@article{hodgens_flaherty_pullen_khan_english_gillan_rojas-pierce_akpa_2024, title={Model-based inference of a dual role for HOPS in regulating guard cell vacuole fusion}, volume={6}, ISSN={["2517-5025"]}, url={http://dx.doi.org/10.1093/insilicoplants/diae015}, DOI={10.1093/insilicoplants/diae015}, abstractNote={Abstract Guard cell movements depend, in part, on the remodelling of vacuoles from a highly fragmented state to a fused morphology during stomata opening. Indeed, full opening of plant stomata requires vacuole fusion to occur. Fusion of vacuole membranes is a highly conserved process in eukaryotes, with key roles played by two multi-subunit complexes: HOPS (homotypic fusion and vacuolar protein sorting) and SNARE (soluble NSF attachment protein receptor). HOPS is a vacuole tethering factor that is thought to chaperone SNAREs from apposing vacuole membranes into a fusion-competent complex capable of rearranging membranes. In plants, recruitment of HOPS subunits to the tonoplast has been shown to require the presence of the phosphoinositide phosphatidylinositol 3-phosphate. However, chemically depleting this lipid induces vacuole fusion. To resolve this counter-intuitive observation regarding the role of HOPS in regulating plant vacuole morphology, we defined a quantitative model of vacuole fusion dynamics and used it to generate testable predictions about HOPS-SNARE interactions. We derived our model by using simulation-based inference to integrate prior knowledge about molecular interactions with limited, qualitative observations of emergent vacuole phenotypes. By constraining the model parameters to yield the emergent outcomes observed for stoma opening—as induced by two distinct chemical treatments—we predicted a dual role for HOPS and identified a stalled form of the SNARE complex that differs from phenomena reported in yeast. We predict that HOPS has contradictory actions at different points in the fusion signalling pathway, promoting the formation of SNARE complexes, but limiting their activity.}, number={2}, journal={IN SILICO PLANTS}, author={Hodgens, Charles and Flaherty, D. T. and Pullen, Anne-Marie and Khan, Imran and English, Nolan J. and Gillan, Lydia and Rojas-Pierce, Marcela and Akpa, Belinda S.}, editor={Zhu, Xin-GuangEditor}, year={2024}, month={Nov} }
 @article{defalco_anne_james_willoughby_schwanke_johanndrees_genolet_derbyshire_wang_rana_et al._2022, title={A conserved module regulates receptor kinase signalling in immunity and development}, volume={8}, url={http://dx.doi.org/10.1038/s41477-022-01134-w}, DOI={10.1038/s41477-022-01134-w}, number={4}, journal={Nature Plants}, publisher={Springer Science and Business Media LLC}, author={DeFalco, Thomas A. and Anne, Pauline and James, Sean R. and Willoughby, AC and Schwanke, Florian and Johanndrees, Oliver and Genolet, Yasmine and Derbyshire, Paul and Wang, Qian and Rana, Surbhi and et al.}, year={2022}, month={Apr}, pages={356–365} }
 @phdthesis{noblitt_2019, title={Regulating Stem Cell Populations Through Phosphorylation of POLTERGEIST in Arabidopsis}, url={https://cdr.lib.unc.edu/concern/honors_theses/ms35tf001}, DOI={10.17615/A0WQ-5665}, journal={The University of North Carolina at Chapel Hill University Libraries}, school={The University of North Carolina at Chapel Hill University Libraries}, author={Noblitt, Anne-Marie}, year={2019} }