@article{gayen_nichols_williams_2020, title={An artificial pathway for polyketide biosynthesis}, volume={3}, ISSN={2520-1158}, url={http://dx.doi.org/10.1038/s41929-020-0483-4}, DOI={10.1038/s41929-020-0483-4}, number={7}, journal={Nature Catalysis}, publisher={Springer Science and Business Media LLC}, author={Gayen, Anuran K. and Nichols, Lindsay and Williams, Gavin J.}, year={2020}, month={Jul}, pages={536–538} }
 @article{malico_calzini_gayen_williams_2020, title={Synthetic biology, combinatorial biosynthesis, and chemo-enzymatic synthesis of isoprenoids (September, 10.1007/s10295-020-02306-3, 2020)}, volume={47}, ISSN={["1476-5535"]}, url={https://doi.org/10.1007/s10295-020-02327-y}, DOI={10.1007/s10295-020-02327-y}, abstractNote={Abstract}, number={12}, journal={JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY}, publisher={Springer Science and Business Media LLC}, author={Malico, Alexandra A. and Calzini, Miles A. and Gayen, Anuran K. and Williams, Gavin J.}, year={2020}, month={Dec}, pages={1181–1181} }
 @article{malico_calzini_gayen_williams_2020, title={Synthetic biology, combinatorial biosynthesis, and chemo‑enzymatic synthesis of isoprenoids}, volume={47}, ISSN={1367-5435 1476-5535}, url={http://dx.doi.org/10.1007/s10295-020-02306-3}, DOI={10.1007/s10295-020-02306-3}, abstractNote={Abstract}, number={9-10}, journal={Journal of Industrial Microbiology & Biotechnology}, publisher={Springer Science and Business Media LLC}, author={Malico, Alexandra A. and Calzini, Miles A. and Gayen, Anuran K. and Williams, Gavin J.}, year={2020}, month={Sep}, pages={675–702} }
 @article{kalkreuter_keeler_malico_bingham_gayen_williams_2019, title={Development of a Genetically Encoded Biosensor for Detection of Polyketide Synthase Extender Units in Escherichia coli}, volume={8}, ISSN={2161-5063 2161-5063}, url={http://dx.doi.org/10.1021/acssynbio.9b00078}, DOI={10.1021/acssynbio.9b00078}, abstractNote={The scaffolds of polyketides are constructed via assembly of extender units based on malonyl-CoA and its derivatives that are substituted at the C2-position with diverse chemical functionality. Subsequently, a transcription-factor-based biosensor for malonyl-CoA has proven to be a powerful tool for detecting malonyl-CoA, facilitating the dynamic regulation of malonyl-CoA biosynthesis and guiding high-throughput engineering of malonyl-CoA-dependent processes. Yet, a biosensor for the detection of malonyl-CoA derivatives has yet to be reported, severely restricting the application of high-throughput synthetic biology approaches to engineering extender unit biosynthesis and limiting the ability to dynamically regulate the biosynthesis of polyketide products that are dependent on such α-carboxyacyl-CoAs. Herein, the FapR biosensor was re-engineered and optimized for a range of mCoA concentrations across a panel of E. coli strains. The effector specificity of FapR was probed by cell-free transcription-translation, revealing that a variety of non-native and non-natural acyl-thioesters are FapR effectors. This FapR promiscuity proved sufficient for the detection of the polyketide extender unit methylmalonyl-CoA in E. coli, providing the first reported genetically encoded biosensor for this important metabolite. As such, the previously unknown broad effector promiscuity of FapR provides a platform to develop new tools and approaches that can be leveraged to overcome limitations of pathways that construct diverse α-carboxyacyl-CoAs and those that are dependent on them, including biofuels, antibiotics, anticancer drugs, and other value-added products.}, number={6}, journal={ACS Synthetic Biology}, publisher={American Chemical Society (ACS)}, author={Kalkreuter, Edward and Keeler, Aaron M. and Malico, Alexandra A. and Bingham, Kyle S. and Gayen, Anuran K. and Williams, Gavin J.}, year={2019}, month={May}, pages={1391–1400} }
 @article{ghosh_duttagupta_bose_banerjee_gayen_sinha_2019, title={Synthesis and anticancer activities of proline-containing cyclic peptides and their linear analogs and congeners}, volume={49}, ISSN={["1532-2432"]}, DOI={10.1080/00397911.2018.1550201}, abstractNote={Abstract A solution phase method was adopted for the synthesis of proline-containing cyclic pentapeptide 2 and total synthesis of naturally occurring cyclic heptapeptide Reniochalistatin B 3. For the synthesis of 3, both divergent and convergent strategies were used to improve the overall yield from 12 to 25%. Different N and C terminal modified linear analogs and congeners of 2 and 3 were synthesized. Both cyclic peptides 2 and 3 and their linear analogs/congeners were evaluated for anti-cancer activity against HeLa cell line, among which pentapeptide 2 h and hexapeptide 3n with N-terminal protected hexafluoroisopropyl carbamates (HFIPC) interestingly showed higher cytotoxicity with an IC50 of 2.73 and 4.3 µM, respectively compared to their Boc-protected analogs 2a (IC50 20 µM) and 3c (IC50 38.51 µM) and cyclic peptides 2 (>100 µM) and 3 (47 µM). These results were further validated by biological experiments such as colony formation and wound healing assays. Graphical Abstract}, number={2}, journal={SYNTHETIC COMMUNICATIONS}, author={Ghosh, Keshab Ch and Duttagupta, Indranil and Bose, Chandra and Banerjee, Priyanjalee and Gayen, Anuran Kumar and Sinha, Surajit}, year={2019}, month={Jan}, pages={221–236} }