resistance, Bacterial / drug effects; Humans; Technology, Pharmaceutical
TL;DR: This review will discuss current antimicrobial strategies and present an overview on biomimetic antimicrobial material-based strategies for combating antibiotic resistant bacteria. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref
Added: March 16, 2020

2020 journal article

Comparison of Neonatal and Adult Fibrin Clot Properties between Porcine and Human Plasma

Anesthesiology, 132(5), 1091–1101.

By: K. Nellenbach n, S. Nandi n, A. Kyu n, S. Sivadanam n, N. Guzzetta n & A. Brown n

UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: May 17, 2024

2020 journal article

Development of Novel Microenvironments for Promoting Enhanced Wound Healing

Current Tissue Microenvironment Reports, 1(3), 73–87.

By: G. Scull n & A. Brown n

UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: May 17, 2024

2020 journal article

Effect of surface interactions on the settlement of particles on a sinusoidally corrugated substrate

RSC Advances, 10(19), 11348–11356.

By: S. Erramilli n, T. Neumann n, D. Chester n, M. Dickey n, A. Brown n & J. Genzer n

Sources: Crossref, NC State University Libraries, ORCID
Added: March 21, 2020

2020 journal article

Fibrin‐based biomaterial systems to enhance anterior cruciate ligament healing

MEDICAL DEVICES & SENSORS, 4(1).

By: G. Scull n, M. Fisher n & A. Brown n

UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: May 17, 2024

2020 journal article

Microphysiological systems for the modeling of wound healing and evaluation of pro-healing therapies

Journal of Materials Chemistry B, 8(32), 7062–7075.

By: H. Deal n, A. Brown n & M. Daniele n

MeSH headings : Animals; Cell Culture Techniques; Cell Line; Cell Movement; Cellular Microenvironment; Hemostasis / drug effects; Humans; Hydrogels / chemistry; Hydrogels / metabolism; Macrophages / metabolism; Microfluidics; Models, Animal; Skin; Stereolithography; Wound Healing / drug effects
TL;DR: This review provides a brief overview of the wound healing process and in vivo assays, and critically review the current state of microphysiological systems and supporting technologies for modelling and studying wound healing. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref, NC State University Libraries
Added: September 7, 2020

2020 journal article

Nanosilver composite pNIPAm microgels for the development of antimicrobial platelet‐like particles

Journal of Biomedical Materials Research Part B: Applied Biomaterials, 108(6), 2599–2609.

By: E. Chee n, S. Nandi n, K. Nellenbach n, E. Mihalko n, D. Snider n, L. Morrill n, A. Bond n, E. Sproul n ...

Contributors: E. Chee n, S. Nandi n, K. Nellenbach n, E. Mihalko n, D. Snider n, L. Morrill n, A. Bond n, E. Sproul n ...

author keywords: antimicrobial; microgels; nanosilver; platelet-mimetic; wound healing
MeSH headings : Acrylic Resins / chemistry; Animals; Anti-Infective Agents / chemistry; Anti-Infective Agents / pharmacology; Bacterial Infections / drug therapy; Bacterial Infections / microbiology; Blood Platelets; Clot Retraction; Colony-Forming Units Assay; Fibrin / chemistry; Fibrin / immunology; Gels; Hemorrhage / drug therapy; Hemostasis / drug effects; Liver / injuries; Male; Metal Nanoparticles; Mice; Mice, Inbred C57BL; Microgels; Silver / administration & dosage; Silver / chemistry; Wound Healing
TL;DR: It is found that nanosilver incorporation does not affect base PLP performance while bestowing significant antimicrobial activity and enhancing infected wound healing outcomes, and Ag-PLPs have great promise for treating hemorrhage and improving healing following trauma. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref, ORCID
Added: March 16, 2020

2020 journal article

Neonatal Fibrin Scaffolds Promote Enhanced Cell Adhesion, Migration, and Wound Healing In Vivo Compared to Adult Fibrin Scaffolds

Cellular and Molecular Bioengineering, 13(5), 393–404.

By: K. Nellenbach n, S. Nandi n, C. Peeler n, A. Kyu n & A. Brown n

TL;DR: In vivo significantly smaller wound areas and greater epidermal thickness were observed when wounds were treated with neonatal fibrin compared to adultfibrin or a saline control, indicating that fibr in scaffolds sourced from neonatal plasma could improve healing outcomes compared to scaffoldsourced from adult plasma. (via Semantic Scholar)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: June 15, 2020

2020 journal article

Platelet-like particles improve fibrin network properties in a hemophilic model of provisional matrix structural defects

Journal of Colloid and Interface Science, 577, 406–418.

author keywords: Fibrin; Biomimetic; Platelet; Hemophilia; Biomaterials
MeSH headings : Blood Coagulation; Blood Platelets; Fibrin; Plasma; Wound Healing
TL;DR: PLPs improved clot network density, force generation, and stiffness, and promoted fibroblast migration within an in vitro model of early wound healing under hemophilic conditions, indicating that PLPs could provide a biomimetic platform for improving wound healing events in disease conditions that cause deficient fibrin network formation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 24, 2020

2020 journal article

Ultrasonic Microplotting of Microgel Bioinks

ACS Applied Materials & Interfaces, 12(42), 47309–47319.

