@article{bittner_sweigard_mila_2017, title={Assessing the resistance potential of Phytophthora nicotianae, the causal agent of black shank of tobacco, to oxathiopropalin with laboratory mutants}, volume={102}, journal={Crop Protection}, author={Bittner, R. J. and Sweigard, J. A. and Mila, A. L.}, year={2017}, pages={63–71} } @article{bittner_arellano_mila_2016, title={Effect of temperature and resistance of tobacco cultivars to the progression of bacterial wilt, caused by Ralstonia solanacearum}, volume={408}, ISSN={["1573-5036"]}, DOI={10.1007/s11104-016-2938-6}, number={1-2}, journal={PLANT AND SOIL}, author={Bittner, R. J. and Arellano, C. and Mila, A. L.}, year={2016}, month={Nov}, pages={299–310} } @article{bittner_mila_2016, title={Effects of oxathiapiprolin on Phytophthora nicotianae, the causal agent of black shank of tobacco}, volume={81}, journal={Crop Protection}, author={Bittner, R. J. and Mila, A. L.}, year={2016}, pages={57–64} } @misc{yuen_mila_2015, title={Landscape-scale disease risk quantification and prediction}, volume={53}, journal={Annual review of phytopathology, vol 53}, author={Yuen, J. and Mila, A.}, year={2015}, pages={471–484} } @inproceedings{mila_ngugi_2011, title={A Bayesian approach to meta-analysis of plant pathology studies}, volume={101}, number={1}, booktitle={Phytopathology}, author={Mila, A. L. and Ngugi, H. K.}, year={2011}, pages={42–51} } @article{mila_2011, title={Explaining loss caused by tomato spotted wilt virus on tobacco with boreal winter weather: A Bayesian approach}, volume={101}, number={4}, journal={Phytopathology}, author={Mila, A. L.}, year={2011}, pages={462–469} } @article{savary_mila_willocquet_esker_carisse_mcroberts_2011, title={Risk factors for crop health under global change and agricultural shifts: a framework of analyses using rice in tropical and subtropical asia as a model}, volume={101}, number={6}, journal={Phytopathology}, author={Savary, S. and Mila, A. and Willocquet, L. and Esker, P. D. and Carisse, O. and McRoberts, N.}, year={2011}, pages={696–709} } @article{antonopoulos_mila_2011, title={Susceptibility of stem and root tissues of tobacco cultivars carrying or not the Ph gene to Pythium stem rot, caused by Pythium aphanidermatum}, volume={30}, number={3}, journal={Crop Protection}, author={Antonopoulos, D. F. and Mila, A. L.}, year={2011}, pages={379–383} } @article{cherry_mila_2011, title={Temporal progress and control of tomato spotted wilt virus in flue-cured tobacco}, volume={30}, number={5}, journal={Crop Protection}, author={Cherry, K. R. and Mila, A. L.}, year={2011}, pages={539–546} } @article{antonopoulos_melton_mila_2010, title={Effects of Chemical Control, Cultivar Resistance, and Structure of Cultivar Root System on Black Shank Incidence of Tobacco}, volume={94}, ISSN={["1943-7692"]}, DOI={10.1094/pdis-94-5-0613}, abstractNote={ Black shank, caused by the hemibiotrophic oomycete Phytophthora parasitica var. nicotianae, is a major disease of tobacco (Nicotiana tabacum). The rise of race 1 in the late 1990s, after extensive cropping of cultivars possessing the Php gene, confirming immunity to race 0 of P. parasitica var. nicotianae, imposed new challenges to black shank management. The effects of tobacco cultivars and chemical controls with mefenoxam (Ridomil Gold) on black shank incidence were investigated in naturally infested fields. Twenty-five cultivars were tested and the highest resistance for races 0 and 1 of P. parasitica var. nicotianae was provided by RJR 75 and SP 227 based on field and laboratory studies. When race 1 was prevalent, mefenoxam was effective to control black shank. An initial application at an early stage of tobacco growth, such as a few days before or after transplant, was essential to successfully control the disease. In greenhouse experiments, cultivars carrying the Php gene produced fewer and shorter adventitious roots than cultivars possessing only partial resistance to all races of P. parasitica var. nicotianae. Strategies such as use of mefenoxam, especially at an early stage, when adventitious roots are emerging, and planting a cultivar with high partial resistance or possessing the Ph gene when race 1 or race 0, respectively, predominates are critical factors in reducing loss due to P. parasitica var. nicotianae. }, number={5}, journal={PLANT DISEASE}, author={Antonopoulos, Dimitrios F. and Melton, Thomas and Mila, Asimina L.