Works (24)

Updated: August 4th, 2025 07:28

2025 article

Spiner, deep learning-based automated detection of spiral ganglion neurons in intact cochleae

Cai, Y., Leitz-Najarian, G. M., Rosen, R., Hutson, K. A., Moatti, A., Li, C., … Greenbaum, A. (2025, June 18). IScience.

By: Y. Cai n, G. Leitz-Najarian*, R. Rosen, K. Hutson*, A. Moatti n, C. Li n, D. Fitzpatrick*, A. Greenbaum n

topics (OpenAlex): Hearing Loss and Rehabilitation; Hearing, Cochlea, Tinnitus, Genetics; Structural Health Monitoring Techniques
Source: Web Of Science
Added: July 28, 2025

2024 article

A gene edited pig model for studying LGR5+ stem cells: implications for future applications in tissue regeneration and biomedical research

Hill, A. B. T., Murphy, Y. M., Polkoff, K. M., Edwards, L., Walker, D. M., Moatti, A., … Piedrahita, J. A. (2024, June 6). Frontiers in Genome Editing, Vol. 6.

By: A. Hill n, Y. Murphy n, K. Polkoff n, L. Edwards n, D. Walker n, A. Moatti n, A. Greenbaum n, J. Piedrahita n

author keywords: LGR5; gene edited pigs; organ stem cells; regenerative medicine; animal models
topics (OpenAlex): Pluripotent Stem Cells Research; Cancer Cells and Metastasis; Neonatal Respiratory Health Research
Sources: Web Of Science, NC State University Libraries
Added: July 1, 2024

2024 article

Intelligent Beam Optimization for Light-Sheet Fluorescence Microscopy through Deep Learning

Li, C., Rai, M. R., Cai, Y., Ghashghaei, H. T., & Greenbaum, A. (2024, January 1). Intelligent Computing.

By: C. Li n, M. Rai n, Y. Cai n, H. Ghashghaei n & A. Greenbaum*

topics (OpenAlex): Advanced Fluorescence Microscopy Techniques; Image Processing Techniques and Applications; Optical Coherence Tomography Applications
Source: Web Of Science
Added: January 13, 2025

2024 article

Novel Porcine Model Reveals Two Distinct LGR5 Cell Types during Lung Development and Homeostasis

Polkoff, K. M., Lampe, R., Gupta, N. K., Murphy, Y., Chung, J., Carter, A., … Piedrahita, J. A. (2024, November 5). American Journal of Respiratory Cell and Molecular Biology, Vol. 72, pp. 496–509.

By: K. Polkoff n, R. Lampe n, N. Gupta n, Y. Murphy n, J. Chung n, A. Carter n, J. Simon*, K. Gleason n ...

author keywords: pig model; LGR5; lung development; nerve fibroblasts
MeSH headings : Animals; Receptors, G-Protein-Coupled / metabolism; Receptors, G-Protein-Coupled / genetics; Lung / metabolism; Lung / embryology; Lung / cytology; Lung / growth & development; Homeostasis; Swine; Humans; Mice; Organoids / metabolism; Fibroblasts / metabolism; Epithelial Cells / metabolism; Models, Animal; Animals, Genetically Modified
topics (OpenAlex): Neonatal Respiratory Health Research; Congenital Diaphragmatic Hernia Studies; Respiratory Support and Mechanisms
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: May 19, 2025

2024 article

topics (OpenAlex): Digital Holography and Microscopy; Random lasers and scattering media; Advanced Optical Imaging Technologies
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4. Quality Education (OpenAlex)
Source: Web Of Science
Added: September 16, 2024

2024 journal article

Surgical procedure of intratympanic injection and inner ear pharmacokinetics simulation in domestic pigs

Frontiers in Pharmacology, 15.

By: A. Moatti n, S. Connard n, N. De Britto n, W. Dunn*, S. Rastogi n, M. Rai n, L. Schnabel n, F. Ligler* ...

author keywords: intratympanic; inner ear; fluid simulation; round window membrane; pigs; swine; pharmacokinetics
topics (OpenAlex): Vestibular and auditory disorders; Hearing, Cochlea, Tinnitus, Genetics; Ear Surgery and Otitis Media
TL;DR: The potential of using pigs as a large animal model for studying inner ear pharmacokinetic studies of the inner ear using pigs is demonstrated and the diffusion time and drug concentrations reported align closely with the limited data available from human studies. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: February 19, 2024

2023 article

Assessment of drug permeability through an ex vivo porcine round window membrane model

Moatti, A., Silkstone, D., Martin, T., Abbey, K., Hutson, K. A., Fitzpatrick, D. C., … Greenbaum, A. (2023, May 2). IScience.