By: D. Chester n, P. Theetharappan n, T. Ngobili n, M. Daniele n & A. Brown n

author keywords: microgel; microplotting; patterned films; layer-by-layer fabrication; bioink
MeSH headings : Ink; Microgels / analysis; Molecular Structure; Particle Size; Surface Properties; Tissue Scaffolds / chemistry; Ultrasonic Waves
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref, NC State University Libraries
Added: December 21, 2020

2020 journal article

Ultrasound Enhanced Synthetic Platelet Therapy for Augmented Wound Repair

ACS Biomaterials Science & Engineering, 6(5), 3026–3036.

By: S. Nandi n, K. Mohanty n, K. Nellenbach n, M. Erb n, M. Muller n & A. Brown n

author keywords: biomimetic; fibrin; wound healing clot retraction; artificial platelet
MeSH headings : Blood Coagulation; Blood Platelets; Clot Retraction; Fibrin; Wound Healing
TL;DR: Application of ultrasound in combination with a subtherapeutic dosage of PLPs resulted in increased clot density and stiffness, improved fibroblast migration in vitro and increased epidermal thickness and angiogenesis in vivo, indicating that this combination therapy has potential to facilitate multiphase pro-healing outcomes. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref
Added: June 8, 2020

2019 journal article

Biomimetic Strategies To Treat Traumatic Brain Injury by Leveraging Fibrinogen

Bioconjugate Chemistry, 30(7), 1951–1956.

By: A. Brown n, E. Lavik* & S. Stabenfeldt*

MeSH headings : Biomimetics; Blood-Brain Barrier; Brain Injuries, Traumatic / therapy; Fibrinogen / metabolism; Humans
TL;DR: This work deconstructs what components of fibrinogen may be beneficial as well as, potentially harmful, following TBI and extrapolate this to biomimetic approaches to treat bleeding and trauma that may, also, lead to better outcomesFollowing TBI. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 12, 2019

2019 journal article

Clot Structure and Implications for Bleeding and Thrombosis

Seminars in Thrombosis and Hemostasis, 46(01), 096–104.

By: E. Mihalko n & A. Brown n

author keywords: fibrin; clot structure; atomic force microscopy; fibrinolysis; thrombosis
MeSH headings : Fibrin / metabolism; Fibrinolysis; Hemorrhage / metabolism; Hemorrhage / pathology; Humans; Thrombosis / metabolism; Thrombosis / pathology
TL;DR: Known factors that contribute to changes in clot structure and the presence of structural clot changes in various disease states are examined to provide valuable information in forming therapeutic strategies for disease states where alterations in clotructure are observed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref, NC State University Libraries
Added: February 27, 2020

2019 journal article

Development of Biomimetic Antimicrobial Platelet-Like Particles Comprised of Microgel Nanogold Composites

Regenerative Engineering and Translational Medicine, 6(3), 299–309.

By: E. Sproul n, S. Nandi n, E. Chee n, S. Sivadanam n, B. Igo n, L. Schreck*, A. Brown n

author keywords: Synthetic platelet; Fibrin; Hemostatic material; Trauma; Antimicrobial material; Microgels
TL;DR: These results demonstrate NGCs fabricated through noncovalent and covalent methods retain deformability necessary for clot collapse and exhibit some antimicrobial potential, and are promising materials for preventing blood loss and infection following traumatic injury. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: October 12, 2020

2019 journal article

Fibrin Nanoparticles Coupled with Keratinocyte Growth Factor Enhance the Dermal Wound-Healing Rate

ACS Applied Materials & Interfaces, 11(4), 3771–3780.

By: I. Muhamed n, E. Sproul n, F. Ligler n & A. Brown n

author keywords: fibrin nanoparticles; tissue regeneration; cell migration; microfluidics; wound healing; biomaterials; cell migration; in vitro 3D wound model and in vivo dermal wound model
MeSH headings : Animals; Biocompatible Materials / chemistry; Cell Movement / drug effects; Fibrin / chemistry; Fibroblast Growth Factor 7 / chemistry; Fibroblast Growth Factor 7 / therapeutic use; Mice; Microfluidics; Nanoparticles / chemistry; Wound Healing / drug effects
TL;DR: FBNs described here are constructed from physiologically relevant fibrin concentrations that support new tissue and dermal wound scaffold formation when coupled with growth factors and can be potentially administered with other tissue-healing factors and inflammatory mediators to improve wound- healing outcomes. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref
Added: February 18, 2019

2019 article

Maintaining Hemostatic Balance in Treating Disseminated Intravascular Coagulation

Brown, A. C., & Levy, J. H. (2019, September). ANESTHESIOLOGY, Vol. 131, pp. 459–461.

By: A. Brown n & J. Levy n

MeSH headings : Animals; Blood Coagulation Factors; Disseminated Intravascular Coagulation; Hemostasis; Hemostatics; Swine
TL;DR: It is demonstrated that following experimental animal trauma, administration of prothrombin complex concentrates, either alone or in combination with fibrinogen concentrate, can potentially induce hypercoagulability, but coadministration of antithrombin mitigated this effect. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
5. Gender Equality (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: September 23, 2019

2019 journal article

Mechanical properties of tissue formed in vivo are affected by 3D-bioplotted scaffold microarchitecture and correlate with ECM collagen fiber alignment

Connective Tissue Research, 61(2), 190–204.

