@article{amerson_nelson_kubisiak_kuhlman_garcia_2015, title={Identification of Nine Pathotype-Specific Genes Conferring Resistance to Fusiform Rust in Loblolly Pine (Pinus taeda L.)}, volume={6}, ISSN={["1999-4907"]}, DOI={10.3390/f6082739}, abstractNote={Nearly two decades of research on the host-pathogen interaction in fusiform rust of loblolly pine is detailed. Results clearly indicate that pathotype-specific genes in the host interacting with pathogen avirulence cause resistance as defined by the non-gall phenotype under favorable environmental conditions for disease development. In particular, nine fusiform rust resistance genes (Fr genes) are described here including the specific methods to determine each and their localization on the reference genetic map of loblolly pine. Understanding how these and other apparent Fr genes in loblolly pine and other rust-susceptible pines impact resistance screening, parental and progeny selection, and family and clonal deployment is an important area in forest genetics research and}, number={8}, journal={FORESTS}, author={Amerson, Henry V. and Nelson, C. Dana and Kubisiak, Thomas L. and Kuhlman, E. George and Garcia, Saul A.}, year={2015}, month={Aug}, pages={2739–2761} }
 @article{kubisiak_anderson_amerson_smith_davis_nelson_2011, title={A genomic map enriched for markers linked to Avr1 in Cronartium quercuum f.sp. fusiforme}, volume={48}, ISSN={["1096-0937"]}, DOI={10.1016/j.fgb.2010.09.008}, abstractNote={A novel approach is presented to map avirulence gene Avr1 in the basidiomycete Cronartium quercuum f.sp. fusiforme, the causal agent of fusiform rust disease in pines. DNA markers tightly linked to resistance gene Fr1 in loblolly pine tree 10-5 were used to classify 10-5 seedling progeny as either resistant or susceptible. A single dikaryotic isolate (P2) heterozygous at the corresponding Avr1 gene was developed by crossing Fr1 avirulent isolate SC20-21 with Fr1 virulent isolate NC2-40. Bulk basidiospore inoculum derived from isolate P2 was used to challenge the pine progeny. The ability to unambiguously marker classify 10-5 progeny as resistant (selecting for virulence) or susceptible (non-selecting) permitted the genetic mapping of the corresponding Avr1 gene by bulked segregant analysis. Using this approach, 14 genetic markers significantly linked to Avr1 were identified and placed within the context of a genome-wide linkage map produced for isolate P2 using samples from susceptible seedlings.}, number={3}, journal={FUNGAL GENETICS AND BIOLOGY}, author={Kubisiak, Thomas L. and Anderson, Claire L. and Amerson, Henry V. and Smith, Jason A. and Davis, John M. and Nelson, C. Dana}, year={2011}, month={Mar}, pages={266–274} }
 @article{isik_amerson_whetten_garcia_mckeand_2011, title={Interactions of Fr genes and mixed-pathogen inocula in the loblolly pine-fusiform rust pathosystem}, volume={8}, ISSN={1614-2942 1614-2950}, url={http://dx.doi.org/10.1007/s11295-011-0416-0}, DOI={10.1007/s11295-011-0416-0}, number={1}, journal={Tree Genetics & Genomes}, publisher={Springer Science and Business Media LLC}, author={Isik, Fikret and Amerson, Henry V. and Whetten, Ross W. and Garcia, Saul A. and McKeand, Steven E.}, year={2011}, month={Aug}, pages={15–25} }
 @article{huber_amerson_2011, title={Performance of the loblolly pine fusiform rust disease resistance gene (Fr1) in a slashXloblolly pine hybrid family}, volume={7}, ISSN={["1614-2950"]}, DOI={10.1007/s11295-010-0353-3}, abstractNote={Unexpectedly high levels of field susceptibility to the fusiform rust fungus observed for slashXloblolly hybrid families in the Cooperative Forest Genetics Research Program Pine Hybrid Trials led to several hypotheses concerning causation of the observed susceptibility. One of these hypotheses involved the failure of major resistance genes to appropriately function in this particular hybrid combination. This current work, involving the loblolly pine resistance gene Fr1 and a fusiform rust inoculum avirulent against Fr1 resistance in a greenhouse trial, delineates the investigation of major gene resistance for a particular slashXloblolly hybrid family. In this trial, the Fr1 resistance allele, derived from the heterozygous (Fr1/fr1) loblolly male parent and transferred to hybrid offspring that should have been segregating 1:1 for resistance, was fully penetrant. Likewise, in the pure-species loblolly control, the Fr1 resistance allele was again fully penetrant, and the performances of our hybrid family and the loblolly control family (both of which segregated for Fr1 resistance) were comparable. These results by inductive reasoning refute the hypothesis that major resistance genes are not appropriately functional in a slashXloblolly hybrid background.