By: A. Moatti n, D. Silkstone n, T. Martin n, K. Abbey n, K. Hutson*, D. Fitzpatrick*, C. Zdanski*, A. Cheng*, F. Ligler*, A. Greenbaum n

topics (OpenAlex): Vestibular and auditory disorders; Hearing, Cochlea, Tinnitus, Genetics; Hearing Loss and Rehabilitation
TL;DR: An ex vivo porcine RWM model, similar in structure and thickness to the human RWM, is developed, which provides a straightforward approach to developing effective and non-invasive delivery methods to the inner ear. (via Semantic Scholar)
Source: Web Of Science
Added: June 26, 2023

2023 journal article

Bulk and mosaic deletions of Egfr reveal regionally defined gliogenesis in the developing mouse forebrain

IScience, 26(3), 106242.

By: X. Zhang n, G. Xiao n, C. Johnson n, Y. Cai n, Z. Horowitz n, C. Mennicke n, R. Coffey*, M. Haider* ...

topics (OpenAlex): MicroRNA in disease regulation; Neurogenesis and neuroplasticity mechanisms; RNA Research and Splicing
TL;DR: Subtractive bulk ventral and dorsal forebrain deletions of Egfr uncovered significant and permanent decreases in oligodendrogenesis and myelination in the cortex and corpus callosum, and a regenerative population of gliogenic progenitors in the mouse forebrain may compensate for the missing EGFR-dependent dorsal glia in the bulk Egfr-deleted forebrain. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: June 5, 2023

2023 article

COMBINe enables automated detection and classification of neurons and astrocytes in tissue-cleared mouse brains

Cai, Y., Zhang, X., Li, C., Ghashghaei, H. T., & Greenbaum, A. (2023, April 1). Cell Reports Methods.

By: Y. Cai n, X. Zhang n, C. Li n, H. Ghashghaei n & A. Greenbaum n

MeSH headings : Animals; Mice; Astrocytes; Excipients; Microscopy, Fluorescence; Neurons; Prosencephalon
topics (OpenAlex): Cell Image Analysis Techniques; Single-cell and spatial transcriptomics; Advanced Fluorescence Microscopy Techniques
TL;DR: An automated workflow to map sparsely labeled neurons and astrocytes in genetically distinct mouse forebrains using mosaic analysis with double markers (MADM) is presented and the regional and subregional effects of MADM-based deletion of the epidermal growth factor receptor (EGFR) are quantitatively analyzed. (via Semantic Scholar)
Source: Web Of Science
Added: May 30, 2023

2023 article

Deep learning-based adaptive optics for light sheet fluorescence microscopy

Rai, M. R., Li, C., Ghashghaei, H. T., & Greenbaum, A. (2023, May 19). Biomedical Optics Express.

By: M. Rai n, C. Li n, H. Ghashghaei n & A. Greenbaum n

topics (OpenAlex): Advanced Fluorescence Microscopy Techniques; Optical Coherence Tomography Applications; Photoacoustic and Ultrasonic Imaging
TL;DR: It is shown that the application of correction using a deformable mirror greatly improves image quality and two conceptually different network architectures are compared; one that shares convolutional features and another that estimates each aberration independently. (via Semantic Scholar)
Source: Web Of Science
Added: July 24, 2023

2023 article

Specific Inhibition of Orai1-mediated Calcium Signalling Resolves Inflammation and Clears Bacteria in an Acute Respiratory Distress Syndrome Model

Ahmad, S., Wrennall, J. A., Goriounova, A. S., Sekhri, M., Iskarpatyoti, J. A., Ghosh, A., … Tarran, R. (2023, November 16). American Journal of Respiratory and Critical Care Medicine.