By: P. Huebner n, P. Warren n, D. Chester n, J. Spang*, A. Brown n, M. Fisher n, R. Shirwaiker n

author keywords: 3D printing; atomic force microscopy; elastic modulus; tissue engineering; tissue scaffolds
MeSH headings : Animals; Bioprinting; Collagen / chemistry; Extracellular Matrix / chemistry; Male; Polyesters / chemistry; Printing, Three-Dimensional; Rats; Rats, Sprague-Dawley; Tissue Engineering; Tissue Scaffolds / chemistry
TL;DR: The findings demonstrate the significant effects of 3D-bioplotted scaffold microarchitectures in the organization and sub-tissue-level mechanical properties of ECM in vivo. (via Semantic Scholar)
UN Sustainable Development Goal Categories
5. Gender Equality (Web of Science)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 26, 2019

2019 journal article

Platelet-like particles dynamically stiffen fibrin matrices and improve wound healing outcomes

Biomaterials Science, 7(2), 669–682.

By: S. Nandi n, E. Sproul n, K. Nellenbach n, M. Erb n, L. Gaffney n, D. Freytes n, A. Brown n

MeSH headings : Animals; Biomimetic Materials / chemistry; Blood Platelets / metabolism; Blood Platelets / physiology; Fibrin / chemistry; Fibrin / pharmacology; Humans; Mechanical Phenomena; Mice; Platelet Activation / drug effects; Skin / drug effects; Skin / injuries; Wound Healing / drug effects
TL;DR: The utility of PLPs for enhancing wound repair is demonstrated and the role of native platelet-mediated clot retraction in wound healing is provided, to provide insight into the role in vitro and in vivo models of wound healing. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref, NC State University Libraries
Added: February 18, 2019

2019 article

Wound Healing and the Host Response in Regenerative Engineering

ENCYCLOPEDIA OF BIOMEDICAL ENGINEERING, VOL 1, pp. 707–718.

By: D. Chester n, E. Marrow n, M. Daniele n & A. Brown n

TL;DR: The purpose of this book chapter is to highlight the cellular processes and bioactive agents that encompass the wound healing process and to discuss how these processes change in the presence of a biomaterial. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: April 14, 2020

2018 journal article

Analysis of the structural and mechanical effects of procoagulant agents on neonatal fibrin networks following cardiopulmonary bypass

Journal of Thrombosis and Haemostasis, 16(11), 2159–2167.

By: K. Nellenbach n, N. Guzzetta* & A. Brown n

author keywords: activated factor VII; cardiopulmonary bypass; fibrin; neonatal coagulation; prothrombin complex concentrate; transfusion
MeSH headings : Blood Coagulation; Blood Coagulation Factors / therapeutic use; Blood Coagulation Tests; Blood Transfusion; Cardiopulmonary Bypass; Coagulants / therapeutic use; Factor VIIa / therapeutic use; Fibrin / chemistry; Fibrinogen / chemistry; Fibrinolysis; Hemorrhage; Hemostatics / therapeutic use; Humans; Infant, Newborn; Neonatology; Recombinant Proteins / therapeutic use; Risk
TL;DR: Compared neonatal clots formed with cryoprecipitate (SOC) to two procoagulant therapies, which resulted in clots with increased stiffness and decreased fibrinolytic properties may be a viable alternative to SOC treatment for neonatal bleeding. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries, Web Of Science
Added: December 3, 2018

2018 journal article

Biomimetic Microgels with Controllable Deformability Improve Healing Outcomes

Advanced Biosystems, 2(10).

By: E. Sproul n, S. Nandi n, C. Roosa n, L. Schreck* & A. Brown n

author keywords: biomimetic; fibrin; microgel; synthetic platelet; wound healing
TL;DR: Hollow PLPs with low degrees of shell crosslinking improve some wound healing outcomes in vivo, recapitulating the clot retraction function of platelets, while other particle types do not. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref
Added: October 29, 2018

2018 journal article

Enhancing clot properties through fibrin-specific self-cross-linked PEG side-chain microgels

COLLOIDS AND SURFACES B-BIOINTERFACES, 166, 89–97.

By: N. Welsch*, A. Brown n, T. Barker* & L. Lyon*

author keywords: Oligoethylene glycol-based microgels; Platelet-like particles; Fibrin clot; Fibrin-targeting antibodies; Elastic modulus; Permeability
MeSH headings : Elastic Modulus; Fibrin / chemistry; Polyethylene Glycols / chemistry; Polymers / chemistry
TL;DR: PEG-PLP-reinforced fibrin clots exhibit higher degrees of stiffness as demonstrated by the significantly increased average Younǵs modulus, as measured by AFM force spectroscopy. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2018 article

Fibrin biomaterials for tissue regeneration and repair

PEPTIDES AND PROTEINS AS BIOMATERIALS FOR TISSUE REGENERATION AND REPAIR, pp. 151–173.