}, number={3}, journal={TREE GENETICS & GENOMES}, author={Huber, Dudley and Amerson, Henry}, year={2011}, month={Jun}, pages={535–540} }
 @article{kayihan_nelson_huber_amerson_white_davis_2010, title={Clonal evaluation for fusiform rust disease resistance: effects of pathogen virulence and disease escape}, volume={40}, ISSN={["0045-5067"]}, DOI={10.1139/x10-045}, abstractNote={ We evaluated the precision of phenotypic classification for fusiform rust resistance of Pinus taeda L. in a clonally propagated population segregating for the pathotype-specific resistance gene Fr1. In all marker-defined Fr1/fr1 clones screened with low complexity or ambient inoculum, marker–trait cosegregation was complete with no exceptions. Uncommon exceptions (4 of 30) in which marker-defined Fr1/fr1 clones screened with high complexity inoculum were diseased were probably due to a low frequency of spores virulent to Fr1 resistance. Marker–trait cosegregation for fr1/fr1 clones was less reliable, as all ramets of a few clones (5 of 29, 3 of 25, and 4 of 16) remained disease-free with low complexity, high complexity, or ambient inoculum, respectively. We termed disease-free fr1/fr1 ramets “escapes”, since the genetics of the host–pathogen interaction predicted them to be diseased. For nonmarker-defined materials, we considered escapes to be disease-free ramets within clones that had at least one diseased ramet. Narrow-sense heritability estimates for escape rate were 29% and 23% for the low and high complexity inocula, respectively, suggesting that genetic variation in the host is an important component of this resistance mechanism. }, number={6}, journal={CANADIAN JOURNAL OF FOREST RESEARCH-REVUE CANADIENNE DE RECHERCHE FORESTIERE}, author={Kayihan, Goegce C. and Nelson, C. Dana and Huber, Dudley A. and Amerson, Henry V. and White, Timothy L. and Davis, John M.}, year={2010}, month={Jun}, pages={1042–1050} }
 @article{nelson_kubisiak_amerson_2010, title={Unravelling and managing fusiform rust disease: a model approach for coevolved forest tree pathosystems}, volume={40}, ISSN={["1439-0329"]}, DOI={10.1111/j.1439-0329.2009.00608.x}, abstractNote={Summary}, number={1}, journal={FOREST PATHOLOGY}, author={Nelson, C. D. and Kubisiak, T. L. and Amerson, H. V.}, year={2010}, month={Feb}, pages={64–72} }
 @article{isik_amerson_whetten_garcia_li_mckeand_2008, title={Resistance of <i>Pinus taeda</i> families under artificial inoculations with diverse fusiform rust pathogen populations and comparison with field trials}, volume={38}, ISSN={0045-5067 1208-6037}, url={http://dx.doi.org/10.1139/x08-111}, DOI={10.1139/X08-111}, abstractNote={ Controlled inoculations with 10 bulk inocula of Cronartium quercuum (Berk) Miyabe ex Shirai f.sp. fusiforme were carried out on open-pollinated progeny of 25 fast-growing Pinus taeda L. parents. The parents had a range of breeding values for resistance to fusiform rust in progeny field trials. There were highly significant differences among the half-sib families in response to inoculations, and these differences were very reproducible; the half-sib family-mean heritability of resistance to controlled inoculation was 0.97. All of the families that were susceptible in the field were susceptible in controlled inoculations, and most (12 of 17) of the field-resistant families were resistant in response to controlled inoculations. Significant pathogenic variability was observed among the different bulk inocula, although this accounted for only 1.9% of the total variation. Genetic differences among families within field-resistant or field-susceptible groups accounted for 13.7% of the total variation. The family by inocula interaction was highly significant, but a single field-resistant family contributed 44% of the total family by inocula interaction variance, and two other field-resistant families also showed significant interactions. }, number={10}, journal={Canadian Journal of Forest Research}, publisher={Canadian Science Publishing}, author={Isik, Fikret and Amerson, Henry V. and Whetten, Ross W. and Garcia, Saul A. and Li, Bailian and McKeand, Steven E.}, year={2008}, month={Oct}, pages={2687–2696} }