By: S. Ahmad*, J. Wrennall*, A. Goriounova*, M. Sekhri*, J. Iskarpatyoti*, A. Ghosh*, S. Abdelwahab*, A. Voeller* ...

author keywords: peptide; ICRAC; ARDS; pneumonia; neutrophilia
MeSH headings : Humans; Mice; Animals; Calcium Channels / metabolism; Calcium Channels / pharmacology; Calcium / metabolism; HEK293 Cells; Methicillin-Resistant Staphylococcus aureus / metabolism; Calcium Signaling; Inflammation / drug therapy; Lung / metabolism; Respiratory Distress Syndrome / drug therapy; Pneumonia, Bacterial / drug therapy; ORAI1 Protein / metabolism; ORAI1 Protein / pharmacology
topics (OpenAlex): Ion Channels and Receptors; Respiratory and Cough-Related Research; Asthma and respiratory diseases
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UN Sustainable Development Goal Categories
Source: Web Of Science
Added: May 20, 2024

2023 article

Tissue clearing and three-dimensional imaging of the whole cochlea and vestibular system from multiple large-animal models

Moatti, A., Cai, Y., Li, C., Popowski, K. D., Cheng, K., Ligler, F. S., & Greenbaum, A. (2023, April 14). STAR Protocols.

By: A. Moatti n, Y. Cai n, C. Li n, K. Popowski n, K. Cheng n, F. Ligler*, A. Greenbaum n

author keywords: imaged cochleae using open -source software.; several mm; larger animal inner; open -source software
topics (OpenAlex): Single-cell and spatial transcriptomics; Cell Image Analysis Techniques; Photoacoustic and Ultrasonic Imaging
TL;DR: A protocol that enables three-dimensional (3D) characterization of intact inner ears from large-animal models is presented and a pipeline to count hair cells in antibody-stained and 3D imaged cochleae using open-source software is detailed. (via Semantic Scholar)
Source: Web Of Science
Added: September 5, 2023

2022 article

Illumination angle correction during image acquisition in light-sheet fluorescence microscopy using deep learning

Li, C., Rai, M. R., Ghashghaei, H. T., & Greenbaum, A. (2022, January 6). Biomedical Optics Express.

By: C. Li n, M. Rai n, H. Ghashghaei n & A. Greenbaum n

topics (OpenAlex): Advanced Fluorescence Microscopy Techniques; Image Processing Techniques and Applications; Cell Image Analysis Techniques
TL;DR: This study provides a framework that can correct the angle of the light-sheet and improve the overall image quality in high-resolution LSFM 3D image acquisition. (via Semantic Scholar)
Source: Web Of Science
Added: February 14, 2022

2022 journal article

Inhalable dry powder mRNA vaccines based on extracellular vesicles

Matter, 5(9), 2960–2974.

By: K. Popowski n, A. Moatti n, G. Scull n, D. Silkstone n, H. Lutz n, B. López de Juan Abad n, A. George n, E. Belcher n ...

topics (OpenAlex): Extracellular vesicles in disease; RNA Interference and Gene Delivery; Inhalation and Respiratory Drug Delivery
TL;DR: The results suggest that extracellular vesicles can serve as an inhaled mRNA drug-delivery system that is superior to synthetic liposomes. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: October 17, 2022

2022 article

Maternal organophosphate flame retardant exposure alters the developing mesencephalic dopamine system in fetal rat

Newell, A. J., Kapps, V. A., Cai, Y., Rai, M. R., Armour, G. S., Horman, B. M., … Patisaul, H. B. (2022, December 22). Toxicological Sciences, Vol. 191, pp. 357–373.

By: A. Newell n, V. Kapps n, Y. Cai n, M. Rai n, G. Armour n, B. Horman n, K. Rock n, S. Witchey n, A. Greenbaum n, H. Patisaul n

author keywords: neurodevelopment; monoamine; OPFR; OPE; flame retardants; neurotransmitters; neurotoxicity; neurotoxicology; prenatal; reproductive and developmental toxicology; mesocortical dopamine system; tissue clearing light sheet microscopy.
MeSH headings : Animals; Female; Male; Rats; Dopamine / metabolism; Fetal Development / drug effects; Flame Retardants / toxicity; Maternal Exposure / adverse effects; Neurotoxicity Syndromes / etiology; Organophosphates / toxicity; Serotonin / metabolism
topics (OpenAlex): Toxic Organic Pollutants Impact; Effects and risks of endocrine disrupting chemicals; Carcinogens and Genotoxicity Assessment
TL;DR: Results indicate maternal exposure to OPFRs alters the development trajectory of the embryonic dopaminergic system and adds to growing evidence of OPFR developmental neurotoxicity. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: February 12, 2024

2022 article

Ontogeny of cellular organization and LGR5 expression in porcine cochlea revealed using tissue clearing and 3D imaging

Moatti, A., Li, C., Sivadanam, S., Cai, Y., Ranta, J., Piedrahita, J. A., … Greenbaum, A. (2022, June 30). IScience, Vol. 25.