}, year={2010}, month={May}, pages={613–620} } @article{mila_yang_2008, title={Effects of fluctuating soil temperature and water potential on sclerotia germination and apothecial production of Sclerotinia sclerotiorum}, volume={92}, ISSN={["1943-7692"]}, DOI={10.1094/PDIS-92-1-0078}, abstractNote={ The effects of fluctuating soil temperature and water potential on sclerotial germination and apothecial production by Sclerotinia sclerotiorum were investigated in growth chamber experiments. In the temperature experiments, temperature fluctuations of 4, 8, 12, and 16°C around a median of 20°C, and a constant of 20°C, were tested. Daily temperature fluctuations of 8°C resulted in highest levels of sclerotial germination and apothecial production. The earliest appearance of apothecia occurred in the 8°C fluctuation treatment, 24 days after the start of the experiment. Sclerotia in the 12°C fluctuation treatment germinated last; its first sclerotium germinated 44 days after experiment initiation. For the soil water potential experiments, constant saturation (approximately –0.001 MPa) and three levels of soil water potential fluctuation from saturation—“low” (–0.03 to –0.04 MPa), “medium” (–0.06 to –0.07 MPa), and “high” (–0.09 to –0.1 MPa)—were tested. Constant saturation yielded the highest number of germinated sclerotia and apothecia. All soil water potential fluctuations were detrimental to sclerotial germination and apothecial production, with sclerotial germination under fluctuating moisture conditions less than a tenth of that occurring under constant saturation. The first sclerotium in the constant saturation treatment germinated in 35 days; however, 76 days were required in the high soil water potential fluctuation treatment. }, number={1}, journal={PLANT DISEASE}, author={Mila, A. L. and Yang, X. B.}, year={2008}, month={Jan}, pages={78–82} } @article{gutierrez_mila_2007, title={A rapid technique for determination of races of Phytophthora nicotianae on tobacco}, volume={91}, ISSN={["1943-7692"]}, DOI={10.1094/PDIS-91-8-0985}, abstractNote={ A laboratory technique for determining races of Phytophthora nicotianae on tobacco (Nicotiana tabacum) was developed and compared with a commonly used greenhouse method. The laboratory technique was based on production and inoculation of tobacco seedlings in tissue culture plates. Three P. nicotianae isolates from North Carolina previously determined as race 0 and 1 were used. Four tobacco cultivars and two breeding lines with different types of resistance were used as differential cultivars: K-326, K-346, NC-71, NC-1071, L8, and Ky14xL8. Plants were evaluated 7 and 14 days after inoculation. Five differential cultivars (K-326, K-346, NC-1071, NC-71, and L8) were determined to be sufficient to differentiate races 0 and 1. Cv. Ky14xL8 was ineffective for differentiation of races and produced inconsistent results. The laboratory technique was as effective as the greenhouse technique for distinguishing different races of P. nicotianae for every isolate in all experiments. Additionally, the most reliable results for both methods were obtained when evaluations were made 14 days after inoculation. The laboratory technique was validated with 21 isolates collected from four counties in North Carolina. The laboratory technique produced results 2 weeks faster than the greenhouse technique and required significantly less space and labor than the greenhouse technique for the same number of isolates. Additionally, the larger number of seedlings used in the laboratory technique increased the robustness of the results, especially for isolates for which race identification was unclear with the greenhouse technique. We propose that the laboratory technique has the potential for evaluation of tobacco resistance in other pathosystems as well. }, number={8}, journal={PLANT DISEASE}, author={Gutierrez, Walter A. and Mila, Asimina L.}, year={2007}, month={Aug}, pages={985–989} }