 @article{isik_amerson_whetten_garcia_li_mckeand_2008, title={Resistance of Pinus taeda families under artificial inoculations with diverse fusiform rust pathogen populations and comparison with field trials (vol 38, pg 2687, 2008)}, volume={38}, ISSN={["0045-5067"]}, DOI={10.1139/x08-910}, number={12}, journal={CANADIAN JOURNAL OF FOREST RESEARCH-REVUE CANADIENNE DE RECHERCHE FORESTIERE}, author={Isik, Fikret and Amerson, Henry V. and Whetten, Ross W. and Garcia, Saul A. and Li, Bailian and McKeand, Steven E.}, year={2008}, month={Dec}, pages={3151–3151} }
 @misc{lu_sun_amerson_chiang_2007, title={MicroRNAs in loblolly pine (Pinus taeda L.) and their association with fusiform rust gall development}, volume={51}, ISSN={["1365-313X"]}, DOI={10.1111/j.1365-313x.2007.03208.x}, abstractNote={Summary}, number={6}, journal={PLANT JOURNAL}, author={Lu, Shanfa and Sun, Ying-Hsuan and Amerson, Henry and Chiang, Vincent L.}, year={2007}, month={Sep}, pages={1077–1098} }
 @article{myburg_morse_amerson_kubisiak_huber_osborne_garcia_nelson_davis_covert_et al._2006, title={Differential gene expression in loblolly pine (Pinus taeda L.) challenged with the fusiform rust fungus, Cronartium quercuum f.sp fusiforme}, volume={68}, ISSN={["0885-5765"]}, DOI={10.1016/j.pmpp.2006.07.002}, abstractNote={Cronartium quercuum f.sp. fusiforme is the pathogen that incites fusiform rust disease of southern pine species. To date, a number of host resistance genes have been mapped. Although genomic mapping studies have provided valuable information on the genetic basis of disease interactions in this pine-rust pathosystem, the interaction at the molecular level is poorly understood. To further our understanding of this interaction, we implemented a microarray study to examine the differential expression of genes in pathogen-challenged progeny of a full-sib loblolly pine family known to be segregating at a single dominant resistance gene (Fr1). Statistical analyses revealed shifts in gene expression that may reflect discrete stages of gall development.}, number={1-3}, journal={PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY}, author={Myburg, Henrietta and Morse, Alison M. and Amerson, Henry V. and Kubisiak, Thomas L. and Huber, Dudley and Osborne, Jason A. and Garcia, Saul A. and Nelson, C. Dana and Davis, John M. and Covert, Sarah F. and et al.}, year={2006}, pages={79–91} }
 @article{li_amerson_li_2006, title={Genetic models of host-pathogen gene interaction based on inoculation of loblolly pine seedlings with the fusiform rust fungus}, volume={31}, ISSN={["1573-5095"]}, DOI={10.1007/s11056-005-6576-8}, number={2}, journal={NEW FORESTS}, author={Li, H and Amerson, H and Li, BL}, year={2006}, month={Mar}, pages={245–252} }
 @article{li_ghosh_amerson_li_2006, title={Major gene detection for fusiform rust resistance using Bayesian complex segregation analysis in loblolly pine}, volume={113}, ISSN={["0040-5752"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-33747640613&partnerID=MN8TOARS}, DOI={10.1007/s00122-006-0351-x}, abstractNote={The presence of major genes affecting rust resistance of loblolly pine was investigated in a progeny population that was generated with a half-diallel mating of six parents. A Bayesian complex segregation analysis was used to make inference about a mixed inheritance model (MIM) that included polygenic effects and a single major gene effect. Marginalizations were achieved by using Gibbs sampler. A parent block sampling by which genotypes of a parent and its offspring were sampled jointly was implemented to improve mixing. The MIM was compared with a pure polygenic model (PM) using Bayes factor. Results showed that the MIM was a better model to explain the inheritance of rust resistance than the pure PM in the diallel population. A large major gene variance component estimate (> 50% of total variance), indicated the existence of major genes for rust resistance in the studied loblolly pine population. Based on estimations of parental genotypes, it appears that there may be two or more major genes affecting disease phenotypes in this diallel population.}, number={5}, journal={THEORETICAL AND APPLIED GENETICS}, author={Li, Hua and Ghosh, Sujit and Amerson, Henry and Li, Bailian}, year={2006}, month={Sep}, pages={921–929} }
