@article{pasqui_cicaloni_tinti_guiotto_tinti_mori_bruttini_hayek_pecorelli_salvini_et al._2024, title={A proteomic approach to investigate the role of the MECP2 gene mutation in Rett syndrome redox regulatory pathways}, volume={752}, ISSN={["1096-0384"]}, DOI={10.1016/j.abb.2023.109860}, abstractNote={Mutations in the X-linked methyl-CpG-binding 2 (MECP2) gene lead to Rett Syndrome (RTT; OMIM 312750), a devasting neurodevelopmental disorder. RTT clinical manifestations are complex and with different degrees of severity, going from autistic-like behavior to loss of acquired speech, motor skills and cardiac problems. Furthermore, the correlation between the type of MECP2 mutation and the clinical phenotype is still not fully understood. Contextually, different genotypes can differently affect the patient's phenotype and omics methodologies such as proteomics could be an important tool for a molecular characterization of genotype/phenotype correlation. The aim of our study was focused on evaluating RTT oxidative stress (OS) responses related to specific MECP2 gene mutations by using proteomics and bioinformatics approaches. Primary fibroblasts isolated from patients affected by R133C and R255× mutations were compared to healthy controls (HC). After clustering primary dermal fibroblasts based on their specific MECP2 mutations, fibroblast-derived protein samples were qualitative and quantitative analyzed, using a label free quantification (LFQ) analysis by mass spectrometry (MS), achieving a preliminary correlation for RTT genotype/phenotype. Among the identified proteins involved in redox regulation pathways, NAD(P)H:quinone acceptor oxidoreductase 1 (NQO1) was found to be absent in R255× cells, while it was present in R133C and in HC fibroblasts. Moreover, NQO1 aberrant gene regulation was also confirmed when cells were challenged with 100 μM hydrogen peroxide (H2O2). In conclusion, by employing a multidisciplinary approach encompassing proteomics and bioinformatics analyses, as well as molecular biology assays, the study uncovered phenotypic responses linked to specific MECP2 gene mutations. These findings contribute to a better understanding of the complexity of RTT molecular pathways, confirming the high heterogeneity among the patients.}, journal={ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS}, author={Pasqui, Arianna and Cicaloni, Vittoria and Tinti, Laura and Guiotto, Anna and Tinti, Cristina and Mori, Alessia and Bruttini, Marco and Hayek, Joussef and Pecorelli, Alessandra and Salvini, Laura and et al.}, year={2024}, month={Feb} }
 @article{ferrara_yan_pecorelli_guiotto_colella_pasqui_ivarrson_lynch_anderias_choundhary_et al._2024, title={Combined exposure to UV and PM affect skin oxinflammatory responses and it is prevented by antioxidant mix topical application: Evidences from clinical study}, volume={4}, ISSN={["1473-2165"]}, DOI={10.1111/jocd.16321}, abstractNote={Abstract Background Exposure to environmental stressors like particulate matter (PM) and ultraviolet radiation (UV) induces cutaneous oxidative stress and inflammation and leads to skin barrier dysfunction and premature aging. Metals like iron or copper are abundant in PM and are known to contribute to reactive oxygen species (ROS) production. Aims Although it has been suggested that topical antioxidant may be able to help in preventing and/or reducing outdoor skin damage, limited clinical evidence under real‐life exposure conditions have been reported. The aim of the present study was to evaluate the ability of a topical serum containing 15% ascorbic acid, 0.5% ferulic acid, and 1% tocopherol (CF Mix) to prevent oxinflammatory skin damage and premature aging induced by PM + UV in a human clinical trial. Methods A 4‐day single‐blinded, clinical study was conducted on the back of 15 females (18–40 years old). During the 4 consecutive days, the back test zones were treated daily with or without the CF Mix, followed by with/without 2 h of PM and 5 min of UV daily exposure. Results Application of the CF Mix prevented PM + UV‐induced skin barrier perturbation (Involucrin and Loricrin), lipid peroxidation (4HNE), inflammatory markers (COX2, NLRP1, and AhR), and MMP9 activation. In addition, CF Mix was able to prevent Type I Collagen loss. Conclusion This is the first human study confirming the multipollutants cutaneous damage and suggesting the utility of a daily antioxidant topical application to prevent pollution induced skin damage.}, journal={JOURNAL OF COSMETIC DERMATOLOGY}, author={Ferrara, Francesca and Yan, Xi and Pecorelli, Alessandra and Guiotto, Anna and Colella, Sante and Pasqui, Arianna and Ivarrson, John and Lynch, Stephen and Anderias, Sara and Choundhary, Hina and et al.}, year={2024}, month={Apr} }
