@article{yamamoto_huang_anholt_mackay_2024, title={Article The genetic basis of variation in Drosophila melanogaster mating behavior}, volume={27}, ISSN={["2589-0042"]}, DOI={10.1016/j.isci.2024.109837}, abstractNote={Mating behavior is an essential fitness trait. We used the inbred, sequenced lines of the}, number={5}, journal={ISCIENCE}, author={Yamamoto, Akihiko and Huang, Wen and Anholt, Robert R. H. and Mackay, Trudy F. C.}, year={2024}, month={May} }
 @article{yadav_butler_yamamoto_patil_lloyd_scott_2023, title={CRISPR/Cas9-based split homing gene drive targeting
            <i>doublesex
            for population suppression of the global fruit pest
            <i>Drosophila suzukii}, volume={120}, ISSN={0027-8424 1091-6490}, url={http://dx.doi.org/10.1073/pnas.2301525120}, DOI={10.1073/pnas.2301525120}, abstractNote={
            Genetic-based methods offer environmentally friendly species-specific approaches for control of insect pests. One method, CRISPR homing gene drive that target genes essential for development, could provide very efficient and cost-effective control. While significant progress has been made in developing homing gene drives for mosquito disease vectors, little progress has been made with agricultural insect pests. Here, we report the development and evaluation of split homing drives that target the
            doublesex
            (
            dsx
            ) gene in
            Drosophila suzukii
            , an invasive pest of soft-skinned fruits. The drive component, consisting of
            dsx
            single guide RNA and DsRed genes, was introduced into the female-specific exon of
            dsx
            , which is essential for function in females but not males. However, in most strains, hemizygous females were sterile and produced the male
            dsx
            transcript. With a modified homing drive that included an optimal splice acceptor site, hemizygous females from each of the four independent lines were fertile. High transmission rates of the DsRed gene (94 to 99%) were observed with a line that expressed Cas9 with two nuclear localization sequences from the
            D. suzukii nanos
            promoter. Mutant alleles of
            dsx
            with small in-frame deletions near the Cas9 cut site were not functional and thus would not provide resistance to drive. Finally, mathematical modeling showed that the strains could be used for suppression of lab cage populations of
            D. suzukii
            with repeated releases at relatively low release ratios (1:4). Our results indicate that the split CRISPR homing gene drive strains could potentially provide an effective means for control of
            D. suzukii
            populations.
          }, number={25}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Yadav, Amarish K. and Butler, Cole and Yamamoto, Akihiko and Patil, Anandrao A. and Lloyd, Alun L. and Scott, Maxwell J.}, year={2023}, month={Jun} }
 @article{yadav_asokan_yamamoto_patil_scott_2023, title={Expansion of the genetic toolbox for manipulation of the global crop pest <scp><i>Drosophila suzukii</i></scp>: Isolation and assessment of eye colour mutant strains}, volume={10}, ISSN={0962-1075 1365-2583}, url={http://dx.doi.org/10.1111/imb.12879}, DOI={10.1111/imb.12879}, abstractNote={Abstract}, journal={Insect Molecular Biology}, publisher={Wiley}, author={Yadav, Amarish K. and Asokan, Ramasamy and Yamamoto, Akihiko and Patil, Anandrao A. and Scott, Maxwell J.}, year={2023}, month={Oct} }
 @article{lyman_lyman_yamamoto_huang_harbison_zhou_anholt_mackay_2023, title={Natural genetic variation in a dopamine receptor is associated with variation in female fertility in Drosophila melanogaster}, volume={290}, ISSN={["1471-2954"]}, DOI={10.1098/rspb.2023.0375}, abstractNote={
            Fertility is a major component of fitness but its genetic architecture remains poorly understood. Using a full diallel cross of 50
            Drosophila
            Genetic Reference Panel inbred lines with whole genome sequences, we found substantial genetic variation in fertility largely attributable to females. We mapped genes associated with variation in female fertility by genome-wide association analysis of common variants in the fly genome. Validation of candidate genes by RNAi knockdown confirmed the role of the dopamine 2-like receptor (
            Dop2R
            ) in promoting egg laying. We replicated the
            Dop2R
            effect in an independently collected productivity dataset and showed that the effect of the
            Dop2R
            variant was mediated in part by regulatory gene expression variation. This study demonstrates the strong potential of genome-wide association analysis in this diverse panel of inbred strains and subsequent functional analyses for understanding the genetic architecture of fitness traits.