 @inproceedings{isik_mckeand_li_amerson_2006, title={Marker aided selection of loblolly pine clones}, booktitle={Consortium for Plant Biotechnology Symposium}, author={Isik, F. and McKeand, S. and Li, B. and Amerson, H.}, year={2006} }
 @article{kubisiak_amerson_nelson_2005, title={Genetic interaction of the fusiform rust fungus with resistance gene Fr1 in loblolly pine}, volume={95}, ISSN={["0031-949X"]}, DOI={10.1094/PHYTO-95-0376}, abstractNote={ We propose a method for defining DNA markers linked to Cronartium quercuum f. sp. fusiforme avirulence (Avr) genes. However, before this method can be successfully employed, a spore competition study was needed to determine the genetic composition of single pycnial drops and multiple drops on single galls when using the standard inoculation procedure, whether virulent (avr1) basidiospores ever predispose some resistant (Fr1/fr1) trees to infection by avirulent (Avr1) basidiospores, and whether avr1 and Avr1 basidiospores equally infect susceptible (fr1/fr1) trees. Results of this study suggest that multiple infections within a single gall are common using the concentrated basidiospore system, resulting on average in >4 infection events per tree. Due to multiple infections within a single gall, an individual pycnial drop cannot be assumed to consist of spores from only a single haploid pycnium. Roughly 57% of the drops harvested were found to consist of more than one haploid genotype, most likely due to the physical mixing of spores from genetically different pycnia. Most importantly, although multiple infections do occur in the formation of a single gall, there is no evidence to suggest that the genetics of the proposed gene-for-gene interaction are compromised. Only avr1 basidiospores were observed to cause infection on Fr1/fr1 trees, whereas both avr1 and Avr1 basidiospores were observed to cause infection on fr1/fr1 trees, albeit not at equal frequencies. }, number={4}, journal={PHYTOPATHOLOGY}, author={Kubisiak, TL and Amerson, HV and Nelson, CD}, year={2005}, month={Apr}, pages={376–380} }
 @article{heerden_amerson_preisig_wingfield_wingfield_2005, title={Relative pathogenicity of Cryphonectria cubensis on Eucalyptus clones differing in their resistance to C-cubensis}, volume={89}, ISSN={["1943-7692"]}, DOI={10.1094/PD-89-0659}, abstractNote={Cryphonectria cubensis causes a destructive canker disease of Eucalyptus species. Management of this disease is primarily through breeding and selection of disease resistant trees. One means of selecting such trees is by artificial inoculation with the pathogen. In routine screening trials in South Africa, an isolate of C. cubensis, considered to be highly pathogenic, has been used for such inoculations. Although the most resistant clones under natural conditions are the same as those detected in inoculation trials, a question has arisen whether all clones respond similarly to different C. cubensis isolates. Thus, a trial consisting of five clones, known to differ in susceptibility to infection by C. cubensis, was established. These trees were inoculated with nine South African C. cubensis isolates previously shown to differ in pathogenicity. Inoculations showed a significant isolate × clone interaction as well as an “apparent immunity” for one clone × isolate interaction, providing evidence highly suggestive of a vertical resistance component in the pathosystem. Disease screening in this pathosystem has traditionally relied on a single pathogen isolate; however, considering data presented here, future reliance on a single isolate may be inadequate.}, number={6}, journal={PLANT DISEASE}, author={Heerden, SW and Amerson, HV and Preisig, O and Wingfield, BD and Wingfield, MJ}, year={2005}, month={Jun}, pages={659–662} }