 @article{ivarsson_ferrara_vallese_guiotto_colella_pecorelli_valacchi_2023, title={Comparison of Pollutant Effects on Cutaneous Inflammasomes Activation}, volume={24}, ISSN={["1422-0067"]}, url={https://doi.org/10.3390/ijms242316674}, DOI={10.3390/ijms242316674}, abstractNote={The skin is the outermost layer of the body and, therefore, is exposed to a variety of stressors, such as environmental pollutants, known to cause oxinflammatory reactions involved in the exacerbation of several skin conditions. Today, inflammasomes are recognized as important modulators of the cutaneous inflammatory status in response to air pollutants and ultraviolet (UV) light exposure. In this study, human skin explants were exposed to the best-recognized air pollutants, such as microplastics (MP), cigarette smoke (CS), diesel engine exhaust (DEE), ozone (O3), and UV, for 1 or 4 days, to explore how each pollutant can differently modulate markers of cutaneous oxinflammation. Exposure to environmental pollutants caused an altered oxidative stress response, accompanied by increased DNA damage and signs of premature skin aging. The effect of specific pollutants being able to exert different inflammasomes pathways (NLRP1, NLRP3, NLRP6, and NLRC4) was also investigated in terms of scaffold formation and cell pyroptosis. Among all environmental pollutants, O3, MP, and UV represented the main pollutants affecting cutaneous redox homeostasis; of note, the NLRP1 and NLRP6 inflammasomes were the main ones modulated by these outdoor stressors, suggesting their role as possible molecular targets in preventing skin disorders and the inflammaging events associated with environmental pollutant exposure.}, number={23}, journal={INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}, author={Ivarsson, John and Ferrara, Francesca and Vallese, Andrea and Guiotto, Anna and Colella, Sante and Pecorelli, Alessandra and Valacchi, Giuseppe}, year={2023}, month={Dec} }
 @article{hoskin_grace_guiotto_pecorelli_valacchi_lila_2023, title={Development of Spray Dried Spirulina Protein-Berry Pomace Polyphenol Particles to Attenuate Pollution-Induced Skin Damage: A Convergent Food-Beauty Approach}, volume={12}, ISSN={["2076-3921"]}, url={https://doi.org/10.3390/antiox12071431}, DOI={10.3390/antiox12071431}, abstractNote={Spray drying (SD) microencapsulation of phytochemicals from berry pomaces with Spirulina protein (SP) was incorporated into a cosmeceutical topical formulation to mitigate pollution skin damage. Initially, microparticles produced with SP and polyphenols recovered from fruit pomaces (elderberry SP-EB and muscadine grape SP-MG) were characterized regarding physicochemical and phytochemical content (polyphenol load, carotenoid and phycocyanin contents and antioxidant activity). SP had low total phenolic content (7.43 ± 0.23 mg GAE/g DW), but complexation with elderberry or muscadine grape pomaces polyphenols led to a substantial increase (27.63 ± 1.15 SP-EB and 111.0 ± 2.6 mg GAE/g DW SP-MG). SP-MG particles had higher anthocyanin (26.87 ± 1.25 mg/g) and proanthocyanidin (9.02 ± 0.74 mg/g) contents compared to SP-EB particles. SP-MG were prioritized to prepare a topical gel to attenuate skin oxinflammatory markers and prevent skin barrier disruption using ex vivo human biopsies exposed to diesel engine exhaust (DEE). The immunofluorescence results showed increased oxidative protein damage and inflammation associated with impaired skin barrier function after DEE exposure while topical application of gel formulated with SP-MG mitigated these effects. Overall, this study demonstrated that protein-polyphenol complexation is a synergistic strategy to stabilize and deliver residual fruit/algae phytoactives into cosmeceutical products for skin health applications.}, number={7}, journal={ANTIOXIDANTS}, author={Hoskin, Roberta Targino and Grace, Mary H. and Guiotto, Anna and Pecorelli, Alessandra and Valacchi, Giuseppe and Lila, Mary Ann}, year={2023}, month={Jul} }
 @article{ferrara_pecorelli_guiotto_vallese_cordone_benedusi_cervellati_valacchi_2023, title={INFLAMMASOMES REGULATION BY ENVIRONMENTAL POLLUTANTS IN HUMAN SKIN}, volume={201}, ISSN={["1873-4596"]}, url={http://dx.doi.org/10.1016/j.freeradbiomed.2023.03.158}, DOI={10.1016/j.freeradbiomed.2023.03.158}, journal={FREE RADICAL BIOLOGY AND MEDICINE}, author={Ferrara, Francesca and Pecorelli, Alessandra and Guiotto, Anna and Vallese, Andrea and Cordone, Valeria and Benedusi, Mascia and Cervellati, Franco and Valacchi, Giuseppe}, year={2023}, month={May}, pages={38–39} }
 @article{benedusi_kerob_guiotto_cervellati_ferrara_pambianchi_2023, title={Topical Application of M89PF Containing Vichy Mineralising Water and Probiotic Fractions Prevents Cutaneous Damage Induced by Exposure to UV and O3}, volume={16}, ISSN={["1178-7015"]}, DOI={10.2147/CCID.S414011}, abstractNote={Purpose Exposure of the skin to ultraviolet radiation (UV) or ozone (O3) results in stressed skin, leading to the alteration of the skin physical barrier and defence functions. In this work, the preventive benefit of a dermocosmetic, M89PF, containing Vichy mineralising water, probiotic fractions, antioxidant vitamins and hyaluronic acid, in the alteration of skin physical barrier and skin defence functions after exposure to O3 and UV, alone or combined, was assessed. Methods Untreated and treated (M89PF) skin explants were exposed to O3, to UV rays or to O3+UV. Immunofluorescence was performed for skin barrier, oxidative stress, and inflammatory markers after one and four days of exposure to the pollutants. Results M89PF significantly (p≤0.05) prevented the decrease of the expression level of different skin barrier markers, and significantly (p≤0.05) prevented the induction of OxInflammatory markers and inflammasome components by UV, O3, or both combined. Conclusion M89PF prevents skin barrier damage, as well as oxidative stress and inflammatory markers induced by exposome factors, such as UV, O3, or both combined.}, journal={CLINICAL COSMETIC AND INVESTIGATIONAL DERMATOLOGY}, author={Benedusi, Mascia and Kerob, Delphine and Guiotto, Anna and Cervellati, Franco and Ferrara, Francesca and Pambianchi, Erika}, year={2023}, pages={1769–1776} }