By: E. Sproul n, S. Nandi n & A. Brown n

TL;DR: This chapter first provides an overview of the structure and function of fibrin(ogen) and details the role offibrin-cell interactions in wound repair and the design and use of fibin-based materials for promoting tissue repair. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2018 journal article

Interdigitated microelectronic bandage augments hemostasis and clot formation at low applied voltage in vitro and in vivo

Lab on a Chip, 18(19), 2985–2993.

By: E. Hardy*, Y. Wang*, S. Iyer*, R. Mannino*, Y. Sakurai*, T. Barker*, T. Chi*, Y. Youn* ...

MeSH headings : Blood Coagulation; Electricity; Hemostasis; Humans; Microtechnology / instrumentation
TL;DR: A novel surface treatment using an interdigitated microelectronic device that creates rapid hemostasis in both in vitro and in vivo bleeding models with low applied voltages is reported, representing a new and novel class of hemostatic agents that are electrically-based. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref
Added: November 19, 2018

2018 journal article

Study of Poly(<i>N</i>-isopropylacrylamide-<i>co</i>-acrylic acid) (pNIPAM) Microgel Particle Induced Deformations of Tissue-Mimicking Phantom by Ultrasound Stimulation

Langmuir, 34(4), 1457–1465.

By: A. Joshi*, S. Nandi n, D. Chester n, A. Brown n & M. Muller n

MeSH headings : Acrylates / chemistry; Acrylic Resins / chemistry; Drug Delivery Systems; Gels / chemistry; Polymers / chemistry
TL;DR: It is demonstrated in tissue-mimicking phantoms that using ultrasound insonification causes deformations of ULC microgel particles, and increasing the degree of microgel cross-linking reduces the magnitude of the deformation and increases the optimal concentration required to achieve the largest amount of deformation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2018 journal article

Targeted Treatment of Ischemic and Fibrotic Complications of Myocardial Infarction Using a Dual-Delivery Microgel Therapeutic

ACS Nano, 12(8), 7826–7837.

By: E. Mihalko n, K. Huang n, E. Sproul n, K. Cheng n & A. Brown n

author keywords: microgels; myocardial infarction; fibrin; fibrinolytic; fibrosis; ischemia reperfusion; drug delivery
MeSH headings : Acrylic Resins / chemical synthesis; Acrylic Resins / chemistry; Acrylic Resins / therapeutic use; Animals; Fibrosis / complications; Fibrosis / drug therapy; Fibrosis / metabolism; Ischemia / complications; Ischemia / drug therapy; Ischemia / metabolism; Myocardial Infarction / drug therapy; Myocardial Infarction / metabolism; Myocardial Infarction / pathology; Nanogels; Polyethylene Glycols / chemical synthesis; Polyethylene Glycols / chemistry; Polyethylene Glycols / therapeutic use; Polyethyleneimine / chemical synthesis; Polyethyleneimine / chemistry; Polyethyleneimine / therapeutic use; Rats
TL;DR: In vivo and in vitro, FSNs localize to fibrin in injured heart tissue and, when loaded with tPA and Y-27632, showed significant improvement in left ventricular ejection fraction 2 and 4 weeks post-I/R as well as significantly decreased infarct size, α-smooth muscle actin expression, and connective tissue growth factor expression 4 weeks after the I/R. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Crossref, NC State University Libraries
Added: February 21, 2020

2018 journal article

Targeted repair of heart injury by stem cells fused with platelet nanovesicles

Nature Biomedical Engineering, 2(1), 17–26.

By: J. Tang n, T. Su n, K. Huang n, P. Dinh n, Z. Wang*, A. Vandergriff n, M. Hensley n, J. Cores n ...

TL;DR: The attachment of platelet nanovesicles to the surface of cardiac stem cells increases the retention of the cells delivered to the heart and reduces infarct size in rat and pig models of acute myocardial infarction. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries, Crossref, ORCID
Added: August 6, 2018

2018 journal article

Viscoelastic properties of microgel thin films control fibroblast modes of migration and pro-fibrotic responses

Biomaterials, 185, 371–382.

By: D. Chester n, R. Kathard n, J. Nortey n, K. Nellenbach n & A. Brown n

author keywords: Microgels; Colloidal thin film; Fibroblasts; Mechanotransduction; Self-healing
MeSH headings : Biocompatible Materials / chemistry; Cell Adhesion; Cell Differentiation; Cell Line; Cell Movement; Elastic Modulus; Fibroblasts / cytology; Fibrosis / etiology; Gels / chemistry; Humans; Mechanotransduction, Cellular; Viscosity
TL;DR: Using microgel thin films with controllable non-linear elastic properties for investigating the role of material loss tangent on cell adhesion, migration, and myofibroblastic differentiation indicates that slight changes in tissue viscoelasticity following injury could contribute to early initiation of fibrotic related responses. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref, NC State University Libraries
Added: November 12, 2018

2017 journal article

Characterizing Cell Migration Within Three-dimensional <em>In Vitro</em> Wound Environments

Journal of Visualized Experiments, 8(126).