          }, number={1996}, journal={PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES}, author={Lyman, Richard F. and Lyman, Rachel A. and Yamamoto, Akihiko and Huang, Wen and Harbison, Susan T. and Zhou, Shanshan and Anholt, Robert R. H. and Mackay, Trudy F. C.}, year={2023}, month={Apr} }
 @article{yamamoto_yadav_scott_2022, title={Evaluation of Additional Drosophila suzukii Male-Only Strains Generated Through Remobilization of an FL19 Transgene}, volume={10}, ISSN={["2296-4185"]}, url={https://europepmc.org/articles/PMC8965018}, DOI={10.3389/fbioe.2022.829620}, abstractNote={Drosophila suzukii (D. suzukii) (Matsumura, 1931; Diptera: Drosophilidae), also known as spotted wing Drosophila, is a worldwide pest of fruits with soft skins such as blueberries and cherries. Originally from Asia, D. suzukii is now present in the Americas and Europe and has become a significant economic pest. Growers largely rely on insecticides for the control of D. suzukii. Genetic strategies offer a species-specific environmentally friendly way for suppression of D. suzukii populations. We previously developed a transgenic strain of D. suzukii that produced only males on a diet that did not contain tetracycline. The strain carried a single copy of the FL19 construct on chromosome 3. Repeated releases of an excess of FL19 males led to suppression of D. suzukii populations in laboratory cage trials. Females died as a consequence of overexpression of the tetracycline transactivator (tTA) and tTA-activated expression of the head involution defective proapoptotic gene. The aim of this study was to generate additional male-only strains that carried two copies of the FL19 transgene through crossing the original line with a piggyBac jumpstarter strain. Males that carried either two chromosome 3 or a singleX-linked transgene were identified through stronger expression of the red fluorescent protein marker gene. The brighter fluorescence of the X-linked lines was likely due to dosage compensation of the red fluorescent protein gene. In total, four X-linked lines and eleven lines with two copies on chromosome 3 were obtained, of which five were further examined. All but one of the strains produced only males on a diet without tetracycline. When crossed with wild type virgin females, all of the five two copy autosomal strains examined produced only males. However, the single copy X-linked lines did not show dominant female lethality. Five of the autosomal lines were further evaluated for productivity (egg to adult) and male competition. Based on these results, the most promising lines have been selected for future population suppression experiments with strains from different geographical locations.}, journal={FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY}, author={Yamamoto, Akihiko and Yadav, Amarish K. and Scott, Maxwell J.}, year={2022}, month={Mar} }
 @article{li_yamamoto_belikoff_berger_griffith_scott_2021, title={A conditional female lethal system for genetic suppression of the global fruit crop pest <scp><i>Drosophila suzukii}, volume={77}, ISSN={1526-498X 1526-4998}, url={http://dx.doi.org/10.1002/ps.6530}, DOI={10.1002/ps.6530}, abstractNote={Abstract}, number={11}, journal={Pest Management Science}, publisher={Wiley}, author={Li, Fang and Yamamoto, Akihiko and Belikoff, Esther J and Berger, Amy and Griffith, Emily H and Scott, Maxwell J}, year={2021}, month={Jul}, pages={4915–4922} }