By: A. Moatti n, C. Li n, S. Sivadanam n, Y. Cai n, J. Ranta n, J. Piedrahita n, A. Cheng*, F. Ligler n, A. Greenbaum n

topics (OpenAlex): Hearing, Cochlea, Tinnitus, Genetics; Molecular Biology Techniques and Applications; Bat Biology and Ecology Studies
TL;DR: Data reveal that hair cell organization, inner ear morphology, cellular cartography in the organ of Corti, and spatiotemporal expression of LGR5 are dynamic over developmental stages in a pattern not previously documented. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: December 19, 2022

2022 article

Quantitative analysis of illumination and detection corrections in adaptive light sheet fluorescence microscopy

Rai, M. R., Li, C., & Greenbaum, A. (2022, April 18). Biomedical Optics Express.

By: M. Rai n, C. Li n & A. Greenbaum n

topics (OpenAlex): Advanced Fluorescence Microscopy Techniques; Cell Image Analysis Techniques; Photoacoustic and Ultrasonic Imaging
Source: Web Of Science
Added: May 31, 2022

2021 article

Deep learning-based autofocus method enhances image quality in light-sheet fluorescence microscopy: publisher’s note

Li, C., Moatti, A., Zhang, X., Ghashghaei, H. T., & Greenbaum, A. (2021, December 8). Biomedical Optics Express.

By: C. Li n, A. Moatti n, X. Zhang n, H. Ghashghaei n & A. Greenbaum n

topics (OpenAlex): Advanced Fluorescence Microscopy Techniques; Cell Image Analysis Techniques; Image Processing Techniques and Applications
Source: Web Of Science
Added: January 10, 2022

2021 article

Detection and classification of neurons and glial cells in the MADM mouse brain using RetinaNet

Cai, Y., Zhang, X., Kovalsky, S. Z., Ghashghaei, H. T., & Greenbaum, A. (2021, September 24). PLoS ONE.

By: Y. Cai n, X. Zhang n, S. Kovalsky*, H. Ghashghaei n & A. Greenbaum n

MeSH headings : Animals; Brain; Cell Differentiation; Mice; Neuroglia; Neurons
topics (OpenAlex): Cell Image Analysis Techniques; Single-cell and spatial transcriptomics; Advanced Fluorescence Microscopy Techniques
TL;DR: Object detection networks (neural networks) are utilized to detect and classify targets in complex microscopy images, while simplifying data annotation, and merging the predictions of the two computational models significantly improved the automated cell counting of glial clusters. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
8. Decent Work and Economic Growth (OpenAlex)
Source: Web Of Science
Added: February 28, 2022

2021 article

Enhancement of Bone Regeneration Through the Converse Piezoelectric Effect, A Novel Approach for Applying Mechanical Stimulation

Carter, A., Popowski, K., Cheng, K., Greenbaum, A., Ligler, F. S., & Moatti, A. (2021, September 24). Bioelectricity, Vol. 9.

By: A. Carter n, K. Popowski n, K. Cheng n, A. Greenbaum n, F. Ligler n & A. Moatti n

author keywords: converse piezoelectric effect; mechanical stimulation; bone regeneration
topics (OpenAlex): Bone Tissue Engineering Materials; Planarian Biology and Electrostimulation
TL;DR: The potential of the converse piezoelectric effect to enhance bone regeneration is highlighted by discussing the activation of beneficial cellular pathways, the properties of piezOElectric biomaterials, and the potential for the more effective administration of the Converse pieZoelectrics effect using wireless control. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: September 25, 2021

2021 article

Light-guided sectioning for precise in situ localization and tissue interface analysis for brain-implanted optical fibers and GRIN lenses

Kahan, A., Greenbaum, A., Jang, M. J., Robinson, J. E., Cho, J. R., Chen, X., … Gradinaru, V. (2021, September 1). Cell Reports.