 @article{mckeand_amerson_li_mullin_2003, title={Families of loblolly pine that are the most stable for resistance to fusiform rust are the least predictable}, volume={33}, ISSN={["0045-5067"]}, DOI={10.1139/X03-050}, abstractNote={ In an extensive series of trials with open-pollinated families of loblolly pine (Pinus taeda L.), resistance to fusiform rust disease (caused by Cronartium quercuum (Berk.) Miyabe ex Shirai f. sp. fusiforme) at individual test sites was relatively unpredictable for the families deemed most resistant. The most resistant families were also the most stable for performance across test sites, with stability defined as the slope of the regression of family means for rust infection versus site means for rust infection. A family's R-50 value (its predicted rust infection level when the site mean infection is 50%) was correlated to its stability parameter or slope (r = 0.78). On average, any one family's level of infection (% galled) was reasonably predictable for any given infection level at a given site; the average coefficient of determination (r2) was 0.78 for the regression of family means for rust infection versus site means for rust infection. However, the six most stable families for resistance had the lowest r2 values (average r2 = 0.58). We speculated that the lower predictability for the most resistant families was due to interactions of specific resistance genes in these families and corresponding avirulence and (or) virulence levels in the pathogen populations that may differ among sites. Although the predictability of the individual resistant families was relatively low, if these families were bulked into a resistant seed lot, they performed in a more predictable manner with r2 = 0.74 for the regression of the bulk mean versus site means. Bulks of four to six highly resistant families appeared to be a good solution to obtain stable and predictable performance across a range of sites. }, number={7}, journal={CANADIAN JOURNAL OF FOREST RESEARCH-REVUE CANADIENNE DE RECHERCHE FORESTIERE}, author={McKeand, SE and Amerson, HV and Li, B and Mullin, TJ}, year={2003}, month={Jul}, pages={1335–1339} }
 @article{mckeand_li_amerson_1999, title={Genetic variation in fusiform rust resistance in loblolly pine across a wide geographic range}, volume={48}, number={5}, journal={Silvae Genetica}, author={McKeand, S. E. and Li, B. and Amerson, H. V.}, year={1999}, pages={255–260} }
 @misc{amerson_wilcox_sederoff_kuhlman_o'malley_grattapaglia_1999, title={Methods for within family selection of disease resistance in woody perennials using genetic markers}, volume={5,908,978}, number={1999 June 1}, publisher={Washington, DC: U.S. Patent and Trademark Office}, author={Amerson, H. V. and Wilcox, P. and Sederoff, R. R. and Kuhlman, E. G. and O'Malley, D. M. and Grattapaglia, D.}, year={1999} }
 @article{frampton_amerson_leach_1998, title={Tissue culture method affects ex vitro growth and development of loblolly pine}, volume={16}, number={2}, journal={New Forests}, author={Frampton, L. J. and Amerson, H. V. and Leach, G. N.}, year={1998}, pages={125–138} }
 @article{kuhlman_amerson_jordan_pepper_1997, title={Inoculum density and expression of major gene resistance to fusiform rust disease in loblolly pine}, volume={81}, ISSN={["0191-2917"]}, DOI={10.1094/PDIS.1997.81.6.597}, abstractNote={ Inoculum densities of 25 × 103 to 200 × 103 per ml of basidiospores from single aeciospore isolates avirulent or virulent to the Fr1 (fusiform resistance-1) gene were used to inoculate a control-pollinated loblolly pine family heterozygous for this gene. With two avirulent isolates, the regression curve of gall frequency 9 months after inoculation went from 26 to 50% as inoculum density increased to 100 × 103 spores. The regression curve flattened at higher inoculum densities. With two virulent isolates, gall frequency increased from 47% to a plateau at 97% as spore density increased. A double-blind element of the study correlated the occurrence of the genetic marker (RAPD marker J7-485A) for Fr1 resistance in haploid megagametophyte tissuend the presence or