By: S. Nandi n & A. Brown n

author keywords: Bioengineering; Issue 126; cell migration; three dimensional; fibrin; wound healing; extracellular matrix; integrins; in vitro wound healing assay
MeSH headings : Bioengineering / methods; Cell Movement / physiology; Humans; Wound Healing / physiology
TL;DR: The method described herein allows for the study of cell migration in a three-dimensional model that better reflects physiological conditions than previously established two-dimensional scratch assays, thus increasing the relevance of in vitro cell studies prior to the use of in vivo animal models. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2017 journal article

Detection of an Integrin-Binding Mechanoswitch within Fibronectin during Tissue Formation and Fibrosis

ACS Nano, 11(7), 7110–7117.

By: L. Cao*, J. Nicosia*, J. Larouche*, Y. Zhang*, H. Bachman*, A. Brown n, L. Holmgren*, T. Barker*

author keywords: mechano-biology; antibody phage display; integrins; fibronectin; fibrosis; angiogenesis
MeSH headings : Animals; Biomechanical Phenomena; Cell Adhesion; Extracellular Matrix / metabolism; Fibronectins / analysis; Fibronectins / metabolism; Fibrosis; Integrins / analysis; Integrins / metabolism; Lung / metabolism; Lung / pathology; Mice; Neovascularization, Physiologic; Protein Binding
TL;DR: Direct experimental evidence is reported for the Fn integrin switch both in vitro and ex vivo using a scFv engineered to detect the transient, force-induced conformational change, representing an opportunity for detection and targeting of early molecular signatures of cell contractile forces in tissue repair and disease. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2017 journal article

Material Strategies for Modulating Epithelial to Mesenchymal Transitions

ACS Biomaterials Science & Engineering, 4(4), 1149–1161.

By: E. Mihalko n & A. Brown n

author keywords: epithelial to mesenchymal transition; EMT; mechanotransduction; fibrosis; development; transforming growth factor beta; EndoMT
TL;DR: Drivers of the similar process of endothelial to mesenchymal transition (EndoMT) are discussed and corresponding material strategies for controlling EndoMT are discussed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018

2017 journal article

Peptide Mimetic Drugs for Modulating Thrombosis and Hemostasis

Drug Development Research, 78(6), 236–244.

By: K. Nellenbach n & A. Brown n

author keywords: thrombosis; hemostasis; peptide-mimetic; fibrinogen; thrombin; platelet
MeSH headings : Animals; Blood Coagulation; Fibrinolytic Agents / pharmacology; Hemostasis / drug effects; Humans; Peptidomimetics / pharmacology; Risk Factors; Thrombosis / drug therapy
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018

2017 journal article

The role of biophysical properties of provisional matrix proteins in wound repair

Matrix Biology, 60-61, 124–140.

By: D. Chester n & A. Brown n

author keywords: Wound healing; Extracellular matrix; Biophysics; Fibrin; Fibrinogen; Fibronectin; Collagen; Transforming growth factor beta; Platelets
MeSH headings : Animals; Blood Platelets / cytology; Blood Platelets / drug effects; Blood Platelets / metabolism; Cell Communication / drug effects; Cell Proliferation / drug effects; Collagen / chemistry; Collagen / genetics; Collagen / metabolism; Dermis / injuries; Dermis / metabolism; Dermis / pathology; Epithelial Cells / cytology; Epithelial Cells / drug effects; Epithelial Cells / metabolism; Extracellular Matrix / drug effects; Extracellular Matrix / metabolism; Extracellular Matrix / ultrastructure; Fibroblasts / cytology; Fibroblasts / drug effects; Fibroblasts / metabolism; Fibronectins / chemistry; Fibronectins / genetics; Fibronectins / metabolism; Gene Expression; Humans; Integrins / chemistry; Integrins / genetics; Integrins / metabolism; Intercellular Signaling Peptides and Proteins / genetics; Intercellular Signaling Peptides and Proteins / metabolism; Intercellular Signaling Peptides and Proteins / pharmacology; Keratinocytes / cytology; Keratinocytes / drug effects; Keratinocytes / metabolism; Protein Subunits / chemistry; Protein Subunits / genetics; Protein Subunits / metabolism; Wound Healing / drug effects; Wound Healing / physiology
TL;DR: A review provides an overview of the ECM's role in the wound healing process and subsequently expands on the variety of roles biophysical phenomenon play. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2016 journal article

Fibrin Network Changes in Neonates after Cardiopulmonary Bypass

Anesthesiology, 124(5), 1021–1031.

By: A. Brown n, R. Hannan n, L. Timmins n, J. Fernandez n, T. Barker n & N. Guzzetta n

MeSH headings : Adult; Blood Coagulation; Blood Loss, Surgical; Blood Transfusion; Cardiopulmonary Bypass / adverse effects; Factor XIII / analysis; Female; Fibrin; Fibrinogen / metabolism; Humans; Infant; Infant, Newborn; Male; Microscopy, Confocal; Prothrombin / analysis
TL;DR: It is confirmed that significant differences exist in clot structure between neonates and adults and that neonatal and adult fibrinogen may not integrate well and suggested that differential treatment strategies for neonates should be pursued to reduce the demonstrated morbidity of blood product transfusion. (via Semantic Scholar)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018

2016 journal article

Platelet-mimetic strategies for modulating the wound environment and inflammatory responses

Experimental Biology and Medicine, 241(10), 1138–1148.

