@article{laney_acosta-leal_rotenberg_2018, title={Optimized Yellow Dwarf Virus Multiplex PCR Assay Reveals a Common Occurrence of Barley yellow dwarf virus-PAS in Kansas Winter Wheat}, volume={19}, ISSN={["1535-1025"]}, DOI={10.1094/PHP-09-17-0056-RS}, abstractNote={ Barley yellow dwarf is an aphid-transmitted virus disease caused by yellow dwarf virus (YDV) species in the family Luteoviridae. Previous partial sequencing efforts conducted in Kansas revealed that Barley yellow dwarf virus-PAS (PAS) occurs in winter wheat fields, and currently available YDV multiplex reverse-transcription PCR (RT-PCR) assays do not detect this species. To enable precise determination of YDV species for research, disease diagnostic, and plant breeding programs, this study enhanced and validated the utility of a multiplex RT-PCR protocol to discriminate six YDV species, including PAS, in archived and fresh field samples. From a representative subset of samples collected from commercial and variety trial locations across nine wheat growing regions of Kansas, PAS and Barley yellow dwarf virus-PAV (PAV) were equally prevalent in single or mixed infections, and other YDVs occurred in mixed infections with PAS and/or PAV in low numbers. The optimized multiplex assay provided robust and specific detection of YDVs and showed promise as a diagnostic tool for determining species occurrence and composition of YDVs in an intensive wheat cropping region of the United States. }, number={1}, journal={PLANT HEALTH PROGRESS}, author={Laney, Alma G. and Acosta-Leal, Rodolfo and Rotenberg, Dorith}, year={2018}, pages={37–43} } @article{laney_chen_korth_2018, title={Interactive effects of aphid feeding and virus infection on host gene expression and volatile compounds in salt-stressed soybean, Glycine max (L.) Merr}, volume={12}, ISSN={["1872-8847"]}, DOI={10.1007/s11829-017-9590-9}, number={3}, journal={ARTHROPOD-PLANT INTERACTIONS}, author={Laney, Alma G. and Chen, Pengyin and Korth, Kenneth L.}, year={2018}, month={Jun}, pages={401–413} }