absence of galls on seedlings after artificial inoculations. With avirulent isolates at the two higher densities of 100 × 103 and 200 × 103, marker presence-absence and phenotypic assessments of gall presence-absence agreed for 95% of the seedlings. At the 50 ×103 level, marker-phenotype agreed for 86% of the seedlings. The increased marker-phenotype association resulted from a reduction or elimination of disease escapes as Fr1 resistance remained stable even at higher spore densities. The double-blind study indicates that resistant individuals can be identified from the megagametophyte tissue of germinating seedlings. With virulent isolates, marker and disease phenotype did not correlate, even at the lowest inoculum density. The virulent isolates appear to be homozygous for virulence because infection of marker-positive resistant seedlings equaled or exceeded that of marker-negative susceptible seedlings at the lowest inoculum density. }, number={6}, journal={PLANT DISEASE}, author={Kuhlman, EG and Amerson, HV and Jordan, AP and Pepper, WD}, year={1997}, month={Jun}, pages={597–600} }
 @inproceedings{o'malley_grattapaglia_chaparro_wilcox_amerson_liu_whetten_mckeand_kuhlman_mccord_et al._1996, title={Molecular markers,  forest genetics, and tree breeding}, DOI={10.1007/978-1-4899-0280-1_7}, abstractNote={Several years ago, Strauss et al. (1992) thoughtfully evaluated the application of molecular markers in forest tree breeding for marker aided selection. The purpose of their paper was to emphasize the limitations and shortcomings of marker-aided selection particularly in conifers. They argued that studies of quantitative trait loci identified in agronomic crops, which have significant utility (e.g. Stuber, 1992; Stuber et al., 1992), are of little relevance to assessing the potential for marker aided selection in populations of forest trees, and that the near term usefulness of molecular markers for forest tree breeding will be limited. The major barriers to application included cost, the lack of association of markers with traits across breeding populations due to linkage equilibrium, variation in expression of loci affecting quantitative traits due to differences in genetic background, genotype environment interactions, and stability of marker-trait associations over multiple generations. In addition, Strauss et al. (1992) noted that marker-aided selection would be most useful for within family selection, where the economic values of the traits are high, the trait heritabilities are low, and where markers are able to explain much of the genetic variance. However, they argued that important traits in forest trees such as wood volume, are likely to be controlled by large numbers of genes with small effects, and therefore, are unlikely to have useful marker trait associations.}, booktitle={Genomes of Plants and Animals: 21 Stadler Genetics Symposium}, publisher={Plenum Press,  NY}, author={O'Malley, D. M. and Grattapaglia, D. and Chaparro, J. X. and Wilcox, P. L. and Amerson, H. V. and Liu, B-H and Whetten, R. and McKeand, Steven and Kuhlman, E. G. and McCord, S. and et al.}, editor={Gustafson, J. P. and Flavell, R. B.Editors}, year={1996}, pages={87–102} }
 @article{hu_amerson_frampton_1994, title={Isolation and growth of single genotype axenic cultures of Cronartium quercuum f. sp. fusiforme using hyphal fragments from multi-genotype stock colonies}, volume={8}, number={4}, journal={New Forests}, author={Hu, A. and Amerson, H. V. and Frampton, L. J., Jr.}, year={1994}, pages={299–308} }
 @inproceedings{grattapaglia_chaparro_wilcox_mccord_crane_amerson_werner_liu_o'malley_whetten_et al._1993, title={Application of genetic markers to tree breeding}, booktitle={Proceedings of the 22nd Southern Forest Tree Improvement Conference}, author={Grattapaglia, D. and Chaparro, J. and Wilcox, P. and McCord, S. and Crane, B. and Amerson, H. and Werner, D. and Liu, B. H. and O'Malley, D. and Whetten, R. and et al.}, year={1993}, pages={452–463} }