By: S. Nandi n & A. Brown n

author keywords: Platelets; hemostasis; artificial platelets; platelet-mimetic; nanoparticles; bionanoscience
MeSH headings : Artificial Cells; Blood Platelets / physiology; Inflammation / pathology; Inflammation / physiopathology; Models, Theoretical; Wounds and Injuries / pathology; Wounds and Injuries / physiopathology
TL;DR: Lyophilized platelets that can be freeze-dried and stored for longer periods of time and synthetic platelet-mimetic technologies that can enhance or replace the functions of natural platelets, while minimizing adverse immune responses have been explored as alternatives to transfusion. (via Semantic Scholar)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018

2016 journal article

Single-platelet nanomechanics measured by high-throughput cytometry

Nature Materials, 16(2), 230–235.

By: D. Myers*, Y. Qiu*, M. Fay*, M. Tennenbaum*, D. Chester n, J. Cuadrado*, Y. Sakurai*, J. Baek* ...

MeSH headings : Blood Coagulation / physiology; Blood Flow Velocity / physiology; Blood Platelets / physiology; Cells, Cultured; Elastic Modulus / physiology; Flow Cytometry / methods; Hardness / physiology; Humans; Mechanotransduction, Cellular / physiology; Nanoparticles / chemistry; Platelet Activation / physiology; Platelet Adhesiveness / physiology
TL;DR: A high-throughput hydrogel based platelet-contraction cytometer is reported that quantifies single-platelet contraction forces in different clot microenvironments and shows that platelets, via the Rho/ROCK pathway, synergistically couple mechanical and biochemical inputs to mediate contraction. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2015 journal article

Chondroitin Sulfate Glycosaminoglycan Hydrogels Create Endogenous Niches for Neural Stem Cells

BIOCONJUGATE CHEMISTRY, 26(12), 2336–2349.

By: L. Karumbaiah*, S. Enam, A. Brown n, T. Saxena, M. Betancur*, T. Barker, R. Bellamkonda

MeSH headings : Animals; Brain-Derived Neurotrophic Factor / administration & dosage; Cell Proliferation; Cells, Cultured; Chondroitin Sulfates / chemistry; Fibroblast Growth Factor 2 / administration & dosage; Hydrogels / chemistry; Neural Stem Cells / cytology; Rats; Tissue Scaffolds / chemistry
TL;DR: The ability of CS-GAG based hydrogels to regulate NSC self-renewal, and facilitate growth factor enrichment locally is demonstrated. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

Integrin α3β1 Binding to Fibronectin Is Dependent on the Ninth Type III Repeat

Journal of Biological Chemistry, 290(42), 25534–25547.

By: A. Brown n, M. Dysart*, K. Clarke*, S. Stabenfeldt* & T. Barker*

MeSH headings : Amino Acid Sequence; Cell Adhesion; Cell Line; Fibronectins / chemistry; Fibronectins / genetics; Fibronectins / metabolism; Humans; Integrin alpha3beta1 / metabolism; Molecular Sequence Data; Mutation; Protein Binding
TL;DR: These studies suggest that the presence and spacing of the RGD and synergy sites modulate integrin α3β1 binding to Fn. (via Semantic Scholar)
Sources: NC State University Libraries, NC State University Libraries, Crossref
Added: August 6, 2018

2015 journal article

Molecular interference of fibrin's divalent polymerization mechanism enables modulation of multiscale material properties

Biomaterials, 49, 27–36.

By: A. Brown*, S. Baker*, A. Douglas*, M. Keating*, M. Alvarez-Elizondo*, E. Botvinick*, M. Guthold*, T. Barker*

author keywords: Fibrin; Fibrinogen; Mechanical properties; Biocompatibility
MeSH headings : Biocompatible Materials / chemistry; Blood Coagulation; Fibrin / chemistry; Humans; Kinetics; Materials Testing; Microscopy, Confocal; Peptides / chemistry; Polyethylene Glycols / chemistry; Polymerization; Rheology; Stress, Mechanical; Surface Plasmon Resonance
TL;DR: The data strongly suggests that hole b engagement increases in single fiber stiffness that translates to higher storage moduli of fibrin polymers despite their increased porosity, and point to possible strategies for tuning fibr in polymer mechanical properties through modulation of single fiber mechanics. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 16, 2021

2015 journal article

Ultrasoft, highly deformable microgels

Soft Matter, 11(10), 2018–2028.

By: H. Bachman*, A. Brown*, K. Clarke*, K. Dhada*, A. Douglas*, C. Hansen*, E. Herman*, J. Hyatt* ...

MeSH headings : Acrylamides; Acrylates / chemistry; Acrylic Resins / chemistry; Ammonium Sulfate / chemistry; Cross-Linking Reagents / chemistry; Hydrogels / chemistry; Isocyanates / chemistry; Polyethylene Glycols / chemistry; Rheology; Silanes / chemistry; Sodium Dodecyl Sulfate / chemistry
TL;DR: Due to their softness and deformability, ULC microgels are a unique base material for a wide variety of biomedical applications including biomaterials for drug delivery and regenerative medicine. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 16, 2021

2014 journal article

Direct observation of ligand-induced receptor dimerization with a bioresponsive hydrogel

RSC Advances, 4(110), 65173–65175.