MeSH headings : Animals; Brain / physiology; Head / physiology; Lens, Crystalline / physiology; Lenses; Mice; Neurons / physiology; Optical Fibers; Photons; Refractometry / methods
topics (OpenAlex): Photoreceptor and optogenetics research; Neuroscience and Neural Engineering; Advanced Fluorescence Microscopy Techniques
TL;DR: A three-dimensional post hoc histology method called “light-guided sectioning” (LiGS), which preserves the tissue with its optical implant in place and allows staining and clearing of a volume up to 500 μm in depth, is developed. (via Semantic Scholar)
Source: Web Of Science
Added: October 26, 2021

2020 article

Multiplexed Cre-dependent selection yields systemic AAVs for targeting distinct brain cell types

Kumar, S. R., Miles, T. F., Chen, X., Brown, D., Dobreva, T., Huang, Q., … Gradinaru, V. (2020, April 20). Nature Methods.

By: S. Kumar*, T. Miles*, X. Chen*, D. Brown*, T. Dobreva*, Q. Huang*, X. Ding*, Y. Luo* ...

MeSH headings : Animals; Blood-Brain Barrier / metabolism; Brain / virology; Capsid Proteins / genetics; Capsid Proteins / metabolism; Dependovirus / genetics; Female; Gene Transfer Techniques; Genetic Engineering / methods; Genetic Therapy; Genetic Vectors / genetics; Integrases / metabolism; Male; Mice; Mice, Inbred C57BL; Organ Specificity; Viral Tropism
topics (OpenAlex): CRISPR and Genetic Engineering; Viral Infectious Diseases and Gene Expression in Insects; Virus-based gene therapy research
TL;DR: M-CREATE is an in vivo screening strategy for identifying recombinant AAVs with desired tropism that can cross the blood–brain barrier in adult mice across strains when delivered intravenously and accelerates the discovery of capsids for use in neuroscience and gene-therapy applications. (via Semantic Scholar)
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Source: Web Of Science
Added: May 8, 2020

2020 article

Phenotyping Intact Mouse Bones Using Bone CLARITY

Treweek, J. B., Beres, A., Johnson, N., & Greenbaum, A. (2020, November 16). Methods in Molecular Biology.

By: J. Treweek*, A. Beres n, N. Johnson n & A. Greenbaum n

author keywords: Tissue Clearing; Bone CLARITY; Three-dimensional imaging; Light-sheet microscopy; Fluorescence microscopy
MeSH headings : Animals; Bone and Bones / diagnostic imaging; Bone and Bones / physiology; Heme / chemistry; Humans; Hydrogels / pharmacology; Imaging, Three-Dimensional / methods; Mice; Optical Imaging / methods; Phenotype; Skull / diagnostic imaging; Skull / physiology; Staining and Labeling / methods
topics (OpenAlex): Cell Image Analysis Techniques; Molecular Biology Techniques and Applications; 3D Printing in Biomedical Research
TL;DR: A bone-clearing methodology, termed Bone CLARITY, which combines published techniques for clearing soft tissues, including delipidation for the removal of light-scattering membranes, hydrogel-embedding for the stabilization of fragile epitopes, and heme elution for the reduction of blood-based autofluorescence. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 9, 2021

2020 article

Three-dimensional imaging of intact porcine cochlea using tissue clearing and custom-built light-sheet microscopy

Moatti, A., Cai, Y., Li, C., Sattler, T., Edwards, L., Piedrahita, J., … Greenbaum, A. (2020, September 30). Biomedical Optics Express, Vol. 11, pp. 6181–6196.

By: A. Moatti n, Y. Cai n, C. Li n, T. Sattler n, L. Edwards n, J. Piedrahita n, F. Ligler n, A. Greenbaum n

topics (OpenAlex): Advanced Fluorescence Microscopy Techniques; Cell Image Analysis Techniques; Photoacoustic and Ultrasonic Imaging
TL;DR: 3D microscopic analysis was performed on an intact cochlea of newborn and juvenile pigs with a volume up to ∼ 250 mm3 using the BoneClear tissue clearing technique, which renders the bone transparent, to support the growing effort to regenerate cochlear tissues and assist with basic research to advance cures for hearing impairments. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: December 11, 2020

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