 @inproceedings{grattapaglia_chaparro_wilcox_mccord_werner_amerson_mckeand_bridgwater_whetten_o'malley_et al._1992, title={Mapping in woody plants with RAPD markers: application to breeding in forestry and horticulture}, booktitle={Proceedings of the Symposium on the Applications of RAPD Technology to Plant Breeding}, publisher={Joint Plant Breeding Symposium Series,  Crop Science Society of America, American Society for Horticultural Science, and American Genetics Association}, author={Grattapaglia, D. and Chaparro, J. and Wilcox, P. and McCord, S. and Werner, D. and Amerson, H. and McKeand, S. and Bridgwater, F. and Whetten, R. and O'Malley, D. and et al.}, year={1992}, pages={37–40} }
 @article{hu_amerson_1991, title={SINGLE GENOTYPE AXENIC CULTURES OF CRONARTIUM-QUERCUUM F-SP FUSIFORME}, volume={81}, ISSN={["0031-949X"]}, DOI={10.1094/Phyto-81-1294}, abstractNote={Single genotype axenic cultures of Cronartium quercuum f. sp. fusiforme were isolated from individually cultured haploid basidiospores. Successful single spore initiations were obtained only on Harvey and Grasham (4) basal medium amended with 0.1 g/L CaCO 3 , 1 g/L each of yeast extract and peptone, and 10 g/L of bovine serum albumin (HGYP+BSA). Nurse culture techniques were necessary for significant numbers of successful colony initiations (...)}, number={10}, journal={PHYTOPATHOLOGY}, author={HU, A and AMERSON, HV}, year={1991}, month={Oct}, pages={1294–1297} }
 @book{amerson_frampton_mott_1989, title={Annual progress reports, special project on tissue culture}, institution={Raleigh, NC: Forest Biology Research Center, North Carolina State University}, author={Amerson, H. V. and Frampton, L. J., Jr. and Mott, R. L.}, year={1989} }
 @article{amerson_frampton_mott_spaine_1988, title={Tissue culture of conifers using loblolly pine as a model}, ISBN={0306428156}, DOI={10.1007/978-1-4613-1661-9_7}, journal={Genetic manipulation of woody plants}, publisher={New York: Plenum}, author={Amerson, H. V. and Frampton, L. J. and Mott, R. L. and Spaine, P. C.}, editor={J. W. Hanover and Keathley, D. E.Editors}, year={1988}, pages={117–137} }
 @inproceedings{frampton_amerson_gray_1985, title={Development of in vitro techniques for screening loblolly pine for fusiform rust resistance}, volume={2}, booktitle={Proceedings of the 2nd Rusts of Hard Pines Working Party Conference (Athens, GA)}, publisher={IUFRO and Southeastern Forest Experiment Station}, author={Frampton, L. J., Jr. and Amerson, H. V. and Gray, D. J.}, editor={J. Barrows-Broaddus and Powers, H. R.Editors}, year={1985}, pages={125–140} }
 @inproceedings{frampton_mott_amerson_1985, title={Field performance of loblolly pine tissue culture plantlets}, booktitle={Proceedings of the eighteenth southern forest tree improvement conference held at Long Beach, Mississippi on May 21-23, 1985}, author={Frampton, L. J., Jr. and Mott, R. L. and Amerson, H. V.}, year={1985}, pages={136–144} }
 @inproceedings{amerson_frampton_mott_1985, title={In vitro methods for the study of fusiform rust in association with loblolly pine}, booktitle={Proceedings of the Rusts of Hard Pines Working Party Conference}, publisher={Southeastern Forest Experiment Station}, author={Amerson, H. V. and Frampton, L. J., Jr. and Mott, R. L.}, editor={J. Barrows-Broaddus and Powers, H. R.Editors}, year={1985} }
 @inbook{amerson_mckeand_e._r._weir_1985, title={Loblolly pine tissue culture: laboratory, greenhouse, and field studies}, ISBN={030641919X}, DOI={10.1007/978-1-4899-0378-5_19}, booktitle={Tissue culture in forestry and agriculture}, publisher={NY: Plenum Press}, author={Amerson, H. V. and McKeand, Frampton L. J. and E., Mott S. and R., L. and Weir, R. J.}, editor={R. R. Henke, K. W. Hughes and M. J. Constantin, A. HollaenderEditors}, year={1985}, pages={271–287} }
 @inproceedings{frampton_amerson_weir_1983, title={Potential of in vitro screening of loblolly pine for fusiform rust resistance}, booktitle={Proceedings of the seventeenth southern forest tree improvement conference , June 7-9, 1983.}, author={Frampton, L. J., Jr. and Amerson, H. V. and Weir, R. J.}, year={1983}, pages={325–331} }
 @inproceedings{amerson_mckeand_mott_1981, title={Tissue culture and greenhouse practices for the production of loblolly pine plantlets}, booktitle={Proceedings of the 16th Southern Forest Tree Improvement Conference}, author={Amerson, H. V. and McKeand, S. E. and Mott, R. L.}, year={1981}, pages={168–175} }