By: J. Kim*, Y. Park*, A. Brown* & L. Lyon*

TL;DR: A simple yet versatile method for the identification of physiologically important receptor dimerization events induced by a ligand is demonstrated and it is identified that one thrombin molecule associates with two GPIbα moieties, which may play a role in efficient hemostatic function by increasing local concentration of G PIbα on platelet surfaces. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: October 23, 2021

2014 journal article

Fibrin Fibers: Blocking the B:B Knob-Pocket Interaction

Biophysical Journal, 106(2), 614a.

By: S. Baker*, A. Carson-Brown*, M. Guthold* & T. Barker*

TL;DR: It is found that the density and lateral aggregation of fibers was hindered by blocking the b-pockets with the synthetic B-knobs with the fibrinogen solution. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: October 23, 2021

2014 journal article

Fibrin-based biomaterials: Modulation of macroscopic properties through rational design at the molecular level

Acta Biomaterialia, 10(4), 1502–1514.

By: A. Brown* & T. Barker*

author keywords: Fibrin; Biomaterial design; Fibrin glue; Wound repair; Tissue engineering
MeSH headings : Animals; Biocompatible Materials / chemistry; Biocompatible Materials / pharmacology; Fibrin / chemistry; Fibrin / pharmacology; Humans; Polymerization / drug effects; Proteolysis / drug effects; Tissue Engineering / methods
TL;DR: Modifying the macroscopic properties of fibrin, such as elasticity and porosity, has been somewhat elusive until recently, yet with a molecular-level rational design approach it can be somewhat easily modified through alterations of molecular interactions key to the protein's polymerization process. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 16, 2021

2014 journal article

Learning from nature – Novel synthetic biology approaches for biomaterial design

Acta Biomaterialia, 10(4), 1761–1769.

By: A. Bryksin*, A. Brown*, M. Baksh*, M. Finn* & T. Barker*

author keywords: Synthetic biology; Biomaterials; Directed evolution; Tissue engineering; Virus particles
MeSH headings : Biocompatible Materials / chemistry; Polymers / chemistry; Synthetic Biology / methods; Tissue Scaffolds / chemistry; Virion / chemistry
TL;DR: The purpose of this review is to highlight the most recent advances in the use of molecular evolution in synthetic biology applications for biomaterial engineering, and to discuss some of the areas in which this approach may be successfully applied in the future. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: October 23, 2021

2014 journal article

Platelet mechanosensing of substrate stiffness during clot formation mediates adhesion, spreading, and activation

Proceedings of the National Academy of Sciences, 111(40), 14430–14435.

By: Y. Qiu*, A. Brown*, D. Myers*, Y. Sakurai*, R. Mannino*, R. Tran*, B. Ahn*, E. Hardy* ...

author keywords: mechanotransduction; cell mechanics; platelet cytoskeleton; biomaterials
MeSH headings : Acrylic Resins / metabolism; Blood Coagulation / physiology; Blood Platelets / cytology; Blood Platelets / metabolism; Cell Movement / physiology; Cellular Microenvironment / physiology; Fibrin / metabolism; Fibrinogen / metabolism; Humans; Immobilized Proteins / metabolism; Mechanotransduction, Cellular / physiology; Microscopy, Confocal; P-Selectin / metabolism; Phosphatidylserines / metabolism; Platelet Activation / physiology; Platelet Adhesiveness / physiology; Platelet Aggregation / physiology; Platelet Glycoprotein GPIIb-IIIa Complex / metabolism; Stress, Mechanical; Thrombosis / physiopathology; rac1 GTP-Binding Protein / metabolism
TL;DR: It is shown for the first time that platelets sense the stiffness of the underlying clot substrate, and increasing substrate stiffness increases platelet adhesion and spreading, and provides biophysical insight into the underlying mechanisms of platelet aggregation and platelet activation heterogeneity during thrombus formation. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: October 23, 2021

2014 journal article

Ultrasoft microgels displaying emergent platelet-like behaviours

Nature Materials, 13(12), 1108–1114.

By: A. Brown*, S. Stabenfeldt*, B. Ahn*, R. Hannan*, K. Dhada*, E. Herman*, V. Stefanelli*, N. Guzzetta* ...

TL;DR: In vitro and in silico analyses demonstrate that PLPs actively collapse fibrin networks, an emergent behaviour that mimics in vivo clot contraction and should inform the future design of a broader class of dynamic, biosynthetic composite materials. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: October 23, 2021

2013 journal article

Colloid-matrix assemblies in regenerative medicine

Current Opinion in Colloid & Interface Science, 18(5), 393–405.

By: K. Clarke, A. Douglas, A. Brown*, T. Barker & L. Lyon

author keywords: Extracellular matrix; Fibrin; Colloids; Composites; Tissue engineering; Regenerative medicine
TL;DR: This review describes a variety of strategies aimed at reproducing and also simplifying the ECM, and focuses on colloidal particles as potential artificial ECM components that could increase the complexity as modular building blocks. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: October 23, 2021

2013 journal article

Physical and chemical microenvironmental cues orthogonally control the degree and duration of fibrosis-associated epithelial-to-mesenchymal transitions

The Journal of Pathology, 229(1), 25–35.