 @article{tallo_duncan_yamamoto_slaydon_arya_turlapati_mackay_carbone_2021, title={Heat shock proteins and small nucleolar RNAs are dysregulated in a Drosophila model for feline hypertrophic cardiomyopathy}, volume={11}, ISSN={["2160-1836"]}, DOI={10.1093/g3journal/jkaa014}, abstractNote={Abstract}, number={1}, journal={G3-GENES GENOMES GENETICS}, author={Tallo, Christian A. and Duncan, Laura H. and Yamamoto, Akihiko H. and Slaydon, Joshua D. and Arya, Gunjan H. and Turlapati, Lavanya and Mackay, Trudy F. C. and Carbone, Mary A.}, year={2021}, month={Jan} }
 @article{yanagawa_huang_yamamoto_wada-katsumata_schal_mackay_2020, title={Genetic Basis of Natural Variation in Spontaneous Grooming in Drosophila melanogaster}, volume={10}, ISSN={["2160-1836"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-85090276700&partnerID=MN8TOARS}, DOI={10.1534/g3.120.401360}, abstractNote={Abstract}, number={9}, journal={G3-GENES GENOMES GENETICS}, author={Yanagawa, Aya and Huang, Wen and Yamamoto, Akihiko and Wada-Katsumata, Ayako and Schal, Coby and Mackay, Trudy F. C.}, year={2020}, month={Sep}, pages={3453–3460} }
 @article{huang_massouras_inoue_peiffer_ramia_tarone_turlapati_zichner_zhu_lyman_et al._2014, title={Natural variation in genome architecture among 205 Drosophila melanogaster Genetic Reference Panel lines}, volume={24}, number={7}, journal={Genome Research}, author={Huang, W. and Massouras, A. and Inoue, Y. and Peiffer, J. and Ramia, M. and Tarone, A. M. and Turlapati, L. and Zichner, T. and Zhu, D. H. and Lyman, R. F. and et al.}, year={2014}, pages={1193–1208} }
 @article{sandukas_yamamoto_rabiei_2011, title={Osteoblast adhesion to functionally graded hydroxyapatite coatings doped with silver}, volume={97A}, ISSN={["1552-4965"]}, DOI={10.1002/jbm.a.33081}, abstractNote={Abstract}, number={4}, journal={JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A}, author={Sandukas, Stefan and Yamamoto, Akiko and Rabiei, Afsaneh}, year={2011}, month={Jun}, pages={490–497} }
 @article{carbone_yamamoto_huang_lyman_meadors_yamamoto_anholt_mackay, title={Genetic architecture of natural variation in visual senescence in Drosophila}, volume={113}, number={43}, journal={Proceedings of the National Academy of Sciences of the United States of America}, author={Carbone, M. A. and Yamamoto, A. and Huang, W. and Lyman, R. A. and Meadors, T. B. and Yamamoto, R. and Anholt, R. R. H. and Mackay, T. F. C.}, pages={E6620–6629} }
 @article{magwire_yamamoto_carbone_roshina_symonenko_pasyukova_morozova_mackay, title={Quantitative and molecular genetic analyses of mutations increasing Drosophila life span}, volume={6}, number={7}, journal={PLoS Genetics}, author={Magwire, M. M. and Yamamoto, A. and Carbone, M. A. and Roshina, N. V. and Symonenko, A. V. and Pasyukova, E. G. and Morozova, T. V. and Mackay, T. F. C.} }
 @article{mackay_richards_stone_barbadilla_ayroles_zhu_casillas_han_magwire_cridland_et al., title={The Drosophila melanogaster genetic reference panel}, volume={482}, number={7384}, journal={Nature}, author={Mackay, T. F. C. and Richards, S. and Stone, E. A. and Barbadilla, A. and Ayroles, J. F. and Zhu, D. H. and Casillas, S. and Han, Y. and Magwire, M. M. and Cridland, J. M. and et al.}, pages={173–178} }
 @article{zhou_morozova_hussain_luoma_mccoy_yamamoto_mackay_anholt, title={The genetic basis for variation in sensitivity to lead toxicity in Drosophila melanogaster}, volume={124}, number={7}, journal={Environmental Health Perspectives}, author={Zhou, S. S. and Morozova, T. V. and Hussain, Y. N. and Luoma, S. E. and McCoy, L. and Yamamoto, A. and Mackay, T. F. C. and Anholt, R. R. H.}, pages={1062–1070} }