By: A. Brown*, V. Fiore*, T. Sulchek* & T. Barker*

author keywords: idiopathic pulmonary fibrosis; epithelial-to-mesenchymal transition; fibrosis; stiffness; cell contractility; alveolar epithelial cell
MeSH headings : Alveolar Epithelial Cells / metabolism; Alveolar Epithelial Cells / pathology; Animals; Biomechanical Phenomena; Bleomycin; Cells, Cultured; Cellular Microenvironment; Coculture Techniques; Disease Models, Animal; Elastic Modulus; Epithelial-Mesenchymal Transition; Fibronectins / metabolism; Integrins / metabolism; Laminin / metabolism; Lung / metabolism; Lung / pathology; Male; Mechanotransduction, Cellular; Mice; Mice, Inbred C57BL; Microscopy, Atomic Force; Mink; Nanotechnology; Phenotype; Pulmonary Fibrosis / metabolism; Pulmonary Fibrosis / pathology; Rats; Rats, Sprague-Dawley; Thrombin / metabolism; Time Factors; Transforming Growth Factor beta / metabolism
TL;DR: In vitro research finds that stiff, but not soft, fibronectin substrates induce EMT, a response dependent on cell contraction‐mediated integrin activation of TGFβ. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: October 23, 2021

2013 journal article

The Mechanical Properties of Modified Fibrin Fibers: Blocking the B-b Knob-Pocket Interaction

Biophysical Journal, 104(2), 512a–513a.

By: S. Baker*, A. Carson Brown, T. Barker* & M. Guthold*

TL;DR: Fibrin clot formation was studied to determine the mechanical properties of fibrin fibers modified by blocking the B-b knob-pocket interaction and it was found that the density and lateral aggregation of fibers was hindered byblocking the b-pockets with the synthetic B-knobs. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: October 23, 2021

2012 journal article

Directing epithelial to mesenchymal transition through engineered microenvironments displaying orthogonal adhesive and mechanical cues

Journal of Biomedical Materials Research Part A, 100A(8), 2119–2127.

By: M. Markowski*, A. Brown* & T. Barker*

author keywords: epithelial to mesenchymal transition; fibronectin mutant; substrate stiffness; cell contractility; alveolar epithelial cell
MeSH headings : Animals; Biomarkers / metabolism; Cell Adhesion / drug effects; Cell Line; Cellular Microenvironment / drug effects; Cues; Epithelial Cells / cytology; Epithelial-Mesenchymal Transition / drug effects; Fibronectins / chemistry; Fibronectins / pharmacology; Mink; Phenotype; Protein Stability / drug effects; Pulmonary Alveoli / cytology; Repetitive Sequences, Amino Acid; Transforming Growth Factor beta / pharmacology
TL;DR: C cultured lung epithelial cells on combinations of different mutants of fibronectin's cell binding domain that preferentially engage specific integrins and substrates of varying stiffness suggest that integrin-specific engagement of fibRONectin adhesive domains and the mechanics of the ECM act synergistically to direct EMT. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: October 23, 2021

2011 journal article

Guiding Epithelial Cell Phenotypes with Engineered Integrin-Specific Recombinant Fibronectin Fragments

Tissue Engineering Part A, 17(1-2), 139–150.

By: A. Brown*, J. Rowe* & T. Barker*

Sources: Crossref, NC State University Libraries
Added: May 17, 2024

2010 journal article

Continuously perfused, non-cross-contaminating microfluidic chamber array for studying cellular responses to orthogonal combinations of matrix and soluble signals

Lab Chip, 10(5), 571–580.

By: E. Park*, A. Brown*, M. DiFeo*, T. Barker* & H. Lu*

MeSH headings : Cell Culture Techniques / instrumentation; Cells, Cultured; Epithelial Cells / drug effects; Epithelial Cells / physiology; Equipment Design; Equipment Failure Analysis; Extracellular Matrix Proteins / metabolism; Flow Injection Analysis / instrumentation; Humans; Microfluidic Analytical Techniques / instrumentation; Reproducibility of Results; Sensitivity and Specificity; Solubility; Specimen Handling / instrumentation; Transforming Growth Factor beta1 / pharmacology
TL;DR: The device is used to characterize different phenotypes of alveolar epithelial type II (ATII) cells, particularly the extent of epithelial-to-mesenchymal transition (EMT), a highly suspected pathway in tissue regeneration and fibrosis. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: October 23, 2021

2009 journal article

Emerging concepts in engineering extracellular matrix variants for directing cell phenotype

Regenerative Medicine, 4(4), 593–600.

By: A. Carson* & T. Barker*

Sources: Crossref, NC State University Libraries
Added: May 17, 2024

Funding History

Funding history based on the linked ORCID record. Updated: December 12th, 2023 10:34

grant July 1, 2023 - June 30, 2026
Dynamic injectable scaffolds to enhance therapeutic potential of satellite cells for peripheral artery disease
American Heart Association
grant July 1, 2022 - June 30, 2025
Leveraging Age-Dependent Clotting Mechanisms for Hemostatic Material Design
American Heart Association

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